scholarly journals Effect of Archachatina marginata mucin on the aggressive factors of gastric ulcer challenged wistar rat stomach tissue

2019 ◽  
Vol 9 (1) ◽  
pp. 077-082
Author(s):  
Akuma Kalu Amah ◽  
Ogbonnaya Ewa ◽  
Muhammad Rabiu Karimah ◽  
Melford Uche Elendu ◽  
Zaharatu Yunusa
Keyword(s):  
Gastric Ulcer ◽  
Wistar Rat ◽  
Rat Stomach ◽  
Stomach Tissue

scholarly journals Prevention of peptic ulcer by aqueous extract of Aegle marmelos leaf in rats

2018 ◽  
Vol 12 (1) ◽  
pp. 11-14
Author(s):  
Sharmin Rahman ◽  
Mohammad Rezaul Quader ◽  
Md Ismail Khan
Keyword(s):  
Body Weight ◽  
Peptic Ulcer ◽  
Gastric Ulcer ◽  
Aqueous Extract ◽  
Protective Efficacy ◽  
Albino Rats ◽  
Ulcer Formation ◽  
Aegle Marmelos ◽  
Rat Stomach ◽  
Dose Dependent

Background and objectives: Aegle marmelos (Bael), a medicinal plant, has been widely used indigenously to treat many diseases in Bangladesh and other countries. The present study was carried out to evaluate the efficacy of A. marmelos leaf to prevent ethanol induced gastric ulcer in a rat model.Methods: Thirty two Wister albino rats of either sex, weighing between 100-150g, were fed 200 mg/kg or 400 mg/kg aqueous extract of A. marmelos leaves one hour prior to oral administration of 90% ethanol (1 ml/200 gm body weight) to induce gastric ulcer. The animals were sacrificed after one hour and ulcer scores and index were determined. The protective efficacy of A. marmelos aqueous extract was expressed as percentage protection of ulcer.Results: Aqueous extract exhibited significant (p<0.05) dose dependent protection against gastric ulcer formation by ethanol in rat stomach. Percentage protection of ulcer with 200 mg/kg and 400 mg/kg of aqueous extract of A. marmelos leave were 19.3% and 37.2% respectively compared to standard anti-peptic ulcer drug omeprazole (50.4%).Conclusion: Thus, crude extracts of A. marmelos leave have been shown to have potential ability to prevent experimentally induced peptic ulcer formation in animal model.IMC J Med Sci 2018; 12(1): 11-14

Effect of sodium metabisulfite on lipid peroxidation and enzyme activities in adult rat stomach and spleen

2017 ◽  
Vol 7 (1) ◽  
pp. 01-08
Author(s):  
Elkadi Fatima Zohra ◽  
Boufadi Mokhtaria Yasmina ◽  
Meziani Samira ◽  
Sarsar Fatima Zohra ◽  
Djebara Soraya ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Experimental Period ◽  
Wistar Rat ◽  
Redox Status ◽  
Control Group ◽  
Sodium Metabisulfite ◽  
Enzymatic Antioxidants ◽  
Gastric Tissue ◽  
Rat Stomach ◽  
Adult Rat

Sodium metabisulfite (NaMBS) has been used as an antioxidant and antimicrobial agent in a variety of drugs and foods. The aim of our study was to evaluate the effect of subchronic ingestion of NaMBS on lipid peroxidation, protein and enzymatic antioxidants in the gastric tissue and splenic Wistar rat. The rats were divided into four groups (n = 06 rats/group) as follows; Control (C), rats treated with 0.25% NaMBS (S0.25%), rats treated with 1% NaMBS (S1%) and rats treated with 4% NaMBS (S4%). NaMBS was administered to animals via drinking water. At the end of the experimental period, Malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxi-dase (GPx), catalase (CAT) and thiols were determined. Redox status assess-ment revealed that 1% and 4% NaMBS doses induced gastric and spleen lipid peroxidation in animals compared to the control group. On the other hand, activities of SOD, GPx, CAT and thiols were reduced in the S1% and S4% groups in the stomach and spleen compared to the controls. Slight changes were detected in the GPx and CAT in the stomach of S0.25% group compared to the control group. In conclusion, the subchronic consumption of NaMBS has an adverse effect on the spleen and stomach by inducing oxidative damage leading to an increase in lipid peroxidation, protein oxidation and alteration of enzyme activity antioxidant.

scholarly journals Gastroprotective Effect of Aqueous Extract of Azadirachta indica (Neem) on Ethanol Induced Gastric Lesion in Rats

2014 ◽  
Vol 28 (1-2) ◽  
pp. 10-14
Author(s):  
Rifayet Rahman ◽  
Ismail Khan ◽  
Mahbubul Hoque ◽  
Muqbula Tasrin Aktar ◽  
Zinat Rehana Sharmin
Keyword(s):  
Gastric Ulcer ◽  
Aqueous Extract ◽  
Azadirachta Indica ◽  
Gastric Tube ◽  
Gastric Lesion ◽  
Gastric Damage ◽  
Rat Stomach ◽  
Histological Features ◽  
Gastroprotective Effect ◽  
Stomach Damage

