scholarly journals In Vivo Biodistribution, PET Imaging, and Tumor Accumulation of 86Y- and 111In-Antimindin/RG-1, Engineered Antibody Fragments in LNCaP Tumor-Bearing Nude Mice

2009 ◽  
Vol 50 (3) ◽  
pp. 435-443 ◽  
Author(s):  
D. W. Schneider ◽  
T. Heitner ◽  
B. Alicke ◽  
D. R. Light ◽  
K. McLean ◽  
...  
Keyword(s):  
Nude Mice ◽  
Pet Imaging ◽  
Antibody Fragments ◽  
Tumor Bearing ◽  
In Vivo Biodistribution ◽  
Tumor Accumulation

scholarly journals Biodistribution Study of Niosomes in Tumor-Implanted BALB/C Mice Using Scintigraphic Imaging

2022 ◽  
Vol 12 ◽  
Author(s):  
Leanne De Silva ◽  
Ju-Yen Fu ◽  
Thet Thet Htar ◽  
Wan Hamirul Bahrin Wan Kamal ◽  
Azahari Kasbollah ◽  
...  
Keyword(s):  
Intravenous Injection ◽  
Gamma Scintigraphy ◽  
Biodistribution Study ◽  
Scintigraphic Imaging ◽  
Tumor Bearing ◽  
Tumor Accumulation ◽  
Tumor Area

The purpose of this work was to study the biodistribution of niosomes in tumor-implanted BALB/c mice using gamma scintigraphy. Niosomes were first formulated and characterized, then radiolabeled with Technetium-99 m (99mTc). The biodistribution of 99mTc-labeled niosomes was evaluated in tumor-bearing mice through intravenous injection and imaged with gamma scintigraphy. The labeled complexes possessed high radiolabeling efficiency (98.08%) and were stable in vitro (>80% after 8 h). Scintigraphic imaging showed negligible accumulation in the stomach and thyroid, indicating minimal leaching of the radiolabel in vivo. Radioactivity was found mainly in the liver, spleen and kidneys. Tumor-to-muscle ratio indicated a higher specificity of the formulation for the tumor area. Overall, the formulated niosomes are stable both in vitro and in vivo, and show preferential tumor accumulation.

scholarly journals Synthesis and evaluation of novel F-18-labeled pyrimidine derivatives: potential FAK inhibitors and PET imaging agents for cancer detection

RSC Advances ◽  
2017 ◽  
Vol 7 (36) ◽  
pp. 22388-22399 ◽  
Author(s):  
Dawei Wang ◽  
Yu Fang ◽  
Hang Wang ◽  
Xingyu Xu ◽  
Jianping Liu ◽  
...  
Keyword(s):  
Cancer Detection ◽  
Pet Imaging ◽  
Imaging Study ◽  
Imaging Agents ◽  
Post Injection ◽  
Pyrimidine Derivatives ◽  
Micropet Imaging ◽  
Tumor Bearing ◽  
Biodistribution Data

Compound [18F]-8a exhibited good in vivo biodistribution data in mice bearing S180 tumor. And the microPET imaging study of [18F]-8a in S180 tumor-bearing mice was also preformed, which illustrated that the uptake in S180 tumor at 60 min post-injection of [18F]-8a was obvious.

scholarly journals 6-Deoxy-6-[131I]iodo-L-ascorbic Acid for the in Vivo Study of Ascorbate: Autoradiography, Biodistribution in Normal and Hypolipidemic Rats, and in Tumor-Bearing Nude Mice

2009 ◽  
Vol 32 (11) ◽  
pp. 1906-1911 ◽  
Author(s):  
Jintaek Kim ◽  
Fumihiko Yamamoto ◽  
Shigeki Gondo ◽  
Toshihiko Yanase ◽  
Takahiro Mukai ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Nude Mice ◽  
Tumor Bearing

scholarly journals Knockdown of microRNA-135b in Mammary Carcinoma by Targeted Nanodiamonds: Potentials and Pitfalls of In Vivo Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 866 ◽  
Author(s):  
Romana Křivohlavá ◽  
Eva Neuhӧferová ◽  
Katrine Q. Jakobsen ◽  
Veronika Benson
Keyword(s):  
Antisense Rna ◽  
Tumor Tissue ◽  
Immune Cell ◽  
Ex Vivo ◽  
Tumor Bearing ◽  
Intravenous Application ◽  
Effective Inhibition ◽  
Tumor Accumulation ◽  
Safe Approach

