ANTI BACTERIAL AND BIOFILM INHIBITORY ACTIVITIES OF AEGLE MARMELOS METHANOL LEAF EXTRACT

The Aegle marmelos commonly known as BAEL belongs to family Rutaceae plays a role in traditional culture and medication from ancient periods. This plant lacks sufficient evidences regarding the values and components it has. Therefore, we framed out our studies to evaluate the phytochemical analysis, antibacterial activity, antibiofilm activity. These studies are evaluated using different solvents like methanol, acetone, chloroform, toluene leaf extracts of Aegle marmelos. We evaluated the potency of different solvents leaf extracts using Agar well diffusion method. Antibacterial activity was also evaluated using ELISA plate assay. The potency of different solvents extracts to inhibit biofilm of selected microbial strains. In accordance to results, the leaf extracts revealed the presence of several biologically active phytochemicals with highest quantities of carbohydrates, phenols, alkaloids, flavonoids, tannins, saponins, steroids, aminoacids etc. The antibacterial activity was found significant against microbial strains of both gram positive and gram negative bacteria. These strains showed susceptibility nature towards the different solvents extracts with zone of inhibitions (mm). On the other hand, the inhibition of biofilm was also significant at all tested concentrations. The biofilm inhibition of microbial strains was found significant at 1 XMIC, 2 XMIC, 3 XMIC. Based on our studies here we conclude that the different solvents leaf extracts possessed inhibitory activity against selected human pathogenic organisms.

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ANTIBACTERIAL AND ANTIBIOFILM ACTIVITY OF QUERCUS INFECTORIA GALLS ON ROTHIA DENTOCARIOSA ISOLATED FROM DENTAL CARIES

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i10.34725 ◽

2019 ◽

pp. 159-162

Author(s):

AMBULKAR S ◽

TALE V ◽

KHILARI S ◽

PAWAR J

Keyword(s):

Dental Caries ◽

16S Rrna ◽

Microtiter Plate ◽

Diffusion Method ◽

Antibiofilm Activity ◽

Phytochemical Analysis ◽

16S Rrna Sequence ◽

Biofilm Inhibition ◽

Microtiter Plate Assay ◽

Quercus Infectoria

Objective: The present study aimed to study Quercus infectoria gall extract for phytochemical analysis, antibacterial, and antibiofilm activity against Rothia dentocariosa isolated from dental caries. Methods: R. dentocariosa was isolated, characterized, and identified by 16S rRNA sequence and also checked for biofilm formation ability. Phytochemical analysis of Q. infectoria aqueous gall extracts was carried out. Antibacterial and antibiofilm activity was performed using agar well diffusion method and microtiter plate assay, respectively. Results: Bacterial isolate from dental caries was identified as R. dentocariosa by 16s rRNA sequencing technique with accession number MH824681 obtained from NCBI. Phytochemical analysis of Q. infectoria aqueous gall extract revealed the presence of alkaloids, phenol, tannin, glycosides, phenolic compound, and flavonoids. Significant antibacterial activity was observed with 19.00 (±7.07) mm diameter zone of inhibition. The biofilm inhibition assay was performed by microtiter plate method indicated 92.89% inhibition of bacteria at the concentration of 100 mg/mL of aqueous extract. Conclusion: The results indicated the efficacy of Q. infectoria gall extracts that could be explored as an alternative to current treatment.

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Phytochemical analysis and antibacterial activity of extract from lantana camara l. leaves

10.21203/rs.3.rs-151542/v1 ◽

2021 ◽

Author(s):

Bondy Lourenço ◽

Asimbawe Kiza ◽

Abrão Amândio João ◽

Clemência Félix Odala Niconte ◽

Pompílio Armando Vintuar ◽

...

Keyword(s):

Antibacterial Activity ◽

Lantana Camara ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Disk Diffusion Method ◽

E Coli ◽

Significant Difference ◽

New Antibiotics

Abstract Background: Currently, several studies are being conducted to test antibacterial activity of various medicinal plants, in attempt to develop new antibiotics or to potentiate the action of antibiotics of known clinical use. In this research, phytochemical analysis and in vitro antibacterial activity of leaf extracts of Lantana camara L were performed.Methods The extracts were obtained through maceration with 90% ethanol. In phytochemical analysis, alkaloids, flavonoids, tannins and saponins were identified using specific reagents for each class.The antibacterial activity was analyzed using the disk diffusion method according to Kirby-Bauer, against Escherichia coli and Staphylococus aureus.Key findings The diameters of the inhibition halos varied between 0.7 and 12 mm, with an average of 8.26 mm for strains of S. aureus and there was no inhibition for E. coli. The results showed a significant difference between the inhibition halos and the tested concentrations for S. aureus. In conclusion L. camara leaf extracts are rich in bioactive compounds and have a good antibacterial potential against S. aureus and not for E. coli. And there was no relationship between the concentration of the extract and the diameter of the halo of inhibition of the evaluated microorganism.

