scholarly journals Performance of Ceriporiopsis sp. in the Treatment of Black Liquor Wastewater

2016 ◽  
Vol 17 (2) ◽  
pp. 58 ◽  
Author(s):  
Ajeng Arum Sari
Keyword(s):  
Aquatic Ecosystems ◽  
Black Liquor ◽  
Oxygen Demand ◽  
White Rot Fungi ◽  
Dry Weight ◽  
Additional Treatment ◽  
White Rot ◽  
Empty Fruit Bunches ◽  
Potential Alternative ◽  
Different Strains

High amounts of black liquor wastewater are generated from bioethanol production by using oil palm empty fruit bunches. It contains an alkaline solution (NaOH), so it is quite toxic for aquatic ecosystems if discharged directly into waters. Black liquor has been treated by coagulation method, and it still needs additional treatment. This study aimed to determine degradation of black liquor wastewater by selected white-rot fungi (WRF). Five different strains of WRF have been tested for their ability to decolorize black liquor on agar and liquid media. Out of five fungi studied, two fungi, Ceriporiopsis sp. and Phanerochaete chrysosporium, showed the capacity to grow more than 50% on agar medium. In liquid medium, the percentage of decolorization of 15,000 ppm coagulated and diluted black liquor ranged from 70 to 89% at 30 days depending on the fungal strain. Ceriporiopsis sp. showed the better ability to decolorize black liquor than P. chrysosporium. The performance of Ceriporiopsis sp was evaluated regarding decolorization of black liquor, chemical oxygen demand (COD), and mycelial dry weight both in coagulated black liquor and original black liquor. The color of original and coagulated black liquor can be decolorized up to 90.13 and 86.85%, respectively. COD in original and coagulated black liquor was reduced up to 70.17 and 40.09%, respectively. The presence of coagulant Poly Aluminum Chloride (PAC) inhibited degradation of black liquor by fungus. The result demonstrated that Ceriporiopsis sp has a potential alternative to treat black liquor wastewater. 

A new method for evaluating decay: the effect of oxygen on Polyporuscompactus in red oak

1985 ◽  
Vol 15 (6) ◽  
pp. 1021-1024 ◽  
Author(s):  
T. J. Hall ◽  
Curt Leben
Keyword(s):  
Weight Loss ◽  
Continuous Flow ◽  
Constant Pressure ◽  
White Rot Fungi ◽  
Dry Weight ◽  
Ambient Air ◽  
White Rot ◽  
Weight Losses ◽  
Soil Block ◽  
Effect Of Oxygen

The effects of oxygen decay (weight loss) incited by Polyporuscompactus Overh. was studied using small fresh and dried wood blocks containing both sapwood and heartwood of similar radial position and age. A manifold was used to deliver hydrated gas mixtures of 1.0, 4.2, or 21% (ambient air) oxygen at constant pressure and continuous flow to jars containing inoculated or noninoculated blocks. Means (P = 0.05) for dry weight losses in inoculated blocks at 1.0, 4.2, and 21% oxygen were 3.3, 8.9, and 21.4%, respectively; losses may be comparable to decay by other white rot fungi using the soil-block procedure. Losses in noninoculated blocks at the above oxygen levels were 2.0, 4.6, and 7.0%, respectively. Differences in dry weight losses between fresh and dry blocks were found. In particular, losses in inoculated fresh blocks were significantly less than losses in inoculated dry blocks in ambient air. Native inhabitants occurring in fresh wood appear to interfere with the decay processes of P. compactus. Losses in noninoculated fresh blocks were significantly greater than in noninoculated dry blocks at 21% oxygen but not at 1.0 or 4.2% oxygen; losses were attributed to natural inhabitants in fresh wood.

scholarly journals Produksi dan karakterisasi lakase Omphalina sp. Production and characterization of Omphalina sp. laccase

2016 ◽  
Vol 75 (2) ◽  
Author(s):  
. SISWANTO ◽  
. SUHARYANTO ◽  
Rossy FITRIA
Keyword(s):  
Oil Palm ◽  
Laccase Activity ◽  
Research Result ◽  
White Rot Fungi ◽  
White Rot ◽  
Partial Purification ◽  
Empty Fruit Bunches ◽  
Optimum Ph ◽  
Laccase Enzyme

