scholarly journals Matrix metalloproteinases 2 and 9 activity after intra-articular injection of autologous platelet-rich plasma for the treatment of osteoarthritis in dogs

2018 ◽  
Vol 87 (2) ◽  
pp. 127-135 ◽  
Author(s):  
Mustafa Arican ◽  
Atilla Şimşek ◽  
Kurtuluş Parlak ◽  
Kamil Atli ◽  
Gonca Sönmez
Keyword(s):  
Synovial Fluid ◽  
Matrix Metalloproteinase ◽  
Platelet Rich Plasma ◽  
Gelatin Zymography ◽  
Brief Pain Inventory ◽  
Experimental Period ◽  
Matrix Metalloproteinase 2 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Metalloproteinase 9

Intra-articular injection of platelet-rich plasma offers a promising potential for treatment of osteoarthritis in dogs. Twenty dogs weighing 25 to 50 kg (mean 38 kg) with unilateral stifle osteoarthritis were used for the study. Fourteen dogs were given intra-articular platelet rich plasma treatment and 6 dogs were used as controls. Double centrifuge method was used to obtain platelet-rich plasma. Radiography and ultrasonography of the affected joint were carried out and scores for lameness severity and pain severity were assigned by the attending clinicians. Synovial fluid was collected under sterile conditions at pre-treatment and on the 1st, 3rd, 5th, 7th, 15thdays, and 4, 8 and 12 weeks after treatment. Gelatin zymography and Enzyme-linked Immunosorbent Assay were used to determine the synovial fluid levels of matrix metalloproteinase-2 (MMP-2) and metalloproteinase-9 (MMP-9). No adverse effects associated with the injection of the platelet concentrate or saline were observed. Hudson Visual Analog Scale and Canine Brief Pain Inventory scores for all components were non-significantly different between weeks 0, 4, 12 for control dogs. Matrix metalloproteinase-9 was totally and MMP-2 was partially inhibited in the platelet-rich plasma group. In the control group, MMP-9 was partially inhibited during the first month and activation started later. Matrix metalloproteinase-2 was constant in control samples throughout the experimental period. Platelet-rich plasma is a safe and effective method for treatment of dogs with osteoarthritis, possibly more useful for early cases with mild and moderate osteoarthritis. It is suggested that plasma rich platelet should be injected several times at regular intervals instead of a single application.

scholarly journals Gelatinases Increase in Bleomycin-induced Systemic Sclerosis Mouse Model

2019 ◽  
Author(s):  
Fatemeh Vafashoar ◽  
Kazem Mousavizadeh ◽  
Hadi Poormoghim ◽  
Abbas Tavasoli ◽  
Tahereh Musavi Shabestari ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Mouse Model ◽  
Matrix Metalloproteinase ◽  
Gelatin Zymography ◽  
Gelatinolytic Activity ◽  
Matrix Metalloproteinase 2 ◽  
Control Group ◽  
Tissue Sections ◽  
Tissue Homogenates ◽  
And Control

Systemic sclerosis is a fibrotic autoimmune disease in which aberrant remodeling of the extracellular matrix in organs disturbs their functionalities. The aim of this study was to investigate the expression of gelatinases on systemic sclerosis. Consequently, a mouse model of systemic sclerosis was employed and the gelatinolytic activity of gelatinases was evaluated on the fibrotic tissues of this model. Two groups of ten mice were considered in this work: a group of systemic sclerosis model and control group. For the generation of systemic sclerosis model, mice received bleomycin, while the control group was subjected to phosphate buffered saline (PBS) reception. Mice were tested for fibrosis by using trichrome staining, hydroxyproline measurement and α-SMA detection in tissue sections. Additionally, the gelatinolytic activity of matrix metalloproteinase 2 and matrix metalloproteinase 9 were measured using gelatin zymography in lungs and skin tissue homogenates. The obtained results indicated that subcutaneous injection of bleomycin-induced fibrosis in skin and lung tissues of mice. Pro and active forms of matrix methaloproteinase 9 were increased in fibrotic lung tissues (p<0.05 and p<0.01, respectively), while, the gelatinolytic activity of MMP2 was unaffected in these tissues. Additionally, in skin tissues of bleomycin-treated animals, both pro and active forms of MMP9 and MMP2 were increased (p<0.05). Pro and active forms of gelatinases increase differently in skin and lung tissues of bleomycin-induced scleroderma.  