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Phytochemical Analysis and Antibacterial Activity of Extract From Lantana Camara L. Leaves

10.21203/rs.3.rs-156702/v1 ◽

2021 ◽

Author(s):

Bondy Jorge Lourenço ◽

Asimbawe Kiza ◽

ABRÃO AMÂNDIO JOÃO ◽

Clemência FÉLIX ODALA Niconte ◽

POMPÍLIO ARMANDO Vintuar ◽

...

Keyword(s):

Antibacterial Activity ◽

Lantana Camara ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Disk Diffusion Method ◽

E Coli ◽

Significant Difference ◽

New Antibiotics

Abstract Background: Currently, several studies are being conducted to test antibacterial activity of various medicinal plants, in attempt to develop new antibiotics or to potentiate the action of antibiotics of known clinical use. In this research, phytochemical analysis and in vitro antibacterial activity of leaf extracts of Lantana camara L were performed.The extracts were obtained through maceration with 90% ethanol. In phytochemical analysis, alkaloids, flavonoids, tannins and saponins were identified using specific reagents for each class.The antibacterial activity was analyzed using the disk diffusion method according to Kirby-Bauer, against Escherichia coli and Staphylococus aureus. Results: The diameters of the inhibition halos varied between 0.7 and 12 mm, with an average of 8.26 mm for strains of S. aureus and there was no inhibition for E. coli. The results showed a significant difference between the inhibition halos and the tested concentrations for S. aureus. Conclusions: L. camara leaf extracts are rich in bioactive compounds and have a good antibacterial potential against S. aureus and not for E. coli. And there was no relationship between the concentration of the extract and the diameter of the halo of inhibition of the evaluated microorganism.

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Investigation of the Phyto-constituents and the Antibacterial Activity of Burkea africana against P. aeruginosa, E. coli, S. aureus and E. faecalis

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2021/v30i430262 ◽

2021 ◽

pp. 33-40

Author(s):

S. Zengeni ◽

J. Chifamba ◽

I. Mutingwende ◽

T. Manyarara

Keyword(s):

Antibacterial Activity ◽

Water Extract ◽

Ethanolic Extract ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Antibacterial Efficacy ◽

Bacterial Strains ◽

Zone Of Inhibition ◽

Leaf Extracts ◽

E Coli

Bacterial skin infections are common in the tropics and the emergence of resistant bacterial strains has complicated treatment outcomes especially in immune compromised patients. This study focused on determining the antibacterial activity and preliminary phytochemical screening of the plant, Burkea Africana, which has been used for decades to treat various ailments in Southern Africa. Phyto-constituent determination and antibacterial assay was carried out on the hydro-ethanolic and aqueous leaf extracts of Burkea Africana. The Agar well diffusion method was used to investigate the antibacterial efficacy of both extracts. Activity was measured against P. aeruginosa, E. coli, S. aureus and E. faecalis using ciprofloxacin as the standard. For both extracts, phytochemical analysis revealed the presence of steroids, tannins, saponins and carbohydrates. The 70% hydro-ethanolic extract however revealed more phyto-constituents including flavonoids and alkaloids. For both extracts antibacterial activity was comparable to the standard. The highest zone of inhibition recorded for the water extract was 22±0.4 mm at 500 mg/ml for E. coli while the standard, Ciprofloxacin had a zone of inhibition of 25±0.33 mm. The ethanolic extract showed higher antibacterial efficacy when compared to ciprofloxacin against S. aureus. The zone of inhibition for the 70% ethanolic extract was 25.4±0.50 mm and that of the standard was 27±0.48 mm. Antibacterial activity was generally higher in the ethanolic extract than the water extract. It is therefore concluded that B. africana exhibits antibacterial activity.

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Phytochemical analysis and antibacterial activity of extract from lantana camara l. leaves

10.21203/rs.3.rs-151542/v2 ◽

2021 ◽

Author(s):

Bondy Lourenço ◽

Asimbawe Kiza ◽

Abrão Amândio João ◽

Clemência Félix Odala Niconte ◽

Pompílio Armando Vintuar ◽

...

Keyword(s):

Antibacterial Activity ◽

Lantana Camara ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Disk Diffusion Method ◽

E Coli ◽

Significant Difference ◽

New Antibiotics

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PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF IPOMOEA INDICA PLANT LEAF EXTRACTS

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2018v10i2.25879 ◽

2018 ◽

Vol 10 (2) ◽

pp. 86

Author(s):

Seenu Yuvarani ◽

Selvam .

