Identification of tuna species Thunnus albacares and Katsuwonus pelamis in canned products by real-time PCR method

Tuna species are a popular food among consumers. They are mostly sold as heat-processed canned products on the market. Different quality and price of tuna species can lead the producer to the adulteration of food products. The main difficulties in developing a method for species identification in these fish is the high similarity of DNA sequences among close relative fish species. All complete mitochondrial DNA sequences of skipjack tuna (Katsuwonus pelamis) and yellowfin tuna (Thunnus albacares) were compared to all other mitochondrial DNA sequences of tuna fish deposited in the GenBank. The most variable regions within species were determined and primers and probes were designed in this region for the species-specific DNA amplification of skipjack tuna and yellowfin tuna. Moreover, to check the content of amplifiable DNA of fish (namely tuna) in the sample, primers and a probe of mitochondrial 12S rRNA gene in the region of conservative sequence were designed. Real time PCR methods were verified by investigating 51 samples of canned tuna with the declared content of tuna species from the market; the species was confirmed in all tested samples. This method was designed to be suitable for the determination of DNA sequences especially in highly heat treated products.

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Quantitative Detection of Species-Specific DNA in Feedstuffs and Fish Meals

Journal of Food Protection ◽

10.4315/0362-028x-68.6.1217 ◽

2005 ◽

Vol 68 (6) ◽

pp. 1217-1221 ◽

Cited By ~ 27

Author(s):

PAVEL KRCMAR ◽

EVA RENCOVA

Keyword(s):

Mitochondrial Dna ◽

Real Time ◽

Dna Sequences ◽

Real Time Pcr ◽

Single Species ◽

Absolute Quantification ◽

Quantitative Detection ◽

Vertebrate Species ◽

Target Species ◽

Species Specific

A sensitive and rapid method for the quantitative detection of bovine-, ovine-, swine-, and chicken-specific mitochondrial DNA sequences based on real-time PCR has been developed. The specificity of the primers and probes for real-time PCR has been tested using DNA samples of other vertebrate species that may also be present in rendered products. The quantitative detection was performed with dual-labeled probes (TaqMan) using absolute quantification with external standards of single species meat-and-bone meals. This method facilitates the detection of 0.01% of the target species–derived material in concentrate feed mixtures and fish meals.

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STATUS PERIKANAN HUHATE (POLE AND LINE) DI BITUNG, SULAWESI UTARA

Jurnal Penelitian Perikanan Indonesia ◽

10.15578/jppi.14.3.2008.313-320 ◽

2017 ◽

Vol 14 (3) ◽

pp. 313 ◽

Cited By ~ 1

Author(s):

Budi Nugraha ◽

Enjah Rahmat

Keyword(s):

