Factors Affecting the Content of Ergosterol and Zearalenone in Selected Grass Species at the End of the Growing Season

The objective of the study was to assess the safety of forage prepared from selected grass species by means of the analysis of ergosterol and zearalenone. Graminaceous plants studied at the end of their growing season were Festulolium, Dactylis glomerata and Arrhenatherum elatius. Other indicators assessed were the content of ergosterol and zearalenone during the autumn and the intensity of grassland use in the summer season as related to the studied contents of metabolites. Grasslands were harvested either in October, November or December. The secondary metabolites were analyzed by means of high performance liquid chromatography. At the end of the growing season, the ergosterol content was the lowest in the Festulolium forage (110.04 mg kg-1), the highest contents were observed in the Dactylis glomerata and Arrhenatherum elatius forages (145.73 mg kg-1 and 139.55 mg kg-1, respectively). The safety of Festulolium was also corroborated by the low zearalenone content (0.357 mg kg-1). On the other hand, the high ergosterol content in Arrhenatherum elatius was combined with a high content of zearalenone (1.554 mg kg-1). Although Dactylis glomerata exhibited an ergosterol content comparable with that of Arrhenatherum elatius forage, its zearalenone content was comparable to the Festulolium forage. Among the three species under study we found a significant difference (P < 0.01) in the ergosterol and zearalenone contents. Changes in the ergosterol and zearalenone contents were also apparent from October to December. The highest (P < 0.01) content of ergosterol was found in December (254.26 mg kg-1). The higher (P < 0.01) zearalenone content in this month (1.588 mg kg-1) was caused by the Arrhenatherum elatius forage. Moreover, the contents of ergosterol and zearalenone were affected (P < 0.01) also by the number of cuts in summer. The higher ergosterol content indicated a higher forage infestation by fungi and the inherent risk of the occurrence of mycotoxins. However, the high ergosterol content not always correlated with the high content of zearalenone. When cattle is grazing in winter, a higher occurrence of mycotoxins in the feed may be expected and the related damage to animal metabolism may increase the number of diseased animals with consequent impact on animal production, performance and health and animal husbandry economics.

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Fibre and ergosterol contents in forage of Arrhenatherum elatius, Dactylis glomerata and Festulolium at the end of the growing seaso

Czech Journal of Animal Science ◽

10.17221/346-cjas ◽

2008 ◽

Vol 53 (No. 8) ◽

pp. 320-328 ◽

Cited By ~ 2

Author(s):

J. Skládanka ◽

V. Dohnal ◽

A. Ježková

Keyword(s):

Dry Matter ◽

Dactylis Glomerata ◽

Growing Season ◽

Hplc Method ◽

Ergosterol Content ◽

Arrhenatherum Elatius

The objective of this paper is to evaluate the contents of CF, NDF, ADF and ergosterol in the forage of Fistulolium, Dactylis glomerata and Arrhenatherum elatius from stands harvested at the end of the growing season. The contents of CF, NDF and ADF were analysed using the ANKOM Fibre Analyzer instrument. The ergosterol content was analysed by the HPLC method. The lowest CF content was in the Festulolium forage matter (26.8%). Conversely, the highest content was in the Arrhenatherum elatius forage (30.2%). The CF content gradually increased during autumn from 28.0% to 29.4%. Likewise, the NDF and ADF contents were lowest in Festulolium (58.3% and 32.3%, respectively). The highest NDF content was in Arrhenatherum elatius (60.5%). The ADF contents in Arrhenatherum elatius and Dactylis glomerata were comparable (35.9% and 35.1%, respectively). The NDF content increased during autumn from 56.4% to 62.0% and ADF content from 32.8% to 36.4%. The ergosterol content suggests a lower infestation of the Festulolium forage by fungi. While this content in the Festulolium forage was 94.9 mg/kg of dry matter (DM), in the Dactylis glomerata forage it was 136.7 mg/kg DM and in the Arrhenatherum elatius forage 139.9 mg/kg DM. Forage samples taken in December contained ergosterol on a level of 248.6 mg/kg DM. The species under study and the time of use in autumn exhibited a statistically highly significant effect (P < 0.01) on the contents of CF, NDF, ADF and ergosterol in the forage matter.

