scholarly journals Efek Anti Diabetes Buah Pare (Momordica charantia Linn.) Terhadap Kadar Glukosa Darah, Sel Penyusun Pulau Langerhans dan Sel Leydig pada Tikus Putih Hiperglikemia

2017 ◽  
Vol 4 (2) ◽  
pp. 43-50
Author(s):  
I Dewa Putu Anom Adnyana ◽  
Dewa Ketut Meles ◽  
. Wurlina ◽  
Sunarni Zakaria ◽  
Niluh Suwasanti
Keyword(s):  
Rattus Norvegicus ◽  
Momordica Charantia

Penelitian ini bertujuan untuk membuktikan ekstrak buah pare (Momordica charantia Linn.) terhadap kadar glukosa darah, sel penyusun pulau Langerhans dan sel Leydig tikus putih (Rattus norvegicus) hiperglikemia. Tikus putih sebanyak 25 ekor dibagi secara acak menjadi 5 kelompok. Induksi aloksan dengan dosis 150 mg/kgbb secara intraperitoneal untuk menimbulkan kerusakan pankreas dilakukan pada 5 kelompok perlakuan. Tiga kelompok perlakuan diterapi dengan berbagai dosis ekstrak buah pare, (P1) 29 mg/1ml/hari, (P2) 50 mg/1ml/hari, dan (P3) 59 mg/1ml/hari, satu kelompok sebagai kontrol negatif (P0) diberi CMC Na 0,5% 1ml/hari, kontrol positif (K+) diberi Glibenclamide® 0,126 mg/1ml/hari. Ekstrak buah pare diberikan selama 21 hari. Kadar glukosa diperiksa setelah 2 jam, 4 jam, 6 jam dan 8 jam pascapemberian dihari pertama. Kadar glukosa selanjutnya diperiksa pada hari ke 7, 14 dan 21. Semua tikus dieuthanasia setelah 21 hari perlakuan, pankreas dan testis diambil untuk dibuat preparat histopatologi. Hasil menunjukkan bahwa ekstrak buah pare (Momordica charantia Linn.) memiliki efek antidiabetik yang dapat menurunkan kadar gula darah, meningkatkan jumlah sel insula Langerhans dan meningkatkan jumlah sel Leydig pada dosis 50 mg/1ml/hari pada hari ke 21 setelah perlakuan.

scholarly journals PENGARUH EKSTRAK DAUN PARE (Momordica charantia) TERHADAP FOLIKEL OVARIUM TIKUS (Rattus norvegicus) DALAM KONDISI HYPERGLYCEMIC

2020 ◽  
Vol 8 (1) ◽  
pp. 61
Author(s):  
Ganeswara Muharam Hazmi Rezady ◽  
Hani Plumeriastuti ◽  
Rimayanti Rimayanti
Keyword(s):  
Treatment Group ◽  
Alternative Treatment ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Momordica Charantia ◽  
Once Daily ◽  
Significant Difference

The purpose of this study was to prove that the provision of pare leaf extract can reduce the process of follicular atrophy in the ovaries caused by hyperglycemic conditions. The total of this study was completed within 20 days, including the induction of Streptozotocin for 5 days, treatment for 14 days, and 1 day for ovarian collection. The K- group as a negative control without being induced by Streptozotocin and received no treatment. The K+ group as a positive control was only induced by Streptozotocin at a dose of 55 mg / Kg BW and D10 without treatment. The T1 group was induced by Streptozotocin at doses of 55 mg / Kg BW and D10 and treated with pare leaves extract (Momordica charantia) at a dose of 1.26 ml / Kg BW. T2 group was induced by Streptozotocin with dose of 55 mg / Kg BW and D10 and was treated with pare leaves extract (Momordica charantia) at a dose of 2.52 ml / Kg BW. Induction of Streptozotocin intraperitoneally and once-daily peroral treatment. The result showed that treatment group could make a significant difference with the positive control. Showing that amount of gap could make Momordica charantia to be alternative treatment for hyperglycemic condition.

