Adenylate Nucleotides and 2,3-Biphosphoglycerate Concentration in Erythrocytes of Growing Wielkopolska Stallions

The aim of this study was to examine the relationships between the concentrations of adenylate nucleotides (ATP, ADP, AMP), total nucleotide pool (TAN), adenylate energy charge (AEC) and 2,3-biphosphoglycerate (2,3-BPG) in the erythrocytes of young horses in the period of their rapid growth and development. The studies were conducted on 10 young Wielkopolska breed stallions for two years; Group A: 1-month-old, Group B: 3-month-old, Group C: 6-month-old, Group D: 1-year-old, and Group E: 2-year-old. ATP, ADP and AMP concentrations in the erythrocytes were determined by HPLC. 2,3-BPG was determined using the Calbiochem USA kit. We observed that erythrocyte ATP, ADP, AMP and TAN concentrations significantly decreased with age (p < 0.001), while 2,3-BPG concentration and AEC values significantly increased with age. A significant correlation between the ATP and TAN concentrations was found within all the examined groups (p < 0.05). A significant correlation was also found between ATP concentration and AEC values (except 12 month-old stallions). Moreover, an age-dependent negative correlation between ATP and 2,3-BPG (r = -0.81) was found. The conclusion is that the adenylate energy charge and erythrocyte 2,3-BPG variables in the Wielkopolska stallions mainly depend on age, thus warranting the use of specific age-related reference values for this breed's foals.

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Microscopic Study on the Diameter of the Pineal Calcification of Bangladeshi Cadavers

Bangladesh Journal of Anatomy ◽

10.3329/bja.v9i2.15220 ◽

2013 ◽

Vol 9 (2) ◽

pp. 71-74

Author(s):

Halima Afroz ◽

Abu Sadat Mohammad Nurunnabi ◽

Shamim Ara ◽

Mushfika Rahman ◽

Tahamida Yesmin ◽

...

Keyword(s):

Histological Study ◽

Age Groups ◽

Cross Sectional ◽

Medical College ◽

Age Related ◽

Microscopic Features ◽

Group A ◽

Group B ◽

Group D ◽

Age Related Changes

Context: The human pineal gland is characterized by the presence of calcified concretions, called pineal acervuli or brain sand. These are basophilic extracellular bodies. The study was carried out to describe the microscopic features of pineal calcifications and to find out age related changes. Study Design: Cross sectional descriptive type of study. Place and period of study: Department of Anatomy, Dhaka Medical College, Dhaka, from July 2009 to June 2010. Materials: 60 postmortem human pineal glands were collected from unclaimed dead bodies that were under examination in the morgue of Department of Forensic Medicine, Dhaka Medical College, Dhaka. Methods: The samples were divided into four different age groups, i.e. Group-A (15-30 years), Group-B (31-40 years), Group-C (41-50 years) and Group-D (>50 years). Histological study was carried out on relatively 27 fresh samples. Results: The mean diameter of the pineal calcifications were 351.14±111.69 ?m in group A, 600.00±232.69 ?m in group B, 909.43±124.18 ?m in group C and 1541.67±224.54 ?m in group D. The differences in diameter of the pineal calcifications between group A & C, A & D, B & C, B & D and C & D were statistically significant. Conclusion: Age related changes were found in the diameter of pineal calcifications. DOI: http://dx.doi.org/10.3329/bja.v9i2.15220 Bangladesh Journal of Anatomy 2011 Vol.9(2) pp.71-74

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Microscopic study on the shape of pineal calcification of Bangladeshi cadavers

Journal of Dhaka Medical College ◽

10.3329/jdmc.v22i2.21526 ◽

2015 ◽

Vol 22 (2) ◽

pp. 151-155 ◽

Cited By ~ 1

Author(s):

Halima Afroz ◽

Abu Sadat Mohammad Nurunnabi ◽

Shamim Ara ◽

Mushfika Rahman ◽

Hosna Ara Perven

Keyword(s):

