GC-MS Analysis of Antibacterial Phytochemicals from Cassia sophera Linn

2020 ◽  
Vol 13 (5) ◽  
pp. 5131-5137
Author(s):  
Dr. Shyamal K. Jash ◽  
Dilip Gorai ◽  
Lalan Chandra Mandal ◽  
Sekhar Pal
Keyword(s):  
Chemical Constituents ◽  
Research Work ◽  
Enterobacter Aerogenes ◽  
Gas Chromatography Mass Spectrometry ◽  
Gram Negative Bacteria ◽  
Research Groups ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Ms Analysis ◽  
The World

Cassia sophera Linn (Leguminosae) plants are widely distributed worldwide, and find immense applications in traditional systems of medicine in many countries. The plant has been partly explored by various research groups in the world. In our present study, two oils (OL-1 & OL-2) from aerial parts and roots of Cassia sophera have been evaluated by GC/MS using Perkin-Elmer Gas Chromatography–Mass Spectrometry and comparison of spectral data with the existing in National Institute of Standards and Technology (NIST) library. GC/MS analysis of the two oils revealed the presence of a number of chemical constituents. Again, the isolated oils exhibited considerable antibacterial activity against two Gram positive bacteria viz. Bacillus megaterium & Staphylococcus aureus and two Gram negative bacteria viz. Escherichia coli & Enterobacter aerogenes. Therefore, the present study will definitely inspire the researchers engaged in this direction to undertake further research work on this plant for searching new lead molecules

scholarly journals Chemical Composition, Antimicrobial, Cytotoxic and Antiplasmodial Activities of Three Sponges from Buton Islands, Indonesia

2017 ◽  
Vol 22 (3) ◽  
pp. 147
Author(s):  
Masteria Yunovilsa Putra ◽  
Tri Aryono Hadi
Keyword(s):  
Fungal Pathogen ◽  
Brine Shrimp ◽  
Antimicrobial Activities ◽  
Marine Sponges ◽  
Sponge Species ◽  
Gram Negative Bacteria ◽  
Significant Activity ◽  
Gram Positive Bacteria ◽  
Ms Analysis ◽  
Inhibition Zones

GC-MS analysis of the crude extracts of three different species of Indonesian marine sponges has been carried out for identification of bioactive compounds. The GC-MS analysis from Haliclona (Gellius) sp., Lamellodysidea herbacea, and Spheciospongia inconstans revealed the presence of 23, 21, 19 various compounds, respectively and mainly sterols and fatty acids. All the sponge species has been evaluated for antimicrobial activities, cytotoxicity using brine shrimp lethality bioassay and heme polymerization inhibitory activity assay for antiplasmodial activity. In this study, all the sponge species showed antimicrobial activities against at leastone of the test strains. Among them, the extract of sponge Lamellodysidea herbacea displayed activity against two Gram-positive bacteria (S. aureus and B. subtilis) and the Gram-negative bacteria V. cholerae, with inhibition zones of 10.3, 9.2 and 9.5 mm, respectively. The sponge Haliclona (Gellius) sp., showed significant activity against fungal pathogen C. albicans. The sponge Haliclona (Gellius) sp., displayed the ability to inhibit heme polymerization indicating an anti-Plasmodium function and also showed potent cytotoxic activity against the brine shrimp Artemia sp. Keywords: GC-MS analysis, antimicrobial, sponges,bioactive

scholarly journals MIRACLE AYURVEDIC HERB - ASHWAGANDHA (WITHANIA SOMNIFERA DUNAL)

2021 ◽  
Vol 9 (8) ◽  
pp. 1778-1788
Author(s):  
Bargale Sushant Sukumar
Keyword(s):  
Withania Somnifera ◽  
Chemical Constituents ◽  
Immunomodulatory Activity ◽  
Indian Ginseng ◽  
Aerial Parts ◽  
Wide Range ◽  
The World ◽  
Anti Inflammatory ◽  
Ayurvedic Herb ◽  
Present World

