scholarly journals Immunogenetic analysis of brown Schwyz cattle lines

2020 ◽  
pp. 55-60
Author(s):  
Mikhail Alekseevich Gontov ◽  
Svetlana Alekseevna Rusanova ◽  
Dmitriy Nikolaevich Koltsov ◽  
Valentina Ivanovna Dmitrieva ◽  
Vladimir Aleksandrovich Onufriev
Keyword(s):  
Genetic Structure ◽  
Milk Production ◽  
Genetic Similarity ◽  
Blood Groups ◽  
Interlinear Differences ◽  
Marker Alleles ◽  
Immunogenetic Analysis

Animals of brown Schwyz breed from pedigree farms of the Smolensk region were differentiated by their belonging to lines and related groups. Identification by blood groups was carried out according to the generally accepted technique, and the genetic structure of the uterine population of each line was established using marker alleles of the EAB locus; 12 genealogical lines were identified. The number of alleles in individual lines ranged from 15 to 53, which indicates the necessary hereditary diversity for improving the breed. At the same time, the most common B2O3Y2A`2E`2G`P`Q`Y`, I1Y2E`2G`I`G``1, G3O1T1Y2E`3F`2G``, G1O` and b markers somewhat offset the differences between them. The coefficient of genetic similarity r between lines and related groups of animals ranges from 0.20 to 0.90. Interlinear differences in milk production of cows were established.

CONSEQUENCES OF “HOLSTEINIZATION” OF UKRAINIAN BLACK-PIED DAIRY BREED BY GENE BOLA-DRB3.2

2017 ◽  
Vol 54 ◽  
pp. 146-156
Author(s):  
T. M. Suprovych ◽  
M. P. Suprovych ◽  
R. V. Kolinchuk
Keyword(s):  
Genetic Distance ◽  
Milk Production ◽  
Genetic Similarity ◽  
Restriction Analysis ◽  
Allelic Polymorphism ◽  
Milk Productivity ◽  
High Productivity ◽  
Black And White ◽  
Holstein Breed ◽  
Specific Pcr

Introduction. The main direction of increasing the productivity of milk is to increase the proportion of heredity of the Holstein breed in the genotype of cows. Industrial breeds in Ukraine are improving due to the increase in the Holstein inheritance in the genotype of cows. The "holsteinization" of the most widespread domestic Black-and-White diary breed is intensively conducted. Currently, the percentage of heredity from Holstein is 90% or more. The negative effect of "holsteinization" appeared in reducing the resistance of animals to diseases that led to the spread of necrobacterial pathology. The control of the spread of necrobacteriosis can be based on genetic markers. Important markers can be the allele of the BoLA-DRB3.2 gene responsible for the formation of adaptive immunity. Due to the ambiguity of the results of "holsteinization", the following tasks were solved: To study the genetic structure of the herd for the BoLA-DRB3.2 gene at the beginning of the "holsteinization" and now. To compare the detected genetic structures with the alleles spectrum of North American Holstein and identify quantitative and qualitative changes in the structure of the herd genotype. To determine the effect of "holsteinization" on the dynamics of milk production and the state of morbidity by necrobacteriosis. Materials and methods of research. Comparison of alleles of population of the Ukrainian Black-Pied Dairy (UBPD) breed and Holstein breed was conducted to detect the consequences of "holsteinization" on milk yield and incidence of necrobacteriosis. The data of the allelic polymorphism of the BoLA-DRB3.2 gene of the UBPD10 (2010, n = 162), UBPD15 (2015, n = 114) and two Holstein populations of the USA and Canada were collected. The allelic spectrum was determined by the PCR-RFLP method. The amplification of the BoLA-DRB3.2 gene was performed using 2-step PCR with the use of primers HLO-30, HLO-31 and HLO-32 and allele-specific PCR. Restriction analysis was performed with endonuclease RsaI, HaeIII, BstYI (XhoII). Restriction fragments were separated by electrophoresis in 4% agarose gel. Counting of allele frequencies was performed taking into account the number of homozygotes and heterozygotes found for the corresponding alleles. To determine the phylogenetic relationships between the populations of the studied herds, genetic distance and genetic similarity were determined by the M. Nei method. Individual dairy productivity of cows was estimated for all lactation (regardless of its duration). Average milk yields were determined as the total volume of milk produced divided by the number of dairy cows. Results and discussion. The breeding measures carried out led to the accumulation of alleles characteristic of the Holstein breed. For Holstein, there are eight alleles with a frequency of more than 4%. It is alleles *03, *07, *08, *11, *16, *22, *23, *24. A high degree of consolidation of weighty alleles can be outlined. In total they occupy 84,6% of allele spectrum of the population. Consolidation of such alleles in the herd of the Ukrainian Black-and-White diary breed is much lower - only 52.2%, although it increased by 6.2% over 5 years. Alleles *10, *13 and *28 are "weighty" for the Ukrainian Black-and-White diary breed, but they are almost non-existent in Holsteins. The genetic similarity of the herd UBPD15 and Holstein increased by ΔI = 0,085, and the genetic distance between the herds of the UBPD increased by ΔD = 0,085 for 5 years. The comparison of the allele spectrum of Holstein and the Ukrainian Black-and-White diary breed shows both the accumulation and the elimination of alleles associated with high productivity. The largest consolidation is typical for alleles *24 (+ 6.75%) and *16 (+ 4.65%). The frequency of "milk" alleles *22 and *08 decreased, respectively, by 4.14 and 1.27%. Alleys, which cause low milk productivity, have the following dynamics: * 23 + 2.53%, *11 – 0.67 and *28 – 0.26. The accumulation of alleles *16 and *23 (7.18%) was found that are associated with predisposition to necrobacteriosis and elimination of *03 and *22 alleles (4.75%) that influence on this disease. Conclusions. It is determined that the role of alleles characteristic for Holstein is increasing in the the Ukrainian Black-and-White diary herd. Breeding measures for holsteinization are conducted in the right direction. There is accumulation of alleles associated with high milk productivity and predisposition to necrobacteriosis. It positively affects the growth of milk production and negatively affects the incidence of necrobacteriosis.