Pineal Gland ◽

Irregular Shape ◽

Medical College ◽

Age Related ◽

Microscopic Features ◽

Group A ◽

Bangladeshi Population ◽

Group B ◽

Group D ◽

Age Related Changes

Context: The degree of calcification increases from younger to older in human pineal gland and the morphology of pineal calcifications change with advancing age as well. The aim of the present study was to describe the microscopic features of the pineal calcifications and find out age related changes in a Bangladeshi population. Methods: A descriptive type of study was done in the Department of Anatomy, Dhaka Medical College, from July 2009 to June 2010, based on the collection of 60 human pineal glands from whole human brains of unclaimed dead bodies that were under postmortem examination in the Department of Forensic Medicine, Dhaka Medical College, Dhaka. After collection of the pineal gland, those were divided into group A (15-45 years), group B (31-40 years), group C (41-50 years) and group D (>50 years). All the histological slides from different groups were observed under the light compound microscope and their shapes were noted down. Results: In group A, 53.33% were found to be round, 40% oval and 6.67% of irregular shape. In group B, there were 45.46% round, 40.90% oval and 13.64% of irregular shape. In group C, there were 75% of irregular shape, 21.47% mulberry shaped and 3.53% oval. In group D, there were 80.65% mulberry shaped and 19.35% of irregular shaped. Conclusion: Age related changes were found in morphological features of pineal calcification. In young adults, the shape of the pineal calcification remains round or oval. With increasing age, it becomes irregular and mulberry shaped. DOI: http://dx.doi.org/10.3329/jdmc.v22i2.21526 J Dhaka Medical College, Vol. 22, No.2, October, 2013, Page 151-155

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Microscopic study on the total number of follicles of ovary in Bangladeshi female cadavers

Update Dental College Journal ◽

10.3329/updcj.v3i1.17979 ◽

2014 ◽

Vol 3 (1) ◽

pp. 12-17

Author(s):

Rubina Qasim ◽

Humaira Naushaba ◽

Md Enayet Ullah ◽

Hasna Hena ◽

Laila Farzana Khan

Keyword(s):

Age Groups ◽

Cross Sectional ◽

Group 13 ◽

Age Related ◽

Group A ◽

The Mean ◽

Group 5 ◽

Group B ◽

The Right ◽

Group D

Context: The ovaries are paired female reproductive and endocrine glands. It is an unique organ in the female body. It undergoes various changes under the influence of hormones which started from prepubertal period and continue up to post menopausal period. Reproductive life span is determined by the histological changes of ovarian follicles. The study was carried out to counting the total number of follicles in relation to age. Study design: Cross sectional, descriptive type of study. Place and period of study: Department of Anatomy, Sir Salimullah Medical College (SSMC), Dhaka from July 2010 to June 2011. Materials: 65 ovaries of Bangladeshi female cadavers age ranging from 5 years to 65 years. Methods: The samples were divided into four different age groups. They were group A or prepubertal group (5-12years), group B or reproductive group (13-45 years), group C or perimenopausal group (46-51years) and group D or postmenopausal group (52-65years). Histological studies were carried out only in right ovary on 24 fresh samples, 6 samples from each group. RESULTS: The mean (±SD) total number of the follicles in the right ovaries were 82.67±2.07, 43.33±8.17, 10.00± 5.47 and 1.17±1.94 per square millimeter in group A, B, C & D respectively. The differences of the mean (±SD) number of the follicles were highly significant (P<0.001) among the all age groups and was significant (P<0.01) when compared between group C with group D. CONCLUSION: Age related changes were observed in the total number of follicles of the ovary. DOI: http://dx.doi.org/10.3329/updcj.v3i1.17979 Update Dent. Coll. j: 2013; 3 (1): 12-17

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Age related volume of cadaver-prostates in Bangladesh

Ibrahim Medical College Journal ◽

10.3329/imcj.v4i2.6501 ◽

1970 ◽

Vol 4 (2) ◽

pp. 74-77

Author(s):

Rukshana Ahmed ◽

Shamim Ara

Keyword(s):

