scholarly journals Protocorm Like Bodies (PLB) Anggrek Hasil Silangan Phalaenopsis gigantea × Phalaenopsis violacea pada Kombinasi Media dan ZPT

2015 ◽  
Vol 5 (2) ◽  
pp. 118
Author(s):  
Sandra Arifin Aziz ◽  
Dewi Sukma ◽  
, Nazi

<p>ABSTRACT</p><p>The study  was  aimed to  determine  combination media and plant growt regulator to support growth  of  F1  of  PLB  orchid  from  Phalaenopsis gigantea x Phalaenopsis  violacea  hibridisation,. This research consist of two experiment. The first  experiment use  randomize  complete  block  design with two factor i.e media and plant growth regulator (PGR). The experiment used Knudson C 80% and  NPK  (18:18:18+EDTA)  media, while  the plant  growth  regulator used without  PGR,  coconut water, thidiazuron (TDZ)  +  benziladenin (BA). The second experiment  was  subculture in different media,  Knudson  C  80% without  PGR  to  Knudson  C  80%  without  PGR  and  NPK, NPK (20:20:20)+vitamin+myoinositol; NPK (18-18-18+EDTA)  without PGR subculture to  NPK (18-18-18+EDTA)  without  PGR  and  NPK (20:20:20)+vitamin+myoinositol;  NPK  (18:18:18+EDTA) +TDZ 0.5 ppm+BA 0.5 ppm subculture to NPK (18:18:18+EDTA) +TDZ 0.5 ppm+BA 0.5 ppm andNPK  (20:20:20)+  vitamin+myoinositol;  KC  80%+coconut  water  100 ml, KC  80%+TDZ  0.5 ppm+BA  0.5  ppm  and  NPK  (18:18:18+EDTA) +coconut water  100  ml  subculture  to  NPK (20:20:20)+  vitamin+myoinositol.  The result showed that the best combination of media and plant growth  regulator to proliferation was NPK (18:18:18+EDTA) without PGr media, and then subculture in the same media i.e NPK (20:20:20) +vitamin+ myoinositol.</p><p>Key words: Knudson C 80%, Protocorm Like Bodies (PLB), orchid, thidiazuron,benziladenin</p><p> </p><p>ABSTRAK</p><p>Penelitian ini bertujuan untuk mempelajari kombinasi media tanam dan  zat pengatur tumbuh(ZPT)  yang sesuai untuk mendukung pertumbuhan PLB anggrek hasil silangan antara  Phalaenopsisgigantea  ×  Phalaenopsis  violacea. Penelitian  terdiri  atas  2  percobaan. Percobaan 1  menggunakan rancangan acak  lengkap  faktorial  dengan  2  faktor,  yaitu  media  tanam  dan  ZPT. Media  yang digunakan adalah media Knudson C (KC) 80% dan media NPK (18:18:18+EDTA), sedangkan ZPT yang dicobakan adalah tanpa penambahan ZPT, air kelapa, serta  thidiazuron (TDZ)  +benziladenin (BA).  Percobaan  2 adalah  subkultur  pada  media  yang  berbeda  menggunakan  rancangan acak lengkap  yaitu,  KC  80%  tanpa  ZPT  disubkultur  ke  KC  80%  tanpa ZPT  dan  NPK (20:20:20)+vitamin+  myoinositol; NPK (18-18-18+EDTA) tanpa ZPT disubkultur ke NPK  (18-18-18+EDTA) tanpa ZPT dan NPK (20:20:20)+vitamin+myoinosi tol; NPK (18:18:18+EDTA) +TDZ 0.5 ppm+BA 0.5 ppm disubkultur ke NPK (18:18:18+EDTA) +TDZ 0.5 ppm+BA 0.5 ppm dan NPK (20:20:20)+vitamin +myoinositol; KC 80%+air kelapa 100 ml, KC 80%+TDZ 0.5 ppm+BA 0.5 ppm dan  NPK  (18:18:18+EDTA)  +air  kelapa 100 ml  disubkultur  ke  NPK  (20:20:20)+vitamin+myoinositol.  Hasil  percobaan menunjukkan  bahwa  perlakuan  media  dan  ZPT  terbaik  untuk proliferasi adalah  media  NPK  (18:18:18+EDTA)  tanpa  penambahan  ZPT,  kemudian disubkultur pada media yang sama, atau media NPK (20:20:20) +vitamin+ myoinositol.</p><p>Kata kunci: Knudson C 80%, Protocorm Like Bodies (PLB), anggrek, thidiazuron, benziladenin</p>