Ayurveda is successfully implemented as a primary healthcare system in India, and it is flourishing in the promotion of health around the world. Ayurveda aims to create a society happily, healthy, and peaceful. In general, the simple regimes mentioned in Ayurveda texts are of immense use faced by the present world today regarding health pro- motion. Ashwagandha (Withania Somnifera) is usually referred to as 'Indian winter cherry' or 'Indian Ginseng'. It is a shrub grown in India and North America whose roots have been used by Ayurvedic practitioners for thousands of years. Ashwagandha is one of Ayurveda's most essential herbs, used as Rasayana for its wide range of health benefits for centuries. Chemicals constitute of Ashwagandha (Withania Somnifera)- The chemistry of Ashwagan- dha (Withania Somnifera) has been widely studied and numerous categories of chemical constituents have been described, extracted, and isolated, such as steroidal lactones, alkaloids, flavonoids, tannin, etc. More than 12 alka- loids, 40 withanolides and several sitoindosides have currently been isolated from the aerial parts, roots, and berries of the Withania species and have been recorded. The principal biochemical element of Ashwagandha (WS) root is withanolides, which are steroidal alkaloids and steroidal lactones. The pharmacological activity of Ashwagan- dha- It has been shown to have pharmacological importance as an Adaptogen, Antibiotic, abortifacient, aphrodisiac, Astringent, Anti-inflammatory, deobstructive, diuretic, narcotic, sedative, and tonic through centuries of Ayurvedic medicinal practice using Ashwagandha (Withania Somnifera). Along with these Ashwagandha acts as Anti-stress,Anticarcinogenic activity, Anti-inflammatory activity, Anti-aging activity, Cardioprotective activity, hypothyroid activity and Immunomodulatory activity. Keywords: Ashwagandha, Withania Somnifera, Alkaloids, Withanoids, sitoindosides

scholarly journals Chemical Compositions and Antimicrobial Activity of the Essential Oils of Piper abbreviatum, P. erecticaule and P. lanatum (Piperaceae)

2014 ◽  
Vol 9 (12) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Wan Mohd Nuzul Hakimi Wan Salleh ◽  
Farediah Ahmad ◽  
Khong Heng Yen
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
Antimicrobial Activities ◽  
Dilution Method ◽  
Chemical Compositions ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Weak Activity

The study was designed to examine the chemical composition and antimicrobial activities of essential oils extracted from the aerial parts of three Piper species: Piper abbreviatum, P. erecticaule and P. lanatum, all from Malaysia. GC and GC/MS analysis showed qualitative and quantitative differences between these oils. GC and GC-MS analysis of P. abbreviatum, P. erecticaule and P. lanatum oils resulted in the identification of 33, 35 and 39 components, representing 70.5%, 63.4% and 78.2% of the components, respectively. The major components of P. abbreviatum oil were spathulenol (11.2%), ( E)-nerolidol (8.5%) and β-caryophyllene (7.8%), whereas P. erecticaule oil mainly contained β-caryophyllene (5.7%) and spathulenol (5.1%). Borneol (7.5%), β-caryophyllene (6.6%) and α-amorphene (5.6%) were the most abundant components in P. lanatum oil. Antimicrobial activity was carried out using disc diffusion and broth micro-dilution method against nine microorganisms. All of the essential oils displayed weak activity towards Gram-positive bacteria with MIC values in the range 250–500 μg/mL. P. erecticaule oil showed the best activity on Aspergillus niger (MIC 31.3 μg/mL), followed by P. lanatum oil (MIC 62.5 μg/mL). This study demonstrated that the essential oils have potential as antimicrobial agents and may be useful in the pharmaceutical and cosmetics industries.

scholarly journals STUDY ON THE CHEMICAL CONSTITUENTS AND ANTIBACTERIAL ACTIVITY OF ESSENTIAL OIL OF ACORUS CALAMUS L. RHIZOMES OF RUPENDEHI DISTRICT (NEPAL)