scholarly journals Perbandingan kualitas spermatozoa tikus wistar (rattus norvegicus) yang diberi ekstrak buah pare (momordica charantia L) dengan ekstrak biji pepaya (carica papaya L)

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Kyrie E. Wenur ◽  
Lusiana Satiawati ◽  
Lydia Tendean
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Carica Papaya ◽  
Seed Extract ◽  
Momordica Charantia ◽  
Bitter Melon ◽  
Fruit Extract ◽  
Treatment Groups ◽  
Papaya Seeds

Abstract: Male participation in contraception is still lacking. Pare (Momordica charantia L) is known having an inhibitory effect spermatogenesis and seeds of papaya (Carica papaya L) contain the alkaloid compounds that are anti-fertility, both can be used as a male contraceptive. The purpose of this study was to determine sperm quality comparison Wistar rats (Rattus norvegicus) were given a fruit extract of pare (Momordica charantia L) with the seed extract of papaya (Carica papaya L). The research used a completely randomized experimental design. There are nine samples of Wistar rats (Rattus norvegicus) males were divided into three groups and one group consisting three Wistar rats. Over the past 50 days, extracts of pare (Momordica charantia L) is given to the group 1 at 70mg / kg/day, while in the group 2 given seed extract of papaya (Carica papaya L) of 70mg / kg/day and the control group was given no treatment. The results showed significant differences in sperm motility among the treatment groups 1 with extract of bitter melon fruit (Momordica charantia L) and 2 treatment groups with the extract of papaya seeds (Carica papaya L), namely a decrease in sperm motility (p = 0.002). Conclusion, the fruit extract of bitter melon (Momordica charantia L) and seed extract of papaya (Carica papaya L) affects the quality of spermatozoa.Keywords: pare, papaya seeds, quality of spermatozoa. Abstrak: Partisipasi pria dalam menggunakan alat kontrasepsi masih sangat kurang. Pare (Momordica charantia L) diketahui memiliki efek penghambatan spermatogenesis dan biji pepaya (Carica papaya L) mengandung senyawa alkaloid yang bersifat antifertilitas, keduanya dapat digunakan sebagai bahan kontrasepsi pria. Tujuan penelitian ini adalah mengetahui perbandingan kualitas spermatozoa tikus wistar (Rattus norvegicus) yang diberi ekstrak buah pare (Momordica charantia L) dengan ekstrak biji pepaya (Carica papaya L). Penelitian ini menggunakan metode penelitian eksperimental dengan menggunakan rancangan acak lengkap. Sampel yang digunakan adalah sembilan tikus wistar (Rattus norvegicus) jantan yang dibagi menjadi tiga kelompok dan satu kelompok terdiri dari tiga ekor tikus wistar. Selama 50 hari, ekstrak buah pare (Momordica charantia L) diberikan pada kelompok perlakuan 1 sebesar 70mg/kgBB per hari, sedangkan pada kelompok perlakuan 2 diberikan ekstrak biji pepaya (Carica papaya L) sebesar 70mg/kgBB per dan kelompok kontrol tidak diberikan perlakuan. Hasil penelitian menunjukan adanya perbedaan signifikan pada motilitas spermatozoa antara kelompok perlakuan 1 dengan pemberian ekstrak buah pare (Momordica charantia L) dan kelompok perlakuan 2 dengan pemberian ekstrak biji pepaya (Carica papaya L) yaitu penurunan motilitas spermatozoa ( p = 0,002).Kesimpulan, ekstrak buah pare (Momordica charantia L) dan ekstrak biji pepaya (Carica papaya L) berpengaruh terhadap kualitas spermatozoa. Kata kunci: buah pare, biji pepaya, kualitas spermatozoa

scholarly journals Pemberian Ekstrak Buah Pare dan Diet Tinggi Lemak pada Penurunan Berat Badan Tikus Putih Jantan

2019 ◽  
Vol 17 (1) ◽  
pp. 60
Author(s):  
I MADE SUBHAWA HARSA
Keyword(s):  
Rattus Norvegicus ◽  
High Fat Diet ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Momordica Charantia ◽  
Control Group ◽  
Bitter Melon ◽  
High Fat ◽  
White Rats

