Impacts of various mixing approaches towards black seed oil-alginate emulsion attributes

Black seed oil (BSO) contains thymoquinone, an active ingredient that is well-known for its antioxidant property. The bitter taste of BSO makes it challenging for the consumers, mainly children and the elderly, to consume it every day. Thus, BSO is encapsulated in alginate beads in micrometre size to enhance its palatability. This encapsulation was previously established in the lab-scale and the standard mixing method to produce a stable BSO-alginate emulsion used a magnetic stirrer coupled with a sonicator. This mixing method is not suitable for the production of BSO beads at a large scale. Hence, this research aims to investigate the impacts of various mixing methods coupled with sonication towards the BSO-alginate emulsion attributes. Four BSOalginate coarse emulsions were prepared using a magnetic stirrer (MS) as the benchmark, overhead stirrer (OS), homogeniser (H), and overhead stirrer combined with homogeniser (OSH). All the coarse emulsions were then sonicated to produce a nanoemulsion using a flow cell sonicator. The coarse emulsions were characterised in terms of dissolution time while the nanoemulsions were characterised in terms of droplet size, polydispersity index, zeta potential, conductivity and absorbance. These characteristics were statistically compared to the benchmark emulsion characteristics. MS, OS, H, and OSH coarse emulsion formation required 45, 230, 102, and 46 minutes to be produced, respectively. There were significant differences (p <0.05) between the droplet size of OSH (266.53±4.36) nm and MS (285.50±1.15) nm emulsions, indicating that the OSH emulsion was more stable. The absorbance of OSH BSO-alginate nanoemulsion was significantly lower (p <0.05) than the benchmark. In comparison to MS as the benchmark, OSH technique produced a stable emulsion in terms of lower dissolution time, droplet size, zeta potential, and absorbance.

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Development, Characterization Optimization, and Assessment of Curcumin-Loaded Bioactive Self-Nanoemulsifying Formulations and Their Inhibitory Effects on Human Breast Cancer MCF-7 Cells

Pharmaceutics ◽

10.3390/pharmaceutics12111107 ◽

2020 ◽

Vol 12 (11) ◽

pp. 1107

Author(s):

Mohsin Kazi ◽

Fahd A. Nasr ◽

Omar Noman ◽

Abdulrahman Alharbi ◽

Mohammed S. Alqahtani ◽

...

Keyword(s):

Droplet Size ◽

Mtt Assay ◽

Oral Delivery ◽

Seed Oil ◽

Drug Loading ◽

Drug Candidate ◽

Lipid Digestion ◽

Black Seed ◽

Black Seed Oil ◽

Mcf 7

Curcumin (CUR) is an attractive polyphenol for its anti-inflammatory, antibacterial, antioxidant, and anticancer properties. Poor solubility in water and sensitivity against sunlight are the most challenging characteristics in the development of CUR for clinical use. The aim is to develop oral lipid-based bioactive self-nanoemulsifying drug delivery systems (Bio-SNEDDSs) for curcumin as a candidate for cancer therapy. Bio-SNEDDSs containing black seed oil, medium-chain mono- and diglycerides, and surfactants were prepared as CUR delivery vehicles. The morphology, droplet size, physical stability, encapsulation efficiency, risk of precipitation, lipid digestion, antioxidant activity, and antimicrobial activity were evaluated for the representative formulations. Finally, an MTT assay was performed on MCF-7 cells to determine the cytotoxic effect of the different formulations. The results showed lower droplet size (28.53 nm) and higher drug-loading (CUR 20 mg, thymoquinone 1.2 mg) for the representative Bio-SNEDDS (black seed oil/Imwitor 988/KolliphorEL (35/15/50) % w/w), along with a transparent appearance upon aqueous dilution. The dynamic dispersion and in-vitro lipolysis data proved that the Bio-SNEDDS was able to keep the CUR in a solubilized form in the gastrointestinal tract. From the antioxidant and antimicrobial studies, it was suggested that the Bio-SNEDDS had the highest activity for disease control. The MTT assay showed that the representative Bio-SNEDDS treatment led to a reduction of cell viability of MCF-7 cells compared to pure CUR and conventional SNEDDSs. A Bio-SNEDDS with elevated entrapment efficiency, antioxidant/antimicrobial activities, and an antiproliferative effect could be the best anticancer drug candidate for potential oral delivery.