2019 ◽  
Vol 23 (1) ◽  
pp. 57-60
Author(s):  
Susan Joshi ◽  
Sundar Bashyal
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Chemical Constituents ◽  
Acorus Calamus ◽  
Gram Negative Bacteria ◽  
Oil Composition ◽  
Gram Positive Bacteria ◽  
Major Chemical ◽  
Total Oil ◽  
Paper Disc

The essential oil from the air dried powder of wild Acorus calamus L. rhizomes was isolated using Clevenger-type hydrodistillation apparatus and its chemical constituents were analyzed by gas chromatography-mass spectrometric (GC-MS) analysis. A total numbers of fourteen chemical constituents were identified and quantified occupying 100 % of total oil composition. The major chemical constituents are reported to be β-asarone (68.96 %), 4-hydroxy-4-methyl-2-pentanone (8.73 %) shyobunone (8.65 %), p-methoxyphenyl butanone (4.16 %) and the minor chemical constituents are δ-cadinene (0.84 %), isoelemicine (2.68 %), muurolene (1.92 %) and cis-ocimene (1.44 %). The antibacterial activity of the essential oil isolated from the wild Acorus calamus L. rhizomes was carried out using paper disc method against one Gram positive bacteria of Staphylococcus aureus and three Gram negative bacteria of Escherichia coli, Klebsiella pneumoniae and Proteus vulgaris. The isolated essential oil from the dry powder of Acorus calamus L. rhizome showed a good potentiality of the antibacterial effect against tested bacteria.

scholarly journals Resistensi Antibakteri pada Pasien Infeksi Saluran Kemih (ISK) dengan Kateterisasi Urin di Bagian Penyakit Dalam RSUD Arifin Achmad Pekanbaru

2017 ◽  
Vol 5 (2) ◽  
pp. 94
Author(s):  
Taswin Yacob ◽  
Rita Endriani ◽  
M. Yulis Hamidy ◽  
M.Arif Budiman
Keyword(s):  
Urine Culture ◽  
Urethral Catheter ◽  
Antibiotics Resistance ◽  
Gram Negative Bacteria ◽  
Indwelling Catheter ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
The People ◽  
The World ◽  
Catheter Urine

Uninary tract infection (UTI) is a common health problem among the people in the world, including Indonesia.Approximately 80% of UTI in hospital are ascociated with urethral catheter used in hospitalized patients. The increaseof catheter- related UTI is olso followed by increase of antibiotic used. The diffrence in distribution of UTI causingbacteria and antibiotics resistance associated with cathetetization can change according to time and place. This stdyaims to find the pattern of antibiotic resistance in the urine of catheter related UTI patient. The research was done inDepartement of Internal Medicine RSUD arifin Achmad Pekanbaru. 31 samples were collected from hospitalizedpatient who used catheter at last 3 days and taken with indwelling catheter urine. Culture, colony count, colonyidentification and resistance test was performed by Kirby Bauer method. The result was interpretated based onClinical and Laboratory Standard Institude (CLSI). UTI found in 19 samples (61.29%), 9 samples founs no bacteria(29.03%) and Candida albicans on 3 samples (9.68%). UTI caused mostly by Gram-negative bacteria (57.89%),Gram- positive bacteria found in 42.11% of samples. The highest resistance (100%) was found on cefotaxim, cefriaxon,cefalexin and tertracyclin. The lowest resistance (73.68%) found on merofenem and co-trimoxazole.

scholarly journals Optimization of the Antioxidant Potentials of Red Pitaya Peels and Its In Vitro Skin Whitening Properties

2018 ◽  
Vol 8 (9) ◽  
pp. 1516 ◽  
Author(s):  
Ramya Vijayakumar ◽  
Siti Abd Gani ◽  
Uswatun Zaidan ◽  
Mohd Halmi
Keyword(s):  
Vitamin C ◽  
Chemical Constituents ◽  
Beta Carotene ◽  
Dry Weight ◽  
Pharmaceutical Formulations ◽  
Gas Chromatography Mass Spectrometry ◽  
Antioxidant Power ◽  
Skin Whitening ◽  
Ms Analysis