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Root mass vertical distribution of perennial cool-season grasses grown in pure or mixed swards

Ciência Rural ◽

10.1590/0103-8478cr20210242 ◽

2022 ◽

Vol 52 (7) ◽

Author(s):

Valentina Ylluyanka Méndez Moncada ◽

Luana Fidelis Américo ◽

Paulo Gonçalves Duchini ◽

Gabriela Cristina Guzatti ◽

Daniel Schmitt ◽

...

Keyword(s):

Soil Conservation ◽

Festuca Arundinacea ◽

Root Biomass ◽

Dactylis Glomerata ◽

Grass Species ◽

Conservation Practices ◽

Root Mass ◽

Soil Environment ◽

Cool Season ◽

Arrhenatherum Elatius

ABSTRACT: In this study we tested whether the root biomass of mixtures composed by grass species is greater than their respective monocultures. The treatments were monocultures of Arrhenatherum elatius, Festuca arundinacea, Dactylis glomerata, and a mixture of them, cultivated in a rich-soil environment. Root biomass was evaluated on a single evaluation per season at three soil depths (0-5, 5-10, and 10-20 cm). Mixed swards presented the greatest root biomass, and this was explained by a greater concentration in the topsoil layer (0-5 cm). These findings reinforce the need for permanent soil conservation practices to not jeopardize the benefits of the more abundant root biomass reported in the mixed swards.

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The effect of some herbicides on seed yield of six grass species – Short Communication

Plant Protection Science ◽

10.17221/4817-pps ◽

2012 ◽

Vol 38 (No. 1) ◽

pp. 23-28

Author(s):

J. Macháč ◽

B. Cagaš ◽

R. Macháč

Keyword(s):

Seed Yield ◽

Short Communication ◽

Poa Pratensis ◽

Dactylis Glomerata ◽

Phleum Pratense ◽

The Other ◽

Grass Species ◽

Festuca Rubra ◽

Festuca Pratensis ◽

Arrhenatherum Elatius

The tolerance to or phytotoxicity of selected herbicides and their impact on seed yield was investigated in six grasses grown for seed: Phleum pratense cv. Sobol (a), Festuca rubra cv. Táborská (b), Festuca pratensis cv. Otava (c), Poa pratensis cv. Slezanka (d), Arrhenatherum elatius cv. Medián (e) and Dactylis glomerata cv. Zora (f). All species were tolerant to Grodyl 75 WG (amidosulfuron 75%), (a) and (f) were tolerant to Grodyl plus (amidosulfuron 15 g/kg + isoproturon 600 g/kg) which was phytotoxic to the other grass species; all species were tolerant to Stomp 330 E (pendimethalin 330 g/l); Lintur 70 WG (triasulfuron 4.1% + dicamba 65.9%) was phytotoxic only to (c) while the other species were tolerant to it.

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Can Festulolium, Dactylis glomerata and Arrhenatherum elatius be used for extension of the autumn grazing season in Central Europe?

Plant Soil and Environment ◽

10.17221/91/2010-pse ◽

2010 ◽

Vol 56 (No. 10) ◽

pp. 488-498 ◽

Cited By ~ 7

Author(s):

J. Skládanka ◽

V. Adam ◽

P. Ryant ◽

P. Doležal ◽

Z. Havlíček

Keyword(s):