scholarly journals Genetic variability in physic nuts cultivated in Northeastern Brazil

2013 ◽  
Vol 43 (6) ◽  
pp. 978-984 ◽  
Author(s):  
Vanice Dias Oliveira ◽  
Allivia Rouse Carregosa Rabbani ◽  
Ana Veruska Cruz da Silva ◽  
Ana da Silva Lédo
Keyword(s):  
Molecular Markers ◽  
Genetic Structure ◽  
Genetic Variability ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Dna Amplification ◽  
Northeastern Brazil ◽  
Jaccard Coefficient ◽  
Physic Nut ◽  
Genetic Structure And Diversity

This research had as objective to characterize genetically individuals of physic nut cultivated in experimental areas in Sergipe, Brazil by means of RAPD molecular markers. Leaves of 40 individuals were collected and DNA was isolated using CTAB 2% method. Were used 30 primers RAPD for DNA amplification, and this data was used to estimate the genetic similarity among the pairs of individuals, using Jaccard coefficient, and group them out for the UPGMA method. Also, the genetic structure and diversity of the populations were assessed using AMOVA. Of the 100 fragments generated, 29 of were polymorphic. A similarity average of 0.54 among the individuals was found and the amplitude similarities varied from 0.18 to 1.00. One of them (U5) was unit clusters and formed by the most divergent individuals. AMOVA indicated that there is more variation within (63%) the population. In conclusion, it was possible verify genetic variability in physic nut using RAPD markers at these experimental areas.

scholarly journals Determining the degree of kinship of sarlik yaks bred in the mountainous regions of the Kabardino-Balkarian Republic by blood groups

2021 ◽  
Vol 262 ◽  
pp. 02014
Author(s):  
Lyudmila Sabanchieva ◽  
Kristina Kanukova ◽  
Ismail Gazaev ◽  
Murat Gubzhokov ◽  
Zalina Bogotova
Keyword(s):  
Genetic Similarity ◽  
Structural Changes ◽  
Animal Husbandry ◽  
Genetic Distances ◽  
Blood Groups ◽  
Animal Breeding ◽  
Frequency Of Occurrence ◽  
Antigenic Composition ◽  
Mountainous Regions ◽  
Breeding Work

