scholarly journals QUERCETIN AND ELLAGIC ACID IN GASTRIC ULCER PREVENTION: AN INTEGRATED SCHEME OF THE POTENTIAL MECHANISMS OF ACTION FROM IN VIVO STUDY

2018 ◽  
Vol 11 (1) ◽  
pp. 381
Author(s):  
Soheir El Sayed Kotob ◽  
Alaa Sayed ◽  
Safaa Mohamed ◽  
Hanaa Ahmed
Keyword(s):  
Gastric Ulcer ◽  
Ellagic Acid ◽  
Healing Process ◽  
Interleukin 2 ◽  
Control Group ◽  
Albino Rats ◽  
Heme Oxygenase 1 ◽  
Tissue Samples ◽  
Stomach Tissue ◽  
Gross Examination

  Objective: The present study was initiated to describe the gastroprotective role of quercetin (Qu) and ellagic acid (EA) on aspirin-induced gastric ulcer (GU) in rats.Methods: Forty adult female albino rats of Wistar strain were distributed into: Control group, GU group, Omeprazole group, Qu group, and EA group. Gross examination, biochemical analyses including serum adrenocorticotropic hormone (ACTH), serotonin (ST), ferritin, heme oxygenase-1 (HO-1), interleukin-2 (IL-2), advanced glycosylation end products (AGEs), and fibronectin (FN) levels were estimated. Moreover, histopathological and histochemical examinations of stomach tissue samples were carried out.Results: Gross examination of gastric mucosa of rats in GU group revealed hyperemia of the stomach mucosa. Furthermore, rats in GU group experienced a significant rise in serum ACTH, ferritin, HO-1, IL-2 and AGEs levels accompanied with significant drop in serum ST and FN levels versus control counterparts. Pre-treatment of GU group with Omeprazole, Qu or EA caused marked improvement in the measured biochemical parameters. Histopathological and histochemical examinations of stomach tissue samples documented the protective action of Omeprazole, Qu and EA with different degrees against GU caused by aspirin.Conclusion: As a conclusion to this study, we can state that both Qu and EA have gastroprotective effect against aspirin-induced GU in rat model. Of note, Qu showed superior impact than EA as an antiulcer agent in this study. The corresponding mechanisms are speculated to be associated with inhibiting stress-induced gastric lesion, attenuating the oxidative stress, iron chelation and blunting ferritin level, modulating inflammatory cascade, and promoting the healing process.

scholarly journals QUERCETIN AND ELLAGIC ACID IN GASTRIC ULCER PREVENTION: AN INTEGRATED SCHEME OF THE POTENTIAL MECHANISMS OF ACTION FROM IN VIVO STUDY

2018 ◽  
Vol 11 (1) ◽  
pp. 381
Author(s):  
Soheir El Sayed Kotob ◽  
Alaa Sayed ◽  
Safaa Mohamed ◽  
Hanaa Ahmed
Keyword(s):  
Gastric Ulcer ◽  
Ellagic Acid ◽  
Healing Process ◽  
Interleukin 2 ◽  
Control Group ◽  
Albino Rats ◽  
Heme Oxygenase 1 ◽  
Tissue Samples ◽  
Stomach Tissue ◽  
Gross Examination

  Objective: The present study was initiated to describe the gastroprotective role of quercetin (Qu) and ellagic acid (EA) on aspirin-induced gastric ulcer (GU) in rats.Methods: Forty adult female albino rats of Wistar strain were distributed into: Control group, GU group, Omeprazole group, Qu group, and EA group. Gross examination, biochemical analyses including serum adrenocorticotropic hormone (ACTH), serotonin (ST), ferritin, heme oxygenase-1 (HO-1), interleukin-2 (IL-2), advanced glycosylation end products (AGEs), and fibronectin (FN) levels were estimated. Moreover, histopathological and histochemical examinations of stomach tissue samples were carried out.Results: Gross examination of gastric mucosa of rats in GU group revealed hyperemia of the stomach mucosa. Furthermore, rats in GU group experienced a significant rise in serum ACTH, ferritin, HO-1, IL-2 and AGEs levels accompanied with significant drop in serum ST and FN levels versus control counterparts. Pre-treatment of GU group with Omeprazole, Qu or EA caused marked improvement in the measured biochemical parameters. Histopathological and histochemical examinations of stomach tissue samples documented the protective action of Omeprazole, Qu and EA with different degrees against GU caused by aspirin.Conclusion: As a conclusion to this study, we can state that both Qu and EA have gastroprotective effect against aspirin-induced GU in rat model. Of note, Qu showed superior impact than EA as an antiulcer agent in this study. The corresponding mechanisms are speculated to be associated with inhibiting stress-induced gastric lesion, attenuating the oxidative stress, iron chelation and blunting ferritin level, modulating inflammatory cascade, and promoting the healing process.

scholarly journals Ellagic Acid Activity in Healing Process of Incision Wound on Male Albino Rats (Rattus norvegicus)

