scholarly journals Petchoa in vitro culture

2020 ◽  
Author(s):  
T. A. Alatortseva
Keyword(s):  
In Vitro Culture ◽  
In Vitro Propagation ◽  
Optimal Concentration

The possibility of Petchoa hybrid Beautical Caramel Yellow in vitro propagation was investigated. The optimal concentration of phytohormones for regeneration has been established.

In vitro propagation of the bay laurel (Laurus nobilis.L) in Morocco

2014 ◽  
Vol 4 (3) ◽  
pp. 96-103
Author(s):  
Abdelali Chourfi ◽  
Tajelmolk Alaoui ◽  
Ghizlane Echchgadda
Keyword(s):  
Seed Germination ◽  
In Vitro Culture ◽  
In Vitro Propagation ◽  
Aerial Part ◽  
Basal Medium ◽  
Axillary Buds ◽  
Laurus Nobilis

Laurus nobilis L. is among the species which are most threatened by massive degradation in Morocco. The multiplication by seed or by cuttings gives very low percentages of recovery that is insufficient to meet the demand of growing market. In vitro culture proves to be a tremendous asset to solve this problem. Our work has focused on the study of seed germination of this species and its multiplication from microcuttings. Finally, we studied the ac-climatization ability of the plantlets resulting from this germination. The study of the germination, via the further measurement of the length of the aerial part and the roots and the number of axillary buds for nine weeks, showed that the MS basal medium was more efficient than media 1/2M.S and WPM. Among the eight tested hormones, IAA yielded the best growth of the plantlets. Hormonal combination of NAA and kinetin resulted into a per-centage of the greatest success in reaching 67 % micropropagation. The study also revealed that the MS basal medium in the presence of the IAA plants can acclimate most easily in two types of substrates with improved development in the peat alone.

scholarly journals Propagación In Vitro de Platicerium andinum Baker a partir de esporas

2018 ◽  
pp. 46-51
Keyword(s):  
Activated Carbon ◽  
Nicotinic Acid ◽  
In Vitro Culture ◽  
Sodium Hypochlorite ◽  
Forest Fires ◽  
In Vitro Propagation ◽  
Culture Media ◽  
Germination Rate ◽  
San Martín

