scholarly journals The Effects of Temperature, Light and Moisture on the Seed Germination of Siphonostegia chinensis Benth.

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Yongji Wang
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Germination Index ◽  
High Concentration ◽  
Complete Darkness ◽  
Germination Potential ◽  
Medium Level ◽  
Different Temperatures ◽  
High Level ◽  
Germination Experiment

To explore the optimum temperature, light intensityand water conditions for seed germination of Siphonostegia chinensis Benth.,seed germination experiment were carried out under different temperatures（5/15, 10/20, 15/25, 20/30℃）, different light intensity（14h light/10h darkness, complete darkness）and different concentrations（0％, 5％, 10％, 15％, 20％）of PEG-6000 solution. In terms of concentration, 5% PEG was regarded as the low level, 10% and 15% as the medium level, and 20% as the high level. The results showed that (1) Germination rate, germination potential, and germination index were increased with the rise of temperature. In addition, seed germination was significantly higher under the dark conditions than that with the 14h light/10h darkness. (2) No seed germination occurred when the temperature was below 10/20 ºC at 14h light/10h darkness. (3) Under 14h light/10h darkness, the germination rate, germination potential and germination index first increased and then decreased with the increase of PEG concentration. The low concentration was more beneficial to the seed germination. (4) Under the condition of complete darkness, the germination rate, germination potential and germination index decline with fluctuation with the increase of PEG concentration. Seed germination of Siphonostegia chinensis Benth. was inhibited in high concentration of PEG.

scholarly journals Effects of Temperature on Seed Germination of Invasive Plant Rorippa amphbia (L.) Besser

2019 ◽  
pp. 1-7
Author(s):  
Yufeng Xu ◽  
Miao Liu ◽  
Yuanhao Lv ◽  
Qiuli Wang ◽  
Bo Qu ◽  
...  
Keyword(s):  
Global Warming ◽  
Seed Germination ◽  
Filter Paper ◽  
Invasive Plant ◽  
Germination Rate ◽  
Petri Dish ◽  
Liaoning Province ◽  
Germination Index ◽  
Temperature Range ◽  
Germination Potential

Aims: Global warming and biological invasion are major environmental issues faced in the world. In the study, Rorippa amphibia, a perennial invasive clone plant in northern China, was used as a material to study the germination characteristics of the seeds at different temperatures. Study Design: Germination test of R. amphibia seeds at different temperature was studied by means of laboratory culture. The germination percentage, germination index, germination potential, bud height and root length of the seeds were determined. Place and Duration of Study: Samples were collected from the west side of the swimming pool of Shenyang Agricultural University of Liaoning Province in August 2017. Experiments were done in the College of Biological Science and Technology, between October 2017 and June 2018. Methodology: The petri dish method was used in the experiment. Fifty seeds were randomly selected and soaked in distilled water for 12h. The seeds were placed in a petri dish covered with double filter paper, cultured at 10℃, 15℃, 20℃, 25℃, 30℃, 35℃ and 40℃ in light incubators for 12h darkness and 12h light (4000lux), with 3 repeats per processing. Seed germination was based on embryo root breakthrough seed coat ≥ 1 mm. During the experiment, the numbers of seed germination were recorded every day, and the filter paper was kept moist until there was no new seed germination for 2 consecutive days, which was regarded as the end of germination. The numbers of seed germination should be counted regularly every day, and the beginning and duration of germination should be recorded. The germination rate, daily germination rate, germinating potential, germinating index and vigor index of R. amphibia seeds at different temperature were calculated by measuring bud height and radicle length on the 10th day after germination. Results: The temperature range of seed germination of R. amphibia was wide, which could germinate at 15 - 40℃. Lower temperature delayed the peak period of seed germination at some extent and the germination rates of R. amphibia peak at 30 - 35℃, which were 44.67% and 50% respectively. At 35℃, germination potential and germination index were 25.33% and 29.46, reaching the maximum value. Conclusion: The reason for the wide temperature range of seed germination and the low germination rate might be the candidate method for clonal plant population establishment in temperate zone. The higher germination rate of high temperature condition suggested that clone invasive plants in temperate regions were more invasive during global warming.

Effects of Biogas Slurry Pretreatment on Germination and Seedling Growth of Vicia faba L.