Prostate Gland ◽

Age Groups ◽

Clinical Settings ◽

Cross Sectional ◽

Medical College ◽

Age Related ◽

Group A ◽

Key Words Prostate ◽

The Mean ◽

Group B

Pathological changes in the prostate gland occur commonly with advancing age including inflammation, atrophy, hyperplasia and carcinoma and a change in volume is also evident. Estimation of volume of prostate may be useful in a variety of clinical settings. A cross-sectional descriptive study was designed to see the changes in volume of the prostate with advancing age and done in the Department of Anatomy, Dhaka Medical College, Dhaka from August 2006 to June 2007. The study was performed on 70 post-mortem human prostates collected from the unclaimed dead bodies that were under examination in the Department of Forensic Medicine, Dhaka Medical College, Dhaka. The samples were divided into three age groups; group A (10-20 years), group B (21-40 years) and group C (41-70 years). Volume of the sample was measured by using the ellipsoid formula. The mean ± SD volume of prostate was 7.68 ± 3.64 cm3 in group A, 10.61 ± 3.99 cm3 in group B and 15.40 ± 6.31 cm3 in group C. Mean difference in volume between group A and group C, group B and group C were statistically significant (p<0.001). Statistically significant positive correlation was found between age and volume of prostate (r = + 0.579, p < 0.001). Key Words: Prostate; volume; Bangladeshi. DOI: 10.3329/imcj.v4i2.6501Ibrahim Med. Coll. J. 2010; 4(2): 74-77

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Survival of classic swine fever virus in hams made from the meat of pigs vaccinated with the PAV-250 strain and unvaccinated pigs

Revista Mexicana de Ciencias Pecuarias ◽

10.22319/rmcp.v10i3.4528 ◽

2019 ◽

Vol 10 (3) ◽

pp. 536-551

Author(s):

Heidi Amezcua Hempel ◽

María Salud Rubio Lozano ◽

Eliseo Manuel Hernández Baumgarten ◽

Pablo Correa Girón † ◽

Oscar Torres Ángeles ◽

...

Keyword(s):

Reference Strain ◽

Clinical Signs ◽

Classical Swine Fever ◽

Fever Virus ◽

Direct Immunofluorescence ◽

Biometric Analysis ◽

Treatment Groups ◽

Group A ◽

Group B ◽

Group D

The study was to determine the presence of Classical Swine Fever virus (CSFv), in the meat of vaccinated pigs with the PAV-250 strain and then challenged using the same strain. Five treatment groups were established (each with four pigs). Group A: Pigs thatwere fed with processed hams from negative animals; Group B: Pigs that were fed with processed hams from commercial pigs inoculated with the ALD (reference strain) (titre of 104.0/ml); Group C: Pigs fed with processed hams from pigs infected with the virulent ALD strain (titre of 102.5/ml); Group D: Pigs fed with processed hams from pigs vaccinated with the PAV-250 strain and challenged with the ALD strain (titre of 101.1/ml); and Group E: Pigs fed with processed hams from pigs vaccinated with two doses of the PAV-250 strain and challenged with the ALD strain (negative). Blood samples were taken at d 1, 5, 10, 15 and 20 for biometric analysis. Groups B, C and D manifested clinical signs of CSFv: 40 °C temperature, anorexia, paralysis, vomiting, diarrhea, tremor, hirsute hair and cyanosis. Pigs were slaughtered and necropsies performed to identify lesions in tissues. Results of direct immunofluorescence testing of tissues were positive and the virus was recovered. Under these study conditions, it was found that CSFv resisted the cooking method at 68 °C for 40 min in hams from unvaccinated pigs, and that the virus was able to transmit the disease to healthy unvaccinated pigs, whereas the hams from the vaccinated animals did not transmit the virus.

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The Role of Obesity in Predicting the Clinical Outcomes of COVID-19

Obesity Facts ◽

10.1159/000517180 ◽

2021 ◽

pp. 1-9

Author(s):

Serdar Sahin ◽

Havva Sezer ◽

Ebru Cicek ◽

Yeliz Yagız Ozogul ◽

Murat Yildirim ◽

...

Keyword(s):