The formation of immunogenetics as a science was the result of numerous studies on the study of blood groups and other polymorphic systems, the results of which are successfully applied both in animal husbandry and in animal breeding. Modern biotechnological methods used to analyze blood groups enable us to determine the true origin of animals. To determine the correctness of the breeding processes occurring in the breed, the antigenic composition of blood groups, which are also used to study the reliability of origin, needs to be studied. The results obtained make it possible to analyze genetic similarity, determine the level of polymorphism and the nature of structural changes in the result of breeding work. Also, by analyzing the distribution of the frequency of occurrence of antigens, it is possible to determine the genetic distances and genetic similarity of breeds and lines among themselves [1].

scholarly journals FEATURES OF THE IMMUNOGENETIC STRUCTURE OF PIGS OF DOMESTIC BREEDS, SUITABLE FOR XENOTRANSPLANTATION

2020 ◽  
Vol 59 ◽  
pp. 105-114
Author(s):  
O. I. Metlytska ◽  
T. N. Ryk ◽  
V. I. Rossokha ◽  
A. A. Saenko
Keyword(s):  
Blood Group ◽  
Molecular Genetic ◽  
Statistical Processing ◽  
Molecular Genetic Analysis ◽  
Blood Groups ◽  
Marker Allele ◽  
Allele Distribution ◽  
Specialized Line ◽  
Selection Of ◽  
Immunogenetic Analysis

The aim of the work was to determine the immunogenetic characteristics of pigs of two Ukrainian breeds by their adaptability, resistance, reproduction, genetic homogeneity, the presence of alleles that determine the potential suitability for use in xenotransplantation and other biomedical purposes. At present, there is no breed or specialized line of pigs in Ukraine to address the urgent problems of humane medicine. However, there is a favorable situation for the creation of such a breed or the withdrawal of a specialized line because of the demand of pharmacological concerns for model biological objects to study the mechanisms of action of modern medical preparations, to develop methods of conducting bloodless surgical operations, modeling of the clinic and epidemiology of infectious diseases of different diseases. immune response, etc. In this context, there was a compelling reason to preserve the indigenous breeds of pigs of Ukraine, especially Ukrainian meat and Мyrgorod. Standard methods of immunogenetic analysis of pigs were used. The erythrocyte antigens of 9 blood group systems were determined using specific immune sera and the involvement of a bank of immunodiagnostics meeting international requirements. Blood groups were determined by the reaction of agglutination, an indirect Coombs test, and a hemolytic test. Statistical processing of the research results was performed using mathematical statistics using the GenAlex-6.0 computer program. As a result of the immunogenetic analysis of Ukrainian beef and myrrh-pig breeds, it was determined that each of them is characterized by a specific immunogenic profile, which is related to both breed characteristics and differences, and methods of their breeding. The difference between the distribution of the overwhelming number of alleles of blood groups was statistically significant. Pigs of Ukrainian meat and Мyrgorod breeds had the largest differences in allele distribution by B, E, F, K, L blood group systems with the presence of the Ladhjk marker allele in the latter (p < 0.05). The specificity of immunogenic profiles was evaluated, depending on the history of creation and the direction of the animal's performance. Species of Myrhorod and Ukrainian meat breeds with the presence of genotypes A - / - and Ebdgkmp / bdgkmp were found to determine the suitability of animals for xenotransplantation. Of the 80 animals tested for immunogenetic markers, only 24 individuals met the established selection criteria. The range of determined indicators of actual homozygosity for selected genotype animals ranged from 55.56% to the maximum value – 88.89%, mainly in sows from the Smorodinа, Rusalkа, Sorokа and Soyka families. According to molecular genetic analysis, you have been informed that the established criteria of the biomedical model meet only 13 individuals of Ukrainian meat breed. Among animals with the highest index of actual homozygosity, representatives of Cererа and Cіlina were identified, with Celina 4092 being homozygous for all blood group systems studied. The selection of pigs for blood alleles, desirable for xenotransplantation, with their transfer to the homozygous state: A -/- and Ebdgkmp / bdgkmp will impair the adaptive, first of all, reproductive qualities of the animals and create significant problems for the cultivation of such individuals. The possibility of the occurrence of reproductive and resistance disorders of pigs in the selection of blood groups genotypes modeled for xenotransplantation and the search for ways to overcome them are being considered.