Yellowfin Tuna ◽

Fishing Ground ◽

Thunnus Albacares ◽

Skipjack Tuna ◽

Katsuwonus Pelamis ◽

Coryphaena Hippurus ◽

North Sulawesi ◽

The Status ◽

Catch Composition ◽

First Maturity

Tulisan ini menyajikan tentang status perikanan huhate di Bitung meliputi deskripsi unit penangkapan, daerah penangkapan, komposisi hasil tangkapan, catch per unit of effort, dan ukuran ikan pertama kali tertangkap. Data dikumpulkan selama tahun 2004 sampai dengan 2005. Hasil penelitian menunjukkan bahwa huhate yang terdapat di Bitung dioperasikan dengan kapal penangkapan yang terbuat dari kayu berukuran 50 sampai dengan 80 GT. Daerah penangkapan di sekitar lokasi rumpon di Laut Sulawesi dan Laut Maluku. Hasil tangkapan yang diperoleh terdiri atas cakalang (Katsuwonus pelamis), madidihang (Thunnus albacares), baby tuna (Thunnus spp.), dan tongkol (Auxis spp.) serta hasil tangkapan sampingan yaitu lemadang (Coryphaena hippurus) dan sunglir (Elagatis bipinnulatus). Hasil analisis catch per unit of effort diperoleh bahwa nilai catch per unit of effort baby tuna (Thunnus spp.) mengalami kenaikan pada bulan Agustus 2004, dan cakalang (Katsuwonus pelamis) mengalami kenaikan pada bulan September 2004. Hasil analisis terhadap ukuran pertama kali cakalang (Katsuwonus pelamis) tertangkap oleh huhate 49,3 FLcm. Ukuran ini lebih panjang dibandingkan ukuran pertama kali cakalang (Katsuwonus pelamis) matang gonad. Sedangkan hasil analisis terhadap ukuran pertama kali madidihang (Thunnus albacares) tertangkap oleh huhate 51,6 FLcm. Ukuran ini lebih pendek dibandingkan ukuran pertama kali madidihang (Thunnus albacares) matang gonad. This paper presents the status of pole and line fishery in Bitung of North Sulawesi, consisting of description of fishing gear, fishing ground, catch composition, catch per unit of effort, and length at first capture. Data were collected during the period of 2004 until 2005. Results show that the pole and line in Bitung operated by wooden vessels of 50 until 80 GT. The fishing grounds were the waters around FADs location in Sulawesi Sea and Maluku Sea. Catch composition consists of skipjack tuna (Katsuwonus pelamis), yellow fin tuna (Thunnus albacares), baby tuna (Thunnus spp.), and frigate tuna (Auxis spp.), while the bycatch consisted of dolphinfish (Coryphaena hippurus) and rainbow runner (Elagatis bipinnulatus). Catch per unit of effort analysis shows that catch per unit of effort value of baby tuna (Thunnus spp.) increased on August 2004, whereas catch per unit of effort value of skipjack tuna (Katsuwonus pelamis) increased on September 2004. The length at first capture of skipjack tuna (Katsuwonus pelamis) was 49,3 FLcm. The catch size was bigger than the length at first maturity for skipjack tuna (Katsuwonus pelamis). The length at first capture of yellowfin tuna (Thunnus albacares) was 51,6 FLcm. This catch size was smaller than the length at first maturity for yellowfin tuna (Thunnus albacares).

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Responses of the red blood cells from two high-energy-demand teleosts, yellowfin tuna ( Thunnus albacares ) and skipjack tuna ( Katsuwonus pelamis ), to catecholamines

Journal of Comparative Physiology B ◽

10.1007/s003600050160 ◽

1998 ◽

Vol 168 (6) ◽

pp. 405-418 ◽

Cited By ~ 25

Author(s):

T. E. Lowe ◽

R. W. Brill ◽

K. L. Cousins

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Energy Demand ◽

High Energy ◽

Yellowfin Tuna ◽

Thunnus Albacares ◽

Skipjack Tuna ◽

Katsuwonus Pelamis

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Oxygen transport and cardiovascular responses in skipjack tuna (Katsuwonus pelamis) and yellowfin tuna (Thunnus albacares) exposed to acute hypoxia

Journal of Comparative Physiology B ◽

10.1007/bf00398338 ◽

1992 ◽

Vol 162 (2) ◽

pp. 131-143 ◽

Cited By ~ 75

Author(s):

Peter G. Bushnell ◽

Richard W. Brill

Keyword(s):

Oxygen Transport ◽

Acute Hypoxia ◽

Cardiovascular Responses ◽

Yellowfin Tuna ◽

Thunnus Albacares ◽

Skipjack Tuna ◽

Katsuwonus Pelamis

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Identification of yellowfin tuna (Thunnus albacares), mackerel tuna (Euthynnus affinis), and skipjack tuna (Katsuwonus pelamis) using deep learning

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/944/1/012009 ◽

2021 ◽

Vol 944 (1) ◽

pp. 012009

Author(s):

I Ayuningtias ◽

I Jaya ◽

M Iqbal

Keyword(s):