SummaryOmphalina sp. a white-rot fungi (WRF)originated from oil palm plantation has abilityto degrade empty fruit bunches of oil palm(EFBOP) so that it is expected to producelaccase with high activity. The ability ofOmphalina sp. to produce laccase enzyme onliquid fermentation will be studied. The enzymewill also be partially purified andcharacterized. The research result showed thatthe highest enzyme activity (1.162 U/mL) wasobtained using glucose malt yeast (GMY)medium at room temperature for four days.The addition of 2,5-xylidine as an inducerproduced laccase earlier i.e two days, but theactivity of laccase was less active afterprolonged incubation compared to that ofcontrol. The laccase produced on mediumcontaining 2% EFBOP reached optimumactivity as much as 0.38 U/mL after 10 th daysof incubation. Partial purification of laccaseon Sephacryl S-200 HR column resulted58.23% of yield recovery with twice purity thanbefore. The optimum pH of laccase was 4.5.Laccase activity was stable even after heatedon 50 o C for 30 minutes, but then decreasedwhen heated until 60 o C. The laccase has K Mand V max as much as 0.15 mM and 0.56 U/mLrespectively.RingkasanOmphalina sp., adalah fungi pelapuk putih(FPP) hasil isolasi dari kebun kelapa sawityang diketahui mampu mendegradasi tandankosong kelapa sawit (TKKS) dengan cepatsehingga diharapkan mampu menghasilkanlakase dengan aktivitas tinggi. KemampuanOmphalina sp. menghasilkan enzim lakasepada fermentasi cair akan dipelajari. Selain itu,lakase yang dihasilkan akan dimurnikan secaraparsial serta dilakukan karakterisasi pH, suhu,dan konsentrasi substrat optimum. Hasilpenelitian menunjukkan bahwa Omphalina sp.menghasilkan lakase dengan aktivitas tertinggi(1,162 U/mL) pada medium glucose malt yeast(GMY) yang diinkubasikan pada suhu ruangselama empat hari. Penambahan 2,5-xilidinsebagai induser mempercepat produksi lakaselebih awal yaitu dalam waktu dua hari, namunaktivitasnya masih lebih rendah dibandingkandengan kontrol pada inkubasi lebih lanjut.Lakase dari Omphalina sp. juga dapatdiproduksi pada medium yang mengandung2% TKKS dan aktivitasnya mencapai0,38 U/mL yang diinkubasi dalam suhu ruangselama 10 hari. Pemurnian parsial pada kolomSephacryl S-200 HR menghasilkan rendemensebesar 58,23% dengan kemurnian dua kalinya.Aktivitas lakase optimum pada pH 4,5 dantetap stabil setelah pemanasan selama 30 menitpada suhu ruang hingga 50 o C dan menuruntajam pada suhu 60 o C. Lakase Omphalina sp.menghasilkan nilai K M dan V maks masing-masing sebesar 0,15 mM dan 0,56 U/mL.

scholarly journals Pemanfaatan sistem rotary biological contactor menggunakan kultur Omphalina sp yang diimobilisasi pada tandan kosong kelapa sawit untuk absorbsi logam berat Cu2+ dan Hg2+ (Utilization of rotary biological contactor system using Omphalina sp immobilized on oil palm empty fruit bunch for absorption of heavy metals Cu2+ and Hg2+)

2017 ◽  
Vol 85 (2) ◽  
Author(s):  
Firda DIMAWARNITA ◽  
Tri PANJI
Keyword(s):  
Heavy Metals ◽  
Waste Treatment ◽  
Negative Impact ◽  
Enhanced Recovery ◽  
White Rot Fungi ◽  
Environmentally Friendly ◽  
Absorption Spectrometry ◽  
Treatment Method ◽  
White Rot ◽  
Empty Fruit Bunches