Nanodiamonds (ND) serve as RNA carriers with potential for in vivo application. ND coatings and their administration strategy significantly change their fate, toxicity, and effectivity within a multicellular system. Our goal was to develop multiple ND coating for effective RNA delivery in vivo. Our final complex (NDA135b) consisted of ND, polymer, antisense RNA, and transferrin. We aimed (i) to assess if a tumor-specific coating promotes NDA135b tumor accumulation and effective inhibition of oncogenic microRNA-135b and (ii) to outline off-targets and immune cell interactions. First, we tested NDA135b toxicity and effectivity in tumorospheres co-cultured with immune cells ex vivo. We found NDA135b to target tumor cells, but it binds also to granulocytes. Then, we followed with NDA135b intravenous and intratumoral applications in tumor-bearing animals in vivo. Application of NDA135b in vivo led to the effective knockdown of microRNA-135b in tumor tissue regardless administration. Only intravenous application resulted in NDA135b circulation in peripheral blood and urine and the decreased granularity of splenocytes. Our data show that localized intratumoral application of NDA135b represents a suitable and safe approach for in vivo application of nanodiamond-based constructs. Systemic intravenous application led to an interaction of NDA135b with bio-interface, and needs further examination regarding its safety.

In vivo biodistribution of pancreatic-derived factor using 18F-labeled PANDER PET imaging

2014 ◽  
Vol 301 (2) ◽  
pp. 333-339 ◽  
Author(s):  
Feng Hua Lai ◽  
Gang Hua Tang ◽  
Chi Jiao Yang ◽  
Hong Liang Wang ◽  
Kong Zhen Hu ◽  
...  
Keyword(s):  
Pet Imaging ◽  
In Vivo Biodistribution

Dependence of Humoral Hypercalcemia of Malignancy on Parathyroid Hormone-related Protein Expression in the Canine Anal Sac Apocrine Gland Adenocarcinoma (CAC-8) Nude Mouse Model

1998 ◽  
Vol 35 (5) ◽  
pp. 344-351 ◽  
Author(s):  
A. Gröne ◽  
M. T. Weckmann ◽  
E. A. G. Blomme ◽  
C. C. Capen ◽  
T. J. Rosol
Keyword(s):  
Nude Mice ◽  
Messenger Rna ◽  
Calcium Concentration ◽  
Humoral Hypercalcemia Of Malignancy ◽  
Serum Calcium Concentration ◽  
Tumor Bearing ◽  
Humoral Hypercalcemia ◽  
Parathyroid Hormone Related Protein ◽  
Anal Sac

