scholarly journals Short-term Momordica charantia L. Fruit Concentrated Infusions Therapy on Alloxan-Induced Rattus norvegicus Kidney Glomerulus Cells Histology

2020 ◽  
Author(s):  
Himmatul Barroroh ◽  
Ayu Dewi ◽  
Hafidatul Hasanah ◽  
Akyunul Akyunul
Keyword(s):  
Rattus Norvegicus ◽  
Momordica Charantia ◽  
Short Term ◽  
Kidney Glomerulus

scholarly journals Evaluation of cytotoxicity and mutagenicity of the benzodiazepine flunitrazepam in vitro and in vivo

2014 ◽  
Vol 50 (2) ◽  
pp. 251-256
Author(s):  
Igor Vivian de Almeida ◽  
Giovana Domingues ◽  
Lilian Capelari Soares ◽  
Elisângela Düsman ◽  
Veronica Elisa Pimenta Vicentini
Keyword(s):  
Rattus Norvegicus ◽  
Bone Marrow Cells ◽  
Micronucleus Test ◽  
Term Treatment ◽  
Genotoxic Effects ◽  
Short Term ◽  
Short Term Treatment ◽  
Chromosomal Aberration Test

Flunitrazepam (FNZ) is a sedative benzodiazepine prescribed for the short-term treatment of insomnia. However, there are concerns regarding possible carcinogenic or genotoxic effects of this medicine. Thus, the aim of this study was to evaluate the cytotoxic, clastogenic and aneugenic effects of FNZ in hepatoma cells from Rattus norvegicus (HTC) in vitro and in bone marrow cells of Wistar rats in vivo. These effects were examined in vitro following treatment with 0.2, 1.0, 5.0 or 10 μg/mL FNZ using a micronucleus test with a cytokinesis block or in vivo using a chromosomal aberration test following treatment with 7, 15 or 30 μg/mL/kg body weight. The results showed that the benzodiazepine concentrations tested were not cytotoxic, aneugenic or clastogenic. However, considering the adverse effects of using this benzodiazepine, more studies are required.

scholarly journals PENGARUH TORSIO TESTIS TERHADAP GANGGUAN PROSES SPERMATOGENESIS PADA TESTIS TIKUS (Rattus norvegicus)

2017 ◽  
Vol 17 (2) ◽  
pp. 84-92
Author(s):  
Nora Adliyanti Basar ◽  
Dahril Dahril ◽  
Tita Menawati ◽  
Fajriah Fajriah
Keyword(s):  
Sertoli Cells ◽  
Rattus Norvegicus ◽  
Testicular Torsion ◽  
Round Spermatid ◽  
Term Effect ◽  
Short Term ◽  
Randomized Design ◽  
Long Term Effect ◽  
Completely Randomized Design