Nutritive Value ◽

Dactylis Glomerata ◽

Growing Season ◽

Growth Time ◽

Crude Fibre ◽

Grazing Season ◽

Resistant Species ◽

Harvest Dates ◽

Arrhenatherum Elatius

The paper compares the quality of Festulolium, Dactylis glomerata and Arrhenatherum elatius at the end of the growing season over three years with the contrasting weather in the Czech Republic. The effect of pre-utilisation dates in June or July on the quality of grass forage in October, November and December (harvest dates) was investigated. In the first two years, Arrhenatherum elatius exhibited higher yields (P < 0.05) than Festulolium and Dactylis glomerata. In all treatments, differences between the species and between the years were inconsistent for organic matter digestibility (OMD) and neutral detergent fibre (NDF) but Festulolium showed the lowest crude protein (CP) in all years and crude fibre (CF) in the first 2 years. The longer re-growth time due to earlier final cut in summer increased the yield and decreased the nutritive value in winter. Yields of DM, OMD and CP content generally declined and the CF content increased with the delay of winter harvest. Significant species harvest date interactions in some years were due to the steep increase of CF and NDF in Festulolium. Based on DM yields and nutritive value, all species have some merits as forage for use in possibly extended grazing season. Festulolium and Dactylis glomerata could be used to extend the grazing period in continental conditions. Arrhenatherum elatius had sufficient quality at both dry and warm end of the growing season due to the fact that Arrhenatherum elatius was a representative of drought-resistant species; however, intense grazing could suppress it.

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Seasonal Changes in Leaf Tissue Rehydration of One Annual and Two Perennial Grass Forage Species Induced by Bioclimate

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha43210057 ◽

2015 ◽

Vol 43 (2) ◽

pp. 479-487

Author(s):

Eirini - Ia KAPSALI ◽

Maria KARATASSIOU

Keyword(s):

Water Potential ◽

Water Content ◽

Perennial Grass ◽

Dactylis Glomerata ◽

Leaf Tissue ◽

Growing Season ◽

Grass Species ◽

Forage Species ◽

Relative Water ◽

Dactylis Glomerata L

Bioclimate signifies the continuous interplay between plants and climate factors (primarily drought) and has a direct impact on the water relations and the duration of the rehydration process in water stressed plants. To explore the association between bioclimate and water physiology of forage species in semi-arid Mediterranean grasslands, we determined the seasonal variation in leaf water potential, turgid weight and relative water content in wild growing Dactylis glomerata L., Bromus inermis Leyss (perennial) and Bromus sterilis L. (annual) during the growing season. The study was conducted at the farm of the Aristotle University of Thessaloniki. The results of the current study reveal that B. sterilis maintained high levels of water potential most probably by accelerating its biological cycle and decreasing water content because it fails to sustain turgidity. Dactylis glomerata and B. inermis presented even higher water contents than B. sterilis for the same water potential. Dactylis glomerata exhibited substantially higher water potential and content than B. inermis by keeping the rehydration duration stable. The extensive creeping rhizome seems to allow B. inermis to sustain high values of water potential and content possibly ensuring turgidity. Regardless of the grass species the duration of rehydration ranged from 2.5 to 3.5 hours throughout the growing season. Our findings demonstrate that (a) D. glomerata and B. inermis are better adapted to Mediterranean semiarid conditions than B. sterilis and (b) turgid weight in Mediterranean forage species can safely be determined after a rehydration period of 3.5 hours.

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Effect of Supplementation of Multi Enzymes on Production Performance and Egg Quality Traits in White Leghorn Layers

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.v12i2.3730 ◽

2016 ◽

Vol 12 (2) ◽

Author(s):

C. Pandian ◽

A. Sundaresan ◽

A. V. Omprakash

Keyword(s):

