scholarly journals Optimum Medium for Lipase Production by Lipolytic Filamentous Fungi Isolated from Kendari Landfill Soil

2021 ◽  
Vol 38 (1) ◽  
Author(s):  
Miftahul Ilmi ◽  
Nur Rayani
Keyword(s):  
Aspergillus Niger ◽  
Olive Oil ◽  
Filamentous Fungi ◽  
Lipase Production ◽  
Esterification Reaction ◽  
Industrial Sectors ◽  
Lipase Enzyme ◽  
Rsm Optimization ◽  
Initial Medium ◽  
Significant Factors

Lipase produced by Aspergillus is widely known and used in many industrial sectors. Three lipolytic filamentous fungi had been isolated from Kendari (Southeast Sulawesi, Indonesia) landfill soil in previous study and identified as Aspergillus niger KE1, Aspergillus terreus KC1, and Aspergillus fumigatus KE6. However, the optimization of these isolate has not been reported. In this study, statistical optimization is selected because of more effective, efficient, economical, robust in giving the results and the possibility of analysing the interaction effects among factors. Three lipolytic isolates were screened in initial medium to obtain the highest lipolytic isolate and it was used in medium optimization process. Optimization was done using the series experimental design of Taguchi and RSM. Optimization was sucessfully obtain the optimum medium with the reduction of medium component from previously medium reported. Aspergillus niger KE1 is the selected isolate with the highest lipase productivity after 72 h in initial medium. The significant factors affected lipase production are peptone, olive oil, glucose and MgSO4.7H2O. The model equation obtained is Y = 1043 – 228 A + 300 B – 19803 C + 99 A*A + 5720 B*B + 292855 C*C – 979 A*B + 6563 A*C – 56338 B*C. This model predicts the lipase productivity succesfully with R2 of 96.9%. The optimized medium composes of peptone 2%, olive oil 0.1%, glucose 0.5% and MgSO4.7H2O 0.075%. Using the medium, the lipase productivity increases 4.7-folds compared with before optimization. Our results suggest that Aspergillus niger KE1 is a potential lipase source which catalyse the esterification reaction. Further research is needed to purify and characterize the lipase enzyme of this isolate.

scholarly journals Psychrotrophic Lipase Producers from Arctic Soil and Sediment Samples

2014 ◽  
Vol 63 (1) ◽  
pp. 75-82 ◽  
Author(s):  
R. RASOL ◽  
A.R. RASHIDAH ◽  
R. SITI NUR NAZUHA ◽  
J. SMYKLA ◽  
W.O. WAN MAZNAH ◽  
...  
Keyword(s):  
Olive Oil ◽  
Palm Oil ◽  
Colorimetric Method ◽  
Lipase Production ◽  
Northern Coast ◽  
Sediment Samples ◽  
Lipase Enzyme ◽  
Standard Plate ◽  
West Spitsbergen ◽  
Soil And Sediment

Culturable microorganisms were successfully isolated from soil and sediment samples collected in 2011 on the northern coast of Hornsund, West Spitsbergen. A total of 63 single colony isolates from three sampling sites obtained were subjected to temperature dependence study to assess whether they are obligate psychrophilic or psychrotrophic strains. From initial temperature screening, only 53 psychrotrophic isolates were selected that are capable of growing between 4-28 degrees C. The rest that were capable of tolerating higher temperatures up to 37 degrees C were not included in this study. These isolates were chosen for lipase enzyme screening confirmation with the standard plate assay of olive oil and fluorescent dye Rhodamine B. Six lipase positive isolates were also subjected for subsequent lipase enzyme plate screening on tributyrin, triolein, olive oil and palm oil agar. Lipase production by these six isolates was further assayed by using colorimetric method with palm oil and olive oil as the substrate. These isolates with promising lipase activity ranging from 20 U/ml up to 160 U/ml on palm oil and olive oil substrate were successfully identified. Molecular identification by using 16S rRNA revealed that five out of six isolates were Gram-negative Proteobacteria and the other one was a Gram-positive Actinobacteria.

scholarly journals Optimization of β-1,4-Endoxylanase Production by an Aspergillus niger Strain Growing on Wheat Straw and Application in Xylooligosaccharides Production

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2527
Author(s):  
Zahra Azzouz ◽  
Azzeddine Bettache ◽  
Nawel Boucherba ◽  
Alicia Prieto ◽  
Maria Jesus Martinez ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Wheat Straw ◽  
Plant Biomass ◽  
Residual Activity ◽  
Exchange Chromatography ◽  
Glycosyl Hydrolases ◽  
Biomass Degradation ◽  
Xylan Hydrolysis ◽  
Rsm Optimization ◽  
Single Carbon Source