Abstrak. Torsio testis adalah terputarnya atau melilitnya korda spermatika, yang menyebabkan terputusnya aliran darah ke testis dan struktur jaringan di dalam skrotum. Penelitian yang bertujuan untuk mengidentifikasi efek variasi durasi waktu torsio testis terhadap proses spermatogenesis yang meliputi: jumlah sel Sertoli, spermatogonia, spermatosit primer, dan round spermatid testis Rattus norvegicus dilakukan dengan menggunakan rancangan acak lengkap (RAL) dan dibagi dalam 3 kelompok yaitu kontrol (KO) dan perlakuan (P1 dan P2). Kelompok perlakuan diinduksi torsio 360o pada testis kiri selama 4 dan 8 jam. Masing-masing kelompok terdiri dari 5 ekor tikus yang diamati langsung (efek cepat) dan 5 ekor tikus yang diamati setelah 30 hari dilakukan detorsi (efek lambat). Data dianalisa dengan metode ANOVA multifaktorial dan dilanjutkan dengan uji Tuckey HSD. Hasil penelitian menunjukkan bahwa rerata jumlah sel Sertoli pada KO: 142,00 ± 12,36; P1: 48,40 ± 8,00; P2: 35,70 ± 16,28, rerata jumlah spermatogonia KO: 975,30 ± 95,12; P1: 563,70 ± 170,44; P2: 321,10 ± 181,20, rerata jumlah spermatosit primer KO: 1307,50 ± 87,57; P1: 881,50 ± 253,65 ; P2: 505,80 ± 163,69, rerata round spermatid KO: 1237,20 ± 148,75; P1: 766,10 ± 277,68; P2: 473,00 ± 133,25. Berdasarkan hal tersebut, dapat disimpulkan bahwa torsio testis selama 4 dan 8 jam mengakibatkan perubahan jumlah sel Sertoli, spermatogonia, spermatosit primer, dan round spermatid (P0,05). Diharapkan, penanganan segera kasus torsio testis dapat menurunkan angka kerusakan testis.  (JKS 2017; 2: 83-93)Kata kunci : torsio testis, proses spermatogenesis, jumlah sel SertoliAbstract. Testicular torsion is the spermatic cord twisted, which causes the interruption of blood flow to the testicles and structures within the scrotum. The research was subjected to identify the effect of various duration of testicular torsion to spermatogenesis process include on the amount of Sertoli cells, spermatogonia, primary spermatocytes, and spermatids round of Rattus norvegicus testes. This experimental research using completely randomized design (CRD) and contains of 3 groups: control (KO) and experimental groups (P1 and P2). The experimental groups which contains 20 rats underwent 360o unilateral left testicular torsion for 4 hours (P1) and 8 hours (P2). Five rats for each groups were examined after testicular torsion induced (short term effect) besides five other remained were examined after 30 days testicular tortion repaired (long term effect). Data were analyzed using ANOVA multifactorial followed by Tuckey’s HSD test. The result showed significant differences (P0,05). Between KO: 142.00 ± 12.36; P1: 48.40 ± 8.00; and P2: 35.70 ± 16.28 in the amount of Sertoli cells, between KO: 975.30 ± 95, 12: P1: 563.70 ± 170.44; P2: 321.10 ± 181.20 in the amount of spermatogonia, between KO: 1307.50 ± 87.57; P1: 881.50 ± 253.65; P2: 505, 80 ± 163.69 in the amount of primary spermatocytes, between KO: 1237.20 ± 148.75; P1: 766.10 ± 277.68; P2: 473.00 ± 133,25 in the amount of spermatids round. Therefore, testicular torsion for 4 and 8 hours resulted in changes in the amount of Sertoli cells, spermatogonia, primary spermatocytes, and spermatids round (P0,05). Expected, the immediate treatment of cases of testicular torsion may reduce the number of testicular damage. (JKS 2017; 2: 83-93)Keywords: testicular torsion, spermatogenesis process, the amount of Sertoli cell

scholarly journals PENGARUH EKSTRAK DAUN PARE (Momordica charantia) TERHADAP FOLIKEL OVARIUM TIKUS (Rattus norvegicus) DALAM KONDISI HYPERGLYCEMIC

2020 ◽  
Vol 8 (1) ◽  
pp. 61
Author(s):  
Ganeswara Muharam Hazmi Rezady ◽  
Hani Plumeriastuti ◽  
Rimayanti Rimayanti
Keyword(s):  
Treatment Group ◽  
Alternative Treatment ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Momordica Charantia ◽  
Once Daily ◽  
Significant Difference

The purpose of this study was to prove that the provision of pare leaf extract can reduce the process of follicular atrophy in the ovaries caused by hyperglycemic conditions. The total of this study was completed within 20 days, including the induction of Streptozotocin for 5 days, treatment for 14 days, and 1 day for ovarian collection. The K- group as a negative control without being induced by Streptozotocin and received no treatment. The K+ group as a positive control was only induced by Streptozotocin at a dose of 55 mg / Kg BW and D10 without treatment. The T1 group was induced by Streptozotocin at doses of 55 mg / Kg BW and D10 and treated with pare leaves extract (Momordica charantia) at a dose of 1.26 ml / Kg BW. T2 group was induced by Streptozotocin with dose of 55 mg / Kg BW and D10 and was treated with pare leaves extract (Momordica charantia) at a dose of 2.52 ml / Kg BW. Induction of Streptozotocin intraperitoneally and once-daily peroral treatment. The result showed that treatment group could make a significant difference with the positive control. Showing that amount of gap could make Momordica charantia to be alternative treatment for hyperglycemic condition.

scholarly journals Efek Anti Diabetes Buah Pare (Momordica charantia Linn.) Terhadap Kadar Glukosa Darah, Sel Penyusun Pulau Langerhans dan Sel Leydig pada Tikus Putih Hiperglikemia

2017 ◽  
Vol 4 (2) ◽  
pp. 43-50
Author(s):  
I Dewa Putu Anom Adnyana ◽  
Dewa Ketut Meles ◽  
. Wurlina ◽  
Sunarni Zakaria ◽  
Niluh Suwasanti
Keyword(s):  
Rattus Norvegicus ◽  
Momordica Charantia