scholarly journals Effect of inflammatory marker activity after intra-articular injection of autologous platelet-rich plasma in dogs with osteoarthritis

2019 ◽  
Vol 75 (08) ◽  
pp. 6285-2019
Author(s):  
MUSTAFA ARICAN ◽  
ATILLA ŞIMŞEK ◽  
KURTULUŞ PARLAK ◽  
KAMIL ATLI ◽  
GONCA SÖNMEZ
Keyword(s):  
Treatment Group ◽  
Synovial Fluid ◽  
Platelet Rich Plasma ◽  
Inflammatory Marker ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Tnf Α ◽  
Autologous Platelet Rich Plasma ◽  
Pg E2 ◽  
Autologous Platelet

Osteoarthritis (OA) is a common cause of lameness, pain, and dysfunction in dogs. To determine the levels of interleukin (IL) (IL-1β, IL-6, IL-10), tumor necrosis factor (TNF)-α, and prostaglandin E2 (PG-E2) in the synovial fluid after intra-articular injection of an autologous platelet-rich plasma concentrate to treat dogs with osteoarthritis. Twenty dogs with osteoarthritis (OA) were used as a materials. Fourteen dogs were used as a platelet-rich plasma (PRP) treatment group and 6 dogs were used as a control (0.9 % NaCl). PRP was obtained by the double centrifuge method. Affected joints were examined by radiography and ultrasonography. Lameness and pain severity were evaluated by attending clinicians. Samples were collected under sterile conditions at pre-treatment, days 1, 3, 5, 7, 15, and weeks 4, 8, and 12. The marker levels were determined by an enzyme-linked immunosorbent assay. No adverse effects of the injection of platelet concentrate or saline solution were observed. No significant differences were found in the Hudson Visual Analog Scale and Canine Brief Pain Inventory scores between weeks 0, 4, and 12 in the control dogs. No significant changes were observed in IL-1β, IL-6, IL-10, TNF-α, and PG-E2 levels (P>0.05) in synovial fluid from the PRP treatment group compared to synovial fluid from the control group, although fluctuations in parameter levels were observed in both groups. Despite some variable results in inflammatory parameters, clinical improvement was recorded in the PRP-treated group. PRP injection could be an effective and safe method for treatment of dogs with osteoarthritis.

scholarly journals Increased activity and expression of matrix metalloproteinase-9 in a rat model of distal colitis

2003 ◽  
Vol 284 (1) ◽  
pp. G116-G122 ◽  
Author(s):  
Carlos Medina ◽  
Sebastián Videla ◽  
Anna Radomski ◽  
Marek W. Radomski ◽  
María Antolín ◽  
...  
Keyword(s):  
Matrix Metalloproteinases ◽  
Matrix Metalloproteinase ◽  
Rat Model ◽  
Therapeutic Potential ◽  
Matrix Metalloproteinase 9 ◽  
Matrix Metalloproteinase 2 ◽  
Control Group ◽  
Plain Water ◽  
Increased Activity ◽  
Metalloproteinase 9

Matrix metalloproteinases may play a role in tissue remodelling and destruction associated with inflammation. We investigated activity and expression of matrix metalloproteinases in a rat model of colitis and tested the therapeutic potential of a synthetic inhibitor (CGS-27023-A). Colitis was induced by dextran sulphate sodium (at 5% in drinking water for 5 days) in a group of eight rats, whereas a matched control group received plain water. Activity and expression of matrix metalloproteinases were measured in colonic tissue homogenates using zymography and Western blot on days 3 and 5 after induction of colitis. In another set of experiments, two groups of colitic rats (20 per group) were treated with CGS-27023-A (20 mg/kg) or vehicle, respectively. On days 5 and 14, colonic mucosal lesions were blindly scored by microscopic examination. Induction of colitis led to a significant upregulation of matrix metalloproteinase-9 protein and its activity, but no change in matrix metalloproteinase-2 activity was observed. Treatment with CGS-27023-A significantly decreased the extent and severity of epithelial injury but did not influence mucosal repair. We conclude that increased activity of matrix metalloproteinases may contribute to epithelial damage in this model of colitis.