2014 ◽  
Vol 955-959 ◽  
pp. 208-212 ◽  
Author(s):  
Yan Zhao ◽  
Qing Song Yang ◽  
Shu Yang ◽  
Hong Mei Zhao ◽  
Qing Song Duan ◽  
...  
Keyword(s):  
Seed Germination ◽  
Vicia Faba ◽  
Seedling Growth ◽  
Germination Rate ◽  
Germination Index ◽  
Biogas Slurry ◽  
Seed Soaking ◽  
Germination Potential ◽  
Faba Beans ◽  
Germination And Growth

In order to study the effect of seed soaking with biogas slurry on seed germination and growth of faba beans (Vicia faba L.), the experiment was conducted. The seeds were soaked in biogas slurry for various soaking durations (0, 2, 4, 6 and 8 hours) and different concentration (0, 25, 50, 75 and 100 %) along with control to determine the optimal soaking duration and find out the best growing condition. The highest germination rate, germination potential and germination index were obtained when the faba beans seeds soaked for 4 hours in 75 % biogas slurry. Seedling growth was recorded in plants where seeds soaked for 6 hours in 75 % biogas slurry. It was concluded that the seed soaking of biogas slurry with lower concentration (25, 50 and 75 %) showed best results regarding maximum germination and enhanced growth. However, under the higher concentration (100 %) conditions of biogas slurry had a tendency of decrease on seed germination and seedling growth.

Influence of Heat on Seed Germination of Cistus Laurifolius and Cistus Ladanifer

1992 ◽  
Vol 2 (1) ◽  
pp. 15 ◽  
Author(s):  
L Valbuena ◽  
R Tarrega ◽  
E Luis
Keyword(s):  
Seed Germination ◽  
High Temperatures ◽  
Exposure Time ◽  
Germination Rate ◽  
Heat Exposure ◽  
Cistus Ladanifer ◽  
Different Temperatures ◽  
Petri Dishes ◽  
Germinated Seeds

The influence of high temperatures on germination of Cistus laurifolius and Cistus ladanifer seeds was analyzed. Seeds were subjected to different temperatures for different times, afterwards they were sowed in plastic petri dishes and monitored for germinated seeds over two months.The germination rate observed in Cistus ldanifer was greater than in Cistus laurifolius. In both species, heat increased germination percentages. For Cistus laurifolius higher temperatures or longer exposure times were needed. Germination percentages of Cistus ladanifer were lower when heat exposure time was 15 minutes.It must be emphasized that germination occurred when seeds were not treated, while seeds exposed to 150�C for 5 minutes or more did not germinate.

Temperature Effect on Seed Germination Performance of a Local Variety of Onion in Bangladesh

2021 ◽  
Vol 1 (01) ◽  
pp. 27-30
Author(s):  
IRANI KHATUN ◽  
RIYAD HOSSEN
Keyword(s):  
Seed Germination ◽  
Temperature Effect ◽  
Performance Test ◽  
Germination Rate ◽  
Germination Percentage ◽  
Optimum Range ◽  
Local Variety ◽  
Different Temperatures ◽  
Local Farmers

Seed germination performance test of Taherpuri onion (a local variety of onion) under six different temperatures (15, 20, 25, 30, 35 and 40°C) was the main goal of this experiment. Germination percentage (GP) was calculated at highest 60.25% at 25°C, and the highest germination rate 20.08 was observed in the same temperature condition. The lowest germination performance (13.25 % germi-nation and 3.32 seeds per day as germination rate) was found at 40°C temperature. Finally, the authors mentioned the temperature 20 to 30°C as optimum range, and suggested the temperature 25°C as best suited for obtaining highest results in case of both germination percentage and germination rate of these seeds. To produce maximum seedlings of the local variety of onion, the mentioned temperature should be followed by the local farmers.

scholarly journals Acid and Temperature Treatments Result in Increased Germination of Seeds of Three Fescue Species

2012 ◽  
Vol 40 (2) ◽  
pp. 220 ◽  
Author(s):  
Rade S. STANISAVLJEVIC ◽  
Savo M. VUCKOVIC ◽  
Aleksandar S. SIMIC ◽  
Jordan P. MARKOVIC ◽  
Zelijco P. LAKIC ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Seed Germination ◽  
Acid Solution ◽  
Sulfuric Acid Solution ◽  
Germination Rate ◽  
Festuca Rubra ◽  
Time Intervals ◽  
Different Temperatures ◽  
Successful Establishment ◽  
Seed Collecting

