scholarly journals Formulasi Sediaan Mikroemulsi Gel Anti Jerawat Mengandung Kombinasi Minyak Jinten Hitam (Nigella sativa L.) dan Minyak Zaitun (Olea europaea L.)

2019 ◽  
Vol 6 (2) ◽  
pp. 57
Author(s):  
Sani Ega Priani ◽  
Wulan Kartika Dewi ◽  
Amila Gadri
Keyword(s):  
Antibacterial Activity ◽  
Olive Oil ◽  
Droplet Size ◽  
Seed Oil ◽  
Propionibacterium Acnes ◽  
Nigella Sativa ◽  
Gelling Agent ◽  
Black Seed ◽  
Black Seed Oil ◽  
Microemulsion Gel

<p align="center"><strong>Abstrak</strong></p><p align="center"><strong> </strong></p><p class="Default">Jerawat adalah penyakit kulit yang paling sering terjadi dan timbul akibat penyumbatan atau inflamasi pada kelenjar pilosebasea. <em>Propionibacterium acnes </em>diketahui merupakan bakteri utama pemicu terjadinya inflamasi pada jerawat. Minyak jinten hitam dan minyak zaitun diketahui memiliki aktivitas antibakteri sehingga dapat dikembangkan untuk pengobatan jerawat karena infeksi. Penelitian ini bertujuan untuk menguji aktivitas antibakteri kombinasi minyak jinten hitam dan minyak zaitun terhadap <em>P. acnes</em> dan memformulasikannya ke dalam bentuk sediaan mikroemulsi gel. Pengujian aktivitas antibakteri dilakukan dengan metode difusi agar pada konsentrasi kombinasi minyak 0,25; 0,5; dan 1%. Formulasi sediaan mikroemulsi gel dilakukan dengan menggunakan <em>cremophor RH </em>40 sebagai surfaktan, gliserin sebagai kosurfaktan, dan viscolam mac 10 sebagai <em>gelling agent</em>. Karakterisasi sediaan meliputi pengujian organoleptis, pH, viskositas, sifat alir, ukuran globul, dan stabilitas termodinamik. Hasil penelitian menunjukkan bahwa konsentrasi minyak 0,5% sudah memberikan aktivitas antibakteri terhadap <em>P. acnes</em> dengan diameter hambat 12,47±1,07mm. Formula optimum mikroemulsi gel adalah formula yang mengandung minyak (6%), <em>cremophor RH </em>40 (35%), gliserin (35%), dan gel viskolam 20%. Sediaan mikroemulsi gel memiliki rata rata ukuran globul 120 nm yang memenuhi persyaratan ukuran globul mikroemulsi. Sediaan mikroemulsi gel stabil berdasarkan hasil uji stabilitas termodinamik dengan tidak terlihat adanya pemisahan fase.</p><p><strong> </strong></p><p><strong>Kata kunci</strong> :   Minyak jinten hitam, Minyak zaitun, <em>P</em><em>.</em><em> acnes</em><em>, </em>Mikroemulsi gel</p><p> </p><p align="center"><strong><em>Formulation of anti acne microemulsion gel containing combination of black seed oil </em></strong><strong><em>(Nigella sativa L.) </em></strong><strong><em> and olive oil </em></strong><strong><em>(Olea europaea L.)</em></strong><strong><em> </em></strong></p><p align="center"><strong><em> </em></strong></p><p align="center"><strong><em>Abstract</em></strong><strong><em></em></strong></p><p align="center"><strong><em> </em></strong></p><p><em>Acne vulgaris is the most common </em><em>skin disease involving blockage and/or inflammation of pilosebaceous unit. </em><em>Propionibacterium acnes</em><em>  has</em><em> been recognized as pus-forming bacteria triggering an inflammation in acne</em><em>. Black seed and olive oil are herbal medicines that known have antibacterial activity, that could be develop for acne treatment. </em><em>This research was conducted to determine antibacterial activity of black seed and olive oil </em><em>combination</em><em> against P. acnes and </em><em>develop the microemulsion gel preparation containing of the oil. </em><em>Antibacterial activity test was conducted by agar diffusion method at concentration at 0,25; 0,5; and 1% of oil combination. </em><em>Microemulsion gel was made using </em><em>cremophor RH 40 as surfactant, </em><em>glycerine as </em><em>cosurfactant, and viscolam mac 10</em><em> as </em><em>gelling agent.  Microemulsion gel was evaluated by organoleptic, pH, rheologycal properties,</em><em> spreadability, droplet size, and  </em><em> thermodinamic  stabilitiy test. The result showed that concentration 0,5% of oil combination</em><em> has antibacterial activity </em><em>towards P. acnes </em><em>with inhibitory diameter </em><em>12,47 </em><em>± 1,069</em><em> </em><em>mm. Optimum formula microemulsion gel with the finest characteristic, containing the oil combination </em><em>(6%), cremophor RH 40  (35%), gliserin  (35%), and  viscolam</em><em> gel </em><em> 20%. The</em><em> average of</em><em> droplet size microemulsion gel is 120</em><em> </em><em>nm and those size are qualify with the droplet size of microemulsion.</em><em> The microemulsion gel was stabile based on </em><em>thermodinamic</em><em> stability</em><em> tests without phase separation.</em><strong><em></em></strong></p><p><strong><em> </em></strong></p><p class="Default"><strong><em>Keywords</em></strong><em>:       black seed oil, olive oil, P. acnes, microemulsion gel</em></p>