How matrix metalloproteinase (MMP)-9 (rs3918242) polymorphism affects MMP-9 serum concentration and associates with autism spectrum disorders: A case-control study in Iranian population

2021 ◽  
pp. 1-7
Author(s):  
Javid Rezaei Lord ◽  
Farhad Mashayekhi ◽  
Zivar Salehi
Keyword(s):  
Serum Concentration ◽  
Matrix Metalloproteinase ◽  
Serum Levels ◽  
Autism Spectrum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mmp9 Expression ◽  
Relationship Of ◽  
The Relationship ◽  
Metalloproteinase 9 ◽  
Control Study

Abstract The aim of this project was to evaluate the relationship of matrix metalloproteinase-9 (MMP-9) genetic variation and its serum concentration with autism spectrum disorder (ASD). One hundred ASD and 120 controls were enrolled in this study. Genomic DNA was extracted from blood and MMP-9 polymorphism was determined by polymerase chain reaction restriction fragment length polymorphism and serum levels were measured by enzyme-linked immunosorbent assay. The frequencies of CC, CT, and TT genotypes were 72%, 26%, and 2% in controls and 31%, 57%, and 12% in ASD, respectively. The frequencies of C and T alleles in ASD were 59.5% and 40.5%, and controls were 86% and 14%, respectively. There is a significant increase in serum MMP-9 levels in ASD as compared to controls. We have also shown that TT genotype is significantly associated with increase serum MMP-9 levels in patients (TT, CT, and CC serum levels were 91.77 ± 10.53, 70.66 ± 7.21, and 38.66 ± 5.52 and in controls were 55.55 ± 11.39, 42.66 ± 7.85, and 30.55 ± 6.34 ng/ml, respectively). It is concluded that there is a significant association between rs3918242 MMP-9 polymorphism and its serum concentration with autism. We also suggest that TT genotype is associated with increased MMP9 expression and may be a risk factor for ASD.

Matrix Metalloproteinase-9 is Involved in the Fibrotic Process in Denervated Muscles after Sciatic Nerve Trauma and Recovery

2021 ◽  
Author(s):  
Oleksii Goncharuk ◽  
Serhii Savosko ◽  
Artem Tykhomyrov ◽  
Mykhailo Guzyk ◽  
Volodymyr Medvediev ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Matrix Metalloproteinase ◽  
Skeletal Muscles ◽  
Gelatin Zymography ◽  
Matrix Metalloproteinase 9 ◽  
Nerve Reconstruction ◽  
Fibrotic Process ◽  
Collagen Density ◽  
Denervated Muscles ◽  
Metalloproteinase 9

AbstractFibrosis of the injured muscles is a problem of recovery from trauma and denervation. The aim of the work was to investigate the interconnection of matrix metalloproteinase-9 (ММР-9) activity in denervated muscles with fibrosis and to estimate its role in nerve restoration by the epineurial suture, fibrin-based glue, and polyethylene glycol hydrogel. The activity of matrix metalloproteinases was estimated by gelatin zymography. Collagen density in muscles was determined histochemically. An increased level of the active MMP-9 is associated with the fibrous changes in the denervated skeletal muscles and after an epineurial suture. The use of fibrin glue and polyethylene glycol hydrogel resulted in a lower level of collagen and ММР-9 activity, which may be a therapeutic target in the treatment of neuromuscular lesions, and has value in fibrosis analysis following microsurgical intervention for peripheral nerve reconstruction.

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