Circulating parathyroid hormone-related protein (PTHrP) is the primary humoral factor in dogs with spontaneous humoral hypercalcemia of malignancy (HHM) and adenocarcinomas derived from apocrine glands of the anal sac. A canine apocrine adenocarcinoma model of HHM in nude mice (CAC-8) was developed and characterized. After 32 passages in vivo, a spontaneous variant of the tumor (CAC-8 Lo Ca) that has altered cellular morphology and that fails to induce HHM in tumor-bearing nude mice has been discovered. The hypercalcemic and nonhypercalcemic tumor lines were compared by tumor weight, effect on body weight, serum calcium concentration, plasma PTHrP concentration, histopathology, expression of PTHrP protein by radioimmunoassay and immunohistochemistry, and expression of PTHrP mRNA by in situ hybridization and northern blot analysis. Messenger RNA expression for other factors and cytokines known to alter PTHrP secretion or bone resorption in vivo, including tumor necrosis factor α (TNFα), interleukin (IL)-1, IL-6, and transforming growth factor β (TGFβ), were also measured in the adenocarcinomas. There was no significant difference in weight of individual tumors. Nude mice bearing the CAC-8 (Lo Ca) tumor maintained normal body weight as compared with non-tumor-bearing control mice. In contrast, mice with the CAC-8 (Hi Ca) tumor had markedly decreased body weights. The CAC-8 (Hi Ca) tumor-bearing mice had severe hypercalcemia (x~ = 13.4 mg/dl) and increased plasma concentrations of PTHrP (30.4 pM), whereas the CAC-8 (Lo Ca) tumor-bearing mice had a mean serum calcium concentration of 10.1 mg/dl and mildly increased PTHrP concentrations (5.7 pM) as compared with control mice (9.0 mg/dl and 1.0 pM, respectively). The original tumor (CAC-8 [Hi Ca]) is a well-differentiated adenocarcinoma, whereas the variant tumor (CAC-8 [Lo Ca]) is a solid carcinoma with both polygonal and spindle-shaped cells. The CAC-8 (Lo Ca) tumor had decreased PTHrP mRNA expression and protein synthesis. Messenger RNA expression of TGFβ, TNFα, IL-1, and IL-6 was similar in both tumors and was consistent with the central role of PTHrP in the induction of hypercalcemia in this animal model.

scholarly journals A versatile method for the selective core-crosslinking of hyaluronic acid nanogels via ketone-hydrazide chemistry: from chemical characterization to in vivo biodistribution

2018 ◽  
Vol 6 (7) ◽  
pp. 1754-1763 ◽  
Author(s):  
Francielle Pelegrin Garcia ◽  
Marlène Rippe ◽  
Mychelle V. P. Companhoni ◽  
Talitha Fernandes Stefanello ◽  
Benoit Louage ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Blood Circulation ◽  
Chemical Characterization ◽  
Circulation Time ◽  
In Vivo Biodistribution ◽  
Tumor Accumulation

Nanogels show long in vivo blood circulation time and high tumor accumulation.

scholarly journals Enhanced Anti-Tumor Effect of Folate-Targeted FA-AMA-hyd-DOX Conjugate in a Xenograft Model of Human Breast Cancer

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7110
Author(s):  
Tian-tian Liao ◽  
Jiang-fan Han ◽  
Fei-yue Zhang ◽  
Ren Na ◽  
Wei-liang Ye
Keyword(s):  
Breast Cancer ◽  
Nude Mice ◽  
Folate Receptor ◽  
Xenograft Model ◽  
Maximum Tolerated Dose ◽  
Therapeutic Drug ◽  
Antitumor Effects ◽  
Tumor Bearing

Folate-aminocaproic acid-doxorubicin (FA-AMA-hyd-DOX) was firstly synthesized by our group. It was indicated that FA-AMA-hyd-DOX was pH-responsive, and had strong cytotoxicity on a folate receptor overexpressing cell line (KB cells) in vitro. The aim of our study was to further explore the potential use of FA-AMA-hyd-DOX as a new therapeutic drug for breast cancer. The cellular uptake and the antiproliferative activity of the FA-AMA-hyd-DOX in MDA-MB-231 cells were measured. Compared with DOX, FA-AMA-hyd-DOX exhibited higher targeting ability and cytotoxicity to FR-positive tumor cells. Subsequently, the tissue distribution of FA-AMA-hyd-DOX was studied, and the result confirmed that DOX modified by FA can effectively increase the selectivity of drugs in vivo. After determining the maximum tolerated dose (MTD) of FA-AMA-hyd-DOX in MDA-MB-231 tumor-bearing nude mice, the antitumor effects and the in vivo safety of FA-AMA-hyd-DOX were systematically evaluated. The data showed that FA-AMA-hyd-DOX could effectively increase the dose of DOX tolerated by tumor-bearing nude mice and significantly inhibit MDA-MB-231 tumor growth in vivo. Furthermore, FA-AMA-hyd-DOX treatment resulted in almost no obvious damage to the mice. All the positive data suggest that FA-targeted FA-AMA-hyd-DOX is a promising tumor-targeted compound for breast cancer therapy.

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