scholarly journals Gas chromatography-Mass spectrometry (GC-MS) analysis of bioactive components present in grape citrus peel in Nigeria

2021 ◽  
Vol 8 (1) ◽  
pp. 166-174
Author(s):  
Israel Chukwuemeka Eneke ◽  
Eka Bassey Essien ◽  
Matthew Owhonda Wegu
Keyword(s):  
Retention Time ◽  
Research Work ◽  
Value Added ◽  
Gas Chromatography Mass Spectrometry ◽  
Citrus Paradisi ◽  
Bioactive Components ◽  
Citrus Peel ◽  
Mass Selective Detector ◽  
Ms Analysis ◽  
Grape Peel

Grapefruit (Citrus paradisi) is an important cultivar of the Citrus genus which contains a number of nutrients beneficial to human health. Grapefruit peels are usually thrown away in this part of Nigeria. The research work investigated the bioactive components present in an indigenous citrus peel, grape (Citrus paradisi). Grape fruits were purchased from the fruits garden market in D-Line, Port-Harcourt metropolis and washed with ionized water and allowed to shade dry. The peel of the fruits were separated and subjected to cold extraction using 95% ethanol. The extracts obtained were further extracted in dichloromethane and subjected to GC/MS analysis for characterization of various bioactive components. The gas chromatographic model: 789A (GC) analysis was performed on an agilent technologies interfaced with mass selective detector model: 5975(MSD). The results revealed 25 bioactive components in grape peel with n-Hexadecanoic acid showing the highest concentration of 20.36% and retention time of 18.522min. Nootkatone was the lowest component in the grape peel with concentration 0.74% and retention time of 16.459min. Results shows that grapefruit (Citrus paradisi) has considerable potential as a source of natural bioactive components with different retention times. These fruits residues which otherwise regarded as waste hold promising potentials for medicinal therapy and value added food supplements.

scholarly journals GC-MS ANALYSIS OF METHANOLIC STEM EXTRACT OF GYNOCHTHODES RIDSDALEI, RAZAFIM AND B. BREMER, AN ENDEMIC, ENDANGERED MEDICINAL PLANT OF SOUTHERN WESTERN GHATS

2017 ◽  
Vol 9 (3) ◽  
pp. 98
Author(s):  
Renji R. Nair ◽  
A. Gangaprasad
Keyword(s):  
Retention Time ◽  
Mass Spectra ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Bioactive Components ◽  
Endangered Medicinal Plant ◽  
Southern Western Ghats ◽  
Stem Extract ◽  
Weight Structure ◽  
Kerala State

Objective: The present research study was undertaken to determine the presence of bioactive components present in the methanolic stem extract of Gynochthodes ridsdalei using Gas Chromatography–Mass Spectrometry (GC-MS) analysis.Methods: the Fresh stem of Gynochthodes ridsdalei collected from the forest areas of Ponmudi region of Thiruvananthapuram district of Kerala state, India was used. The mass spectrum GC-MS of the crude methanolic extract was estimated using the database of National Institute of Standard and Technology (NIST).Results: The active principles with their retention time, peak area, molecular formula, molecular weight, structure and category of the compound were predicted. The analysis revealed the presence of 52 bioactive components. Most of the identified compounds are basically biological important. The components were identified by comparing their retention time and peak area with that of literature and by interpretation of mass spectra. The phyto components screened were of biological importance. Some of them were sterols, anthraquinones, vitamins etcConclusion: The result reveals the existence of various bioactive compounds and validates the earlier reports of therapeutic importance of the plant. Gynochthodes ridsdalei is recommended as a plant of phytochemical and pharmaceutical importance

GC-MS Analysis and Molecular Docking of Bioactive Components from Leaves of Guaiacum officinale for Anti-inflammatory Activity

2019 ◽  
Vol 4 (3) ◽  
pp. 180-184
Author(s):  
Vijetha Pendyala ◽  
Vidyadhara Suryadevara ◽  
Subbarao Jampani ◽  
Suhasini Jala
Keyword(s):  
Molecular Docking ◽  
Phospholipase A2 ◽  
Retention Time ◽  
Docking Studies ◽  
Bioactive Components ◽  
Molecular Docking Studies ◽  
Ms Analysis ◽  
Cox 2 ◽  
Guaiacum Officinale ◽  
Interleukin Receptor