scholarly journals IMMUNOGENETIC PARAMETERS OF THE RED-AND-WHITE COWS IN RELATION TO THE LINEAR AFFILIATION

2019 ◽  
pp. 32-39
Author(s):  
L. V. Efimova ◽  
E. V. Gatilova ◽  
O. V. Ivanova
Keyword(s):  
Comparative Analysis ◽  
Genetic Similarity ◽  
Main Part ◽  
Live Weight ◽  
Significant Incidence ◽  
Specific Antigens ◽  
High Degree ◽  
The Impact ◽  
Genotype V ◽  
Immunogenetic Analysis

The paper explores the urgency of increasing efficiency of breeding Red-and-White dairy cattle that take the main part of the population in the Krasnoyarsk region (69.7%), the immunogenetic characteristics of animals that belong to Montwick Chiftein and Pabst Hoverner (n=52) lines. The research on the blood groups of cows were conducted in the laboratory of immunogenetic analysis of Krasnoyarskagroplem. The authors revealed the differences in the incidence of specific antigens in the cows of two lines. The article found out that antigens A’2, B2, E’3, O3, and T2 (P>0.95-0.99) were found to be more frequent in Montwick Chiftein line cows, and antigen P2 (P>0.95) wasfound in Pabst Governer line cows. It was also revealed that the most rare antigens in the Montwick Chifthein line are antigens A’, A’1, O2, O4, Y1, R1, X1, M and E’2, and antigens B’’, O4, U and U’’in the Pabst Governer line. Interestingly, the authors identified the antigens that were not present in the animals of both lines and in each line separately. The average index of antigen incidence in the cows of each line showed a relatively low value. Due to this the authors made a conclusion about a high degree of heterozygosity of the studied individuals. The index of genetic similarity among the animals of two lines demonstrates a high value of 0.863. The polymorphism of the antigens in the EAF-V locus was studied and a significant incidence predominance in cows of the F allele over the V allele was found out. The impact of genotype according to the EAF-V locus on dairy productivity and live weight of the cows was determined: in the Montwick Chifthein line, cows with the FF genotype (5986,60172,41 kg) had a higher yield, in the Pabst Governer line - with the FV genotype (5914,20208,19 kg). Animals of both lines that had genotype V/V had higher live weight parmeters than animals with genotypes F/V and F/F. The comparative analysis of the obtained results was carried out with the results of other Russian authors who conducted similar studies.

Phylogeography and genetic structure of the slow worms Anguis cephallonica and Anguis graeca (Squamata: Anguidae) from the southern Balkan Peninsula

2014 ◽  
Vol 35 (2) ◽  
pp. 263-269 ◽  
Author(s):  
Evanthia Thanou ◽  
Sinos Giokas ◽  
Panagiotis Kornilios
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Structure ◽  
Genetic Similarity ◽  
Morphological Study ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Balkan Peninsula ◽  
Intraspecific Genetic Diversity ◽  
Well Differentiated

Two slow worm species are distributed at the southernmost part of the Balkan Peninsula: Anguis cephallonica, an endemic of the Peloponnese and the islands Zakynthos, Ithaki and Kephallonia, and A. graeca. Here, we investigate the intraspecific genetic diversity of A. cephallonica from the Peloponnese and Kephallonia and analyse A. graeca, from the northern Peloponnese, where it is found in sympatry with A. cephallonica. MtDNA and nDNA phylogenetic analyses confirm the genetic similarity of Peloponnesian and Kephallonian populations of A. cephallonica and reveal significant mtDNA genetic variation within it, probably related to the occurrence of multiple subrefugia in the Peloponnese. Peloponnesian A. graeca populations are genetically similar to non-Peloponnesian conspecifics implying recent dispersal to the Peloponnese. In contrast to the genetic markers, morphological characteristics (such as the number of mid-body scale-rows) failed to distinguish between Peloponnesian A. cephallonica and A. graeca. Although the former species is believed to be well-differentiated from its congeneric taxa, a thorough morphological study is needed.