scholarly journals Development of self nanoemulsifying drug delivery system for black seed oil (Nigella sativa L.)

2020 ◽  
Vol 1469 ◽  
pp. 012022
Author(s):  
S E Priani ◽  
S S Maulidina ◽  
F Darusman ◽  
L Purwanti ◽  
D Mulyanti
Keyword(s):  
Drug Delivery ◽  
Drug Delivery System ◽  
Delivery System ◽  
Seed Oil ◽  
Nigella Sativa ◽  
Black Seed ◽  
Black Seed Oil

scholarly journals Development, Characterization Optimization, and Assessment of Curcumin-Loaded Bioactive Self-Nanoemulsifying Formulations and Their Inhibitory Effects on Human Breast Cancer MCF-7 Cells

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1107
Author(s):  
Mohsin Kazi ◽  
Fahd A. Nasr ◽  
Omar Noman ◽  
Abdulrahman Alharbi ◽  
Mohammed S. Alqahtani ◽  
...  
Keyword(s):  
Droplet Size ◽  
Mtt Assay ◽  
Oral Delivery ◽  
Seed Oil ◽  
Drug Loading ◽  
Drug Candidate ◽  
Lipid Digestion ◽  
Black Seed ◽  
Black Seed Oil ◽  
Mcf 7

Curcumin (CUR) is an attractive polyphenol for its anti-inflammatory, antibacterial, antioxidant, and anticancer properties. Poor solubility in water and sensitivity against sunlight are the most challenging characteristics in the development of CUR for clinical use. The aim is to develop oral lipid-based bioactive self-nanoemulsifying drug delivery systems (Bio-SNEDDSs) for curcumin as a candidate for cancer therapy. Bio-SNEDDSs containing black seed oil, medium-chain mono- and diglycerides, and surfactants were prepared as CUR delivery vehicles. The morphology, droplet size, physical stability, encapsulation efficiency, risk of precipitation, lipid digestion, antioxidant activity, and antimicrobial activity were evaluated for the representative formulations. Finally, an MTT assay was performed on MCF-7 cells to determine the cytotoxic effect of the different formulations. The results showed lower droplet size (28.53 nm) and higher drug-loading (CUR 20 mg, thymoquinone 1.2 mg) for the representative Bio-SNEDDS (black seed oil/Imwitor 988/KolliphorEL (35/15/50) % w/w), along with a transparent appearance upon aqueous dilution. The dynamic dispersion and in-vitro lipolysis data proved that the Bio-SNEDDS was able to keep the CUR in a solubilized form in the gastrointestinal tract. From the antioxidant and antimicrobial studies, it was suggested that the Bio-SNEDDS had the highest activity for disease control. The MTT assay showed that the representative Bio-SNEDDS treatment led to a reduction of cell viability of MCF-7 cells compared to pure CUR and conventional SNEDDSs. A Bio-SNEDDS with elevated entrapment efficiency, antioxidant/antimicrobial activities, and an antiproliferative effect could be the best anticancer drug candidate for potential oral delivery.