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Bioactive Self-Nanoemulsifying Drug Delivery Systems (Bio-SNEDDS) for Combined Oral Delivery of Curcumin and Piperine

Molecules ◽

10.3390/molecules25071703 ◽

2020 ◽

Vol 25 (7) ◽

pp. 1703 ◽

Cited By ~ 5

Author(s):

Mohsin Kazi ◽

Ahmad A. Shahba ◽

Saad Alrashoud ◽

Majed Alwadei ◽

Abdelrahman Y. Sherif ◽

...

Keyword(s):

Drug Delivery ◽

Droplet Size ◽

Health Care Professionals ◽

Seed Oil ◽

Amorphous State ◽

In Vitro Dissolution ◽

Size Analysis ◽

Black Seed ◽

Natural Oils ◽

Black Seed Oil

Background: Bioactive oils of natural origin have gained huge interests from health care professionals and patients. Objective: To design a bioactive self-nanoemulsifying drug delivery system (Bio-SNEDDS) comprising curcumin (CUR) and piperine (PP) by incorporating bioactive natural oils in the formulation. Methods: The self-emulsifying properties of apricot, avocado, black seed and Zanthoxylum rhetsa seed oils were screened within various SNEDDS formulations. Each liquid SNEDDS formulation was loaded with both CUR and PP. The optimal liquid SNEDDS were solidified using Aeroperl® and Neusilin® at 1:1 w/w ratio. Liquid and solid SNEDDS were characterized by droplet size analysis, equilibrium solubility, scanning electron microscopy, X-ray powder diffraction, differential scanning calorimetry, and Fourier transform infrared spectroscopy. In-vitro dissolution studies were performed to evaluate the efficiency of CUR and PP release from solid Bio-SNEDDS. Results: The liquid SNEDDS comprised of black seed oil exhibited excellent self-emulsification performance, low droplet size along with transparent appearance. The inclusion of the cosolvent Transcutol P improved the solubilization capacity of both CUR and PP. The liquid SNEDDS were efficiently solidified using the two adsorbents and presented the drugs within amorphous state. In particular, SNEDDS comprised of black seed oil/Imwitor988/Transcutol P/Cremophor RH40 (20/20/10/50) and when solidified with Neusilin showed enhanced CUR and PP release (up to 60% and 77%, respectively). In addition, this formulation efficiently delivers the highly bioactive black seed oil to the patient. Conclusions: The optimized Bio-SNEDDS comprising black seed oil showed outstanding self-emulsification characteristics along with enhanced CUR/PP dissolution upon solidification.

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Formulasi Sediaan Mikroemulsi Gel Anti Jerawat Mengandung Kombinasi Minyak Jinten Hitam (Nigella sativa L.) dan Minyak Zaitun (Olea europaea L.)

Kartika Jurnal Ilmiah Farmasi ◽

10.26874/kjif.v6i2.143 ◽

2019 ◽

Vol 6 (2) ◽

pp. 57

Author(s):

Sani Ega Priani ◽

Wulan Kartika Dewi ◽

Amila Gadri

Keyword(s):