The present study has been undertaken to find out the gastro-protective effect of aqueous extract of Azadirachta indica on ethanol induced gastric lesion in rats. To demonstrate this effect : some parameters of gastric damage -such as number of lesions, lesions length, lesions breadth, lesions area, lesions index and percent inhibition was chosen. In addition, histological features of rat stomach were also examined to confirm gastric damage and to determine the extent. The rats were provided with aqueous extract of Azadirachta indica (300mg /kg body wt) orally by gastric tube. After 12 hrs,1 ml absolute ethanol were orally administered to all groups by gastric tube. After 60 mins of administration, all rats were sacrificed. Rats pretreated with aqueous extract of Azadirachta indica (300mg /kg body wt) showed significant decrease in stomach damage both macroscopically and microscopically as compared to control. It is observed that aqueous extract of Azadirachta indica has gastroprotective effect. Therefore, aqueous extract of Azadirachta indica can be used to prevent gastric ulcer. DOI: http://dx.doi.org/10.3329/bjpp.v28i1-2.20074 Bangladesh J Physiol Pharmacol 2012; 28(1&2):10-14

scholarly journals QUERCETIN AND ELLAGIC ACID IN GASTRIC ULCER PREVENTION: AN INTEGRATED SCHEME OF THE POTENTIAL MECHANISMS OF ACTION FROM IN VIVO STUDY

2018 ◽  
Vol 11 (1) ◽  
pp. 381
Author(s):  
Soheir El Sayed Kotob ◽  
Alaa Sayed ◽  
Safaa Mohamed ◽  
Hanaa Ahmed
Keyword(s):  
Gastric Ulcer ◽  
Ellagic Acid ◽  
Healing Process ◽  
Interleukin 2 ◽  
Control Group ◽  
Albino Rats ◽  
Heme Oxygenase 1 ◽  
Tissue Samples ◽  
Stomach Tissue ◽  
Gross Examination

  Objective: The present study was initiated to describe the gastroprotective role of quercetin (Qu) and ellagic acid (EA) on aspirin-induced gastric ulcer (GU) in rats.Methods: Forty adult female albino rats of Wistar strain were distributed into: Control group, GU group, Omeprazole group, Qu group, and EA group. Gross examination, biochemical analyses including serum adrenocorticotropic hormone (ACTH), serotonin (ST), ferritin, heme oxygenase-1 (HO-1), interleukin-2 (IL-2), advanced glycosylation end products (AGEs), and fibronectin (FN) levels were estimated. Moreover, histopathological and histochemical examinations of stomach tissue samples were carried out.Results: Gross examination of gastric mucosa of rats in GU group revealed hyperemia of the stomach mucosa. Furthermore, rats in GU group experienced a significant rise in serum ACTH, ferritin, HO-1, IL-2 and AGEs levels accompanied with significant drop in serum ST and FN levels versus control counterparts. Pre-treatment of GU group with Omeprazole, Qu or EA caused marked improvement in the measured biochemical parameters. Histopathological and histochemical examinations of stomach tissue samples documented the protective action of Omeprazole, Qu and EA with different degrees against GU caused by aspirin.Conclusion: As a conclusion to this study, we can state that both Qu and EA have gastroprotective effect against aspirin-induced GU in rat model. Of note, Qu showed superior impact than EA as an antiulcer agent in this study. The corresponding mechanisms are speculated to be associated with inhibiting stress-induced gastric lesion, attenuating the oxidative stress, iron chelation and blunting ferritin level, modulating inflammatory cascade, and promoting the healing process.

scholarly journals Immunocytochemical Detection of Neuronal Nitric Oxide Synthase (nNOS)-IR in Embryonic Rat Stomach Between Days 13 and 21 of Gestation

2002 ◽  
Vol 50 (5) ◽  
pp. 671-679 ◽  
Author(s):  
Zübeyde Bayram ◽  
Mevlüt Asar ◽  
Sevil çayli ◽  
Ramazan Demir
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Neuronal Nitric Oxide Synthase ◽  
Mesenchymal Cell ◽  
Wistar Rat ◽  
Nerve Fibers ◽  
Nerve Cells ◽  
Rat Stomach ◽  
Glandular Structures ◽  
Oxide Synthase

In this study, the localization and appearance of neuronal nitric oxide synthase-immunoreactive (nNOS-IR) nerve cells and their relationships with the developing gastric layers were studied by immunocytochemistry techniques and light microscopy in embryonic rat stomach. The stomachs of Wistar rat embryos aged 13–21 days were used. The first nerve cells containing nNOS-IR were seen on embryonic Day 14. The occurrence of mesenchymal cell condensation near nNOS-IR neuroblasts on embryonic Day 15 may reflect an active nerve element-specific mesenchymal cell induction causing the morphogenesis of muscle cells. Similarly, the appearance of glandular structures after nNOS-IR neuroblasts, on embryonic Day 18, suggests that the epithelial differentiation may depend on inputs coming from nNOS-IR neuroblasts, as well as other factors. Observation of nNOS-IR nerve fibers on embryonic Day 21 demonstrates that at this stage they contribute to nonadrenergic noncholinergic relaxation. In conclusion, depending on this study's results, it can be said that cells and tissues might be affected by NO secreted by nNOS-IR nerve cells during the development and differentiation of embryonic rat stomach.