2017 ◽  
Vol 8 (1) ◽  
pp. 46
Author(s):  
Isna Tustiyani

Kopi merupakan salah satu komoditas perkebunan unggulan nasional. Pembibitan kopi umumnya melalui biji, padahal kopi dapat dibudidayakan melalui cara vegetative yaitu stek asal diberi perlakuan at pengatur tumbuh. Tujuan penelitian  ini adalah untuk  mempelajari pengaruh pemberian berbagai zat pengatur tumbuh alami pada stek kopi. Percobaan dilaksanakan di Kebun Percobaan  Fakultas Pertanian, Universitas Garut pada Oktober – November 2016 menggunakan Rancangan Acak Kelompok (RAK) dengan perlakuan tanpa pemberian ZPT, growtone, ekstrak bawang merah, dan air kelapa. Hasil percobaan dapat disimpulkan bahwa pemberian ekstrak bawang merah dapat menumbuhkan sebesar 50% dan penggunaan growtone sebesar 55% stek kopi hidup.KATA KUNCI: auksin, air kelapa, ekstrak bawang merah,  stek kopi  THE EFFECT OF VARIOUS NATURAL PLANT GROWTH REGULATOR ON GROWTH CUTTINGS OF COFFEEABSTRACTThe Coffee is one of national excellent commodities. The Nurseries of coffee generally through the beans, but it can be grown through vegetative through by cuttings treated with growth regulators. The purpose of this research was to study the effect of various naturally plant growth regulators in coffee cuttings. The experiment was conducted at the Experimental Farm, Faculty of Agriculture, University of Garut in October-November, 2016 using Randomized Complete Block Design with treatment  without giving plant growth regulator, growtone, onion extract, and coconut water. The result showed  that the extract of onion can grow at 50% and the growtone treatments  can grow 55% of the coffee cuttings. 


2019 ◽  
Vol 19 (2) ◽  
pp. 277
Author(s):  
Eltis Panca Ningsih ◽  
Imas Rohmawati