Propagación In Vitro de Platicerium andinum Baker a partir de esporas In vitro propagation of Platicerium andinum Baker from spores Astriht Ruiz Rios1, Geyden Díaz Montes2 y Astrid Domy Gutiérrez Ruiz2 1Universidad Nacional de San Martín - Tarapoto, Jr. Maynas N° 177 - Tarapoto 2Corporación G y G E.I.R.L., Jr. 02 de Mayo N° 340 - Moyobamba DOI: https://doi.org/10.33017/RevECIPeru2015.0007/ Resumen Los bosques del departamento de San Martin, hábitat de Platycerium andinum B. viene siendo destruido de manera desmesurada, ocasionado por actividades antropogénicas, como la extracción de madera, incendios forestales, migración y cambio de uso de la tierra, lo que ha conducido a la especie a que actualmente se encuentre en peligro de extinción, sumándose a ello la extracción de la especie por su exuberante belleza para su comercialización como planta ornamental, asimismo a que sus esporas son difíciles de germinar en condiciones naturales. Además, no se cuenta con una metodología para la propagación in vitro de esta especie. La presente investigación tiene como objetivos determinar la concentración adecuada de hipoclorito de sodio para la obtención de esporas de Platycerium andinum B. libre de patógenos para su óptima germinación y evaluar tres medios de cultivo para determinar el medio más adecuado para la propagación de los gametofitos a través de cultivo in vitro. Las esporas fueron obtenidas de frondas fértiles de plantas adultas de Platicerium andinum B. haciendo un raspado de estas. Previa exposición de las esporas a una temperatura de 30 °C por espacio de 12 horas en estufa, estas fueron desinfectadas en una jeringa de 20 ml. en la cámara de flujo laminar con hipoclorito de sodio a tres diferentes concentraciones (T1: 0.5%, T2: 1% y T3: 1.5 %) por un tiempo de 20 minutos y cuatro enjuagues con agua destilada estéril; obteniendo como mejor resultando con el tratamiento T3: (1.5 %). La germinación de las esporas fue evaluada a partir de los 10 días, tiempo en el cual comenzaron a germinar y a los 30 días ya se tenía abundante tejido gametofitico; se evaluó a través del Índice de Germinación de las esporas (IG) utilizando la escala de abundancia-cobertura de Braun-Blanquet (Mermoz y Martín, 1993 modificada por Ramírez et al., 2000) llegando a los 60 días a la escala 5 (Cualquier número de gametofitos con cobertura mayor de 75%). En cuanto a la determinación del  mejor medio de cultivo para la propagación in vitro de gametofitos se trabajó con tres medios MSB (T1, T2 y T3) con aditivos de 0.4 ml. de thiamina, 0.5 de ácido nicotínico, 2 gramos de carbón activado y 20 gramos de sacarosa; con 100 ml de agua de coco en T2, y 200 ml en T3, obteniéndose como mejor resultado al tratamiento T1: (M y S Basal, con adición de 0.4 ml. de thiamina, 0.5 de ácido nicotínico, 2 gramos de carbón activado y 20 gramos de sacarosa). Descriptores: Gametofito, haploide, esporas, cultivo in vitro. Abstract Forests department of San Martin, habitat of Platycerium andinum B. is being destroyed disproportionately, caused by anthropogenic activities such as logging, forest fires, migration and changing land use, which has led to the species to which is currently in danger of extinction, adding to it the extraction of the species for its lush beauty for marketing as ornamental plant, also to the spores are difficult to germinate under natural conditions. Also, we do not have a methodology for in vitro propagation of the species. This research aims to determine the appropriate concentration of sodium hypochlorite to obtain spores of Platycerium andinum B., free of pathogens for optimum germination and evaluate three culture media to determine the most suitable medium for the propagation of the gametophytes through in vitro culture. The spores were obtained from fertile fronds of adult plants of Platicerium andinum B. making a scraping of these. Prior exposure of spores at a temperature of 30 °C for 12 hours in an oven, these were disinfected in a 20 ml syringe. In laminar flow chamber with sodium hypochlorite at three different concentrations (T1: 0.5%, T2: T3 1%: 1.5%) for a time of 20 minutes and four rinses with sterile distilled water; obtaining as being better with the treatment T3 (1.5%). The spore germination was evaluated after 10 days, at which time began to germinate and after 30 days we had plenty gametophytic tissue; it was evaluated through the germination rate of the spores (IG) using the scale of abundance-coverage Braun-Blanquet (Mermoz and Martin, 1993 as amended by Ramirez et al., 2000) coming to 60 days through 5 scale (Any number of gametophytes more coverage 75%). As for determining the best medium for the in vitro propagation of gametophytes we worked with three media MSB (T1, T2 and T3) with additives of 0.4 ml. thiamine, 0.5 nicotinic acid, 2 grams of activated carbon and 20 g of sucrose; with 100 ml of coconut water in T2, and 200 ml in T3, obtaining as best result for T1 (M and S Basal, added 0.4 ml thiamine, 0.5 nicotinic acid, 2 grams of activated carbon and 20 grams of sucrose). Keywords: Gametophyte, haploid spores, in vitro culture.

scholarly journals OPTIMASI NAA DAN BAP TERHADAP PERTUMBUHAN DAN PERKEMBANGAN TUNAS MIKRO TANAMAN KANTONG SEMAR (Nepenthes mirabilis) SECARA IN VITRO

2015 ◽  
Vol 5 (2) ◽  
pp. 29
Author(s):  
ROSMAINA ROSMAINA ◽  
DINNI ARYANI
Keyword(s):  
In Vitro Culture ◽  
Research Design ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Alternative Method ◽  
Shoot Number ◽  
Root Explant ◽  
Induction Stage