Keyword(s):

Antibacterial Activity ◽

Aqueous Extract ◽

Salmonella Typhi ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Plant Leaves ◽

Bacterial Strains ◽

Zone Of Inhibition ◽

Leaf Extracts ◽

Plant Leaf

Objective: The objective of this study was to phytochemical analysis and antibacterial activity of the Ipomoea indica plant leaves extracts.Methods: The crude powder of the plant leaf samples of I. indica was extracted using methanol and aqueous solvents and subjected to qualitative phytochemical analysis. The antibacterial activity of the aqueous and methanol extracts was tested at different for concentrations. The extract was tested against bacterial strains like Staphylococcus aureus, Enterococcus faecalis, Salmonella typhi, and Escherichia coli.Results: The phytochemical screening revealed the presence of alkaloids, flavonoids, terpenoids, glycoside, reducing sugars, saponins, steroids and tannins in the solvent extract. The antibacterial activity was performed by using the disc diffusion method, where the largest zone of inhibition was obtained with aqueous extract 75µ concentration against Salmonella typhi (26 mm). The antibacterial activity was compared with the standard antibiotics like Streptomycin.Conclusion: This study suggests that the aqueous extract of the I. indica leaves possesses bioactive compounds with antibacterial activity against the bacterial strains, which can be used to formulate new antibacterial drugs for disease control.

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EVALUATION OF ANTIBACTERIAL ACTIVITY OF INDONESIAN VARIETIES SWEET POTATO LEAVES EXTRACT FROM CILEMBU AGAINST Shigella dysenteriae ATCC 13313

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i2.15773 ◽

2017 ◽

Vol 10 (2) ◽

pp. 377 ◽

Cited By ~ 1

Author(s):

Sri Agung Fitri Kusuma

Keyword(s):

Antibacterial Activity ◽

Sweet Potato ◽

Ipomoea Batatas ◽

Ethanol Extract ◽

Biologically Active ◽

Shigella Dysenteriae ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Dependent Manner ◽

Sweet Potato Leaves

Objective: The aim of this study was to evaluate the in Vitro antibacterial activity of sweet potato (Ipomoea batatas (L.) Lam) leaves ethanol extract against Shigella dysenteriae ATCC 13313.Methods: The study was performed by collecting and determining plant samples, extraction, moisture content assay, phytochemical screening, Thin layer Chromatography, antibacterial activity using agar diffusion method using perforator of 11 mm in diameter and Minimum Inhibition Concentration (MIC) test using macrodilution method against Shigella dysenteriae ATCC 13313.Results: The Phytochemical analysis of the sweet potato leaves extract revealed the presence of flavonoids, tannin, steroid and polyphenolic compound. The sweet potato leaves ethanol extract showed significant zone of inhibition in a dose dependent manner against Shigella dysenteriae ATCC 13313 with the range of MIC and MBC was 10-20 % b/v.Conclusion : It can be concluded that the sweet potato leaves ethanol extract shows promise as an antibacterial agent for inhibiting bacillary dysentery due to the presence of biologically active ingredients with antimicrobial activity in the extract.Keywords: sweet potato, Cilembu, Indonesia, antidysentery, Shigella dysenteriae ATCC 13313

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PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTIMICROBIAL, AND ANTIBIOFILM ACTIVITY OF SAUROPUS ANDROGYNUS LEAF EXTRACTS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i4.31756 ◽

2019 ◽

pp. 244-250 ◽

Cited By ~ 1

Author(s):

SUJITHA KUTTINATH ◽

HARITHA KH ◽

RAM RAMMOHAN

Keyword(s):

Plant Extracts ◽

Methanolic Extract ◽

Extraction Procedure ◽

Radical Scavenging ◽

Microtiter Plate ◽

Biologically Active ◽

Antibiofilm Activity ◽

Phytochemical Analysis ◽

Phytochemical Screening ◽

Leaf Extracts

Objective: The objective of the study was to perform phytochemical screening, and evaluate the antioxidant, antimicrobial, and antibiofilm activities of Sauropus androgynus leaf extract against pathogenic organisms. Methods: Sauropus androgynus leaves were shade dried, powdered, and extracted by Soxhlet hot extraction procedure using ethanol or methanol. The aqueous extract was prepared using a rotary shaker. The qualitative phytochemical screening was carried out by gas chromatography-mass spectroscopic (GC-MS) analysis and liquid chromatography-mass spectrometry (LC-MS). The antioxidant activity of plant extracts was estimated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH)-free radical scavenging assay. Antimicrobial and antibiofilm activity of extracts against various pathogens was performed using serial microdilution assay and microtiter plate method, respectively. Results: Preliminary phytochemical analysis showed the presence of compounds such as tannins, flavonoids, alkaloids, and phenols. GC-MS studies also revealed the presence of biologically active compounds. Methanolic extract of S. androgynus showed higher DPPH Scavenging activity compared to other extracts. All the extracts were able to inhibit both bacterial growth and biofilm production; however, the methanolic extract showed the highest antibiofilm activity. Conclusion: S. androgynus plant extracts possess significant antioxidant, antimicrobial, and antibiofilm activity.

