scholarly journals Rapid diagnostic testing of toxigenic microfungi isolated from foodstuffs

2016 ◽  
Vol 85 (2) ◽  
pp. 151-156 ◽  
Author(s):  
Marie Jefremova ◽  
Vladimír Ostrý ◽  
František Malíř ◽  
Jiří Ruprich

The aim of this study was to experimentally test chromogenic media and reagents for a rapid diagnosis of toxigenic microfungi Aspergillus flavus, A. carbonarius, A. ochraceus, Penicillium expansum, P. verrucosum, and P. citrinum isolated from foodstuffs. Toxigenic microfungi of Aspergillus and Penicillium species are considered to be of major relevance for human and animal health. The presence of these toxigenic microfungi in foodstuffs and feedstuffs raises a potential risk of mycotoxins (for example presence of aflatoxins, cyclopiazonic acid, ochratoxin A, patulin or citrinin). In order to reduce the risks for consumers’ health, it is necessary to set rapid methods for toxigenic microfungi isolated from foodstuffs and also feedstuffs. Application of chromogenic media and reagents presents one of the possibilities. The selected strains of toxigenic microfungi, producing mycotoxins, were tested on chromogenic media. The chromogenic media and reagents in our study are suitable for a microbiological and mycological laboratory. The benefits of chromogenic media and reagents include a rapid diagnosis within 48–72 h, simple preparation, easy availability and relatively low prices.

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2098
Author(s):  
Wen-Yang Chuang ◽  
Li-Jen Lin ◽  
Hsin-Der Shih ◽  
Yih-Min Shy ◽  
Shang-Chang Chang ◽  
...  

With the increase in world food demand, the output of agricultural by-products has also increased. Agricultural by-products not only contain more than 50% dietary fiber but are also rich in functional metabolites such as polyphenol (including flavonoids), that can promote animal health. The utilization of dietary fibers is closely related to their types and characteristics. Contrary to the traditional cognition that dietary fiber reduces animal growth, it can promote animal growth and maintain intestinal health, and even improve meat quality when added in moderate amounts. In addition, pre-fermenting fiber with probiotics or enzymes in a controlled environment can increase dietary fiber availability. Although the use of fiber has a positive effect on animal health, it is still necessary to pay attention to mycotoxin contamination. In summary, this report collates the fiber characteristics of agricultural by-products and their effects on animal health and evaluates the utilization value of agricultural by-products.


2016 ◽  
Vol 51 (10) ◽  
pp. 815-822 ◽  
Author(s):  
Angel Heyerly ◽  
Ron Jones ◽  
Gordon Bokhart ◽  
Mary Shoaff ◽  
Douglas Fisher

2020 ◽  
Vol 58 (10) ◽  
pp. e245-e246
Author(s):  
Shaun S. Tan ◽  
Sharon Saw ◽  
Gabriel Yan ◽  
Ai Teng Chong ◽  
Zhixin Yang ◽  
...  

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 41 ◽  
Author(s):  
André Brito ◽  
Yu Zang

Lignans are polyphenolic compounds with a wide spectrum of biological functions including antioxidant, anti-inflammatory, and anticarcinogenic activities, therefore, there is an increasing interest in promoting the inclusion of lignan-rich foods in humans’ diets. Flaxseed is the richest source of the lignan secoisolariciresinol diglucoside—a compound found in the outer fibrous-containing layers of flax. The rumen appears to be the major site for the conversion of secoisolariciresinol diglucoside to the enterolignans enterodiol and enterolactone, but only enterolactone has been detected in milk of dairy cows fed flaxseed products (whole seeds, hulls, meal). However, there is limited information regarding the ruminal microbiota species involved in the metabolism of secoisolariciresinol diglucoside. Likewise, little is known about how dietary manipulation such as varying the nonstructural carbohydrate profile of rations affects milk enterolactone in dairy cows. Our review covers the gastrointestinal tract metabolism of lignans in humans and animals and presents an in-depth assessment of research that have investigated the impacts of flaxseed products on milk enterolactone concentration and animal health. It also addresses the pharmacokinetics of enterolactone consumed through milk, which may have implications to ruminants and humans’ health.


PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0188047 ◽  
Author(s):  
Amanda VanSteelandt ◽  
Josephine Aho ◽  
Kristyn Franklin ◽  
Jacques Likofata ◽  
Jean Baptiste Kamgang ◽  
...  

2021 ◽  
Vol 1 ◽  
pp. e1236
Author(s):  
Alejandro M. Hasslocher-Moreno ◽  
Ingebourg Georg ◽  
Luiz H. C. Sangenis ◽  
Mauro F. F. Mediano

Introduction: Chagas disease (CD) is a neglected tropical disease. In the chronic phase of CD, the diagnosis is essentially serologic. Conventional reactions are currently in use. More recently, the use of rapid diagnostic testing (RDT) is indicated when conventional techniques are not available. Objective: To evaluate the sensitivity and specificity of RDTs for chronic CD diagnosis. Methodology: Individuals under suspicion of CD were evaluated using ELISA, Chemiluminescence (ChLIA) and RDT tests. Results: The RDT showed 95.1% sensitivity and 96.7% specificity, respectively. Conclusion: The findings of the present study showed that RDT used in the diagnosis of CD at a referral center in Brazil were not able to detect all CD cases when compared to Elisa and ChLIA.


2003 ◽  
Vol 8 (10) ◽  
pp. 876-883 ◽  
Author(s):  
Clinton K. Murray ◽  
David Bell ◽  
Robert A. Gasser ◽  
Chansuda Wongsrichanalai

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Maya Beganovic ◽  
Tristan T Timbrook ◽  
Sarah M Wieczorkiewicz

Abstract Antimicrobial stewardship (AMS) programs integrated with rapid diagnostic tests optimize patient outcomes and reduce time to effective therapy (TTET) and time to optimal therapy (TTOT). This study identifies predictors of TTET and TTOT among patients with positive blood cultures and identifies limitations to current TTOT definitions and outcomes.


2018 ◽  
Vol 4 (1) ◽  
pp. 12-15 ◽  
Author(s):  
Rapeeporn Wongkanya ◽  
Tippawan Pankam ◽  
Shauna Wolf ◽  
Supanit Pattanachaiwit ◽  
Jureeporn Jantarapakde ◽  
...  

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