scholarly journals Effects of Different Levels of Pistachio by-product on In vitro Ruminal Fermentation and Performance of Rayini Goats

2020 ◽  
Vol 11 (27) ◽  
pp. 18-26
Author(s):  
Aazam Mirheidari ◽  
Yousef Rouzbahan ◽  
Hassan Fazael ◽  
◽  
◽  
...  
Keyword(s):  
Ruminal Fermentation ◽  
And Performance ◽  
Different Levels

Can a blend of integrated feed additives modulate ruminal fermentation patterns and performance of growing lambs? In vitro and in vivo assessments

2021 ◽  
pp. 1-12
Author(s):  
Amr E. El-Nile ◽  
Amr S. Morsy ◽  
Hani M. El-Zaiat ◽  
Wael G. Fahmy ◽  
Alaa E. El-Komy ◽  
...  
Keyword(s):  
Feed Additives ◽  
Ruminal Fermentation ◽  
And Performance

In vitro degradability of different fractions of Solanum lycocarpum St Hil

2009 ◽  
Vol 2009 ◽  
pp. 90-90
Author(s):  
H R Lima Neto ◽  
A S Chaudhry ◽  
M M H Khan ◽  
M R Virk
Keyword(s):  
Dry Matter ◽  
Production Systems ◽  
Scientific Investigation ◽  
Feed Additive ◽  
Ruminal Fermentation ◽  
Alternative Feed ◽  
Specific Species ◽  
Chrysocyon Brachyurus ◽  
Different Levels

Naturally grown in the vast majority of Brazilian territory, Solanum lycocarpum (SL, popularly known as wolf’s apple) can be used as alternative feed additive for ruminants. Despite its significant amount of secondary metabolites, it is known to be eaten by cattle and a specific species of wild wolf (Chrysocyon brachyurus). However the effects of these organic compounds on ruminal fermentation are still unclear and a dose-response is expected to turn a potential feedstock into an undesirable baneful aliment (Goel et al., 2008). As a result scientific investigation regarding this highly promising plant is needed to test its potential as a supplement to support sustainable livestock production systems. Thus this study compared the effects of different dried meal-like fractions (fruit, leaf, flower, root, stem) of this plant at two different levels (0.2 and 0.4 g) on the in vitro dry matter (DM) degradability (IVD) at three different times of incubation.

The effects of three total mixed rations with different concentrate to maize silage ratios and different levels of microalgae Chlorella vulgaris on in vitro total gas, methane and carbon dioxide production

2016 ◽  
Vol 155 (3) ◽  
pp. 494-507 ◽  
Author(s):  
A. E. KHOLIF ◽  
M. M. Y. ELGHANDOUR ◽  
A. Z. M. SALEM ◽  
A. BARBABOSA ◽  
O. MÁRQUEZ ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Chlorella Vulgaris ◽  
Dry Matter ◽  
Carbon Dioxide Production ◽  
Optimal Level ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Maize Silage ◽  
Different Levels

SUMMARYThe aim of the current study was to assess the effects of adding Chlorella vulgaris algae at different levels on in vitro gas production (GP) of three total mixed rations (TMR) with different concentrate (C): maize silage (S) ratios (25C : 75S, 50C : 50S, 75C : 25S). Chlorella vulgaris was added at 0, 20, 40 and 80 mg/g dry matter (DM) of the TMR and total gas, methane (CH4) and carbon dioxide (CO2) production were recorded after 2, 4, 6, 8, 10, 12, 24 and 48 h of incubation in three runs. Increasing concentrate portion in the TMR linearly increased the asymptotic GP and decreased the rate of GP without affecting the lag time. Addition of C. vulgaris at 20 mg/g DM to the 25C : 75S TMR increased the asymptotic GP, CH4, CO2 and GP at 48 h. Addition of C. vulgaris to the 50C : 50S TMR decreased the asymptotic GP and GP at 48 h. Higher CH4 production was observed at 48 h of incubation when C. vulgaris was included at (per g DM): 20 mg for the 25C : 75S ration, 40 mg for the 50C : 50S ration and 80 mg for the 75C : 25S ration. Inclusion of C. vulgaris linearly increased CH4 production for the 50C : 50S ration and increased CO2 production at 10 and 12 h of incubation for the 50C : 50S ration, whereas 20 and 40 mg C. vulgaris/g DM of the 75C : 25S TMR decreased CO2 production. The 25C : 75S TMR had the highest in vitro DM disappearance with C. vulgaris addition. Chlorella vulgaris addition was more effective with rations high in fibre content than those high in concentrates. It can be concluded that the optimal level of C. vulgaris addition was 20 mg/g DM for improved ruminal fermentation of the 25C : 75S TMR.

scholarly journals Influence of different levels of dried citrus pulp on in vitro ruminal fermentation kinetics of total mixed ration in goat rumen inocula

2012 ◽  
Vol 21 (3) ◽  
pp. 458-467 ◽  
Author(s):  
J. Hernández ◽  
R. Rojo ◽  
A. Salem ◽  
F. Mirzaei ◽  
A. Gonzalez ◽  
...  
Keyword(s):  
Ruminal Fermentation ◽  
Total Mixed Ration ◽  
Fermentation Kinetics ◽  
Citrus Pulp ◽  
Kinetics Of ◽  
Different Levels ◽  
Goat Rumen

In vitro ruminal digestion of anthocyanidin-containing alfalfa transformed with the maize Lc regulatory gene