Plant biomass constitutes the main source of renewable carbon on the planet. Its valorization has traditionally been focused on the use of cellulose, although hemicellulose is the second most abundant group of polysaccharides on Earth. The main enzymes involved in plant biomass degradation are glycosyl hydrolases, and filamentous fungi are good producers of these enzymes. In this study, a new strain of Aspergillus niger was used for hemicellulase production under solid-state fermentation using wheat straw as single-carbon source. Physicochemical parameters for the production of an endoxylanase were optimized by using a One-Factor-at-a-Time (OFAT) approach and response surface methodology (RSM). Maximum xylanase yield after RSM optimization was increased 3-fold, and 1.41- fold purification was achieved after ultrafiltration and ion-exchange chromatography, with about 6.2% yield. The highest activity of the purified xylanase was observed at 50 °C and pH 6. The enzyme displayed high thermal and pH stability, with more than 90% residual activity between pH 3.0–9.0 and between 30–40 °C, after 24 h of incubation, with half-lives of 30 min at 50 and 60 °C. The enzyme was mostly active against wheat arabinoxylan, and its kinetic parameters were analyzed (Km = 26.06 mg·mL−1 and Vmax = 5.647 U·mg−1). Wheat straw xylan hydrolysis with the purified β-1,4 endoxylanase showed that it was able to release xylooligosaccharides, making it suitable for different applications in food technology.

Effect of low shear modeled microgravity on phenotypic and central chitin metabolism in the filamentous fungi Aspergillus niger and Penicillium chrysogenum

2014 ◽  
Vol 106 (2) ◽  
pp. 197-209 ◽  
Author(s):  
Yesupatham Sathishkumar ◽  
Natarajan Velmurugan ◽  
Hyun Mi Lee ◽  
Kalyanaraman Rajagopal ◽  
Chan Ki Im ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Filamentous Fungi ◽  
Penicillium Chrysogenum ◽  
Modeled Microgravity ◽  
Low Shear

Utilisation of Jatropha press cake as substrate in biomass and lipase production from Aspergillus niger 65I6 and Rhizomucor miehei CBS 360.62

2017 ◽  
Vol 9 ◽  
pp. 103-107 ◽  
Author(s):  
M. Ilmi ◽  
C. Hidayat ◽  
P. Hastuti ◽  
H.J. Heeres ◽  
M.J.E.C. van der Maarel
Keyword(s):  
Aspergillus Niger ◽  
Press Cake ◽  
Rhizomucor Miehei ◽  
Lipase Production

scholarly journals OPTIMIZATION AND CHARACTERIZATION OF EXO-POLYGALACTURONASE BY Aspergillus niger CULTURED VIA SOLID STATE FERMENTATION

2018 ◽  
Vol 81 (1) ◽  
Author(s):  
Halifah Pagarra ◽  
Roshanida A. Rahman ◽  
Nur Izyan Wan Azelee ◽  
Rosli Md Illias
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Incubation Time ◽  
Industrial Applications ◽  
Screening Process ◽  
Industrial Sectors ◽  
Polygalacturonase Production

Polygalacturonases represent an important member of pectinases group of enzymes with immense industrial applications. The activity of exo-polygalacturonase produced by Aspergillus niger was studied in solid state fermentation (SSF) using Nephrolepis biserrata leaves as substrate. Central composite design (CCD) was used to optimize four significant variables resulted from the screening process that has been initially analyzed for the production of exo-polygalacturonase which are incubation time, temperature, concentration of pectin and moisture content. The optimum exo-polygalacturonase production obtained was 54.64 U/g at 120 hours of incubation time, temperature at 340C, 5.0 g/L of pectin concentration and 75.26% of moisture content. For partial characterization of exo-polygalacturonase, the optimum temperature and pH were obtained at 50°C and pH 4.0, respectively. SDS-PAGE analysis showed that molecular weight of exo-polygalacturonase were 35 and 71 kDa. This study has revealed a significant production of exo-polygalacturonase by A. niger under SSF using cheap and easily available substrate and thus could found immense potential application in industrial sectors and biotechnology

scholarly journals The AngFus3 Mitogen-Activated Protein Kinase Controls Hyphal Differentiation and Secondary Metabolism in Aspergillus niger

2015 ◽  
Vol 14 (6) ◽  
pp. 602-615 ◽  
Author(s):  
Bert-Ewald Priegnitz ◽  
Ulrike Brandt ◽  
Khomaizon A. K. Pahirulzaman ◽  
Jeroen S. Dickschat ◽  
André Fleißner
Keyword(s):  
Aspergillus Niger ◽  
Map Kinase ◽  
Secondary Metabolism ◽  
Filamentous Fungi ◽  
Cell Types ◽  
Growth Conditions ◽  
Mitogen Activated Protein Kinase ◽  
Content Type ◽  
Mitogen Activated Protein ◽  
Upstream Kinase