Present study was an attempt to investigate the bioactive components present in the leaves of Guaiacum officinale using gas chromatography-mass spectroscopy (GC-MS) analysis and study the antiinflammatory potential of those constituents using molecular docking studies. GC-MS analysis was done by standard protocol using the equipment JEOL GC MATE II. The identification of components was based on NIST (National Institute of Standards and Technology) Version-11 library as well as comparison of their retention indices. The molecular docking studies were done using the commercial docking software MCULE, 1-click docking. GC-MS analysis of the alcoholic extract showed the presence of ten compounds at different retention times. The phytoconstituent 8,11,14-eicosatrienoic acid was present at high concentration with % peak area of 43.3 at a retention time of 19.43 min followed by 5,7-dihydroxy-8-methoxy flavone (Wogonin) at a retention time of 17.73. All 10 compounds obtained from GC-MS analysis and diclofenac were used as the ligands in this study, with cyclooxygenase-2 (COX-2), phospholipase A2 and interleukin receptor as the molecular targets. in silico Docking studies revealed that the flavanoid Wogonin is having highest binding potential indicated by least docking score of -8.2, -8 and -6.9 kcal/mol on COX-2, phospholipase A2 and interleukin receptor respectively.

Py-GC/MS Analysis of Bioactive Components of 450°C Pyrolyzate from Ethanol Extractives of Oil-Tea Cake

2011 ◽  
Vol 480-481 ◽  
pp. 513-518
Author(s):  
Qi Mei Liu ◽  
Wan Xi Peng
Keyword(s):  
Phosphonic Acid ◽  
Value Added ◽  
Bioactive Components ◽  
Cyclobutanedicarboxylic Acid ◽  
Ms Analysis ◽  
Cosmetic Industry ◽  
Analytical Result ◽  
Main Components

450°C-based pyrolysis- GC/MS technology was used to analyze the bioactive components of ethanol extractives of oil-tea cake. The analytical result showed that 38 peaks were obtained from the 450°C-based pyrolyzate of ethanol extractives of oil-tea cake, and 37 compounds representing 97.81 % of the total areas were identified (Table 1). The analytical result showed that the main components of ethanol extractives of oil-tea cake by 450°C-based pyrolysis- GC/MS were as: Benzene, 1,2,3-Trimethoxy-5-Methyl- (21.56%), Phenol, 3,4-Dimethoxy- (16.31%), 3',5'-Dimethoxyacetophenone (14.37%), 1,2-Ethanediamine, N-Ethyl- (6.62%), Indole (5.39%), Mequinol (4.13%), Phosphonic Acid, (P-Hydroxyphenyl)- (2.97%), 1,2-Cyclobutanedicarboxylic Acid, Cis- (1.67%), Bicyclo 3.1.0 Hexan-3-One (1.38%), Benzene, 1,4-Dimethoxy-2,3,5,6- Tetramethyl- (1.35%), 2h-Azepin-2-One, Hexahydro-1-(2-Propenyl)- (1.15), etc. The results of function analyses showed that the 450°C-based pyrolyzate of ethanol extractives of oil-tea cake can be used as rare natural medicinal materials, and value-added materials of spice, food and cosmetic industry.

GC-MS Analysis of Antibacterial Phytochemicals from Cassia sophera Linn

2020 ◽  
Vol 13 (5) ◽  
pp. 5131-5137
Author(s):  
Dr. Shyamal K. Jash ◽  
Dilip Gorai ◽  
Lalan Chandra Mandal ◽  
Sekhar Pal
Keyword(s):  
Chemical Constituents ◽  
Research Work ◽  
Enterobacter Aerogenes ◽  
Gas Chromatography Mass Spectrometry ◽  
Gram Negative Bacteria ◽  
Research Groups ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Ms Analysis ◽  
The World

Cassia sophera Linn (Leguminosae) plants are widely distributed worldwide, and find immense applications in traditional systems of medicine in many countries. The plant has been partly explored by various research groups in the world. In our present study, two oils (OL-1 & OL-2) from aerial parts and roots of Cassia sophera have been evaluated by GC/MS using Perkin-Elmer Gas Chromatography–Mass Spectrometry and comparison of spectral data with the existing in National Institute of Standards and Technology (NIST) library. GC/MS analysis of the two oils revealed the presence of a number of chemical constituents. Again, the isolated oils exhibited considerable antibacterial activity against two Gram positive bacteria viz. Bacillus megaterium & Staphylococcus aureus and two Gram negative bacteria viz. Escherichia coli & Enterobacter aerogenes. Therefore, the present study will definitely inspire the researchers engaged in this direction to undertake further research work on this plant for searching new lead molecules

Enzymatic digestion, solid-phase extraction, and gas chromatography/mass spectrometry of derivatized intact oxazepam in urine

1991 ◽  
Vol 37 (9) ◽  
pp. 1595-1601 ◽  
Author(s):  
J G Langner ◽  
B K Gan ◽  
R H Liu ◽  
L D Baugh ◽  
P Chand ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Solid Phase ◽  
Internal Standard ◽  
Enzymatic Digestion ◽  
Gas Chromatography Mass Spectrometry ◽  
Extraction Recovery ◽  
Mass Selective Detector ◽  
Operation Conditions ◽  
Ms Analysis ◽  
Gas Chromatography Mass Spectroscopy