Deep Learning ◽

High Accuracy ◽

Yellowfin Tuna ◽

Thunnus Albacares ◽

Skipjack Tuna ◽

Learning Methods ◽

Katsuwonus Pelamis ◽

Economic Values ◽

Euthynnus Affinis

Abstract Yellowfin tuna (Thunnus albacares), mackerel tuna (Euthynnus affinis), and skipjack tuna (Katsuwonus pelamis) have important economic values for the capture fisheries in Indonesia. Activities of identifying these fish and other types of tuna have been done manually, which can lead to errors and ultimately affect statistics, stock estimates, or traceability. The aim of this research is to use deep learning methods in identifying three species of tuna, specifically yellowfin tuna, mackerel tuna, and skipjack tuna. YOLO’s newest model, YOLOv5, was used to identify the fish. The number of epochs that produces the optimum accuracy value for use in the YOLOv5 model is 400. The values for training loss, accuracy, precision, recall and F1-Score when the model is learning with a total of 400 epochs are 0.000253, 95%, 98.1%, 93.9%, and 96%. Based on these results, the three species of tuna can be identified with high accuracy.

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Cholinergic and adrenergic regulation of heart rate and ventral aortic pressure in two species of tropical tunas, Katsuwonus pelamis and Thunnus albacares

Canadian Journal of Zoology ◽

10.1139/z95-200 ◽

1995 ◽

Vol 73 (9) ◽

pp. 1681-1688 ◽

Cited By ~ 16

Author(s):

John E. Keen ◽

Richard W. Brill ◽

Sumi Aota ◽

Anthony P. Farrell ◽

David J. Randall

Keyword(s):

Heart Rate ◽

Aortic Pressure ◽

Yellowfin Tuna ◽

Thunnus Albacares ◽

Skipjack Tuna ◽

Adrenergic Stimulation ◽

Small Magnitude ◽

Katsuwonus Pelamis ◽

Adrenergic Regulation ◽

Adrenergic Blocker

Tonic cholinergic and adrenergic control of heart rate and ventral aorta pressure was examined in two species of tropical tunas, the skipjack tuna (Katsuwonus pelamis) and the yellowfin tuna (Thunnus albacares). Unlike that of many other teleosts, the basal heart rate in spinally blocked tunas (at 25 °C) was dominated by a cholinergic rather than an adrenergic tonus. Infusion of atropine increased the heart rate by 143 and 58% in skipjack and yellowfin tunas, respectively. Ventral aortic pulse pressure was significantly decreased and mean ventral aortic pressure was slightly increased. Blockade of β-adrenergic receptors with propranolol produced small (<6%) decreases in both heart rate and mean ventral aortic pressure, indicating a low level of tonic β-adrenergic stimulation. The small magnitude of the drop, however, suggests that tonic adrenergic regulation of heart rate and pressure is of less importance in tunas than in other teleosts, despite comparable circulating levels of adrenaline and noradrenaline. The α-adrenergic blocker phentolamine did not affect either heart rate or pressure. The intrinsic heart rate (i.e., the heart rate in the absence of cholinergic or adrenergic stimulation) was 180 beats/min in skipjack tuna and 119 beats/min in yellowfin tuna; these are the highest reported values for any teleost to date.

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Fishing season of large pelagic fish in Idi Rayeuk waters, East Aceh, Indonesia

DEPIK ◽

10.13170/depik.10.2.21203 ◽

2021 ◽

Vol 10 (2) ◽

pp. 167-173

Author(s):

M.A. Chaliluddin ◽

Rizka Alfita ◽

Thaib Rizwan ◽

Rahmat Rizqi ◽

Rosi Rahayu ◽

...