In this study, response surface methodology (RSM) was employed for the optimization of the antioxidant potentials of red pitaya peels using independent variables: temperature (45–65 °C), ethanol concentration (70–90%, v/v) and time (80–120 min) through its responses, which were DPPH scavenging activity, ferric ion reducing antioxidant power (FRAP), and beta-carotene bleaching (BCB) inhibition, respectively. In Vitro anti-tyrosinase and vitamin C content assays were carried out spectrophotometrically to determine the skin whitening efficacy of the optimized red pitaya peel extract. A gas chromatography-mass spectrometry (GC-MS) analysis was employed to identify the chemical constituents present in the optimized extract. The optimized conditions were ethanol 82% (v/v) for 103 min at 56 °C with values of 75.98% for DPPH, 7.45 mM Fe2+/g dry weight for FRAP and 93.29% for BCB respectively. The in vitro anti-tyrosinase and vitamin C content evaluation of the optimized extract showed a good tyrosinase inhibition of 66.29% with IC50 of 24.06 µg/mL while the vitamin C content was 5.45 mg/g of the extract. The GC-MS analysis revealed the presence of thirty phytoconstituents with l-(+)-ascorbic acid 2,6-dihexadecanoate being the most abundant with a peak area of 14.66%. This study evidently suggests the potential of red pitaya peels to be exploited as a natural skin whitening agent in the cosmeceutical and pharmaceutical formulations.

scholarly journals In Vitro Pharmacological Activities and GC-MS Analysis of Different Solvent Extracts ofLantana camaraLeaves Collected from Tropical Region of Malaysia

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Mallappa Kumara Swamy ◽  
Uma Rani Sinniah ◽  
Mohd. Sayeed Akhtar
Keyword(s):  
Ethyl Acetate ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Acid Methyl Ester ◽  
Antibacterial Activities ◽  
Tropical Region ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Ms Analysis

We investigated the effect of different solvents (ethyl acetate, methanol, acetone, and chloroform) on the extraction of phytoconstituents fromLantana camaraleaves and their antioxidant and antibacterial activities. Further, GC-MS analysis was carried out to identify the bioactive chemical constituents occurring in the active extract. The results revealed the presence of various phytocompounds in the extracts. The methanol solvent recovered higher extractable compounds (14.4% of yield) and contained the highest phenolic (92.8 mg GAE/g) and flavonoid (26.5 mg RE/g) content. DPPH radical scavenging assay showed the IC50value of 165, 200, 245, and 440 μg/mL for methanol, ethyl acetate, acetone, and chloroform extracts, respectively. The hydroxyl scavenging activity test showed the IC50value of 110, 240, 300, and 510 μg/mL for methanol, ethyl acetate, acetone, and chloroform extracts, respectively. Gram negative bacterial pathogens (E. coliandK. pneumoniae) were more susceptible to all extracts compared to Gram positive bacteria (M. luteus,B. subtilis, andS. aureus). Methanol extract had the highest inhibition activity against all the tested microbes. Moreover, methanolic extract ofL. camaracontained 32 bioactive components as revealed by GC-MS study. The identified major compounds included hexadecanoic acid (5.197%), phytol (4.528%), caryophyllene oxide (4.605%), and 9,12,15-octadecatrienoic acid, methyl ester, (Z,Z,Z)- (3.751%).

scholarly journals Identification of Chemical Compounds from the Ethanolic Extract of the Bark of Bauhinia tomentosa L. By GC-MS Analysis

2016 ◽  
Vol 8 (03) ◽  
Author(s):  
Gopalakrishnan S. ◽  
Vadivel E.
Keyword(s):  
Mass Spectra ◽  
Skin Diseases ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Microbial Infections ◽  
Phytochemical Constituents ◽  
Major Chemical