Abstrak : Tanaman miana saat ini dimanfaatkan sebagai tanaman hias. Perbanyakan Tanaman miana dapat dilakukan dengan cara generatif maupun vegetatif. Stek pucuk merupakan cara perbanyakan vegetatif tanaman miana yang relatif mudah dilakukan. Pembibitan dengan cara ini merupakan salah satu cara cepat dalam memenuhi kebutuhan bahan tanaman skala besar. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian Zat Pengatur Tumbuh terhadap stek pucuk tanaman miana (Coleus atropurpureus (L.) Benth. Penelitian dilaksanakan di di laboratorium agroekologi, Fakultas Pertanian, Universitas Sultan Ageng Tirtyasa. Penelitian akan dilaksanakan pada bulan pada bulan Maret sampai Mei 2017. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) yang yang terdiri dari empat perlakuan dan diulang tiga kali. Perlakuan yang diuji dalam percobaan ini adalah: kontrol (Tanpa ZPT), IAA 750 ppm, Growtone 500 ppm, dan air kelapa 10%. Hasil penelitian menunjukkan bahwa pemberian zat pengatur tumbuh berpengaruh terhadap jumlah tunas dan jumlah akar. Jumlah tunas terbanyak terdapat pada perlakuan Growtone sebesar 7.50 tunas. Jumlah akar terbanyak terdapat pada perlakuan IAA sebesar 91.00 buah. Rekomendasi budidaya tanaman miana dengan cara stek dapat menggunakan IAA 750 ppm maupun Growtone 500 ppm.Kata Kunci : miana, stek, zat pengatur tumbuhAbstract : Miana plant is currently used as an ornamental plant. The multiplication of miana crops can be done in a generative and vegetative way. Cuttings is a vegetative way of reproduction of miana plant which is relatively easy to do. Breeding in this way is one of the quick ways in meeting the needs of large-scale plant materials. This research aims to determine the effect of giving Plant Growth Regulator to the shoots cuttings of Miana (Coleus atropurpureus (L.) Benth). Research conducted in the Agroecological laboratory, Faculty of Agriculture, University of Sultan Ageng Tirtyasa. The research will be held in the month of March to May 2017. The study conducted by randomized block design (RBD) which consists of four treatments and is repeated three times. The treatment tested in this experiment was: control (without plant growth regulator), IAA 750 ppm, Growtone 500 ppm, and 10% coconut water. The results showed that the treatment of plant growth regulator affected in the number of shoots and the number of roots. The most number of shoots is in the Growtone treatment of 7.50 shoots. The highest number of roots is in the IAA treatment of 91.00 pieces. Recommendations for cultivation of miana crops with cuttings can use IAA 750 ppm maupun Growtone 500 ppm.Keyword : Miana, cuttings, growing regulatory substances 


1984 ◽  
Vol 64 (2) ◽  
pp. 225-228 ◽  
Author(s):  
G. CACCO ◽  
G. DELL’AGNOLA

Auxin and cytokinin bioassays were performed to test the biological activity of soluble humic complexes (SHC). "Pea split stem curvature" (auxin test) and "cucumber" tests (cytokinin test) did not show any quantifiable biological activity of SHC. On the contrary, "cress test" and "senescence test" offered good evidence of a hormonal-like activity 100 times lower than that of IAA (10 mg IAA∙g−1 SHC) and 10 times lower than that of N6 BA (100 mg N6 BA∙g−1 SHC). At a high concentration of SHC, toxic effects were evident, indicating the presence of inhibitory substances which counteracted the hormone-like activity of humic complexes. Key words: Plant growth regulators, soluble humic complexes


2021 ◽  
Vol 1908 (1) ◽  
pp. 012006
Author(s):  
Elik Murni Ningtias Ningsih ◽  
Sudiyono ◽  
Frida Dwi Anggraeni

2016 ◽  
Vol 3 (2) ◽  
pp. 90-98
Author(s):  
Pienyani Rosawanti

This research aims to study the growth response of plant leaf cuttings mother in-law�s tongue (Sansevieria parva) origin of cuttings and various plant growth regulator treatment. Research was using complete randomized block design (RAKL) with 6 replications. The first factor was the origin of cuttings, i.e. the top/tip leaves, the middle leave, and the bottom/leaves base. The second factor was the various plant growth regulator (PGR) treatment i.e. onion extracts, water coconut and Rootone-F. Parameters observed and measured is the experiment root length, number of roots, root wet weight and root dry weight. The results showed that plant growth regulator treatment significant effect on root length, number of roots, root wet weight, and root dry weight. The treatment combination of origin of cuttings and synthetic plant growth regulator by cutting material from the middle to give the best results on the number of root and root wet weight.