Abstract Enzymatic digestion with beta-glucuronidase (EC 3.2.1.31) was used to release intact oxazepam from urine samples containing the d5-analog internal standard. The resulting specimens were extracted with Du Pont PREP Type W cartridge (processed by a PREP Automated Sample Processor), Bond Elut Certify, and J.T. Baker "spe" columns for comparison of the columns' extraction recovery and overall effectiveness. Methyl iodide/tetrahexylammonium hydrogen sulfate and N,O-bis(trimethylsilyl)trifluoroacetamide/trimethylchlorosilane (10 g/L) were used for the methylation and trimethylsilylation studies. We used a Hewlett-Packard HP 5790 mass-selective detector equipped with a 13-m J & W DB-5 column (5% phenyl polysiloxane phase) for gas chromatography/mass spectroscopy (GC/MS) analysis and the Thru-Put Target software package for data processing. After several exploratory experiments, we adopted the Du Pont PREP system methylation procedure because of its effective recovery, the superior stability of the derivatization product, the possibility of incorporating a clean-up step, and the potential for high throughput. The extraction recovery from a set of control samples was 87%. Coefficients of variation obtained for six replicates of GC/MS analysis and for the overall procedure were 1% and 3%, respectively. Excellent linearity was established in the 50-8000 micrograms/L concentration range studied. With the use of 3-mL samples, a 20-microL final reconstitution volume, oxazepam at 50 micrograms/L was easily detected under the adopted operation conditions.

Determination of Bioactive and Pharmaceutical Components of Croton bonplandianus by GC-MS Analysis

1970 ◽  
Vol 9 (5) ◽  
pp. 3488-3493
Author(s):  
Selvakumar Sivagnanam, ◽  
Valliammai R
Keyword(s):  
Medicinal Plant ◽  
Retention Time ◽  
Mass Spectra ◽  
Mass Spectrometric ◽  
Drug Formulation ◽  
Bioactive Components ◽  
Indian Medicinal Plant ◽  
Aerial Parts ◽  
Ms Analysis

The present study sought to analyze the chloroformic extract of aerial parts of the Indian medicinal plant Croton bonplandianus by GC-MS analysis and to explore the presence of major phytoconstituents. Gas chromato-graphy–mass spectrometric (GC–MS) analysis was carried out on the chloroformic extract of aerial parts of Croton bonplandianus for identification of phytocomponents in the plant while the mass spectra of the components  found in the extract was matched with the National Institute of Standards and Technology (NIST) library. There are 59 bioactive components were identified from chloroformic extract of Croton bonplandianus. Hence, it is of interest to investigate the presence of phytoconstituents from the chloroformic extract of Croton bonplandianus by using GC-MS method. The highest peak area (%) of 21.710 was obtained by Phenol,2-methyl1-5-(1,2,2-trimethyl cyclo pentyl)-(s) (Retention time 28.236) and the lowest peak area (%) of 0.76 was obtained by Bicyclo [7,2,0] undecane,10,10-dimethyl1-2,6-bis (methylene)-[1S-1R*,9S*)] (Retention time 29.396).  Results confirmed the presence of therapeutically potent compounds in the plant extract. This study is helpful to predict the formula and structure of biomolecules which can be used as drugs and further investigation may lead to the development of drug formulation.

scholarly journals GC-MS analysis of bioactive compounds from Freshwater mussels of Parreysia corrugata (Muller 1774) and their pharmacological activities

2019 ◽  
Vol 9 (4-A) ◽  
pp. 155-158
Author(s):  
N. Santhiya ◽  
M. Ramasamy
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Retention Time ◽  
Mass Spectra ◽  
Freshwater Mussels ◽  
Volatile Matter ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Branched Chain

GC-MS is one of the best techniques to identify the constituents of volatile matter, long chain, branched chain hydrocarbons, alcohols acids, esters etc. The freshwater mussels Parreysia corrugata was analyzed using Gas Chromatography–Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. Gas chromatography mass spectrometry (GC-MS) analysis revealed the presence of 26 compounds. The compounds were identified by comparing their retention time and peak area with that of literature and by interpretation of mass spectra. The first compound identified with less retention time (30.236 min) was 2,6-Difluorobenzoic acid, tridec-2-ynyl ester, whereas gamma.-Tocopherol was the last compound which took longest retention time (29.84min) to identify. Many of them are used in industry for various applications like flavor, antioxidant, anti-inflammatory, antimicrobial, pesticide and cancer preventive. Keywords: Freshwater mussels, Parreysia corrugata, GC-MS, Bioactive components

scholarly journals GC-MS analysis of root methanolic extract of Gynochthodes ridsdalei Razafim. and B. Bremer, an endemic, endangered species of southern Western Ghats of India