Efficient germination of fescue seeds is essential for successful establishment of meadows and pastures. This research was conducted to ascertain the effects of various acid and temperature treatments on seed germination in three fescue species: Festuca rubra, F. ovina, and F. pratensis. Seeds from different cultivars, populations, or lots were exposed either to four concentrations of sulfuric acid at three different time intervals (12 treatments) or six different temperatures at three different time intervals (18 treatments). Despite all belonging to the genus Festuca, the seed from different species responded differently to the treatments. The three optimum treatments for F. rubra seed involved soaking in a 75% solution of sulfuric acid for 20 minutes (improved the germination rate by 19%), soaking in a 50% solution of sulfuric acid for 30 minutes (improved the germination rate by 18%) and exposure to either 60°C or 70°C for 90 minutes (improved the germination rate by 17%). For F. ovina seed, optimal treatments included soaking seeds for either 10 or 20 minutes in a 50% sulfuric acid solution (both treatments improved germination rates by 13%) or exposing seeds for 30 minutes in a 25% sulfuric acid solution and 80°C for 60 minutes (improved germination rate by 12%). Two optimal treatments were identified for F. pratensis seed. Whereas the first involved soaking the seeds in a 75% sulfuric acid solution for 30 minutes (improved germination rates by 22%), the second involved either exposing the seeds to 90°C for 90 or 60 minutes, or exposing the seeds to 80°C for 90 minutes (improved germination rate by 21%). Our findings indicate that if fescue seed is to be sown during the autumn (two to three months after seed collecting), treating it with acid and temperature can significantly enhance its germination.

Effect of Composting, Leaching and Carbonizing on Alleviating Phytotoxicity of Sewage Sludge

2012 ◽  
Vol 518-523 ◽  
pp. 1733-1736
Author(s):  
Hong Cai ◽  
Yi Bo Wen ◽  
Ji Li
Keyword(s):  
Seed Germination ◽  
Sewage Sludge ◽  
Adverse Effects ◽  
Root Length ◽  
Germination Rate ◽  
Germination Index ◽  
Pretreatment Method

Sewage sludge (SS) contains deleterious constituents that strongly inhibited seed germination. Pretreatment methods of composting, leaching and carbonizing were thus evaluated to alleviate the phytotoxicity. All the pretreatments improved germination rate, root length and germination index of radish seeds. The best performances were presented by composting pretreatment and the pretreatment of carbonizing plus leaching. Composting greatly alleviated phytotoxicity of SS and improved seed germination, however, it also showed stunted root length and germination index compared with the soil control. The adverse effects of composted SS on seed germination were completely eliminated by leaching, while carbonizing had little effect on reversing the stunt. These results indicated that the most effective pretreatment method to alleviate phytotoxicity of SS were composting plus leaching.

scholarly journals Non-Deep Physiological Dormancy in Seed and Germination Requirements of Lysimachia coreana Nakai

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 490
Author(s):  
Saeng Geul Baek ◽  
Jin Hyun Im ◽  
Myeong Ja Kwak ◽  
Cho Hee Park ◽  
Mi Hyun Lee ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Germination Rate ◽  
Seed Viability ◽  
Cold Stratification ◽  
Dormancy Breaking ◽  
Viability Test ◽  
Physiological Dormancy ◽  
Different Temperatures ◽  
Germination Requirements

This study aimed to determine the type of seed dormancy and to identify a suitable method of dormancy-breaking for an efficient seed viability test of Lysimachia coreana Nakai. To confirm the effect of gibberellic acid (GA3) on seed germination at different temperatures, germination tests were conducted at 5, 15, 20, 25, 20/10, and 25/15 °C (12/12 h, light/dark), using 1% agar with 100, 250, and 500 mg·L−1 GA3. Seeds were also stratified at 5 and 25/15 °C for 6 and 9 weeks, respectively, and then germinated at the same temperature. Seeds treated with GA3 demonstrated an increased germination rate (GR) at all temperatures except 5 °C. The highest GR was 82.0% at 25/15 °C and 250 mg·L−1 GA3 (4.8 times higher than the control (14.0%)). Additionally, GR increased after cold stratification, whereas seeds did not germinate after warm stratification at all temperatures. After cold stratification, the highest GR was 56.0% at 25/15 °C, which was lower than the GR observed after GA3 treatment. We hypothesized that L. coreana seeds have a non-deep physiological dormancy and concluded that 250 mg·L−1 GA3 treatment is more effective than cold stratification (9 weeks) for L. coreana seed-dormancy-breaking.

scholarly journals Symbiotic and Asymbiotic Germination of Dendrobium officinale (Orchidaceae) Respond Differently to Exogenous Gibberellins

2020 ◽  
Vol 21 (17) ◽  
pp. 6104 ◽  
Author(s):  
Juan Chen ◽  
Bo Yan ◽  
Yanjing Tang ◽  
Yongmei Xing ◽  
Yang Li ◽  
...  
Keyword(s):  
Seed Germination ◽  
Mycorrhizal Fungi ◽  
Calcium Binding ◽  
Germination Rate ◽  
Dendrobium Officinale ◽  
Fungal Colonization ◽  
Fungal Mycelium ◽  
High Concentration ◽  
Symbiotic Germination ◽  
Asymbiotic Germination