scholarly journals QUERCETIN AND ELLAGIC ACID IN GASTRIC ULCER PREVENTION: AN INTEGRATED SCHEME OF THE POTENTIAL MECHANISMS OF ACTION FROM IN VIVO STUDY

2018 ◽  
Vol 11 (1) ◽  
pp. 381
Author(s):  
Soheir El Sayed Kotob ◽  
Alaa Sayed ◽  
Safaa Mohamed ◽  
Hanaa Ahmed
Keyword(s):  
Gastric Ulcer ◽  
Ellagic Acid ◽  
Healing Process ◽  
Interleukin 2 ◽  
Control Group ◽  
Albino Rats ◽  
Heme Oxygenase 1 ◽  
Tissue Samples ◽  
Stomach Tissue ◽  
Gross Examination

  Objective: The present study was initiated to describe the gastroprotective role of quercetin (Qu) and ellagic acid (EA) on aspirin-induced gastric ulcer (GU) in rats.Methods: Forty adult female albino rats of Wistar strain were distributed into: Control group, GU group, Omeprazole group, Qu group, and EA group. Gross examination, biochemical analyses including serum adrenocorticotropic hormone (ACTH), serotonin (ST), ferritin, heme oxygenase-1 (HO-1), interleukin-2 (IL-2), advanced glycosylation end products (AGEs), and fibronectin (FN) levels were estimated. Moreover, histopathological and histochemical examinations of stomach tissue samples were carried out.Results: Gross examination of gastric mucosa of rats in GU group revealed hyperemia of the stomach mucosa. Furthermore, rats in GU group experienced a significant rise in serum ACTH, ferritin, HO-1, IL-2 and AGEs levels accompanied with significant drop in serum ST and FN levels versus control counterparts. Pre-treatment of GU group with Omeprazole, Qu or EA caused marked improvement in the measured biochemical parameters. Histopathological and histochemical examinations of stomach tissue samples documented the protective action of Omeprazole, Qu and EA with different degrees against GU caused by aspirin.Conclusion: As a conclusion to this study, we can state that both Qu and EA have gastroprotective effect against aspirin-induced GU in rat model. Of note, Qu showed superior impact than EA as an antiulcer agent in this study. The corresponding mechanisms are speculated to be associated with inhibiting stress-induced gastric lesion, attenuating the oxidative stress, iron chelation and blunting ferritin level, modulating inflammatory cascade, and promoting the healing process.

A new gastric ulcer model induced by ischemia-reperfusion in the rat: Role of leukocytes on ulceration in rat stomach

Life Sciences ◽  
1996 ◽  
Vol 59 (19) ◽  
pp. PL295-PL301 ◽  
Author(s):  
Kouichirou Wada ◽  
Yoshinori Kamisaki ◽  
Masayuki Kitano ◽  
Yosuke Kishimoto ◽  
Kentaro Nakamoto ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Ischemia Reperfusion ◽  
Rat Stomach ◽  
Ulcer Model

scholarly journals Fabrication of ulcer-adhesive oral keratin hydrogel for gastric ulcer healing in a rat

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Zhongjun Cheng ◽  
Rui Qing ◽  
Shilei Hao ◽  
Yi Ding ◽  
Haimeng Yin ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Ulcer Healing ◽  
High Viscosity ◽  
Rat Stomach ◽  
Gastric Ulcer Healing ◽  
Research Findings ◽  
Endoscopic Guidance ◽  
In Suit ◽  
Extracellular Matrix Scaffold

Abstract Hydrogel has been used for in suit gastric ulcer therapy by stopping bleeding, separating from ulcer from gastric fluids and providing extracellular matrix scaffold for tissue regeneration, however, this treatment guided with endoscopic catheter in most cases. Here, we developed an oral keratin hydrogel to accelerate the ulcer healing without endoscopic guidance, which can specially adhere to the ulcer because of the high-viscosity gel formation on the wound surface in vivo. Approximately 50% of the ulcer-adhesive keratin hydrogel can resident in ethanol-treated rat stomach within 12 h, while approximately 18% of them maintained in health rat stomach in the same amount of time. Furthermore, Keratin hydrogels accelerated the ethanol-induced gastric ulcer healing by stopping the bleeding, preventing the epithelium cells from gastric acid damage, suppressing inflammation and promoting re-epithelization. The oral administration of keratin hydrogel in gastric ulcer treatment can enhance the patient compliance and reduce the gastroscopy complications. Our research findings reveal a promising biomaterial-based approach for treating gastrointestinal ulcers.

Sign in / Sign up

Export Citation Format

Share Document