Clinical Outcomes ◽

Health Care Providers ◽

Study Groups ◽

Normal Weight ◽

Overweight And Obesity ◽

Care Providers ◽

Significant Difference ◽

Group A ◽

Group B ◽

Group D

Introduction: The aim of this was to describe the predictors of mortality related to COVID-19 infection and to evaluate the association between overweight, obesity, and clinical outcomes of COVID-19. Methods: We included the patients >18 years of age, with at least one positive SARS-CoV-2 reverse transcriptase-polymerase chain reaction. Patients were grouped according to body mass index values as normal weight <25 kg/m2 (Group A), overweight from 25 to <30 kg/m2 (Group B), Class I obesity 30 to <35 kg/m2 (Group C), and ≥35 kg/m2 (Group D). Mortality, clinical outcomes, laboratory parameters, and comorbidities were compared among 4 groups. Results: There was no significant difference among study groups in terms of mortality. Noninvasive mechanical ventilation requirement was higher in group B and D than group A, while it was higher in Group D than Group C (Group B vs. Group A [p = 0.017], Group D vs. Group A [p = 0.001], and Group D vs. Group C [p = 0.016]). Lung involvement was less common in Group A, and presence of hypoxia was more common in Group D (Group B vs. Group A [p = 0.025], Group D vs. Group A [p < 0.001], Group D vs. Group B [p = 0.006], and Group D vs. Group C [p = 0.014]). The hospitalization rate was lower in Group A than in the other groups; in addition, patients in Group D have the highest rate of hospitalization (Group B vs. Group A [p < 0.001], Group C vs. Group A [p < 0.001], Group D vs. Group A [p < 0.001], Group D vs. Group B [p < 0.001], and Group D vs. Group C [p = 0.010]). Conclusion: COVID-19 patients with overweight and obesity presented with more severe clinical findings. Health-care providers should take into account that people living with overweight and obesity are at higher risk for COVID-19 and its complications.

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AB0422 LEFT VENTRICULAR ABNORMALITIES IN SYSTEMIC LUPUS ERYTHEMATOSUS PATIENTS FOLLOWED BY SEQUENTIAL ECHOCARDIOGRAPHY

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2020-eular.1941 ◽

2020 ◽

Vol 79 (Suppl 1) ◽

pp. 1510.1-1511

Author(s):

T. Kuga ◽

M. Matsushita ◽

K. Tada ◽

K. Yamaji ◽

N. Tamura

Keyword(s):

Lupus Erythematosus ◽

Systolic Dysfunction ◽

Left Ventricular ◽

Monocyte Count ◽

Lv Dysfunction ◽

Group A ◽

Group B ◽

Lv Diastolic Dysfunction ◽

Group D

Background:Cardiovascular disease (CVD) is detected in up to 50% of systemic lupus erythematosus (SLE) patients1and major cause of death2. Even clinically silent SLE patients can develop left ventricular (LV) diastolic dysfunction3. Proper echocardiographic follow up of SLE patients is required.Objectives:To clarify how the prevalence of LV abnormalities changes over follow-up period and identify the associated clinical factors, useful in suspecting LV abnormalities.Methods:29 SLE patients (24 females and 5 men, mean age 52.8±16.3 years, mean disease duration 17.6±14.5 years) were enrolled. All of them underwent echocardiography as the baseline examination and reexamined over more than a year of follow-up period(mean 1075±480 days) from Jan 2014 to Sep 2019. Patients complicated with pulmonary artery hypertension, deep venous thrombosis or pulmonary embolism and underwent cardiac surgery during the follow-up period were excluded. Left ventricular(LV) systolic dysfunction was defined as ejection fraction (EF) < 50%. LV diastolic dysfunction was defined according to ASE/EACVI guideline4. LV dysfunction (LVD) includes one or both of LV systolic dysfunction and LV diastolic function. Monocyte to HDL ratio (MHR) was calculated by dividing monocyte count with HDL-C level.Prevalence of left ventricular abnormalities was analysed at baseline and follow-up examination. Clinical characteristics and laboratory data were compared among patient groups as follows; patients with LV dysfunction (Group A) and without LV dysfunction (Group B) at the follow-up echocardiography, patients with LV asynergy at any point of examination (Group C) and patients free of LV abnormalities during the follow-up period (Group D).Results:At the baseline examination, LV dysfunction (5/29 cases, 13.8%), LV asynergy (6/29 cases, 21.7%) were detected. Pericarditis was detected in 7 patients (24.1%, LVD in 3 patients, LV asynergy in 2 patients) and 2 of them with subacute onset had progressive LV dysfunction, while 5 patients were normal in echocardiography after remission induction therapy for SLE. At the follow-up examination, LV dysfunction (9/29 cases, 31.0%, 5 new-onset and 1 improved case), LV asynergy (6/29 cases, 21.7%, 2 new-onset and 2 improved cases) were detected. Though any significant differences were observed between Group A and Group B at the baseline, platelet count (156.0 vs 207.0, p=0.049) were significantly lower in LV dysfunction group (Group A) at the follow-up examination. Group C patients had significantly higher uric acid (p=0.004), monocyte count (p=0.009), and MHR (p=0.003) than Group D(results in table).Conclusion:LV dysfunction is progressive in most of patients and requires regular follow-up once they developed. Uric acid, monocyte count and MHR are elevated in SLE patients with LV asynergy. Since MHR elevation was reported as useful marker of endothelial dysfunction5, our future goal is to analyse involvement of monocyte activation and endothelial dysfunction in LV asynergy of SLE patients.References:[1]Doria A et al. Lupus. 2005;14(9):683-6.[2]Manger K et al. Ann Rheum Dis. 2002 Dec;61(12):1065-70.[3]Leone P et al. Clin Exp Med. 2019 Dec 17.[4]Nagueh SF et al. J Am Soc Echocardiogr. 2016 Apr;29(4):277-314.[5]Acikgoz N et al. Angiology. 2018 Jan;69(1):65-70.Numbers are median (interquartile range), Mann-Whitney u test were performed, p value less than 0.05 was considered statistically significant.Disclosure of Interests: :None declared