2006 ◽  
Vol 86 (4) ◽  
pp. 1119-1130 ◽  
Author(s):  
Y. Wang ◽  
P. Frutos ◽  
M. Y. Gruber ◽  
H. Ray ◽  
T. A. McAllister
Keyword(s):  
Dry Matter ◽  
Initial Rate ◽  
Regulatory Gene ◽  
Light Conditions ◽  
Ruminal Fermentation ◽  
Utilization Of Protein ◽  
Similar Content ◽  
Ruminal Digestion ◽  
Different Levels

In vitro ruminal digestion was conducted on novel “purple-green” alfalfa forage that accumulated anthocyanidin and proanthocyanidins through the expression of a maize bHLH anthocyanidin regulatory gene (Lc). The Lc-transgenic genotypes were compared with parental (non-transformed, NT) alfalfa in their in vitro ruminal fermentation, dry matter (DM) and N disappearances, and DM degradability. The transgenic genotypes expressed the Lc gene at different levels when grown under high light conditions, resulting in anthocyanidin contents as high as 136 g g-1 DM. Lc genotypes had lower true DM disappearance than NT alfalfa at 0, 4 and 12 h of incubation, but not at 24 or 48 h. Compared with NT, Lc-transgenic genotypes had a lower content of rapidly soluble DM, but a similar content and rate of degradation of the slowly degradable DM fraction, and a similar lag time for digestion. True disappearance of N was lower for Lc- transgenic than NT alfalfa at the initiation of the incubation. The solubility of both DM and N were negatively correlated with the concentration of anthocyanidins measured in the forage. The results indicate that Lc-transformation reduced the initial rate, but not the extent of DM and N digestion of alfalfa in the rumen. These properties could improve the utilization of protein and possibly reduce the risk of bloat in ruminants consuming fresh alfalfa. However, further increasing the amount of anthocyanidins (or proanthocyanidins) produced in the forage may be required to make this a viable strategy for improved protein utilization and bloat prevention. Key words: Alfalfa, anthocyanidins, Lc-transformation, nitrogen, ruminal digestion

Immunoenzymatic demonstration of the simultaneous presence of transferrin, hemopexin and albumin in a same hepatocyte and their ultrastructural localization

1978 ◽  
Vol 36 (2) ◽  
pp. 88-89
Author(s):  
M. Kraemer ◽  
J. Foucrier ◽  
J. Vassy ◽  
M.T. Chalumeau
Keyword(s):  
Plasma Proteins ◽  
Rat Hepatocytes ◽  
Ultrastructural Localization ◽  
Carrier Proteins ◽  
Normal Cells ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Monospecific Antisera ◽  
Different Levels

Some authors using immunofluorescent techniques had already suggested that some hepatocytes are able to synthetize several plasma proteins. In vitro studies on normal cells or on cells issued of murine hepatomas raise the same conclusion. These works could be indications of an hepatocyte functionnal non-specialization, meanwhile the authors never give direct topographic proofs suitable with this hypothesis.The use of immunoenzymatic techniques after obtention of monospecific antisera had seemed to us useful to bring forward a better knowledge of this problem. We have studied three carrier proteins (transferrin = Tf, hemopexin = Hx, albumin = Alb) operating at different levels in iron metabolism by demonstrating and localizing the adult rat hepatocytes involved in their synthesis.Immunological, histological and ultrastructural methods have been described in a previous work.

The Safety and Antimicrobial Properties of Stabilized Hypochlorous Acid in Acetic Acid Buffer for the Treatment of Acute Wounds—a Human Pilot Study and In Vitro Data

2021 ◽  
pp. 153473462110156
Author(s):  
Ewa A. Burian ◽  
Lubna Sabah ◽  
Klaus Kirketerp-Møller ◽  
Elin Ibstedt ◽  
Magnus M. Fazli ◽  
...  
Keyword(s):  
Pilot Study ◽  
Hypochlorous Acid ◽  
Treatment Time ◽  
Donor Site ◽  
Antimicrobial Properties ◽  
Prolonged Treatment ◽  
Wound Irrigation ◽  
The Mean ◽  
And Performance

Acute wounds may require cleansing to reduce the risk of infection. Stabilized hypochlorous acid in acetic buffer (HOCl + buffer) is a novel wound irrigation solution with antimicrobial properties. We performed a first-in-man, prospective, open-label pilot study to document preliminary safety and performance in the treatment of acute wounds. The study enrolled 12 subjects scheduled for a split-skin graft transplantation, where the donor site was used as a model of an acute wound. The treatment time was 75 s, given on 6 occasions. A total of 7 adverse events were regarded as related to the treatment; all registered as pain during the procedure for 2 subjects. One subject had a wound infection at the donor site. The mean colony-forming unit (CFU) decreased by 41% after the treatment, and the mean epithelialization was 96% on both days 14 (standard deviation [SD] 8%) and 21 (SD 10%). The study provides preliminary support for the safety, well-tolerance, and efficacy of HOCl + buffer for acute wounds. The pain was frequent although resolved quickly. Excellent wound healing and satisfying antimicrobial properties were observed. A subsequent in vitro biofilm study also indicated good antimicrobial activity against Pseudomonas aeruginosa with a 96% mean reduction of CFU, when used for a treatment duration of 15 min ( P < .0001), and a 50% decrease for Staphylococcus aureus ( P = .1010). Future larger studies are needed to evaluate the safety and performance of HOCl + buffer in acute wounds, including the promising antimicrobial effect by prolonged treatment on bacterial biofilms.

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