<div class="Section1"><p align="center"><strong>Abstra</strong><strong>ct</strong></p><p><strong>Background</strong> : Obesity is an energy balance disorder, ketogenic diet can reduce body weight. Bitter melon (<em>Momordica charantia L.)</em> is a type of vegetable material that is often used as an antioxidant, hypocholesterolemia and hypotriglyceridemia drug. <em>Cucurbitasin</em> is a substance produced by bitter melon (<em>Momordica charantia L.)</em> with a bitter taste which causes in decreasing appetite, resulting in a decrease in triglycerides. This is caused by the bioactivity of bitter melon (<em>Momordica charantia L.)</em> which can increase lipid oxidation, which causes the accumulation of decreased fat tissue which can reduce weight.</p><p><strong>Methods</strong>: This research using pre and post test randomized controlled group design with sample size of 30 male white rats (<em>Rattus norvegicus</em>) wistar lines divided into 3 groups, group 1 as negative control group given standard feed, group 2 as positive control group given high-fat diet with a dose of 2.5 ml and group 3 as a treatment group given a high-fat feed with dose of 2.5 ml and extract of bitter melon (<em>Momordica charantia L.)</em> at dose of 37.5 mg / 150 gBB by feeding tube every morning for 28 days. Data were analyzed by T-paired tests.</p><p><strong>Result</strong>: The T-paired test in each group found a significantly different result of p = 0,000.</p><p><strong>Conclusion</strong>: given bitter melon (<em>Momordica charantia L.)</em> extract and high-fat diet can reduce weight in male white rats (<em>Rattus norvegicus</em>).</p><p> </p><p><strong>Keywords</strong>:Bitter molon extract, high fat feed, weight loss.</p><p> </p></div><strong><br clear="all" /></strong>

scholarly journals Effect Administration of Ethanol Extract of Bitter Melon (Momordica Charantia L.) On The Reduction of MDA (Malondialdehyde) Levels Serum in Rats Given a High-Fat Diet

2018 ◽  
Vol 7 (2) ◽  
pp. 196
Author(s):  
Andiani Andiani ◽  
Made Subhawa Harsa
Keyword(s):  
Rattus Norvegicus ◽  
High Fat Diet ◽  
Ethanol Extract ◽  
Momordica Charantia ◽  
The Body ◽  
Control Group ◽  
Bitter Melon ◽  
High Fat ◽  
White Rats ◽  
Ad Libitum

Hyperlipidemia is a disease whichthe effect in increased fat levels in the blood. In hyperlipidemia, the condition of high oxidative stress is obtained due to the oxidation process of LDL (Low Density Lipoprotein) in the body, this condition showed by increased of MDA (Malondialdehyde) levels in serum. Bitter melon (Momordica Charantia L.) contains a lot of active ingredients, one of the ingredients of bitter melon that is thought to have antilipidemic effects is flavonoid compounds. This study was experimental study using a randomized posttest only control group design study with a sample size of 30 male white rats (Rattus norvegicus) wistar strain which was divided into 3 groups, namely K1, K2, and K3. In each group, different treatments are given. Negative control group (K1), given standard feed, water ad libitum; Positive control group (K2), given standard feed, high fat feed with a dose of 2.5 ml water ad libitum; The treatment group (K3) was given a standard diet, high-fat feed with a dose of 2.5 ml water ad libitum and extract of bitter melonat a dose of 250 mg / kg BB by orally every morning for 3 weeks. Data analysis using One Way Anova Test. From the results of the test shows the significance of p-value = 0.000 which is <α (0.05). The results showed that there was an effect of  administration ethanol extract of bitter melon (Momordica charantia L.) to decrease in serum MDA (malondialdehyde) levels in male white wistar rats (Rattus norvegicus) given a high-fat diet.

scholarly journals UJI AKTIVITAS PENURUNAN KADAR ASAM URAT EKSTRAK ETANOL BUAH PARE (Momordica charantia L.) PADA TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI KAFEIN

