scholarly journals Phytochemical analysis and antibacterial activity of Psidium guajava L. leaf extracts

2017 ◽  
Vol 1 (2) ◽  
pp. 013-019 ◽  
Author(s):  
Kenneth Ekeleme ◽  
Paul Tsaku ◽  
Istifanus Nkene ◽  
Uba Ufomadu ◽  
Rejoice Abimiku ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Psidium Guajava ◽  
Phytochemical Analysis ◽  
Leaf Extracts

scholarly journals The Efficacy of Psidium guajava Linn Leaf Extractsfrom Selangor Region Against Gram-Positive and Gram-Negative Bacteria

2018 ◽  
Vol 54 (4) ◽  
pp. 294
Author(s):  
S Zuhaira ◽  
Noorhaniz Mohd Nizam ◽  
PM Ridzuan
Keyword(s):  
Methanol Extract ◽  
Psidium Guajava ◽  
Diffusion Method ◽  
Future Research ◽  
Phytochemical Analysis ◽  
Gram Negative Bacteria ◽  
Leaf Extracts ◽  
Gram Positive ◽  
Gram Negative ◽  
Freeze Dried

Antibiotic is a type of medication that helps in fighting bacterial infection. Treating bacterial infections in clinical setting become more complicated and costly due to drug resistance. This study was conducted to determine the antibacterial potential of Psidium guajava Linn leaf extracts against Gram-positive and Gram-negative bacteria. P. guajava Linn leaf was obtained from Research Orchards at University Putra Malaysia (UPM). Leaves were extracted using three types of extracts; hot, cold and methanol extract. Freeze dried was used in this study and temperature was set at -104°C for 24 hours. Antibacterial testing was determined using disc diffusion and well diffusion method. Antibacterial activity was evaluated by measuring the inhibitory zone of the tested bacteria. Phytochemical analysis was conducted by adding few drop of diluted NaOH (Sodium Hydroxide) solution to detect the active flavanoid from leaves extract. Antimicrobial activity showed all extracts were effective against Gram-positive and Gram-negative bacteria. Phytochemical analysis of P. guajava Linn leaf extracts showed the methanol extract indicating the presence of tannins, phenols, flavonoids, terpenoids, glycosides and saponins. Results showed that P. guajava Linn leaf was effective against Gram-positive and Gram-negative bacteria, and pythochemical analysis also exhibited a few active compounds that were determined. P. guajava Linn leaf had potential natural product that may be used for further analysis in the future research.

scholarly journals ANTI BACTERIAL AND BIOFILM INHIBITORY ACTIVITIES OF AEGLE MARMELOS METHANOL LEAF EXTRACT

2021 ◽  
Vol 9 (09) ◽  
pp. 165-173
Author(s):  
Gujjari Sreehitha Pratap ◽  
Keyword(s):  
Antibacterial Activity ◽  
Biologically Active ◽  
Diffusion Method ◽  
Antibiofilm Activity ◽  
Phytochemical Analysis ◽  
Aegle Marmelos ◽  
Leaf Extracts ◽  
Biofilm Inhibition ◽  
Microbial Strains ◽  
Elisa Plate

The Aegle marmelos commonly known as BAEL belongs to family Rutaceae plays a role in traditional culture and medication from ancient periods. This plant lacks sufficient evidences regarding the values and components it has. Therefore, we framed out our studies to evaluate the phytochemical analysis, antibacterial activity, antibiofilm activity. These studies are evaluated using different solvents like methanol, acetone, chloroform, toluene leaf extracts of Aegle marmelos. We evaluated the potency of different solvents leaf extracts using Agar well diffusion method. Antibacterial activity was also evaluated using ELISA plate assay. The potency of different solvents extracts to inhibit biofilm of selected microbial strains. In accordance to results, the leaf extracts revealed the presence of several biologically active phytochemicals with highest quantities of carbohydrates, phenols, alkaloids, flavonoids, tannins, saponins, steroids, aminoacids etc. The antibacterial activity was found significant against microbial strains of both gram positive and gram negative bacteria. These strains showed susceptibility nature towards the different solvents extracts with zone of inhibitions (mm). On the other hand, the inhibition of biofilm was also significant at all tested concentrations. The biofilm inhibition of microbial strains was found significant at 1 XMIC, 2 XMIC, 3 XMIC. Based on our studies here we conclude that the different solvents leaf extracts possessed inhibitory activity against selected human pathogenic organisms.