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Prophylactic temporary abdominal aortic balloon occlusion for patients with pernicious placenta previa: a retrospective study

BMC Anesthesiology ◽

10.1186/s12871-021-01354-1 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Fei Huo ◽

Hansheng Liang ◽

Yi Feng

Keyword(s):

Placenta Previa ◽

Balloon Occlusion ◽

Artery Embolism ◽

Abdominal Aortic ◽

Significant Difference ◽

Group A ◽

Group B ◽

Clinical Records ◽

Group D ◽

Central Placenta Previa

Abstract Background Pernicious placenta previa (PPP) can increase the risk of perioperative complications. During caesarean section in patients with adherent placenta, intraoperative blood loss, hysterectomy rate and transfusion could be reduced by interventional methods. Our study aimed to investigate the influence of maternal hemodynamics control and neonatal outcomes of prophylactic temporary abdominal aortic balloon (PTAAB) occlusion for patients with pernicious placenta previa. Methods This was a retrospective study using data from the Peking University People’s Hospital from January 2014 through January 2020. Clinical records of pregnant women undergoing cesarean section were collected. Patients were divided into two groups: treatment with PTAAB placement (group A) and no balloon placement (group B). Group A was further broken down into two groups: prophylactic placement (Group C) and balloon occlusion (group D). Results Clinical records of 33 cases from 5205 pregnant women underwent cesarean section were collected. The number of groups A, B, C, and D were 17, 16, 5 and 12.We found that a significant difference in the post-operative uterine artery embolism rates between group A and group B (0% vs.31.3%, p = 0.018). There was a significant difference in the Apgar scores at first minute between group A and group B (8.94 ± 1.43 vs 9.81 ± 0.75,p = 0.037),and the same significant difference between two groups in the pre-operative central placenta previa (29.4% vs. 0%,p = 0.044), complete placenta previa (58.8% vs 18.8%, p = 0.032),placenta implantation (76.5% vs 31.3%, p = 0.015). We could also observe the significant difference in the amount of blood cell (2.80 ± 2.68vs.10.66 ± 11.97, p = 0.038) and blood plasma transfusion (280.00 ± 268.32 vs. 1033.33 ± 1098.20, p = 0.044) between group C and group D. The significant differences in the preoperative vaginal bleeding conditions (0% vs 75%, p = 0.009), the intraoperative application rates of vasopressors (0% vs. 58.3%, p = 0.044) and the postoperative ICU (intensive care unit) admission rates (0% vs. 58.3%, p = 0.044) were also kept. Conclusions PTAAB occlusion could be useful in reducing the rate of post-operative uterine artery embolism and the amount of transfusion, and be useful in coping with patients with preoperative vaginal bleeding conditions, so as to reduce the rate of intraoperative applications of vasopressors and the postoperative ICU (intensive care unit) admission. In PPP patients with placenta implantation, central placenta previa and complete placenta previa, we advocate the utilization of prophylactic temporary abdominal aortic balloon placement.