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 479
Author(s):  
Minar N. S. Gultom ◽  
Edwin De Queljoe ◽  
Elly J. Suoth
Keyword(s):  
Uric Acid ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Momordica Charantia ◽  
Male Rats ◽  
Flavonoid Compound ◽  
Bitter Melon ◽  
White Rats ◽  
Melon Fruit

ABSTRACTBitter melon fruit (Momordica charantia L.) is one of the plants that contains flavonoid compound supposedly as anti-hyperuricemic activity. This study aims to determine the potential activity of reducing uric acid levels from the ethanol extract of bitter melon fruit (Momordica charantia L.) on white wistar male rats (Rattus norvegicus) induced with caffeine. This study is a laboratory experiment using a complete randomized design. A total of 15 rats were divided into 5 groups : negative control (CMC 1%), positive control (Allopurinol), and treatment group of bitter melon fruit (Momordica charantia L.) extract 0,9 mg, 1,8 mg, and 3,6 mg. Hyperuricemic condition was induced by oral administration of  27 mg/200 gr caffeine for 6 days. The uric acid levels was measured before the rats was induced, 6 days after induced and every 3 days of 9 days of treatment. The data obtained were analyzed by ANOVA (Analisis of Variance) and LSD (Least Significant Different). The results show that the dose group of the ethanol extrack of bitter melon fruit had comparable with positive control of Allopurinol in decrease uric acid levels. The conclusion was the ethanol extract of bitter melon fruit (Momordica charantia L.) had anti-hyperuricemic activity in male white rats (Rattus norvegicus) dose 0,9 mg, 1,8 mg and 3,6 mg. Keywords  : Momordica charantia L., antihiperuricemia, Rattus norvegicus, caffeine.ABSTRAK Buah pare (Momordica charantia L.) adalah salah satu tanaman yang mengandung flavonoid yang diduga sebagai senyawa yang mempunyai aktivitas antihiperurisemia. Penelitian ini bertujuan untuk mengetahui potensi aktivitas penurunan kadar asam urat dari ekstrak etanol buah pare pada tikus putih jantan galur wistar (Rattus norvegicus). Jenis penelitian ini ialah eksperimen laboratorium menggunakan Rancangan Acak Lengkap. Sebanyak 15 ekor tikus dibagi dalam 5 kelompok perlakuan : kontrol negatif (CMC 1%), kontrol positif (Allopurinol), dan kelompok dosis uji yaitu pemberian ekstrak etanol buah pare 0,9 mg/200 gr BB; 1,8 mg/200 gr BB; 3,6 mg/200 gr BB. Induksi hiperurisemia dilakukan dengan kafein dosis 27 mg/200 gr selama 6 hari. Pengukuran kadar asam urat dilakukan sebelum induksi, 6 hari setelah diinduksi dan setiap 3 hari selama 9 hari perlakuan. Data yang diperoleh kemudian dianalisis secara statistik menggunakan ANOVA (Analysis of Variance) dan LSD (Least Significant Different). Hasil penelitian menunjukkan bahwa kelompok dosis ekstrak etanol buah pare memiliki aktivitas yang sebanding dengan kontrol positif allopurinol dalam menurunkan kadar asam urat. Kesimpulannya ekstrak etanol buah pare (Momordica charantia L.) memiliki aktivitas antihiperurisemia pada tikus putih jantan (Rattus norvegicus) pada dosis 0,9 mg/200 gr BB; 1,8 mg/200 gr BB; 3,6 mg/200 gr BB. Kata kunci : Momordica charantia L., antihiperurisemia, Rattus norvegicus, kafein.

Antimicrobial activity of Eurycoma longifolia and Momordica charantia

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
R Łos ◽  
K Skalicka-Wozniak ◽  
J Widelski ◽  
A Malm ◽  
K Głowniak
Keyword(s):  
Antimicrobial Activity ◽  
Momordica Charantia ◽  
Eurycoma Longifolia
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