Heavy metals such as lead, chromium, cadmium, copper, mercury, and arsenic which are industrial by-products can have negative impact on the environment if they not managed properly. Biosorption is environmentally-friendly waste treatment method. The advantage of biosorption compared to other waste treatment method is ecotechnology, which means effective, cheaper than chemical treatment and environmentally-friendly technology. Using the concept of bioremediation-based on microbial enhanced recovery of metals (MERM) allows the possibility of concentration process (bio-concentration) of metal content through the process of biosorption in microbial cells. The process of bio-concentration potentially to be applied on a commercial scale to increase the recovery of high-value commercial metals. This study aims to absorb Cu2 + and Hg2 + metals using Omphalina sp. cultured on empty fruit bunches (EFB) in rotary biological contactor (RBC) systems. The principle of this technique is waste containing metals was contacted with white rot fungi (WRF) Omphalina sp. which is attached to the surface of the media inside a reactor and then the final waste concentration was analyzed. This analysis was using atomic absorption spectrometry (AAS) device to measure the concentration of Cu2+ and Hg2+ metal ions and Freundlich isothermic equations for calculating maximum absorption power (q max). The highest absorption of Cu2+ ion is 0.3304 mg/g and Hg2+ is 0.074 mg/g for 6 hours. The adsorption capacity of Omphalina sp. for Cu2 + and Hg2 + solutions based on the Freundlich adsorption equation with maximum adsorption power for Cu2 + is 71.911 mg/g and Hg2+ is 20.216 mg/g. Biosorbent Omphalina sp. can be reused in biosorption process with percentage decrease of Cu2+ is4.30% (4.3 ppm) and Hg2 + is 27.80% (1.39 ppm) for 18 hours.[Key words : biosorption technique, empty fruit bunches, immobilization, rotary biological contactor , white rot fungi-Omphalina sp.] AbstrakLogam berat seperti timbal, krom, cadmium, tembaga, merkuri, dan arsen yang merupakan hasil samping industri dapat berdampak negatif terhadap lingkungan jika tidak dikelola dengan baik. Biosorpsi merupakan metode pengolahan limbah yang ramah lingkungan.  Keunggulan biosorpsi dibandingkan dengan metode pengolahan limbah yang lainnya adalah ekoteknologi yaitu teknologi efektif, murah dan ramah lingkungan. Melalui konsep bioremediasi berbasis microbial enhanced recovery of metals (MERM) memungkinkan terjadinya proses pemekatan (bio-konsentrasi) kandungan logam melalui proses biosorpsi dalam sel mikroba. Proses bio-konsentrasi tersebut berpeluang untuk diterapkan dalam skala komersial untuk meningkatkan recovery logam bernilai komersial tinggi. Penelitian ini bertujuan untuk meng-absorpsi logam Cu2+ dan Hg2+ menggunakan kultur Omphalina sp. pada media TKKS dalam sistem rotary biological contactor (RBC). Prinsip teknik ini yaitu limbah yang mengandung logam dikontakkan dengan kultur JPP Omphalina sp. yang melekat pada permukaan media di dalam suatu reaktor kemudian konsentrasi limbah akhir dianalisis kandungannya. Analisis ini mengguna-kan alat spektrometri serapan atom (SSA) untuk mengukur konsentrasiion logam Cu2+ dan Hg2+ dan persamaan isoterm Freundlich untuk menghitung daya absorbsi maksimum (q max). Penyerapan tertinggi ion Cu2+ sebesar 0,330 mg/g dan Hg2+ sebesar 0,074 mg/g selama 6 jam. Kapasitas adsorpsi Omphalina sp. terhadap larutan Cu2+ dan Hg2+ berdasarkan persamaan adsorpsi Freundlich dengan daya adsorpsi maksimumnya untuk Cu2+sebesar 71,911 mg/g dan untuk Hg2+ sebesar 20,216 mg/g. Biosorben Omphalina sp. dapat digunakan ulang dalam proses biosorpsi dengan persentase penurunan Cu2+ sebesar 4,30% (4,3 ppm) dan Hg2+ sebesar 27,80% (1,39 ppm) dalam waktu 18 jam.[Kata kunci : imobilisasi, jamur pelapuk putih Omphalina sp, rotary biological contactor, teknik biosorpsi logam, tandan kosong kelapa sawit.]

scholarly journals Biosorpsi ion tembaga dalam limbah tailing menggunakan jamur pelapuk putih amobil Biosorption of copper ion in tailing mining effluent using immobilizede white-rot fungi

2016 ◽  
Vol 83 (1) ◽  
Author(s):  
Firda DIMAWARNITA ◽  
. SUHARYANTO ◽  
. TRI-PANJI1 ◽  
Nur RICHANA ◽  
Achmad ZAINUDIN
Keyword(s):  
Heavy Metals ◽  
Agricultural Land ◽  
Mining Industry ◽  
Copper Ion ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Absorption Capacity ◽  
White Rot ◽  
Pollution Level ◽  
Empty Fruit Bunches