Seeds of almost all orchids depend on mycorrhizal fungi to induce their germination in the wild. The regulation of this symbiotic germination of orchid seeds involves complex crosstalk interactions between mycorrhizal establishment and the germination process. The aim of this study was to investigate the effect of gibberellins (GAs) on the symbiotic germination of Dendrobium officinale seeds and its functioning in the mutualistic interaction between orchid species and their mycobionts. To do this, we used liquid chromatograph-mass spectrometer to quantify endogenous hormones across different development stages between symbiotic and asymbiotic germination of D. officinale, as well as real-time quantitative PCR to investigate gene expression levels during seed germination under the different treatment concentrations of exogenous gibberellic acids (GA3). Our results showed that the level of endogenous GA3 was not significantly different between the asymbiotic and symbiotic germination groups, but the ratio of GA3 and abscisic acids (ABA) was significantly higher during symbiotic germination than asymbiotic germination. Exogenous GA3 treatment showed that a high concentration of GA3 could inhibit fungal colonization in the embryo cell and decrease the seed germination rate, but did not significantly affect asymbiotic germination or the growth of the free-living fungal mycelium. The expression of genes involved in the common symbiotic pathway (e.g., calcium-binding protein and calcium-dependent protein kinase) responded to the changed concentrations of exogenous GA3. Taken together, our results demonstrate that GA3 is probably a key signal molecule for crosstalk between the seed germination pathway and mycorrhiza symbiosis during the orchid seed symbiotic germination.

Effects of environmental factors on germination and emergence ofAmaranthus retroflexus

Weed Science ◽  
1999 ◽  
Vol 47 (5) ◽  
pp. 505-510 ◽  
Author(s):  
R. Ghorbani ◽  
W. Seel ◽  
C. Leifert
Keyword(s):  
Seed Germination ◽  
Control Strategies ◽  
Germination Rate ◽  
Soil Surface ◽  
Dry Weight ◽  
Amaranthus Retroflexus ◽  
Nitrogen Supply ◽  
Biological Weed Control ◽  
Detailed Knowledge ◽  
Different Temperatures

Detailed knowledge about the environmental conditions required for weed seed germination and establishment in soil is an important prerequisite for the development of integrated and biological weed control strategies. Germination and establishmentof Amaranthus retroflexuswere studied at different temperatures, planting depths, soil types, nitrogen supply, and water potentials. The minimum temperature for seed germination was > 5 C; maximum germination occurred between 35 and 40 C. At temperatures between 25 and 35 C, an additive effect on germination rate was observed when temperature and water availability were increased. For all soils tested, the percentage emergence of seeds placed on the soil surface and 4 cm deep was significantly lower than seeds placed between 0.5 and 3 cm. Emergence in the four sandy soils was generally greater than in the two heavier soils included in the study. There was a highly significant interaction between seed depth and soil type. Plant growth was also greatest in the lighter soils. Plant height, number of leaves, leaf area, fresh and dry weight, and nitrogen and carbon percentage in plant tissues ofA. retroflexusincreased significantly with increasing soil nitrogen supply.

Effect of light, salinity, and temperature on seed germination of Limonium stocksii

2004 ◽  
Vol 82 (2) ◽  
pp. 151-157 ◽  
Author(s):  
Sabahat Zia ◽  
M Ajmal Khan
Keyword(s):  
Seed Germination ◽  
Temperature Regime ◽  
Germination Rate ◽  
Gradual Decrease ◽  
Salinity Treatment ◽  
Optimal Temperature ◽  
Complete Darkness ◽  
Temperature Regimes ◽  
Effect Of Light ◽  
Recovery Temperature

Limonium stocksii (Boiss.) Kuntze (Plumbaginaceae) is a perennial, woody shrub distributed at Hawks Bay, Karachi, Pakistan. Experiments were carried out to investigate seed germination responses of L. stocksii at different salinities (0, 100, 200, 300, 400, and 500 mmol/L NaCl) and under different temperature regimes (10:20, 15:25, 20:30, and 25:35 °C), both in a 12 h dark : 12 h light photoperiod and in complete darkness. The highest percentage of germination (about 100%) was obtained at 0, 100, and 200 mmol/L NaCl at 20:30 °C, and a further increase in salinity resulted in a gradual decrease in germination. Less than 5% of seeds germinated at 500 mmol/L NaCl. Germination under salinity treatment at 15:25 °C was slightly more inhibitory than the optimal temperature regime, whereas under both 10:20 and 25:35 °C temperature regimes, seed germination was substantially reduced and few seeds germinated at concentrations higher than 200 mmol/L NaCl. Germination rate was fastest at 20:30 °C and slowest at 10:20 °C. Relatively low seed germination was obtained in the dark in comparison to seeds germinated in a 12-h photoperiod under saline conditions. Recovery experiments showed that exposure of seeds to various salinity and temperature regimes had little effect on viability of seeds.Key words: germination, light, Limonium stocksii, NaCl, recovery, temperature.

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