Antibacterial Activity ◽

Olive Oil ◽

Droplet Size ◽

Seed Oil ◽

Propionibacterium Acnes ◽

Nigella Sativa ◽

Gelling Agent ◽

Black Seed ◽

Black Seed Oil ◽

Microemulsion Gel

Abstrak Jerawat adalah penyakit kulit yang paling sering terjadi dan timbul akibat penyumbatan atau inflamasi pada kelenjar pilosebasea. Propionibacterium acnes diketahui merupakan bakteri utama pemicu terjadinya inflamasi pada jerawat. Minyak jinten hitam dan minyak zaitun diketahui memiliki aktivitas antibakteri sehingga dapat dikembangkan untuk pengobatan jerawat karena infeksi. Penelitian ini bertujuan untuk menguji aktivitas antibakteri kombinasi minyak jinten hitam dan minyak zaitun terhadap P. acnes dan memformulasikannya ke dalam bentuk sediaan mikroemulsi gel. Pengujian aktivitas antibakteri dilakukan dengan metode difusi agar pada konsentrasi kombinasi minyak 0,25; 0,5; dan 1%. Formulasi sediaan mikroemulsi gel dilakukan dengan menggunakan cremophor RH 40 sebagai surfaktan, gliserin sebagai kosurfaktan, dan viscolam mac 10 sebagai gelling agent. Karakterisasi sediaan meliputi pengujian organoleptis, pH, viskositas, sifat alir, ukuran globul, dan stabilitas termodinamik. Hasil penelitian menunjukkan bahwa konsentrasi minyak 0,5% sudah memberikan aktivitas antibakteri terhadap P. acnes dengan diameter hambat 12,47±1,07mm. Formula optimum mikroemulsi gel adalah formula yang mengandung minyak (6%), cremophor RH 40 (35%), gliserin (35%), dan gel viskolam 20%. Sediaan mikroemulsi gel memiliki rata rata ukuran globul 120 nm yang memenuhi persyaratan ukuran globul mikroemulsi. Sediaan mikroemulsi gel stabil berdasarkan hasil uji stabilitas termodinamik dengan tidak terlihat adanya pemisahan fase. Kata kunci : Minyak jinten hitam, Minyak zaitun, P. acnes, Mikroemulsi gel Formulation of anti acne microemulsion gel containing combination of black seed oil (Nigella sativa L.) and olive oil (Olea europaea L.) Abstract Acne vulgaris is the most common skin disease involving blockage and/or inflammation of pilosebaceous unit. Propionibacterium acnes has been recognized as pus-forming bacteria triggering an inflammation in acne. Black seed and olive oil are herbal medicines that known have antibacterial activity, that could be develop for acne treatment. This research was conducted to determine antibacterial activity of black seed and olive oil combination against P. acnes and develop the microemulsion gel preparation containing of the oil. Antibacterial activity test was conducted by agar diffusion method at concentration at 0,25; 0,5; and 1% of oil combination. Microemulsion gel was made using cremophor RH 40 as surfactant, glycerine as cosurfactant, and viscolam mac 10 as gelling agent. Microemulsion gel was evaluated by organoleptic, pH, rheologycal properties, spreadability, droplet size, and thermodinamic stabilitiy test. The result showed that concentration 0,5% of oil combination has antibacterial activity towards P. acnes with inhibitory diameter 12,47 ± 1,069 mm. Optimum formula microemulsion gel with the finest characteristic, containing the oil combination (6%), cremophor RH 40 (35%), gliserin (35%), and viscolam gel 20%. The average of droplet size microemulsion gel is 120 nm and those size are qualify with the droplet size of microemulsion. The microemulsion gel was stabile based on thermodinamic stability tests without phase separation. Keywords: black seed oil, olive oil, P. acnes, microemulsion gel

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Preparation and characterization of black seed oil loaded solid lipid nanoparticles for topical formulations: A preliminary study

10.1063/5.0052706 ◽

2021 ◽

Author(s):

Acharee Suksuwan ◽

Zamzam Arour ◽

Najwa Santiworakun ◽

Winai Dahlan

Keyword(s):

Solid Lipid Nanoparticles ◽

Seed Oil ◽

Lipid Nanoparticles ◽

Topical Formulations ◽

Solid Lipid ◽

Black Seed ◽

Preliminary Study ◽

Black Seed Oil

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INVESTIGATION OF THE EFFECT OF BLACK SEED OIL ON DIABETES-INDUCED - TESTICULAR DAMAGE: A HISTOPATHOLOGICAL STUDY

10.36106/ijsr/9045949 ◽

2021 ◽

pp. 29-31

Author(s):

Seval Kaya ◽

Yusuf Nergiz ◽

Firat Asir

Keyword(s):