Growth performance, hematological parameters and lipid profile of mice treated with black seed oil and vitamin-E

2021 ◽  
Vol 7 (2) ◽  
pp. 132-137
Author(s):  
Afrina Mustari ◽  
Khaled Mahmud Sujan ◽  
Debasree Sarker Moni ◽  
Mahabub Alam ◽  
Mohammad Alam Miah ◽  
...  
Keyword(s):  
Vitamin E ◽  
Growth Performance ◽  
Seed Oil ◽  
Nigella Sativa ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Black Seed ◽  
Black Seed Oil

Nigella sativa (Black seed) has been recognized as one of the most popular herbs in many parts of the world for centuries and used as folk medicine to cure different kinds of diseases. Vitamin E is well known for its antioxidative and anti-inflammatory properties, and has been studied in the prevention of cardiovascular disease (CVD). The present study was performed to determine the effect of black seed oil and Vit-E on growth performance and blood biochemistry in mice. A total of 40 mice weight between 25-27 gm were used for conducting the research. At first, the mice were randomly divided into 4 groups consisting 10 mice in each group. Group A served as the control group whereas Group B was administered black seed oil @ 0.5 ml/kg/day, Groups C was taken vitamin E @ 200 mg/kg/day and Group D was received both black seed oil @ 0.5 ml/kg/day + vitamin E @ 200 mg/kg/day respectively for 42 days. Result showed that, body weight of B, C and D group revealed a significant decrease than the control group (p<0.05). Hemoglobin concentration (Hb) and Total Erythrocyte Count (TEC) showed a significant change in B, C and D group than the control group (p<0.05). On the other hand, Total Leukocyte Count (TLC) and Packed Cell Volume (PCV) were insignificant. The results of biochemical test explored that serum cholesterol and triglyceride value decreased significantly whereas HDL increased significantly in black seed oil, Vit-E treated groups than the control group (p<0.05). But the LDL showed no change after treatment. It could be concluded that the black seed oil and Vit-E can be used in the therapeutic strategy of obesity, anemia and coronary diseases. Asian J. Med. Biol. Res. 2021, 7 (2), 132-137

Impacts of various mixing approaches towards black seed oil-alginate emulsion attributes

2021 ◽  
Vol 11 (10) ◽  
pp. 1746-1751
Author(s):  
Shaiqah Mohd Rus ◽  
Farahidah Mohamed ◽  
Mohd. Rushdi Abu Bakar ◽  
Abd Almonem Doolaanea ◽  
Awis Sukarni Mohmad Sabere
Keyword(s):  
Zeta Potential ◽  
Droplet Size ◽  
Large Scale ◽  
Seed Oil ◽  
Antioxidant Property ◽  
Dissolution Time ◽  
Black Seed ◽  
Magnetic Stirrer ◽  
Mixing Method ◽  
Black Seed Oil

Black seed oil (BSO) contains thymoquinone, an active ingredient that is well-known for its antioxidant property. The bitter taste of BSO makes it challenging for the consumers, mainly children and the elderly, to consume it every day. Thus, BSO is encapsulated in alginate beads in micrometre size to enhance its palatability. This encapsulation was previously established in the lab-scale and the standard mixing method to produce a stable BSO-alginate emulsion used a magnetic stirrer coupled with a sonicator. This mixing method is not suitable for the production of BSO beads at a large scale. Hence, this research aims to investigate the impacts of various mixing methods coupled with sonication towards the BSO-alginate emulsion attributes. Four BSOalginate coarse emulsions were prepared using a magnetic stirrer (MS) as the benchmark, overhead stirrer (OS), homogeniser (H), and overhead stirrer combined with homogeniser (OSH). All the coarse emulsions were then sonicated to produce a nanoemulsion using a flow cell sonicator. The coarse emulsions were characterised in terms of dissolution time while the nanoemulsions were characterised in terms of droplet size, polydispersity index, zeta potential, conductivity and absorbance. These characteristics were statistically compared to the benchmark emulsion characteristics. MS, OS, H, and OSH coarse emulsion formation required 45, 230, 102, and 46 minutes to be produced, respectively. There were significant differences (p <0.05) between the droplet size of OSH (266.53±4.36) nm and MS (285.50±1.15) nm emulsions, indicating that the OSH emulsion was more stable. The absorbance of OSH BSO-alginate nanoemulsion was significantly lower (p <0.05) than the benchmark. In comparison to MS as the benchmark, OSH technique produced a stable emulsion in terms of lower dissolution time, droplet size, zeta potential, and absorbance.

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