scholarly journals Phytochemical analysis and antibacterial activity of extract from lantana camara l. leaves

2021 ◽  
Author(s):  
Bondy Lourenço ◽  
Asimbawe Kiza ◽  
Abrão Amândio João ◽  
Clemência Félix Odala Niconte ◽  
Pompílio Armando Vintuar ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Lantana Camara ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Significant Difference ◽  
New Antibiotics

Abstract Background: Currently, several studies are being conducted to test antibacterial activity of various medicinal plants, in attempt to develop new antibiotics or to potentiate the action of antibiotics of known clinical use. In this research, phytochemical analysis and in vitro antibacterial activity of leaf extracts of Lantana camara L were performed.Methods The extracts were obtained through maceration with 90% ethanol. In phytochemical analysis, alkaloids, flavonoids, tannins and saponins were identified using specific reagents for each class.The antibacterial activity was analyzed using the disk diffusion method according to Kirby-Bauer, against Escherichia coli and Staphylococus aureus.Key findings The diameters of the inhibition halos varied between 0.7 and 12 mm, with an average of 8.26 mm for strains of S. aureus and there was no inhibition for E. coli. The results showed a significant difference between the inhibition halos and the tested concentrations for S. aureus. In conclusion L. camara leaf extracts are rich in bioactive compounds and have a good antibacterial potential against S. aureus and not for E. coli. And there was no relationship between the concentration of the extract and the diameter of the halo of inhibition of the evaluated microorganism.

scholarly journals Cytotoxic activity of silver nanoparticles prepared from Psidium guajava L. (Myrtaceae) and Lawsonia inermis L. (Lythraceae) extracts

2021 ◽  
Vol 20 (9) ◽  
pp. 1791-1799
Author(s):  
Fatma M. Abdel Bar ◽  
Mohamed M Abu Habib ◽  
Fardous F. El-Senduny ◽  
Farid A. Badria
Keyword(s):  
Silver Nanoparticles ◽  
Cytotoxic Activity ◽  
Antioxidant Properties ◽  
Psidium Guajava ◽  
Cytotoxic Activities ◽  
Phytochemical Analysis ◽  
Lawsonia Inermis ◽  
Leaf Extracts ◽  
Cancer Treatments ◽  
Transmission Electron

Purpose: To biosynthesize silver nanoparticles (AgNPs) using Psidium guajava L. and Lawsonia inermis L. leaf extracts, and investigate their antioxidant and cytotoxic activities.Methods: The aqueous extracts were prepared by maceration in distilled H2O followed by partitioning with EtOAc. AgNPs were prepared by treating the extracts with 1 mM AgNO3 and then were characterized by UV-vis and FTIR analyses, and transmission electron microscopy (TEM). MTT cytotoxicity and 2,2`-azinobis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) antioxidant assays were used to assess their cytotoxic and antioxidant properties, respectively.Results: AgNPs from P. guajava and L. inermis extracts exhibited good morphological stability and showed moderate antioxidant activity (68.1 and 71.9%, respectively) compared to their extracts. Equipotent cytotoxicity against HCT-116 and MCF-7 cells was observed for AgNPs derived from P.guajava, while AgNPs derived from L. inermis possessed two-fold cytotoxicity compared to their corresponding extracts. Phytochemical analysis of P. guajava afforded pyrogallol, quercetin, quercetin-3-O-β-xylopyranoside, quercetin-3-O-β-arabinopyranoside, and quercetin-3-O-α-rabinofuranoside, while L. inermis afforded lawsone and luteolin.Conclusion: Flavonoids and phenolics play a major role in reducing Ag+ ions, surface coating, antioxidant, and cytotoxic activities of AgNPs. The biocompatible AgNPs produced by L. inermis demonstrate promising cytotoxic activity that could contribute to new cancer treatments.