2021 ◽  
Vol 30 (2) ◽  
pp. 125
Author(s):  
Zulfa Rahmadita Nur Azizah ◽  
Etik Wukir Tini ◽  
Joko Maryanto

<p>Perbanyakan tanaman secara vegetatif menggunakan teknik sambung pucuk dapat digunakan sebagai alternatif untuk menghasilkan benih yang bermutu. Namun, masalah yang sering terjadi pada sambung pucuk adalah kegagalan sambung. Penelitian ini bertujuan untuk mendapatkan jenis zat pengatur tumbuh, jumlah daun entres, dan kombinasi perlakuan yang memberikan hasil terbaik terhadap keberhasilan sambung pucuk durian. Penelitian dilaksanakan pada bulan November 2018 sampai Januari 2019 di Desa Alasmalang, Kemranjen, Banyumas. Percobaan yang dilakukan merupakan percobaan pot dengan rancangan faktorial. Perlakuan pada penelitian ini adalah kombinasi antara zat pengatur tumbuh (kontrol, ekstrak tauge, air kelapa, IBA, dan BAP) dan jumlah daun entres (2, 4, dan 6 helai). Rancangan penelitian yang digunakan yaitu Rancangan Acak Kelompok Lengkap dengan 15 perlakuan dan diulang tiga kali. Hasil penelitian menunjukkan perlakuan ZPT IBA dan BAP memberikan pengaruh nyata terhadap jumlah tunas sambung pucuk tanaman durian, yaitu sebesar 2,711 dan 2,822 dan perlakuan jumlah daun entres dua helai memberikan pengaruh yang nyata terhadap waktu pecah tunas, jumlah tunas, dan pertambahan jumlah daun sambung pucuk tanaman durian, yaitu sebesar 2,3 helai.</p><p><strong>Keywords</strong></p><p>Sambung pucuk durian; Daun entres; Zat pengatur tumbuh</p><p><strong>Abstract</strong></p><p>Vegetative propagation of plants using shoot grafting can be used as an alternative to produce quality seeds. The problem that often occours in top grafting is the failure to graft. This experiment aims to obtain the type of plant growth regulator, the number of scion’s leaves, and the combination that give the best results for growth of top grafting in durian. The research was conducted in November 2018 to January 2019 in Alasmalang Village, Kemranjen, Banyumas. The experiment was a pot experiment with a factorial design. The treatment was a combination of growth regulator (control, bean extract, coconut water, IBA, and BAP) and number of scion leaves (2, 4, and 6 strands). The research design used was a RCBD with 15 treatments and three replications. The results showed that the treatment of PGR IBA and BAP had a significant effect on the number of grafted shoots of durian plants, namely 2.711 and 2.822 and the treatment of the number of leaves of two leaves had a significant effect on shoot break time, the number of shoots and the increase in the number of grafted leaves of durian plants, namely amounting to 2.3 strands.</p>


Author(s):  
Victoria Otie ◽  
AN Ping ◽  
Ali Ibrahim ◽  
Egrinya Eneji

Aim: To assess the pleiotropic role of a plant growth regulator, commercially identified as brassinolide (BR) in mitigating waterlogging stress imposed on maize. Study Design: A factorial combination of two maize varieties [Ikom White (IKW) and Oba-98], two BR levels (0 and 250 ml) and two waterlogging stages of maize growth [control (WL0) and seedling stage (WL1)], arranged as a split-split plot in a randomized complete block design with three replications was used. Place and Duration of Study: Akpabuyo Local Government Area, Cross River State-Nigeria. A two-year field experiment was conducted during the dry seasons of December 2016 and December 2017. Methodology: Waterlogging test was conducted on plots by demarcating them with 3.6 by 1.7 m metal sheets buried to a depth of 60 cm to prevent lateral soil-water movement. Two maize seeds were sown at 25 cm within and 75 cm between rows. The BR (250 ml) was sprayed foliar at 21 DAS. The non-waterlogging plots served as control. Observations were made on growth and yield variables as well as the plant's physiological traits. Results: Waterlogging significantly reduced the growth attributes of maize and increased (p≤0.05) the leaf moisture content. The photosynthetically active radiation on maize plants was substantially reduced (p≤0.05) by the waterlogging stress. Dry matter yield (DMY) and nutrient uptake in the leaves, stems and grains were reduced (p≤0.05) at both silking and at harvest. The effect of the BR was greater in Oba-98 with higher nutrient contents, radiation absorption, dry matter and grain yields than IKW. Conclusion: Treatment of maize plants with BR could induce some tolerance of field waterlogging. Thus, for optimum efficiency in maize production under stressed soil condition of waterlogging, it is recommended that the foliar spray of BR at the 250 ml per plant rate be considered.