Marker-assisted selection of candidate bulls for progeny testing programmes

1990 ◽  
Vol 51 (1) ◽  
pp. 63-74 ◽  
Author(s):  
Y. Kashi ◽  
E. Hallerman ◽  
M. Soller
Keyword(s):  
Theoretical Analysis ◽  
Genetic Markers ◽  
Milk Production ◽  
Marker Assisted Selection ◽  
Specific Marker ◽  
Progeny Testing ◽  
Genetic Progress ◽  
Potential Benefits ◽  
Marker Alleles ◽  
Selection Of

ABSTRACTA theoretical analysis of the potential benefits of marker-assisted selection (MAS) of candidate bulls prior to entry into a young sire progeny testing programme was carried out. It is assumed that quantitative trait loci (QTL) affecting milk production have been mapped with respect to known genetic markers, and MAS is based on evaluation of elite sires in order to identify marker alleles in coupling to favourable or unfavourable QTL alleles. Candidate bulls, descendants of the elite sire will then be selected, prior to conventional progeny testing, on the basis of the marker alleles derived from the elite-sire ancestor.The analysis considers recombination between marker and QTL, the difficulty of tracing specific marker alleles from sire to progeny, and the expectation that MAS, in practice, will be implemented in the grandsons, rather than in the sons of elite sires. It is shown that MAS of candidate bulls, based on the use of a single diallelic marker in linkage to a QTL will have only a negligible effect on the rate of genetic progress. Increases of 15 to 20% in the rate of genetic gain, however, can be obtained by the use of single polyallelic markers, and increases of 20 to 30% can be obtained by utilizing haplotypes of diallelic or polyallelic markers.

Spatial genetic structure of lowbush blueberry, Vaccinium angustifolium, in four fields in Maine

Botany ◽  
2009 ◽  
Vol 87 (10) ◽  
pp. 932-946 ◽  
Author(s):  
Daniel J. Bell ◽  
Lisa J. Rowland ◽  
Dapeng Zhang ◽  
Frank A. Drummond
Keyword(s):  
Genetic Structure ◽  
Genetic Similarity ◽  
Seedling Recruitment ◽  
Expressed Sequence Tag ◽  
Spatial Genetic Structure ◽  
Spatial Scales ◽  
Principal Component ◽  
Physical Distance ◽  
Vaccinium Angustifolium ◽  
Lowbush Blueberry

Expressed sequence tag – polymerase chain reaction (EST-PCR) molecular markers were used to infer spatial genetic structure of four lowbush blueberry ( Vaccinium angustifolium Ait.) fields in Maine. Genetic structure was quantified at three spatial scales: (1) within apparent clones (intrapatch), (2) among clones within a field, and (3) among fields separated by as much as 65 km. Of five “clones” or putative individuals examined in the intrapatch study, two showed complete genetic homogeneity within the patch, while three showed some band differences at their edges compared with their interiors. These differences at the edges, however, matched adjacent clones (so-called “intruders”), from which it was concluded that lowbush blueberry exhibits a fairly tight, phalanx clonal architecture with no evidence of invasive seedling establishment within clones. No significant correlation between genetic and physical distance was found among clones within fields via several statistical approaches. Significant among-field genetic differentiation was found via AMOVA (ΦPT = 8.4%; p ≤ 0.01) based upon transect samples across four fields ranging from 12.5 to 65 km apart. Principal component analysis and spatial autocorrelation (SA) corroborated these findings. Significant positive SA was found at the within-field distance class of <350 m, but SA decreased to an insignificant value by the first interfield distance of 12.5 km. A special form of SA analysis was employed to detect “hotspots” of genetic similarity between pairs of adjacent clones in two fields. Results indicated that 5 of 23 pairs of clones (21.7%) were genetically similar to each other, while the majority of pairs (18 of 23; 78.3%) showed random, decreasing patterns of genetic similarity. Results are discussed in terms of clonal dynamics including architecture, seedling recruitment, and inferred pollen or seed dispersal distances.

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