Conventional propagation of Nepenthes was difficult to do. To overcome the problems were required alternative method such as in vitro propagation. The objective of this research was to obtain the best treatment of BAP + NAA on shoot multiplication of Nepenthes through in vitro culture. The research design used Randomized Completely Design consist of seven treatments, e.g. 1) ½ MS0 (control); 2) ½ MS + 1 ppm BAP + 0.5 ppm NAA; 3) ½ MS + 1 ppm BAP + 1 ppm NAA; 4) ½ MS + 1.5 ppm BAP + 0.5 ppm NAA; 5) ½ MS + 1.5 ppm BAP + 1 ppm NAA; 6) ½ MS + 2 ppm BAP + 0.5 ppm NAA dan 7) ½ MS + 2 ppm BAP + 1 ppm NAA. The parameter observed were number of shoot, number of nodul, number of leafs, number of pitcher and number of root. The result of this research showed that treatment of ½ MS + 1 ppm BAP + 1 ppm NAA is the best treatment compared to others. At induction stage, this treatment can produce the number of shoot, number of nodul, and number of root were 1.6 shoots/explant, 10.8 nodul/explant and 3.6 root/explant, respectively. At subculture, this treatment can produce the number of shoot, number of leafs, and number of pitcher were 5.8 shoots/explant, 12.4 leafs/explant and 5.2 pitcher/explant, respectively.

scholarly journals A REVISED METHOD FOR SUCKER STERILIZATION TO SUPPORT THE IN VITRO PROPAGATION OF SAGO PALM (Metroxylon sagu Rottb.)

2014 ◽  
Vol 1 (1) ◽  
pp. 21
Author(s):  
Teuku Tajuddin ◽  
Karyanti . ◽  
Tati Sukarnih ◽  
Nadirman Haska
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
In Vitro Propagation ◽  
Large Scale ◽  
Culture Method ◽  
Large Area ◽  
Sago Palm ◽  
Superior Genotypes ◽  
Metroxylon Sagu

Hutan sagu (Metroxylon sagu Rottb.) dapat ditemukan dalam area yang cukup luas di wilayah Maluku dan Papua. Besarnya keanekaragaman hayati dari pohon sagu dapat dilihat di areal ini. Pohon sagu tumbuh secara alami terutama di daerah dataran atau rawa dengan sumber yang air melimpah. Tanaman sagu dapat diperbanyak dengan metode generatif melalui biji, dan vegetatif melalui tunas anakan. Dalam rangka mendukung perbanyakan pohon induk yang unggul secara in vitro dalam skala besar, perbaikan metode sterilisasi tunas anakan mutlak diperlukan. Tunas anakan muda (15-20 cm) yang diperoleh dari Propinsi Papua digunakan sebagai eksplan. Tujuan percobaan sterilisasi ini dilakukan untuk mendukung perbanyakan pohon sagu secara in vitro. Pada percobaan ini antibiotik digunakan untuk membersihkan jaringan internal eksplan dari jamur dan bakteri. Hasil percobaan ini menunjukkan bahwa campuran alkohol dan antibiotik dapat menekan pertumbuhan kontaminan.Kata kunci: Antibiotik, kontaminan jamur dan bakteri, kultur in vitro, metode sterilisasi, sagu ABSTRACTNatural sago (Metroxylon sagu Rottb.) forest can be found in large area in Maluku and Papua regions. There are wide genetic diversities of sago palm found in these areas. This palm grows along riverbanks and in swampy areas which are not suitable for other crops. Sago palm is propagated generatively by seed and vegetatively by suckers. With the purpose of establishing the in vitro culture method for a large-scale of mass clonally propagation of superior genotypes of sago palm, generating sterilized explants are very important. Young suckers (15-20 cm) obtained from areas of Papua Province were used as explants. The sterilization experiments were carrying out to support the tissue culture of sago palm. Sterilization was conducted using antibiotics in order to get rid of fungi and bacteria from inner part of explants tissues. The results showed that from all sterilization methods tested, the best result was treatment using alcohol and antibiotic as disinfectant agents.Keywords: Antibiotics, fungi and bacteria contaminants, in vitro culture, sterilization method, sago palm

scholarly journals In vitro culture of sempre-vivas species (Comanthera): a review