Penelitian ini bertujuan untuk membuktikan ekstrak buah pare (Momordica charantia Linn.) terhadap kadar glukosa darah, sel penyusun pulau Langerhans dan sel Leydig tikus putih (Rattus norvegicus) hiperglikemia. Tikus putih sebanyak 25 ekor dibagi secara acak menjadi 5 kelompok. Induksi aloksan dengan dosis 150 mg/kgbb secara intraperitoneal untuk menimbulkan kerusakan pankreas dilakukan pada 5 kelompok perlakuan. Tiga kelompok perlakuan diterapi dengan berbagai dosis ekstrak buah pare, (P1) 29 mg/1ml/hari, (P2) 50 mg/1ml/hari, dan (P3) 59 mg/1ml/hari, satu kelompok sebagai kontrol negatif (P0) diberi CMC Na 0,5% 1ml/hari, kontrol positif (K+) diberi Glibenclamide® 0,126 mg/1ml/hari. Ekstrak buah pare diberikan selama 21 hari. Kadar glukosa diperiksa setelah 2 jam, 4 jam, 6 jam dan 8 jam pascapemberian dihari pertama. Kadar glukosa selanjutnya diperiksa pada hari ke 7, 14 dan 21. Semua tikus dieuthanasia setelah 21 hari perlakuan, pankreas dan testis diambil untuk dibuat preparat histopatologi. Hasil menunjukkan bahwa ekstrak buah pare (Momordica charantia Linn.) memiliki efek antidiabetik yang dapat menurunkan kadar gula darah, meningkatkan jumlah sel insula Langerhans dan meningkatkan jumlah sel Leydig pada dosis 50 mg/1ml/hari pada hari ke 21 setelah perlakuan.

scholarly journals Perbandingan kualitas spermatozoa tikus wistar (rattus norvegicus) yang diberi ekstrak buah pare (momordica charantia L) dengan ekstrak biji pepaya (carica papaya L)

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Kyrie E. Wenur ◽  
Lusiana Satiawati ◽  
Lydia Tendean
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Carica Papaya ◽  
Seed Extract ◽  
Momordica Charantia ◽  
Bitter Melon ◽  
Fruit Extract ◽  
Treatment Groups ◽  
Papaya Seeds

Abstract: Male participation in contraception is still lacking. Pare (Momordica charantia L) is known having an inhibitory effect spermatogenesis and seeds of papaya (Carica papaya L) contain the alkaloid compounds that are anti-fertility, both can be used as a male contraceptive. The purpose of this study was to determine sperm quality comparison Wistar rats (Rattus norvegicus) were given a fruit extract of pare (Momordica charantia L) with the seed extract of papaya (Carica papaya L). The research used a completely randomized experimental design. There are nine samples of Wistar rats (Rattus norvegicus) males were divided into three groups and one group consisting three Wistar rats. Over the past 50 days, extracts of pare (Momordica charantia L) is given to the group 1 at 70mg / kg/day, while in the group 2 given seed extract of papaya (Carica papaya L) of 70mg / kg/day and the control group was given no treatment. The results showed significant differences in sperm motility among the treatment groups 1 with extract of bitter melon fruit (Momordica charantia L) and 2 treatment groups with the extract of papaya seeds (Carica papaya L), namely a decrease in sperm motility (p = 0.002). Conclusion, the fruit extract of bitter melon (Momordica charantia L) and seed extract of papaya (Carica papaya L) affects the quality of spermatozoa.Keywords: pare, papaya seeds, quality of spermatozoa. Abstrak: Partisipasi pria dalam menggunakan alat kontrasepsi masih sangat kurang. Pare (Momordica charantia L) diketahui memiliki efek penghambatan spermatogenesis dan biji pepaya (Carica papaya L) mengandung senyawa alkaloid yang bersifat antifertilitas, keduanya dapat digunakan sebagai bahan kontrasepsi pria. Tujuan penelitian ini adalah mengetahui perbandingan kualitas spermatozoa tikus wistar (Rattus norvegicus) yang diberi ekstrak buah pare (Momordica charantia L) dengan ekstrak biji pepaya (Carica papaya L). Penelitian ini menggunakan metode penelitian eksperimental dengan menggunakan rancangan acak lengkap. Sampel yang digunakan adalah sembilan tikus wistar (Rattus norvegicus) jantan yang dibagi menjadi tiga kelompok dan satu kelompok terdiri dari tiga ekor tikus wistar. Selama 50 hari, ekstrak buah pare (Momordica charantia L) diberikan pada kelompok perlakuan 1 sebesar 70mg/kgBB per hari, sedangkan pada kelompok perlakuan 2 diberikan ekstrak biji pepaya (Carica papaya L) sebesar 70mg/kgBB per dan kelompok kontrol tidak diberikan perlakuan. Hasil penelitian menunjukan adanya perbedaan signifikan pada motilitas spermatozoa antara kelompok perlakuan 1 dengan pemberian ekstrak buah pare (Momordica charantia L) dan kelompok perlakuan 2 dengan pemberian ekstrak biji pepaya (Carica papaya L) yaitu penurunan motilitas spermatozoa ( p = 0,002).Kesimpulan, ekstrak buah pare (Momordica charantia L) dan ekstrak biji pepaya (Carica papaya L) berpengaruh terhadap kualitas spermatozoa. Kata kunci: buah pare, biji pepaya, kualitas spermatozoa