Egg Production ◽

Egg Quality ◽

Live Weight ◽

Economic Benefits ◽

Production Performance ◽

Feed Consumption ◽

Quality Traits ◽

White Leghorn ◽

Significant Difference ◽

Enzyme Supplementation

The present study was conducted to assess the effect of supplementation of Multi-enzymes with lysophospholipids on production performance of pure line White Leghorn layers. Body weights before and after the experiment did not differ significantly across the experimental diets. Irrespective of the dietary treatments, the birds gained 3.83 per cent of live weight relative to its initial body weight. Mean per cent Hen housed egg production was significantly (Pis less than 0.05) higher in diet supplemented with 0.10 MEC-L than other groups. Mean egg weight and average daily feed consumption during 25 to 35 weeks of age indicated no significant effect of enzyme supplementation. Average daily feed consumption per bird in control, 0.05 % and 0.1% multi-enzyme supplemented groups was 108.13, 105.66 and 107.67 g respectively and birds offered control diet recorded numerically more feed intake than enzyme supplemented groups. Comparatively low feed per egg was observed in 0.10 per cent group followed by 0.05 per cent group which offers economic benefits than control diets. However, the egg quality traits between different dietary enzyme supplementation groups showed no significant difference.

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Metabolomic analysis reveals the interaction of primary and secondary metabolism in white, pale green, and green pak choi (Brassica rapa subsp. chinensis)

Applied Biological Chemistry ◽

10.1186/s13765-020-00574-2 ◽

2021 ◽

Vol 64 (1) ◽

Author(s):

Hyeon Ji Yeo ◽

Seung-A Baek ◽

Ramaraj Sathasivam ◽

Jae Kwang Kim ◽

Sang Un Park

Keyword(s):

Secondary Metabolites ◽

Brassica Rapa ◽

High Performance ◽

Reducing Power ◽

Total Phenolic ◽

Pak Choi ◽

Primary And Secondary Metabolites ◽

Chlorophyll Contents ◽

Significant Difference ◽

Pale Green

AbstractThis study aimed to comprehensively analyze primary and secondary metabolites of three different-colored (white, pale green, and green) pak choi cultivars (Brassica rapa subsp. chinensis) using gas chromatography attached with time-of-flight mass spectrometry (GC-TOFMS) and high-performance liquid chromatography (HPLC). In total, 53 primary metabolites were identified and subjected to partial least-squares discriminant analysis. The result revealed a significant difference in the primary and secondary metabolites between the three pak choi cultivars. In addition, 49 hydrophilic metabolites were detected in different cultivars. Total phenolic and glucosinolate contents were highest in the pale green and green cultivars, respectively, whereas total carotenoid and chlorophyll contents were highest in the white cultivar. Superoxide dismutase activity, 2,2-diphenyl-1-picrylhydraz scavenging, and reducing power were slightly increased in the white, pale green, and green cultivars, respectively. In addition, a negative correlation between pigments and phenylpropanoids was discovered by metabolite correlation analysis. This approach will provide useful information for the development of strategies to enhance the biosynthesis of phenolics, glucosinolates, carotenoids, and chlorophyll, and to improve antioxidant activity in pak choi cultivars. In addition, this study supports the use of HPLC and GC-TOFMS-based metabolite profiling to explore differences in pak choi cultivars.

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The Effects of Production Performance Model and Competitive Environment of Hospital Systems

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.300-301.1623 ◽

2013 ◽

Vol 300-301 ◽

pp. 1623-1627

Author(s):

Ching Kuo Wei

Keyword(s):

Performance Model ◽

Production Performance ◽

Competitive Environment ◽

Data Envelopment ◽

Hospital System ◽

Department Of Health ◽

Competitive Environments ◽

Significant Difference ◽

Efficiency Performance ◽

Hospital Systems

This study investigated the efficiency performance of the production technology of the Department of Health (DOH)-affiliated hospital system in Taiwan in different competitive environments. This study used Data Envelopment Analysis (DEA) to analyze a total of 396 hospitals of different systems in Taiwan. The results indicated that, in terms of the internal competitive environment, the production performance of hospital Q was the best, while that of hospital N was the worst. This study also analyzed the production performance and scale of DOH-affiliated hospitals and provided hospitals with a direction for scale development. Finally, this study proposed suggestions on improvement direction for hospitals with poor production performance. As for external competitive environment, there is no significant difference in the average efficiency among various hospital systems. However, there are a lot to be improved in DOH-affiliated hospitals, especially in the aspect of technology efficiency. The improvement of technology efficiency should be more beneficial to the overall efficiency.