Abstract         Copper and gold mining industry produce large amount of tailings effluent containing heavy metals such as Cu and Hg that will polute  environment and agricultural land, if it is not properly treated.  Effort in reducing pollution level and recovery of metal in tailing could be conducted with biosorption process using microbial cell as an absorbent agent. This study aims to determine the ability of selected white rot fungi (WRF) immobilized on empty fruit bunches of oil palm (EFB)  to absorb Cu (II) metals in tailing. Based on growth rate, ligninolytic enzymes activity, and the absorption capacity of heavy metals, the superior WRF candidate was Omphalina sp. The ability of biomass Omphalina sp. to decrease the con-centrateion of Cu (II) with an initial concentration of 150 ppm, up to 63%. Omphalina sp. that immobilized on TKKS was able to reduce Cu (II) as much as 66-77% at pH 4.0 for 60 minutes. Abstrak                Eksploitasi tambang tembaga dan emas banyak menghasilkan limbah tailing yang masih me-ngandung logam berat seperti Cu (II) dan Hg (II). Limbah tersebut berpotensi mencemari perairan dan lahan pertanian bila tidak ditangani dengan baik.  Usaha untuk mengatasi limbah tailing dan sekaligus memekatkan (recovery) logam di dalam-nya dapat dilakukan dengan proses biosorpsi menggunakan sel mikroba. Penelitian ini bertujuan menetapkan kemampuan biomassa jamur pelapuk putih (JPP) yang diimobilisasi pada tandan kosong kelapa sawit (TKKS) dalam mengabsorpsi ion logam Cu (II). Metode biosorpsi logam yang digunakan dengan sistem batch dengan kadar logam 300 ppm, 200 ppm,dan 150 ppm. Hasil seleksi JPP berdasarkan laju pertumbuhan, aktivitas enzim ligninolitik, dan penyerapan logam berat telah  diperoleh   kandidat   JPP   unggul  yaitu Omphalina sp. Dalam media PDB,  Omphalina sp. toleran terhadap Cu (II) hingga konsentrasi 150 ppm. Kemampuan biomassa Omphalina sp. untuk penurunan konsentrasi Cu (II) dengan konsentrasi awal 150 ppm yaitu mencapai 63%. Omphalina sp. yang diimobilisasi dengan TKKS mampu menurun- kan kandungan Cu (II) sebesar 66-77%  pada pH 4.0 selama 60 menit. 

Black Liquor Decolorization by Selected White-Rot Fungi

2011 ◽  
Vol 165 (2) ◽  
pp. 406-415 ◽  
Author(s):  
Verónica Da Re ◽  
Leandro Papinutti
Keyword(s):  
Black Liquor ◽  
White Rot Fungi ◽  
White Rot

Bioremediation of a wine distillery wastewater using white rot fungi and the subsequent production of laccase

2007 ◽  
Vol 56 (2) ◽  
pp. 179-186 ◽  
Author(s):  
P.J. Strong ◽  
J.E. Burgess
Keyword(s):  
Biological Treatment ◽  
Oxygen Demand ◽  
White Rot Fungi ◽  
White Rot ◽  
White Rot Fungus ◽  
Laccase Production ◽  
Total Phenolic ◽  
Pycnoporus Cinnabarinus ◽  
Distillery Wastewater

The aim of this work was to ascertain whether a submerged culture of a white rot fungus could be used to treat distillery wastewater, and whether the compounds present in the wastewater would stimulate laccase production. Trametes pubescens MB 89, Ceriporiopsissubvermispora, Pycnoporus cinnabarinus and UD4 were screened for their ability for the bioremediation of a raw, untreated distillery wastewater as well as distillery wastewater that had been pretreated by polyvinylpolypyrrolidone. Suitability of each strain was measured as a function of decreasing the chemical oxygen demand (COD) and total phenolic compounds concentration and the colour of the wastewater, while simultaneously producing laccase in high titres. After screening, T. pubescens MB 89 was used further in flask cultures and attained 79±1.1% COD removal, 80±4.6% total phenols removal, 71±1.6% decrease in colour at an absorbance of 500 nm and increased the pH from 5.3 to near-neutral. Laccase activity in flask cultures peaked at 4,644±228 units/l, while the activity in a 50 l bubble lift reactor peaked at 12,966±71 units/l. Trametes pubescens MB 89 greatly improved the quality of a wastewater known for toxicity towards biological treatment systems, while simultaneously producing an industrially relevant enzyme.