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Andressa Priscila Piancó Santos Lima ◽  
Alone Lima Brito ◽  
José Raniere Ferreira de Santana
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
In Vitro Propagation ◽  
Economic Importance ◽  
In Vitro Conservation ◽  
Future Research ◽  
In Vitro Cultivation ◽  
Natural Form

Abstract The term “sempre-viva” denotes plants whose structures retain their natural form and color after being cut and dried. For these reasons, they are commercially valuable for ornamental purposes. However, due to extractive overexploitation of their inflorescences, some of these species are considered endangered. The genus Comanthera includes the sempre-vivas species with greatest economic importance in Brazil. Previous studies have shown that tissue culture is a workable strategy for in vitro propagation and conservation of species of this genus. However, these studies are still incipient. Therefore, the objective of this review is to summarize the findings on the in vitro cultivation of species of the Comanthera genus, to serve as the basis for future research. The text is structured in two main topics: micropropagation and in vitro conservation.

scholarly journals EFFICIENT in vitro PROPAGATION OF Amaranthus viridis L. USING NODE EXPLANTS

2020 ◽  
Vol 19 (4) ◽  
pp. 41-51
Author(s):  
Tour Jan ◽  
Ikram Ullah ◽  
Bilal Muhammad ◽  
_ Tariq ◽  
Ali Mansoor ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Ammonium Nitrate ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Dark Green

Hyperhydricity is a frequently problem in plants during in vitro culture and affected micropropagation ofplants. To develop an efficient in vitro regenerated system without hyperdydricity, we demonstrated the effectof different disinfected agents (mercuric chlorite and hypochlorite), growth regulators, their concentrationsand combinations, Agar, pH, ammonium nitrate (NH4NO3) and number of subcultures. Mercuric chlorite at0.07% and exposing time (9–10 min) was appropriate for hygienic culture. The shoots induced by Benzyladnine(BA) alone or in combination with α-Naphthaleneacetic acid (NAA) exhibited maximum multiplicationwith symptoms of hyperhydricity than those induced by Kinetin alone or in combination with NAA. Hyperhydricitywas also reduced by increasing the concentration of agar, pH and elimination of NH4NO3 from themacroelements of Murashig and Skoog (MS) medium. Repeated subcultures affected both multiplication andhyperhydricity. The multiplication of shoots increased from parental culture up to 5th subculture and thereafterdeclined in 6th subculture. Although shoot hyperhydricity were observed from 1st subculture (19%) andthen increased up to 85% in 6th subculture. This increased in hyperhydricity could be due to the remaininginfluence of hormones. In shoots of 5th subculture the content of chlorophyll (dark green) were higher thanshoots of 6th subculture.

scholarly journals A Review of the in Vitro Propagation of Bauhinia Spp

2013 ◽  
Vol 21 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Jaime A. Teixeira Da Silva
Keyword(s):  
In Vitro Culture ◽  
In Vitro Propagation ◽  
Ornamental Plants ◽  
Biological Properties ◽  
Woody Ornamentals

Abstract Bauhinia species (including B. acuminata, B. variegata, B. purpurea, B. monandra, B. galpinii, B. blakeana and B. acuminata) are popular ornamental plants, usually woody ornamentals or herbaceous lianas, with attractive flowers typical of the Leguminosae of arid, temperate, sub-tropical and tropical zones. Bauhinia species also serve as fodder and many have multiple medicinal and biological properties. There is an interest in commerce and amongst collectors to clonally propagate species from this genus. This review highlights protocols that currently exist for the in vitro culture of Bauhinia species as a means to clonally propagate material.

scholarly journals Utility of in Vitro Propagation for Field-grown Broccoli: Effect of Genotype and Growing Season

HortScience ◽  
1993 ◽  
Vol 28 (6) ◽  
pp. 655-656 ◽  
Author(s):  
M.W. Farnham ◽  
B.V. Nelson
Keyword(s):  
Environmental Factors ◽  
In Vitro Culture ◽  
Brassica Oleracea ◽  
Shoot Regeneration ◽  
In Vitro Propagation ◽  
Culture Method ◽  
Growing Season ◽  
Genetic And Environmental Factors ◽  
Highly Correlated