ABSTRACTAdaptation to a changing environment is essential for the survival and propagation of sessile organisms, such as plants or fungi. Filamentous fungi commonly respond to a worsening of their growth conditions by differentiation of asexually or sexually produced spores. The formation of these specialized cell types is, however, also triggered as part of the general life cycle by hyphal age or density. Spores typically serve for dispersal and, therefore, translocation but can also act as resting states to endure times of scarcity. Eukaryotic differentiation in response to environmental and self-derived signals is commonly mediated by three-tiered mitogen-activated protein (MAP) kinase signaling cascades. Here, we report that the MAP kinase Fus3 of the black moldAspergillus niger(AngFus3) and its upstream kinase AngSte7 control vegetative spore formation and secondary metabolism. Mutants lacking these kinases are defective in conidium induction in response to hyphal density but are fully competent in starvation-induced sporulation, indicating that conidiation inA. nigeris triggered by various independent signals. In addition, the mutants exhibit an altered profile of volatile metabolites and secrete dark pigments into the growth medium, suggesting a dysregulation of the secondary metabolism. By assigning the AngFus3 MAP kinase pathway to the transduction of a potentially self-derived trigger, this work contributes to the unraveling of the intricate signaling networks controlling fungal differentiation. Moreover, our data further support earlier observations that differentiation and secondary metabolism are tightly linked in filamentous fungi.

LIPASE ACTIVITIY OF MIXTURE OF FERMENTED AVOCADO (Persea americana), BANANA (Moses paradisiaca) AND SNAKEFRUIT (Salacca zalacca)

2017 ◽  
Vol 2 (1) ◽  
pp. 56
Author(s):  
Edy Agustian Yazid
Keyword(s):  
Enzyme Activity ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Olive Oil ◽  
Persea Americana ◽  
Musa Paradisiaca ◽  
Lipase Enzyme ◽  
Hydrolysis Process ◽  
Avocado Fruit ◽  
Oil Hydrolysis

<p><em>Fermentation is one of the bioconversion processes to produce anaerobic microbes which are beneficial and can produce enzymes. One of the enzymes that can be produced is lipase. Mixed fruit fermentation is an effort to produce lipase that can be done simply. The purpose of this research is to know the activity of lipase enzyme from mixture of fermented avocado fruit (Persea americana), bananas (Musa paradisiaca) and snakefruit (Salacca zalacca). Lipase enzyme activity is tested by measuring of free fatty acid (FFA) content obtained from olive oil hydrolysis process by lipase enzyme. The results showed the highest activity obtained 17.425 U/ml and the percentage of FFA produced was 12.94%. The highest activity of lipase enzyme was obtained at 45<sup>0</sup>C and pH 6.5. From this research can be concluded that the fermentation of the mixture of avocado, bananas and snakefruit can produce lipase enzyme that has relatively high activity.</em></p><p> </p><strong>Keywords: </strong><em>Fermentation, Lipase, Enzyme Activity, Fruits</em>

Functional characterization of Rho GTPases in Aspergillus niger uncovers conserved and diverged roles of Rho proteins within filamentous fungi

2011 ◽  
Vol 79 (5) ◽  
pp. 1151-1167 ◽  
Author(s):  
Min Jin Kwon ◽  
Mark Arentshorst ◽  
Eelke D. Roos ◽  
Cees A. M. J. J. van den Hondel ◽  
Vera Meyer ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Filamentous Fungi ◽  
Rho Gtpases ◽  
Functional Characterization ◽  
Rho Proteins

Recherche de nouvelles activités saccharifiantes thermostables chez les champignons filamenteux

1990 ◽  
Vol 36 (9) ◽  
pp. 625-630 ◽  
Author(s):  
P. Vanacker ◽  
B. Bacle ◽  
G. Vidal ◽  
L. Lacoste
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Key Words ◽  
Filamentous Fungi ◽  
Half Life ◽  
The Other ◽  
Thermostable Enzymes ◽  
Life Measure ◽  
Industrial Use ◽  
Better Than

We have searched for producers of a saccharifying activity with improved thermostability compared with industrial amyloglucosidases. These producers were chosen among thermophilic, thermotolerant, or even mesophilic fungi. Among the 846 isolated strains, five species (two Thermoascus spp., a member of the Aspergillus fumigatus group, and two members of the Aspergillus niger group) showed an amyloglucosidasic complex with the required property. Whereas the first three of these were thermophilic or thermotolerant strains, the latter two strengthen the idea that mesophilic strains can produce thermostable enzymes. The thermostability of the saccharifying complex of the Thermoascus spp., established with a half-life measure, was found to be far better than the other ones. The industrial use of these strains was discussed, and Thermoascus crustaceus seems to be the most advantageous one. Key words: filamentous fungi, amyloglucosidases, thermostability, Thermoascus.

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