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Biomechanical study of Tile C3 pelvic fracture fixation using an anterior internal system combined with sacroiliac screws

Journal of Orthopaedic Surgery and Research ◽

10.1186/s13018-021-02348-y ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Lin Liu ◽

Donggui Zeng ◽

Shicai Fan ◽

Yongxing Peng ◽

Hui Song ◽

...

Keyword(s):

Minimally Invasive ◽

Steel Plate ◽

Standing Position ◽

Pelvic Fractures ◽

Internal Fixator ◽

Sacroiliac Joints ◽

Sacroiliac Screws ◽

Group A ◽

Group B ◽

Group D

Abstract Background How to perform minimally-invasive surgery on Tile C pelvic fractures is very difficult, and it is also a hot topic in orthopedic trauma research. We applied minimally-invasive treatment using an anterior internal fixator combined with sacroiliac screws. Objectives To compare the biomechanical properties of different fixation models in pelvic facture specimens, using an internal fixation system or a steel plate combined with sacroiliac screws. Methods Sixteen fresh adult cadaver pelvic specimens were randomly separated into four groups named A, B, C, and D. The four groups were respectively stabilized using a two-screwed, three-screwed, or four-screwed anterior internal fixator or a steel plate with sacroiliac screws. All models were tested in both standing and sitting positions. Vertical loads of 600 N were applied increasingly. Shifts of bilateral sacroiliac joints and pubis rupture were measured. Results The shifts in sacroiliac joints and pubis rupture in the standing position were all less than 3.5 mm, and the shifts in the sitting position were all less than 1 mm. In the standing position, the results of shifts in the sacroiliac joints were group C < group D < group B < group A. For comparisons between A:B and C:D, P > 0.05. For comparisons between A, B:C, and D, P < 0.05. The results of shifts in pubis ruptures were group D < group C < group B < group A. In the comparison between C:D, P > 0.05; for comparisons between A:B, A:C, A:D, B:C, and B:D, P < 0.05. In the sitting posture, the results of shifts in the sacroiliac joints were group C < group D < group B < group A, and the shifts in the pubis ruptures were group D < group C < roup B < group A. For comparison between C:D, P > 0.05. For comparisons between A:B, A:C, A:D, B:C, and B:D, P < 0.05. Conclusion Use of an anterior internal fixator combined with sacroiliac screws effectively stabilized Tile C3 pelvic fractures. The stability of specimens increased as the number of screws in the internal fixator increased.

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Hematological Features in Sheep with IgG and IgM Antibodies against Borrelia burgdorferi sensu lato

Pathogens ◽

10.3390/pathogens10020164 ◽

2021 ◽

Vol 10 (2) ◽

pp. 164

Author(s):

Labrini V. Athanasiou ◽

Victoria M. Spanou ◽

Eleni G. Katsogiannou ◽

Panagiotis D. Katsoulos

Keyword(s):

Borrelia Burgdorferi ◽

Rural Areas ◽

Blood Analysis ◽

Borrelia Burgdorferi Sensu Lato ◽

Igm Antibodies ◽

Group A ◽

Group B ◽

Group D ◽

Immunofluorescence Antibody

Exposure of sheep to Borreliaburgdorferi sensulato (s.I.) complex, the causative agent of Lyme borreliosis (LB), has been reported in tick-abundant areas worldwide, while no data have been reported in Greece. The aim of the study was to identify the hematological alterations in sheep with seropositivity against Borrelia burgdorferi (s.I.). Blood samples were obtained from 318 tick infested sheep for blood analysis and serological determination of IgG and IgM antibodies against B. burgdorferi by indirect immunofluorescence antibody (IFA) assay after exclusion of endo-ectoparasites and other tick-borne infections. A total number of 162 sheep met the inclusion criteria, allocated in four groups based on the presence or absence of IgG and/or IgM; sheep found negative for IgM and IgG (Group A), positive for IgM (Group B), positive for both IgM and IgG (Group C) and positive for IgG (Group D). Anemia, thrombocytopenia and normal or decreased leukocyte count, mainly due to lymphopenia were the main hematological features observed in seropositive sheep. The presence of these features raises the suspicion of Borrelia infection in tick infested sheep. The seropositivity of 23.58% in sheep raises concerns of Borrelia circulation, especially in rural areas and potential risk of transmission to humans.

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