Keyword(s):

Time Series ◽

Water Surface ◽

Pelagic Fish ◽

Yellowfin Tuna ◽

Thunnus Albacares ◽

Skipjack Tuna ◽

Katsuwonus Pelamis ◽

Fishing Season ◽

Fish Samples ◽

Euthynnus Affinis

Large pelagic fish live on the water surface in groups. Skipjack tuna (Katsuwonus pelamis), yellowfin tuna (Thunnus albacares), mackerel tuna (Euthynnus affinis), mackerel (Scomberomous guttatus) are species of fishes that mainly catches by fishermen using purse seines. This study aims to determine the length and weight of large pelagic fish in the water of Idi Rayeuk and best the fishing season. This research was conducted in one month. Fish samples were collected from fishermen that used purse seine and landed their catches at the Idi Rayeuk Fishing Port, East Aceh. Additionally, the data was derived from the fishing port time-series during 2015 to 2019. The result showed that the yellowfin tuna (Thunnus albacares) has a length between 37 - 58 cm with a weight of 1.0 - 3.2 kg, skipjack tuna (Katsuwonus pelamis) 32 - 58 cm in length and weight 0.5 - 3.0 kg, mackerel tuna has 33 - 54 cm of length with a weight of 0.6 - 2.5 kg, and mackerel has 44 - 66 cm of length and weighing 0.7 - 1.7 kg. Its also found that the peak season for catching yellowfin tuna (Thunnus albacares) and mackerel tuna is May and June, and the lowest season in December for the yellowfin tuna, and December and January for the mackerel tuna. The peak season for skipjack fishing occurs in May, August, and October, and the low season occurs in November and December. Meanwhile, the peak season for fishing mackerel occurs in May, September, and October, while the low season occurs in January, February, and March.Keywords:Fishing seasonLarge pelagicPurse seineIdi RayeukAceh

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Length-Weight Relationships of Yellowfin Tuna Thunnus Albacares, Skipjack Tuna Katsuwonus Pelamis, Yaito Tuna Euthynnus Yaito, and Blue Round Scad Decapterus Maruadsi from Mischief Reef, South China Sea

International Journal of Innovative Studies in Aquatic Biology and Fisheries ◽

10.20431/2454-7670.0204005 ◽

2016 ◽

Vol 2 (4) ◽

Keyword(s):

South China Sea ◽

South China ◽

Yellowfin Tuna ◽

Thunnus Albacares ◽

Skipjack Tuna ◽

Katsuwonus Pelamis ◽

China Sea ◽

Decapterus Maruadsi

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A quantitative comparison of two kits for DNA extraction from canned tuna

Acta Veterinaria Brno ◽

10.2754/avb201988030315 ◽

2019 ◽

Vol 88 (3) ◽

pp. 315-322

Author(s):

Eliška Servusová ◽

Vladimír Babák ◽

Zora Piskatá ◽

Pavel Krčmář

Keyword(s):

Real Time ◽

Dna Extraction ◽

Species Identification ◽

Real Time Pcr ◽

Dna Analysis ◽

Extraction Methods ◽

Thunnus Albacares ◽

Species Determination ◽

Katsuwonus Pelamis ◽

Canned Tuna

The most common methods that can be used for species identification of tuna include methods based on detection of species-specific DNA via the polymerase chain reaction (PCR) method. The problem with DNA detection in processed products is the possibility of DNA fragmentation during the technological process. The quantity and quality of extracted DNA is a crucial step for species identification based on the DNA analysis. In this study, two DNA extraction methods (DNeasy Blood & Tissue Kit and DNeasy mericon Food Kit) for tuna DNA isolation were compared. Eight food products of canned tuna (three of them were declared as Thunnus albacares and five products were declared as Katsuwonus pelamis) with a different addition of various ingredients were tested. Furthermore, three different times of proteolysis (30 min, 60 min, overnight) for each sample and each extraction kit were evaluated. The DNA concentration was determined by a Qubit dsDNA HS Assay Kit fluorescence method and quantified using a Qubit fluorometer. The DNA purity was evaluated using the A260/A280 ratio of absorbances measured on a spectrophotometer. The main indicator of DNA quality and quantity was its amplifiability in the subsequent real-time PCR for Thunnus species, Thunnus albacares and Katsuwonus pelamis. Based on the results, both kits can be used for tuna species determination in highly heat-treated products with different composition, nevertheless, the DNeasy mericon Food Kit provided better statistical values in some parameters. The effect of different times of proteolysis was significant in most of the samples with regard to the crossing point values determined by real-time PCR.

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