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Host plant recognition by the root feeding clover weevil, Sitona lepidus (Coleoptera: Curculionidae)

Bulletin of Entomological Research ◽

10.1079/ber2004317 ◽

2004 ◽

Vol 94 (5) ◽

pp. 433-439 ◽

Cited By ~ 17

Author(s):

S.N. Johnson ◽

P.J. Gregory ◽

P.J. Murray ◽

X Zhang ◽

I.M. Young

Keyword(s):

Subterranean Clover ◽

Trifolium Subterraneum ◽

Plant Roots ◽

Grass Species ◽

Movement Rates ◽

Sitona Lepidus ◽

Macro Scale ◽

Significant Difference ◽

Invasive Techniques ◽

Root Feeding

AbstractThis study investigated the ability of neonatal larvae of the root-feeding weevil, Sitona lepidus Gyllenhal, to locate white clover Trifolium repens L. (Fabaceae) roots growing in soil and to distinguish them from the roots of other species of clover and a co-occurring grass species. Choice experiments used a combination of invasive techniques and the novel technique of high resolution X-ray microtomography to non-invasively track larval movement in the soil towards plant roots. Burrowing distances towards roots of different plant species were also examined. Newly hatched S. lepidus recognized T. repens roots and moved preferentially towards them when given a choice of roots of subterranean clover, Trifolium subterraneum L. (Fabaceae), strawberry clover Trifolium fragiferum L. (Fabaceae), or perennial ryegrass Lolium perenneL. (Poaceae). Larvae recognized T. repens roots, whether released in groups of five or singly, when released 25 mm (meso-scale recognition) or 60 mm (macro-scale recognition) away from plant roots. There was no statistically significant difference in movement rates of larvae.

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Evaluation of the Interference of Hemoglobin Variant J-Bangkok on Glycated Hemoglobin (HbA1c) Measurement by Five Different Methods

Experimental and Clinical Endocrinology & Diabetes ◽

10.1055/s-0043-118535 ◽

2017 ◽

Vol 125 (10) ◽

pp. 655-660

Author(s):

Dong-Mei Wen ◽

Sheng-Nan Xu ◽

Wei-Jia Wang ◽

Xiu-Ming Zhang ◽

Ming-Huan Suo ◽

...

Keyword(s):

High Performance ◽

Glycated Hemoglobin ◽

Clinical Laboratory ◽

Hemoglobin Variant ◽

Deming Regression ◽

Significant Difference ◽

The Difference ◽

Immuno Assay ◽

Hba1c Measurement ◽

Significant Interference

Abstract Objective The interference of the hemoglobin variant (Hb J-Bangkok) was evaluated on 4 different glycated hemoglobin assays and compared with a reference immuno assay. Methods An overall test of coincidence of 2 least-squares linear regression lines was performed to determine whether the presence of Hb J-Bangkok caused a statistically significant difference in HbA1c results compared with a reference immuno assay. Statistical analysis was performed on the difference of the estimated average glucose calculated from HbA1c values and fasting plasma glucose in the Hb J-Bangkok variant group using the different detection systems. Deming regression analysis was used to determinate whether Hb J-Bangkok had a significant interference on HbA1c results using an HbA1c±10% relative bias at 6% and 9% HbA1c as evaluation limits. Results Turbidimetric inhibition immunoassay method, and enzymatic methods were not affected by Hb J-Bangkok. However, Hb J-Bangkok showed statistically significant interference to the two ion-exchange high-performance liquid chromatography methods. Conclusion When performing HbA1c tests, clinical laboratory personnel should identify the Hb variant and select the appropriate methods or use alternative indicators.

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