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In Vitro Antibacterial Activity of Gynandropsis gynandra on some Pathogenic Enteric Bacterial Isolates

Archives of Health Science ◽

10.31829/2641-7456/ahs2021-5(1)-010 ◽

2021 ◽

pp. 1-7

Keyword(s):

Antibacterial Activity ◽

Salmonella Typhi ◽

Antimicrobial Activities ◽

Biologically Active ◽

Diffusion Method ◽

Bioactive Constituents ◽

Leaf Extracts ◽

Zones Of Inhibition ◽

Gynandropsis Gynandra

Plants contain many biologically active compounds that have potential for development as medicinal agents. The study was conducted to investigate the antimicrobial activities of Gynandropsis gynandra (leaves and stem) and its major bioactive constituents (phytochemicals). The aqueous and ethanol extracts from the leaves and stem of the plant was tested using well Diffusion method for their antimicrobial activity against some members Enterobacteriales family (Escherichia coli, Shigella sp, Klebsiella sp and Salmonella typhi) isolated from diarrheic stool sample from Murtala Muhammad specialist Hospital, Kano. Preliminary phytochemical analyses showed that both stem and leaf extracts contain alkaloids, tannins, terpenoid, Anthraquinones, reducing sugar, amino acid, flavonoids, saponins, cardiac glycosides, resin and phenols. The result shows that the extracts demonstrated higher antibacterial activity against the isolates tested with the average zone of inhibition of 16.60 mm, 16.54 mm, 14.73 mm and 13.95 mm for Shigella sp, E. coli, Klebsiella sp and S. typhi respectively. The result also demonstrated that leaf extract is more effective than the stem extract. The ethanolic extracts of the plant showed higher zones of inhibition against test organisms (17.74 mm) compared to aqueous extracts (13.15 mm). There is no significant different on the susceptibility of the organisms tested against the extracts at p

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The In vitro Evaluation of the Antimicrobial Activity of Quercus robur L. Methanolic and Aqueous Leaves’ Extracts, from the Algerian High Plateaus Against some Uropathogenic Microbial Strains

Phytothérapie ◽

10.3166/phyto-2019-0133 ◽

2019 ◽

Vol 18 (5) ◽

pp. 262-274

Author(s):

E. Benyagoub ◽

N. Nabbou ◽

S. Boukhalkhel ◽

I. Dehini

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Enterococcus Faecalis ◽

Fusidic Acid ◽

Quercus Robur ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Microbial Strains

The medicinal value of the plants is due to their chemical components that bring a definite physiological action on the human body to prevent the diseases. In this work, we investigated the antimicrobial activity of leaves’ extracts of Quercus robur L., collected from the Algerian upper highlands, on ten bacterial strains and one fungal strain known to be pathogenic. First, we performed a qualitative phytochemical analysis, and second, antimicrobial activity tests performed by agar diffusion method (disc and well) with the determination of MIC by broth macro-dilution method. Given the results, it appears that obtained macerates of Quercus robur L. were rich in bioactive phytoconstituents such as alkaloids, anthraquinones, saponins, tannins, and other components. The yield of aqueous and methanolic macerates of leaves was 8.5 ± 1.41 and 22.4 ± 4.36%, respectively. The bacterial resistance was relatively important to several antibiotics, namely, ampicillin, amoxicillin + clavulanic acid for strains of Escherichia coli and Salmonella sp. However, Staphylococcus aureus strains were resistant to fusidic acid, penicillin, and oxacillin; while Enterococcus faecalis was resistant to fusidic acid, penicillin, oxacillin, and ticarcillin. The antibacterial activity of the macerates toward tested microbial strains showed that the aqueous and methanolic macerates of the leaves were proportional to the tested concentration and active not only against Gram-positive and Gram-negative bacteria but also on the fungal species Candida albicans. The estimated MIC for Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus was in the order of 10 mg/mL, which seems more effective than toward Salmonella sp., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans which were in the order of 30 mg/mL. These preliminary results confirm that the part of the studied plant had a very good antimicrobial activity that was proportional to the serial concentrations of the tested extracts.

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