scholarly journals Detection of Brucella antibodies of sheep and goats by using two serological tests in Al-Sulaimanya governorate

2015 ◽  
Vol 39 (2) ◽  
pp. 32-37
Author(s):  
Osman M. Jabary
Keyword(s):  
Positive Result ◽  
Rose Bengal ◽  
Whole Blood ◽  
Indirect Elisa ◽  
Serological Tests ◽  
Blood Samples ◽  
Sheep And Goats ◽  
Rose Bengal Test ◽  
Different Ages ◽  
Whole Blood Samples

     A research was carried out to detect Brucella antibodies of sheep and goats in Al-Sulaimanya governorate by using modified Rose Bengal test and indirect ELISA. A total of three hundred and eleven (311) whole blood samples (160 sheep and 151 goats) were collected randomly from eight different regions in Al-Sulaimanya governorate from unvaccinated flock with different ages. A total percentages of positive result by using modified Rose Bengal test was 14.46% (20% in sheep and 8.6% in goats), while by using indirect ELISA was 27.6% (35.2% in sheep and 19.2% in goats) with significant (P<0.05) differences. It revealed that rates to modified Rose Bengal test were 14.34% in female and 10.09% in males while to indirect ELISA 26.35% in female and 33.9% in males in sheep and goats, In conclusion of this study the high seroprevelence was at 1-3 years 19.2% and ˃3 years 33.96% according to modified Rose Bengal test and indirect ELISA, respectively. 

scholarly journals Detection of Brucella Antibodies of Sheep in Al-Anbar Province by Using Some Serological Tests

2013 ◽  
Vol 37 (1) ◽  
pp. 7-12
Author(s):  
Ekram A. Al-Samarrae
Keyword(s):  
Rose Bengal ◽  
Agglutination Test ◽  
Infected Sheep ◽  
Serological Tests ◽  
Blood Samples ◽  
Plate Test ◽  
Sheep Blood ◽  
Total Percentage ◽  
Different Ages

An investigation was carried out to detect anti-Brucella antibodies of sheep in Al-Anbar province by using few serological tests; rose Bengal plate test (RBPT) and tube agglutination test (TAT). A total of 754 sheep blood samples (94 Ram and 660 Ewe) were collected randomly distributed in Al-Anbar province from unvaccinated sheep flocks with different ages. A total percentage of infected sheep depending on RBPT was 10.21% with significant differences at (P< 0.01), it was 9.69% in ewes, whereas in rams it elevated to reach 13.82%, in addition to the disease was higher seroprevalence at > 3 years (12.78%) in both gender with significant differences at (P<0.05) than lower ages. Out of 77 RBPT positive sera, 38(49.35%) were positive using TAT, there was 14 seropositive with significant differences at (P< 0.01). The study reported that brucellosis was distributed in Al-Anbar province with variable percent (3.26% to 21.27%), as well as emphasized on different epidemiological aspects.

Steadiness/stability of antiepileptic drugs in serum, plasma and whole-blood samples under various storage methods

2010 ◽  
Vol 41 (02) ◽  
Author(s):  
N Shazi ◽  
A Böss ◽  
HJ Merkel ◽  
F Scharbert ◽  
D Hannak ◽  
...  
Keyword(s):  
Antiepileptic Drugs ◽  
Whole Blood ◽  
Blood Samples ◽  
Storage Methods ◽  
Whole Blood Samples

scholarly journals Determination of 19 Psychoactive Substances in Premortem and Postmortem Whole Blood Samples Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Separations ◽  
2021 ◽  
Vol 8 (6) ◽  
pp. 78
Author(s):  
Sevasti Karampela ◽  
Jessica Smith ◽  
Irene Panderi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Formic Acid ◽  
Whole Blood ◽  
High Performance ◽  
Tandem Mass ◽  
Psychoactive Substances ◽  
Blood Samples ◽  
Whole Blood Samples

An ever-increasing need exists within the forensic laboratories to develop analytical processes for the qualitative and quantitative determination of a broad spectrum of new psychoactive substances. Phenylethylamine derivatives are among the major classes of psychoactive substances available on the global market and include both amphetamine analogues and synthetic cathinones. In this work, an ultra-high-performance liquid chromatography-positive ion electrospray ionization tandem mass spectrometric method (UHPLC-ESI-MS/MS) has been developed and fully validated for the determination of 19 psychoactive substances, including nine amphetamine-type stimulants and 10 synthetic cathinone derivatives, in premortem and postmortem whole blood. The assay was based on the use of 1 mL premortem or postmortem whole blood, following solid phase extraction prior to the analysis. The separation was achieved on a Poroshell 120 EC-C18 analytical column with a gradient mobile phase of 0.1% formic acid in acetonitrile and 0.1% formic acid in water in 9 min. The dynamic multiple reaction monitoring used in this work allowed for limit of detection (LOD) and lower limit of quantitation (LOQ) values of 0.5 and 2 ng mL−1, respectively, for all analytes both in premortem and postmortem whole blood samples. A quadratic calibration model was used for the 12 quantitative analytes over the concentration range of 20–2000 ng mL−1, and the method was shown to be precise and accurate both in premortem and postmortem whole blood. The method was applied to the analysis of real cases and proved to be a valuable tool in forensic and clinical toxicology.

scholarly journals Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples

2021 ◽  
Vol 2 (1) ◽  
pp. 100311
Author(s):  
Daniella C. Terenzi ◽  
Ehab Bakbak ◽  
Justin Z. Trac ◽  
Mohammad Al-Omran ◽  
Adrian Quan ◽  
...  
Keyword(s):  
Whole Blood ◽  
Progenitor Cell ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Isolation And Characterization ◽  
Whole Blood Samples

Detection of melanoma cells in whole blood samples using spectral imaging and optical clearing

2020 ◽  
pp. 1-1
Author(s):  
Polina A. Dyachenko Timoshina ◽  
Leonid E. Dolotov ◽  
Ekaterina N. Lazareva ◽  
Anastasiia A. Kozlova ◽  
Olga A. Inozemtseva ◽  
...  
Keyword(s):  
Whole Blood ◽  
Spectral Imaging ◽  
Melanoma Cells ◽  
Blood Samples ◽  
Optical Clearing ◽  
Whole Blood Samples

The distribution of BrKα/RbKα peak intensity ratio in human whole blood samples

1994 ◽  
Vol 42 (3) ◽  
pp. 231-241 ◽  
Author(s):  
C. Shenberg ◽  
S. Spiegel ◽  
S. Chaitchik ◽  
P. Jordan ◽  
M. Kitzis ◽  
...  
Keyword(s):  
Whole Blood ◽  
Intensity Ratio ◽  
Peak Intensity Ratio ◽  
Blood Samples ◽  
Human Whole Blood ◽  
Peak Intensity ◽  
Whole Blood Samples
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