2017 ◽  
Vol 3 (6) ◽  
pp. 224
Author(s):  
Hardany Primarizky ◽  
Wiwik Misaco Yuniarti ◽  
Bambang Sektiari Lukiswanto
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Ellagic Acid ◽  
Healing Process ◽  
Control Group ◽  
Albino Rats ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Significant Difference ◽  
Incision Wound

This study was conducted to prove whether ellagic acid can be used as a regulator of incision wound healing process in male albino rats as experimental animals. A total of 24 male healthy rats based on physical examination, aged at 3 months old and weight at 150-180 grams used in this study. Rats were divided into three groups and each group has 8 replications, group of control (P0) has no incision wound and no medication, treatment group (P1) has incision wound and treated with Betadine® and (P2) has incision wound and treated with 2,5 % ellagic acid. The variable that observed are histopathology changes (collagen, PMN, angiogenesis and fibrosis) from the male albino rats. The samples collection was taken at day 15 and data were collected and analyzed by Analysis of Variance (ANOVA) test. The result of collagen and PMN cells score in the control group (P0) were 1.80 ± 0.16 and 1.6 ± 0.08 with mild angiogenesis and no fibrosis. The results in the treatment (P2) group, where the collagen and PMN scores were 1.60 ± 0.08 and 1.5 ± 0.8, accompanied by mild angiogenesis and fibrosis. Meanwhile, in the treatment (P1) group, the results of scoring of collagen and PMN were lower than the other groups, 0, 80 ± 0.08 and 0.6 ± 0.16 accompanied by moderate angiogenesis and fibrosis. There was a significant difference of collagen scoring, PMN cells number, fibrosis and angiogenesis among the treatment groups (p<0.05). P1 treatment group has significant different with treatment groups P0 and P2 (p<0.05), but there is no significant difference between treatment groups P0 and P2 (p>0.05).  From the results, it can be concluded that the topical application of ellagic acid ointment was proved to provide healing process on incision wound. Topical administration of ellagic acid ointment for 14 days was able to improve the skin condition and promoted the wound healing.Keywords: Ellagic acid ; wound healing ; collagen ; angiogenesis ; fibrosis ; anti-inflammatory ; antioxidant ; antibacterial.

scholarly journals Impact of Ellagic Acid in Bone Formation after Tooth Extraction: An Experimental Study on Diabetic Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Mazen M. Jamil Al-Obaidi ◽  
Fouad Hussain Al-Bayaty ◽  
Rami Al Batran ◽  
Jamal Hussaini ◽  
Goot Heah Khor
Keyword(s):  
Normal Saline ◽  
Ellagic Acid ◽  
Tooth Extraction ◽  
Healing Process ◽  
Diabetic Rats ◽  
Male Rats ◽  
Diabetic Rat ◽  
Control Group ◽  
Tooth Socket ◽  
The Impact

Objectives. To estimate the impact of ellagic acid (EA) towards healing tooth socket in diabetic animals, after tooth extraction.Methods. Twenty-fourSprague Dawleymale rats weighing 250–300 g were selected for this study. All animals were intraperitoneally injected with 45 mg/kg (b.w.) of freshly prepared streptozotocin (STZ), to induce diabetic mellitus. Then, the animals were anesthetized, and the upper left central incisor was extracted and the whole extracted sockets were filled with Rosuvastatin (RSV). The rats were separated into three groups, comprising 8 rats each. The first group was considered as normal control group and orally treated with normal saline. The second group was regarded as diabetic control group and orally treated with normal saline, whereas the third group comprised diabetic rats, administrated with EA (50 mg/kg) orally. The maxilla tissue stained by eosin and hematoxylin (H&E) was used for histological examinations and immunohistochemical technique. Fibroblast growth factor (FGF-2) and alkaline phosphatase (ALP) were used to evaluate the healing process in the extracted tooth socket by immunohistochemistry test.Results. The reactions of immunohistochemistry for FGF-2 and ALP presented stronger expression, predominantly in EA treated diabetic rat, than the untreated diabetic rat.Conclusion. These findings suggest that the administration of EA combined with RSV may have accelerated the healing process of the tooth socket of diabetic rats, after tooth extraction.

scholarly journals Effects of extractum cepae, heparin, allantoin gel and silver sulfadiazine on burn wound healing: an experimental study in a rat model

2012 ◽  
Vol 57 (No. 6) ◽  
pp. 287-292 ◽  
Author(s):  
AS Durmus ◽  
M. Yaman ◽  
HN Can
Keyword(s):  
Wound Healing ◽  
Rat Model ◽  
Silver Sulfadiazine ◽  
Control Group ◽  
Albino Rats ◽  
Burn Wound ◽  
Tissue Samples ◽  
Burned Areas ◽  
Positive Effects ◽  
Burn Wound Healing