Étude comparée de la dégradation de lignocelluloses par différentes souches de Fomes annosus

1983 ◽  
Vol 29 (12) ◽  
pp. 1683-1688 ◽  
Author(s):  
J. J. Bono ◽  
G. Gas ◽  
A. M. Boudet ◽  
J. Fayret ◽  
C. Delatour
Keyword(s):  
Nitrogen Content ◽  
Lignin Degradation ◽  
White Rot Fungi ◽  
White Rot ◽  
Wood Degradation ◽  
Specific Behaviour ◽  
Sporotrichum Pulverulentum ◽  
Coniferous Wood ◽  
Different Strains ◽  
Low Nitrogen

The ligninolytic activity of Fames annosus has been estimated by radiorespirometric methods using lignocelluloses of Norway spruce and poplar, as substrates, labelled on the lignin moiety. Although F. annosus is mainly a parasite of coniferous wood, poplar lignins were more extensively degraded. 14C-labelled poplar lignocelluloses, labelled either on the lignins moiety or on the overall polymers, were used to compare wood degradation or only specific lignin degradation by the different strains. Moreover, using the best strain, we studied the effect of culture parameters which are known to influence lignin degradation by Sporotrichum pulverulentum. The rate of lignonolysis was enhanced in media with low nitrogen content but was not stimulated by increasing the O2 concentration of the atmosphere. Such data point out the specific behaviour of F. annosus, which differs in some respect from other white-rot fungi.

scholarly journals Bioremediation of Indigosol Blue 04B Batik Effluent by Indigenous Fungal Isolates, Aspergillus spp.

Omni-Akuatika ◽  
2018 ◽  
Vol 14 (2) ◽  
Author(s):  
Ratna Stia Dewi ◽  
Rina Sri Kasiamdari ◽  
Erni Martani ◽  
Yekti Asih Purwestri
Keyword(s):  
Culture Media ◽  
Electrical Conductance ◽  
Oxygen Demand ◽  
White Rot Fungi ◽  
Positive Control ◽  
White Rot ◽  
Fungal Treatment ◽  
Before And After ◽  
Standard Values ◽  
Aspergillus Sp

Effluent from the local batik home industry is a serious problem, because the effluent discharge generated is spread in different places. Untreated effluent can cause environmental pollution, such as in groundwater reservoirs,because most is discharged into rivers. The aim of this research was to evaluate the bioremediation potential of indigenous fungi in liquid culture media with Indigosol Blue 04B (IB) batik effluent. The fungi isolates tested were Aspergillus sp. 1, Aspergillus sp. 2 and Aspergillus sp. 3, isolated from dye effluent soil and batik effluent, and compared to white rot fungi (Phanerochaete chrysosporium) as a positive control.   The physiochemical properties of IB batik effluent before and after fungal treatment were investigated. All of these parameters before the fungal treatment were above the recommended standard values based on the Governor regulation of Yogyakarta Special Region No. 7/2010. The level of biochemical oxygen demand (BOD), chemical oxygen demand (COD), total dissolved solids (TDS), total suspended solids (TSS), and electrical conductance (EC) was reduce by Aspergillus spp. The highest percentage reduction was achieved by Aspergillus sp. 3, namely 88.34% BOD, 89.11% COD, 75.77% TSS, 85.85% TDS and 71.21% EC, after 3 days of incubation. These results show that the positive control isolate had the lowest value. The study confirms the ability of indigenous fungi isolates in the remediation of IB batik effluent and their potential for future analysis in the treatment of all types of batik effluent.

Effect of Culture Technique of Ganoderma Australe Mycelia on Percentage Removal of Leachate Organics

2016 ◽  
Vol 13 (1) ◽  
pp. 131
Author(s):  
Wan Razarinah Wan Abdul Razak ◽  
Noor Zalina Mahmood ◽  
Noorlidah Abdullah
Keyword(s):  
Landfill Leachate ◽  
Oxygen Demand ◽  
White Rot Fungi ◽  
Culture Technique ◽  
Biological Oxygen Demand ◽  
White Rot ◽  
Toxic Substances ◽  
Percentage Removal ◽  
Ganoderma Australe ◽  
Biological Methods

Leachate (liquid pollutant), which is highly contaminated with organic matter and toxic substances is a major problem that arised from landfill. Biological methods have proven to be effective to remove organic matters that are abundant in leachate. This study is intended to compare the used of free mycelia and immobilized mycelia of the white-rot fungi, Ganoderma australe for the  removal of landfill leachate organics. The organics fraction of landfill leachate was measured by biological oxygen demand (BOD5), and chemical oxygen demand (COD). The experiment revealed that free mycelia of G. australe showed capability in removing leachate BOD5  but not COD. However, the use of immobilized G. australe displayed the best result in the removal of BOD5 and COD leachate after 4 weeks of treatment in flasks with 93.09% and 17.84% percentage removal of BOD5 and COD, respectively. Therefore, G. australe can be considered potentially useful in the treatment of landfill leachate as they can help in removing BOD and COD due to their biodegradative abilities.

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