The bark of Bauhinia tomentosa L. is used wildly used for the treatment of varies ailments such as inflammation, wound, dysentery, skin diseases and for microbial infections. In the present study the ethanolic extract of the bark of Bauhinia tomentosa L. has been subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis revealed the presence of fourteen phytochemical constituents, These compounds were identified by comparing their retention time and peak area with that of literature and by interpretation of mass spectra. The major chemical constituents are 4-(Benzyloxy)-1-methoxy-2(3,7-dimethylocta-2,6-dienyl)-benzene (10.88%), Levodopa (6.39%) and (9E, 12E) - Methyl octadeca-9,12-dienoate (5.68%) which possess many biological activities. Hence these studies pave a platform to screen many bioactive components for wound healing.

scholarly journals Gas chromatography-Mass spectrometry (GC-MS) analysis of bioactive components present in grape citrus peel in Nigeria

2021 ◽  
Vol 8 (1) ◽  
pp. 166-174
Author(s):  
Israel Chukwuemeka Eneke ◽  
Eka Bassey Essien ◽  
Matthew Owhonda Wegu
Keyword(s):  
Retention Time ◽  
Research Work ◽  
Value Added ◽  
Gas Chromatography Mass Spectrometry ◽  
Citrus Paradisi ◽  
Bioactive Components ◽  
Citrus Peel ◽  
Mass Selective Detector ◽  
Ms Analysis ◽  
Grape Peel

Grapefruit (Citrus paradisi) is an important cultivar of the Citrus genus which contains a number of nutrients beneficial to human health. Grapefruit peels are usually thrown away in this part of Nigeria. The research work investigated the bioactive components present in an indigenous citrus peel, grape (Citrus paradisi). Grape fruits were purchased from the fruits garden market in D-Line, Port-Harcourt metropolis and washed with ionized water and allowed to shade dry. The peel of the fruits were separated and subjected to cold extraction using 95% ethanol. The extracts obtained were further extracted in dichloromethane and subjected to GC/MS analysis for characterization of various bioactive components. The gas chromatographic model: 789A (GC) analysis was performed on an agilent technologies interfaced with mass selective detector model: 5975(MSD). The results revealed 25 bioactive components in grape peel with n-Hexadecanoic acid showing the highest concentration of 20.36% and retention time of 18.522min. Nootkatone was the lowest component in the grape peel with concentration 0.74% and retention time of 16.459min. Results shows that grapefruit (Citrus paradisi) has considerable potential as a source of natural bioactive components with different retention times. These fruits residues which otherwise regarded as waste hold promising potentials for medicinal therapy and value added food supplements.

scholarly journals GC-MS ANALYSIS OF BIOACTIVE CONSTITUENTS OF JASMINE FLOWER

2018 ◽  
Vol 4 (1) ◽  
pp. 52-59
Author(s):  
Hesham Hussein Rassem ◽  
Abdurahman Hamid Nour ◽  
Rosli Mohammed Yunus
Keyword(s):  
Phthalic Acid ◽  
Mass Spectra ◽  
Chemical Constituents ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Constituents ◽  
Microwave Assisted ◽  
Flower Extract ◽  
Ms Analysis ◽  
Traditional Practitioners ◽  
Comparison Of Mass Spectra

Gas Chromatography-Mass Spectrometry (GC-MS) the best technique to identified the compounds of essential oils by comparison of mass spectra data obtained from the sample with that taken from pure commercially available standards injected under the same conditions. To characterize the chemical constituents of Hibiscus Flower using GC-MS, the shade dried flower powder was extracted with methanol by using Microwave-assisted Hydrodistillation (MAHD). The GC-MS analysis provided different peaks determining the presence of ten compounds. These compounds havebiological activity namely 2-Phenylthiolane (57.31%), Cyclohexene, 3-ethenyl- (25.91%), Acetaldehyde (12.70%), N-Methylallylamine (9.99%),  ropanamide (6.79%) and Phthalic acid, bis (7-methyloctyl) ester (5.21%). From the results, it can be concluded that Jasmine flower extract shows the presence of 10 phytocompounds. The presence of various bioactive compounds justifies the use of the jasmine flower for various ailments by traditional practitioners.

Sign in / Sign up

Export Citation Format

Share Document