scholarly journals Evaluation of the Phytochemical and Antibacterial characteristics of leaf extracts of Xanthium strumarium L. against Bacteria

2020 ◽  
Vol 11 (1) ◽  
pp. 725-729
Author(s):  
Antu Kurrey ◽  
Lata Sharma ◽  
Shishir Tiwari
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Herbal Medicines ◽  
Wild Plant ◽  
Xanthium Strumarium ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Leaf Extracts ◽  
Maximum Inhibition

Plants are one of the large sources of herbal medicines. Many plants have the capability to produce some bioactive constituents which give defense against microorganisms like bacteria and fungi and also from insects. Xanthium strumarium L. is a wild plant that grows like a weed. Leaves of  Xanthium strumarium L. were subjected for extraction in 4 different solvents viz. methanol, chloroform, aqueous, and ethanol. The aim of this study was to investigate the phytochemical screening and antimicrobial activity of methanol, chloroform, aqueous, and ethanol extracts prepared from leaves of  Xanthium strumarium L. The antibacterial activity was assessed by using the agar well diffusion assay against tested bacterial strain, Escherichia coli (CGSC 4312) and Bacillus subtilis (ATCC 9634). Phytochemical analysis has revealed that  Xanthium strumarium L. has most of the significant phytoconstituents like Saponins, Terpenoids, Flavanoids, Phenol, steroids, Anthraquinones, Tannins,  Alkaloids,  Glycosides, and Carbohydrate. The zone of inhibition was measured and compared by standard antibiotic streptomycin. The outcome of the present work showed that chloroform extract (400µg/ml) showed maximum inhibition against E. Coli (23mm), and Ethanol extract(400µg/ml) showed maximum inhibition against Bacillus subtilis (24mm). The result divulged the presence of antibacterial activity of different extracts of Xanthium strumarium L.against human pathogenic bacteria. Thus, this plant has many active compounds that can be used for the development of various potent drugs.

scholarly journals Phytochemical Analysis and Antibacterial Activity of Extract From Lantana Camara L. Leaves

2021 ◽  
Author(s):  
Bondy Jorge Lourenço ◽  
Asimbawe Kiza ◽  
ABRÃO AMÂNDIO JOÃO ◽  
Clemência FÉLIX ODALA Niconte ◽  
POMPÍLIO ARMANDO Vintuar ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Lantana Camara ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Significant Difference ◽  
New Antibiotics

Abstract Background: Currently, several studies are being conducted to test antibacterial activity of various medicinal plants, in attempt to develop new antibiotics or to potentiate the action of antibiotics of known clinical use. In this research, phytochemical analysis and in vitro antibacterial activity of leaf extracts of Lantana camara L were performed.The extracts were obtained through maceration with 90% ethanol. In phytochemical analysis, alkaloids, flavonoids, tannins and saponins were identified using specific reagents for each class.The antibacterial activity was analyzed using the disk diffusion method according to Kirby-Bauer, against Escherichia coli and Staphylococus aureus. Results: The diameters of the inhibition halos varied between 0.7 and 12 mm, with an average of 8.26 mm for strains of S. aureus and there was no inhibition for E. coli. The results showed a significant difference between the inhibition halos and the tested concentrations for S. aureus. Conclusions: L. camara leaf extracts are rich in bioactive compounds and have a good antibacterial potential against S. aureus and not for E. coli. And there was no relationship between the concentration of the extract and the diameter of the halo of inhibition of the evaluated microorganism.

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