Tissue Damage ◽

Seed Oil ◽

Histopathological Examination ◽

Male Rats ◽

Seminiferous Tubules ◽

Control Group ◽

Histopathological Study ◽

Diabetes Group ◽

Black Seed ◽

Black Seed Oil

In this study, it was aimed to investigate the protective effect of black seed oil against testicular tissue damage in diabetic rats. A total of 18 male rats were divided into 3 groups, including 6 rats in each group.Groups; control (n=6), diabetes (n=6), diabetes + black seed oil (n=6). A single dose of 45 mg / kg streptozocine (STZ) was injected intraperitoneally to induce diabetes. Diabetes + Black seed oil group: For 56 days, 2.5 ml / kg of black seed oil was administered orally to rats.The rats were sacriced at the end of 56 days. Testicular tissues were taken for routine parafn tissue processing for histopathological examination. Parafn sections were stained with Hematoxylin-Eosin and PAS and examined under a light microscope. Atrophy and degeneration were observed in the seminiferous tubules of diabetic group. Histology of black seed oil group sections were similar to that of control group. A signicant difference was found between the black seed oil group and the diabetes group in terms of blood glucose values. As a result, we think that Black Seed Oil ameliorates to the tissue damage caused by diabetes and the decrease in blood sugar value.

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Protective effect of black seed oil from Nigella sativa against murine cytomegalovirus infection

International Journal of Immunopharmacology ◽

10.1016/s0192-0561(00)00036-9 ◽

2000 ◽

Vol 22 (9) ◽

pp. 729-740 ◽

Cited By ~ 95

Author(s):

Mohamed Labib Salem ◽

Mohammad Sohrab Hossain

Keyword(s):

Protective Effect ◽

Cytomegalovirus Infection ◽

Seed Oil ◽

Nigella Sativa ◽

Murine Cytomegalovirus ◽

Black Seed ◽

Black Seed Oil

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Simple HPLC Method for the Determination of Thymoquinone in Black Seed Oil (Nigella Sativa Linn)

Journal of Liquid Chromatography ◽

10.1080/10826079508010400 ◽

1995 ◽

Vol 18 (5) ◽

pp. 895-902 ◽

Cited By ~ 20

Author(s):

Hassan Y. Aboul-Enein ◽

Laila I. Abou-Basha

Keyword(s):

Seed Oil ◽

Nigella Sativa ◽

Hplc Method ◽

Black Seed ◽

Black Seed Oil

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Effects of Bisphenol-A (BPA) and black seed oil on body weight, lipid profile and serum glucose in male and female mice

10.33109/bjvmjj19lam2 ◽

2019 ◽

Vol 17 (1) ◽

Author(s):

K. M. Sujan ◽

E. Haque ◽

M. S. Rakib ◽

M. I. Haque ◽

A. Mustari ◽

...

Keyword(s):

Body Weight ◽

Weight Gain ◽

Bisphenol A ◽

Lipid Profile ◽

Seed Oil ◽

Serum Glucose ◽

Male And Female ◽

Female Mice ◽

Black Seed ◽

Black Seed Oil

Background: Bisphenol-A [BPA, 2, 2-bis (hydroxyphenyl) propane] is widely used in the manufacture of polycarbonate plastic, water bottles, feeders , baby bottles, epoxy resins and inside coating in metallic food cans. Black seed oil (BSO) (Nigella sativa) commonly known as black cumin, reported to be beneficial in function of various systems in the body. The study was carried out to investigate the effect of BPA and BSO on body weight, lipid profile and serum glucose in male and female mice. Methods : A total of thirty (15 male and 15 female) Swiss Albino mice (Mus musculus), aged 25-28 days with an average body weight of 27.4±1g were randomly divided into 3 groups consisting 5 mice in each for each sex. Group A served as vehicle control. Group B was administered BPA @ 50 mg/kg bw daily, while group C received both BPA @ 50 mg/kg/day and BSO @ 1ml/kg/day respectively. Results: Data revealed that BPA treated mice showed slight increase in body weight gain while BSO controlled the weight gain in BPA treated mice. Cholesterol and LDL values were significantly (p<0.01) increased and Triglycerides value was significantly (p<0.01) decreased in BPA-treated mice without significant alterations in HDL value. BPA & BSO treated female mice showed significant (p<0.01) decreased in cholesterol, triglycerides and LDL values. BPA reduced the blood glucose level and addition of BSO had synergistic effects of glucose utilization. Conclusions: It can be concluded that BPA is one of the potential risk factors for hyperlipidemia and obesity. These harmful effects could be alleviated by the ingestion of black seed oil.

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