scholarly journals Pemberian Ekstrak Buah Pare dan Diet Tinggi Lemak pada Penurunan Berat Badan Tikus Putih Jantan

2019 ◽  
Vol 17 (1) ◽  
pp. 60
Author(s):  
I MADE SUBHAWA HARSA
Keyword(s):  
Rattus Norvegicus ◽  
High Fat Diet ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Momordica Charantia ◽  
Control Group ◽  
Bitter Melon ◽  
High Fat ◽  
White Rats

<div class="Section1"><p align="center"><strong>Abstra</strong><strong>ct</strong></p><p><strong>Background</strong> : Obesity is an energy balance disorder, ketogenic diet can reduce body weight. Bitter melon (<em>Momordica charantia L.)</em> is a type of vegetable material that is often used as an antioxidant, hypocholesterolemia and hypotriglyceridemia drug. <em>Cucurbitasin</em> is a substance produced by bitter melon (<em>Momordica charantia L.)</em> with a bitter taste which causes in decreasing appetite, resulting in a decrease in triglycerides. This is caused by the bioactivity of bitter melon (<em>Momordica charantia L.)</em> which can increase lipid oxidation, which causes the accumulation of decreased fat tissue which can reduce weight.</p><p><strong>Methods</strong>: This research using pre and post test randomized controlled group design with sample size of 30 male white rats (<em>Rattus norvegicus</em>) wistar lines divided into 3 groups, group 1 as negative control group given standard feed, group 2 as positive control group given high-fat diet with a dose of 2.5 ml and group 3 as a treatment group given a high-fat feed with dose of 2.5 ml and extract of bitter melon (<em>Momordica charantia L.)</em> at dose of 37.5 mg / 150 gBB by feeding tube every morning for 28 days. Data were analyzed by T-paired tests.</p><p><strong>Result</strong>: The T-paired test in each group found a significantly different result of p = 0,000.</p><p><strong>Conclusion</strong>: given bitter melon (<em>Momordica charantia L.)</em> extract and high-fat diet can reduce weight in male white rats (<em>Rattus norvegicus</em>).</p><p> </p><p><strong>Keywords</strong>:Bitter molon extract, high fat feed, weight loss.</p><p> </p></div><strong><br clear="all" /></strong>

Blood sampling from the cranial vena cava in the Norway rat (Rattus norvegicus)

2005 ◽  
Vol 39 (2) ◽  
pp. 236-239 ◽  
Author(s):  
Vladimír Jekl ◽  
Karel Hauptman ◽  
Edita Jeklová ◽  
Zdeněk Knotek
Keyword(s):  
Injection Site ◽  
Rattus Norvegicus ◽  
Jugular Vein ◽  
Vena Cava ◽  
The Body ◽  
Blood Sampling ◽  
Short Term ◽  
Norway Rat ◽  
Limit Stress ◽  
The Given

This paper describes blood sampling from the cranial vena cava (CVC) in the Norway rat. In order to limit stress, the blood sampling should be done under short-term inhalation anaesthesia, for example, an oxygen/isoflurane mixture. The injection site is just cranial to the first rib, 0.3–0.8 cm lateral to the manubrium when the animal is in dorsal recumbency. The needle, attached to a syringe, is inserted at 30° in the direction of the opposite femoral head. After penetration of the skin, negative pressure is developed in the syringe and the insertion of the needle is continued for another 0.2–1 cm in the given direction until blood begins to flow. The amount of blood sampled ranges from 0.8 to 2.5 mL depending on the body weight of the patient. A trial on 50 rats aged 5–24 months included 25 rats sampled once, eight rats sampled twice with an interval of seven days, 11 rats sampled twice with an interval of three weeks and four rats sampled four times with intervals of four weeks – a total of 87 blood samplings. The serious complications quoted in association with blood sampling from the CVC in other experimental animals (vascular lacerations, heart puncture, serious haemorrhage, tracheal and throat trauma) were not observed in our study. There were only four blood samplings (4.5%) with mild haemorrhage from the injection site, due to erroneous sampling from the jugular vein.

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