2021 ◽  
Vol 4 (2) ◽  
pp. 130-138
Author(s):  
Silvana Prameswari ◽  
Bayu Pratomo

Introduction: This study aims to obtain concentration of shallot extract (Allium cepa ) and growth regulators to increase growth of Mucuna bracteata. Mucuna is a land cover plant that must be planted in oil palm plantation areas. It is very important to be able to cover the planting area. Materials and Methods: This study used two factorial in randomized block design. The first was concentration of shallot extract per 100 ml distilled water consists of 4 levels, such as control (B0), 10 cc (B1), 20 cc (B2), 30 cc (B3) and the second was the auxins-plant growth regulator consists of 4 levels: Control (G0), 100 ppm (G1), 200 ppm (G2), 300 ppm (G3). Data analyzed with ANOVA with a significant 5% and continued with the DMRT using SAS version 9.1.3. Results: It was found that effect of concentration of shallot extract had a significant effect on survival percentage of Mucuna bracteata at a concentration of 10 cc (B2) after opening the lid at four weeks after planting (WAP) and did not change until 8 WAP. Likewise, the number of leaves at the control concentration (B0) at the age of 6 WAP, and a concentration of 10 cc (B1) at the age of 7-8 WAP. The concentration of growth regulators and their interactions did not significantly affect the survive percentage and number of leaves in Mucuna bracteata.


2019 ◽  
Vol 2 (1) ◽  
pp. 14-19
Author(s):  
K. Kasriana ◽  
Marlina Mustafa ◽  
Yolanda Fitria Syahri

Pepper is an important crop in Indonesia because it is one of the country's foreign exchange sources because it is one of the export commodities. The availability of good plant materials will support increased production. Provision of growth regulators in vegetative propagation is very influential on the propagation of pepper plants. The purpose of this study was to determine the most effective combination of growth regulators for pepper cutting. This study was conducted using a randomized block design consisting of eight treatments namely Control (P1), metallic 2 mL L-1 water (P2), mastafol 2 mL (p3), Atonik 2 mL (P4), metallic 1 mL + Mastafol 1 mL (P5), metallic 1 mL + atonic 1 mL (P6), mastafol 1 mL + atonic 1 mL (P7), metallic 0.66 mL + Mastafol 0.66 mL + Atonic 0.66 mL (P8). Setek is grown on soil media: fuel husk: manure (2: 1: 1). The results showed that the best  Plant growth regulator  combination treatment for the growth of pepper cuttings was P7 (mastafol 1 mL L-1 water + atonic 1 mL L-1 water), which can be seen in the parameters of the number of roots, root length, root volume and number of shoots. The treatment of  Plant growth regulator  given did not significantly affect the character of leaf growth


DYNA ◽  
2021 ◽  
Vol 88 (217) ◽  
pp. 75-83
Author(s):  
Leonardo Vieira de Sousa ◽  
Toshik Iarley da Silva ◽  
Maria de Fátima de Queiroz Lopes ◽  
Márcia Paloma da Silva Leal ◽  
Ana Gabriela Sousa Basilio ◽  
...  

The water in semiarid regions contains salts in excess. When good quality water is not available it is necessary to use strategies that can make feasible the use of saline water. The aim of this study was to evaluate the effects of the application of the plant growth regulator on the culture of basil stressed with salt. The experiment was carried out in a randomized block design by the matrix “Central Box Compound”. Shoot height, stem diameter, leaf area, dry masses and soil chemical characteristics were evaluated. The salinity of the irrigation water causes reduction of the basil growth parameters, with the exception of the inflorescence dry mass. Plant growth regulator applications have effect on basil growth when plants are irrigated with saline water.


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