We examined an in vitro culture method for propagating unconditioned, field-grown broccoli (Brassica oleracea L. Botrytis group) from peduncle explants by testing 20 cultivars in fall and spring. Propagation was affected significantly by genotype (cultivar) and season. The percentage of explants regenerating shoots was significantly higher for cultivars grown in spring (17% to 100%) than in fall (0% to 66%). Shoot regeneration from explants of plants within a cultivar also varied significantly (0% to 100%). Additionally, the number of propagules produced per explant was influenced by cultivar and was highly correlated with the percentage of explants regenerating shoots. This method for propagating field-grown broccoli lines is useful, but its applicability can be limited by genetic and environmental factors.

scholarly journals Efficient Regeneration of Hedychium coronarium through Protocorm-Like Bodies

Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1068
Author(s):  
Xiu Hu ◽  
Jiachuan Tan ◽  
Jianjun Chen ◽  
Yongquan Li ◽  
Jiaqi Huang
Keyword(s):  
In Vitro Culture ◽  
In Vitro Propagation ◽  
Adventitious Shoots ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Culture Methods ◽  
Hedychium Coronarium ◽  
Mature Node ◽  
First Time

Hedychium coronarium J. Koenig is a multipurpose plant with significant economic value, but it has been overexploited and listed as a vulnerable, near threatened or endangered species. In vitro culture methods have been used for propagating disease-free propagules for its conservation and production. However, explant contamination has been a bottleneck in in vitro propagation due to the use of rhizomes as the explant source. Plants in the family Zingiberaceae have pseudostems that support inflorescences, while rhizomes are considered true stems. The present study, for the first time, reported that the pseudostem bears nodes and vegetative buds and could actually be true stems. The evaluation of different sources of explants showed that mature node explants derived from the stem were the most suitable ones for in vitro culture because of the lowest contamination and the highest bud break rates. Culture of mature node explants on MS medium supplemented with 13.32, 17.76, and 22.20 μM 6-benzylaminopurine (BA), each in combination with 9.08 μM thidiazurin (TDZ) and 0.05 μM α-naphthaleneacetic acid (NAA) induced the conversion of buds to micro-rhizomes in six weeks. More than 96% of the micro-rhizomes cultured on MS medium supplemented with 17.76 μM BA, 6.81 μM TDZ, and 2.46 μM indole-3-butyric acid (IBA) were converted to globular-shaped clumps with protocorm-like bodies (PLBs). Further culture of a piece of the clumps induced more than 15 adventitious shoots. Adventitious roots were produced at the base of adventitious shoots, and plantlets were readily transplanted to a substrate for acclimatization in a shaded greenhouse. The survival rate of the plants in the greenhouse was up to 90%. Plants grew vigorously, and there were no off-types from the regenerated 11,100 plants. Our study also, for the first time, shows that H. coronarium can be regenerated via PLBs, which may represent a new way of the in vitro propagation of H. coronarium. The established protocol could be used for the increased propagation of H. coronarium for conservation or commercial production.

scholarly journals Chrysanthemum biotechnology: discoveries from the recent literature

2014 ◽  
Vol 26 (2) ◽  
pp. 67-77 ◽  
Author(s):  
Jaime A. Teixeira da Silva ◽  
Dariusz Kulus
Keyword(s):  
In Vitro Culture ◽  
In Vitro Propagation ◽  
Molecular Breeding ◽  
Recent Literature ◽  
Plant Science ◽  
Science Literature ◽  
Flowering Regulation ◽  
Made In

ABSTRACT The in vitro propagation of chrysanthemum (Chrysanthemum × grandiflorum (Ramat.) Kitam.), one of the world’s most important ornamentals, is a very well-studied topic and shows numerous strides each year. This mini-review condenses the knowledge that has been published on chrysanthemum biotechnology, especially in vitro culture in the wider plant science literature. In 2013 and 2014, important strides were made in molecular breeding, particularly anti-viral strategies, including through transgenics, and our understanding of flower genetics and flowering regulation.

Sign in / Sign up

Export Citation Format

Share Document