2018 ◽  
Vol 5 (2) ◽  
pp. 64-67 ◽  
Author(s):  
Renji R Nair ◽  
A Gangaprasad
Keyword(s):  
Retention Time ◽  
Methanolic Extract ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Active Components ◽  
Ms Analysis ◽  
Western Ghats Of India ◽  
Southern Western Ghats ◽  
Kerala State

The present investigation was carried out to determine the presence of biologically active components in the root methanolic extract of Gynochthodes ridsdalei using Gas Chromatography–Mass Spectrometry (GC-MS) analysis. Fresh root of G. ridsdalei collected from the forest areas of Ponmudi hills of Thiruvananthapuram district of Kerala State, India was used for the study. The active principles with their retention time, peak area, molecular weight and molecular formula of the compounds were detected. The analysis revealed the presence of 26 components. The components were identified by comparing their retention time and peak area with that of literature available and by the interpretation of mass spectra.

Determination of Antioxidant Activities and Bioactive Components of Foeniculum vulgare Mill. (Samonsabar)

2019 ◽  
Vol 30 (3) ◽  
pp. 16-22
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Scientific Information ◽  
Ethanolic Extract ◽  
The Body ◽  
World Health ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Bioactive Components ◽  
Foeniculum Vulgare

World Health Organization (WHO) estimated that 80% of the population of developing countries use traditional medicines, mostly natural plant products, for their primary health care needs. In the past few decades, the medicinal value of plants has been assumed more important dimension owing largely to the discovery that extracts from plants contain not only primary metabolites but also a diverse array of secondary metabolites with antioxidant potential. Medicinal plants are potential sources of natural compounds with biological activities and therefore attract the attention of researchers worldwide. Antioxidants are vital substances which possess ability to protect the body from damage due to free radical-induced oxidative stress. The purpose of current study was to determine the antioxidant activities and bioactive components of Foeniculum vulgare (fennel) (Samonsabar) seeds by using UV Visible Spectrophotometer (UV-Vis) and Gas Chromatography-Mass Spectrometry (GC-MS). Aqueous extract of fennel seeds showed more antioxidant activity (IC50: 0.28 ug/ml) than ethanolic extract (IC50: 0.83 ug/ml) and comparable to standard antioxidant, ascorbic acid (IC50: 0.59 ug/ml). GC-MS analysis was fruitful in identification of compounds based on peak area, retention time, molecular formula, molecular weight, MS Fragmentions and pharmacological actions. Ten bioactive phytochemical compounds from aqueous extracts and 11 from ethanolic extract of fennel seeds were identified. These findings indicated that fennel seeds are potential to provide preventive properties against oxidative damage. These results will give scientific information for quality control of indigenous drug to herbal medicine users and local practitioners using fennel for different types of ailments

A Modified QuEChERS Extraction and LC-MS/MS Method for the Determination of Pesticide Residues in Curry Leaves (Murraya koenigii)

2019 ◽  
Vol 6 (1) ◽  
pp. 30-41
Author(s):  
Ranjith Arimboor ◽  
Karunkara Ramakrishna Menon ◽  
Natarajan Ramesh Babu ◽  
Haneesh Chandran
Keyword(s):  
Sample Preparation ◽  
Matrix Effect ◽  
Retention Time ◽  
Pesticide Residues ◽  
Mobile Phase ◽  
Preparation Technique ◽  
Modified Quechers ◽  
Curry Leaves ◽  
Ms Analysis ◽  
The Matrix

Background:Increased consumer demand for curry leaves free from pesticides demands fast and reliable analytical methods for the analysis of pesticide residues.Objective:The optimization of a QuEChERS based sample preparation technique with improved analytical accuracy by removing interfering matrix components for LC-MS/MS analysis of pesticide residues from curry leaves.Methods:A modified QuEChERS solid phase extraction method was developed and validated for the analysis of 26 pesticides in fresh and dried curry leaves. The effects of the sample preparation steps and column retention time on the matrix suppression of analyte ions were also evaluated.Results:Validation parameters were found within an acceptable range. The matrix effect evaluation studies showed that the QuEChERS sample preparation was able to minimize the ion suppression of analytes due to co-eluting matrix of components and that a d-SPE clean up step had major role in reducing matrix effect. The gradient mobile phase with longer retention time for analytes resulted in comparatively lesser matrix effects than the isocratic mobile phase of non-polar nature. Even after the clean up, a considerable number of compounds had more than 20% reduction in their MS response in the gradient mobile phase.Conclusion:This study emphasized the need of proper sample clean up before a LC-MS/MS analysis and the usage of matrix matched standards and mobile phase that ultimately results in an appropriate analyte separation in reasonable retention times.

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