These experiments were conducted in order to compare the effects of extractum cepae, heparin, allantoin gel (CTBX) and silver sulfadiazine (SSD) cream on burn wound healing in rats. Thirty six adult, female Wistar albino rats were divided into three equal groups. A burn was made on the back of all rats. The burned areas in the first, second and third groups were covered with cold cream (control), SSD skin cream and CTBX twice a day, respectively. Seven and 14 days later, the rats were sacrificed and burned skin tissue samples were collected from the rats for histopathological examinations. Histopatological evaluations on the 7<sup>th</sup> and 14<sup>th</sup> days showed burn healing to be better in the CTBX and SSD groups with respect to the control group. The best burn wound healing was observed in the CTBX group (P &lt; 0.001). Wound healing was significantly different between the groups at days 7 and 14 (P &lt; 0.001). In conclusion, application of CTBX has significant positive effects on the healing of burn wounds in a rat model. &nbsp;

scholarly journals The Potential Role of Hemopexin and Heme Oxygenase-1 Inducer in a Model of Sepsis

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Passainte S. Hassaan ◽  
Radwa A. Mehanna ◽  
Abeer E. Dief
Keyword(s):  
Severe Sepsis ◽  
Heme Oxygenase ◽  
Histopathological Examination ◽  
Survival Rates ◽  
Cecal Ligation ◽  
Lung Parenchyma ◽  
Control Group ◽  
Albino Rats ◽  
Heme Oxygenase 1 ◽  
Sepsis Induction

Background and Aims. Sepsis can evoke disseminated intravascular coagulation, resulting in multiple organ failure and death. Heme oxygenase-1 (HO-1) and hemopexin (HPx) can mediate cytoprotective mechanisms against these deleterious effects. This study aims to determine a role for HO-1 and HPx in coagulopathy induced by septic inflammation and define whether they can enhance the production of anti-inflammatory cytokine IL-10. Materials and Methods. 48 healthy male albino rats were divided equally into 4 groups: control group: animals subjected to laparotomy and bowel manipulation; CLP group: severe sepsis induced by cecal ligation puncture (CLP); CLP + hemin group: animals received single intraperitoneal injection of hemin (50 µmol/kg) 12 h before sepsis induction; CLP + HPx group: animals received single HPx dose (150 µg/rat, i.v.) 30 min before sepsis induction. Survival rates were calculated. Prothrombin time (PT), activated partial thromboplastin time (APTT), and activated protein C (APC), liver HO-1, serum, and liver IL-10 levels were measured, 48 hrs after sepsis induction. Liver and lung were excised for histopathological examination. Results. Hemin and HPx administration upregulated liver HO-1 and IL-10. They prolonged PT, PTT and increased APC. They reduced the inflammatory infiltrate and thrombosis in liver and lung parenchyma. However, hemin was superior in controlling coagulopathy and HO-1 production, while HPx was more potent stimulant of IL-10 expression. Conclusions. Hemin and HPx have a potential beneficial effect in severe sepsis regarding coagulopathy and inflammation.

scholarly journals Investigating the healing effect of the hydroalcoholic extract of pomegranate seed (Punica granatum) on the full thickness wound in rabbit

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Ali Asghar Hemmati ◽  
Iran Rashidi ◽  
Sedigheh Dahanzadeh ◽  
Mahmoud Moeini
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Punica Granatum ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Hydroxyproline Content ◽  
Tissue Samples ◽  
Treatment Groups ◽  
Wound Recovery

Objective: This research designed to investigate the wound healing process with pomegranate hydroalcoholic seed extract (Punica granatum) in comparison with no-treatment, betamethasone, phenytoin and eucerin in rabbits. Methods: The positive group including groups that received phenytoin cream (1%) and topical eucerin, respectively, twice a day to complete wound healing. negative Control group did not obtain any treatment. Treatment groups were received cream of PSE (2, 5,7,10 w/w) in eucerin and 75% w/w as purified extract twice daily. In order to measure the percentage of wound healing, the zone of the wound was evaluated daily. Histological studies were done on the 7th and 15th days of treatment. Next, hydroxyproline content of wounds healed and tensile strength of wound tissue samples were measured. Results: The results demonstrated between PSE treatment groups and eucerin animals were statistically significant aadifferences (P<0.05) in most of the days reviewed. Treatment of Rabbits with 10% PSE had the best results (complete wound recovery in 12 days). Also, this treatment showed higher hydroxyproline content and higher tissue strength. Conclusion: This research reveals that the extract of 10% PSE administrated topically has the proper potential to induce wound recovery in the wound model of rabbits. In addition, 10% PSE accelerates the healing of the wound. Further study needs to clarify the results of this research.

scholarly journals Autologous omentum transposition for regeneration of a renal injury model in rats

2022 ◽  
Vol 9 (1) ◽  
Author(s):  
Tayfun Bilgiç ◽  
Ümit İnce ◽  
Fehmi Narter
Keyword(s):  
Foreign Body ◽  
Foreign Body Reaction ◽  
Primary Repair ◽  
Healing Process ◽  
Kidney Injury ◽  
Control Group ◽  
Albino Rats ◽  
Loss Of Function ◽  
Omentum Transposition ◽  
Process Completion

Abstract Background After renal trauma, surgical treatment is vital, but sometimes there may be loss of function due to fibrosis. This study aimed to evaluate the effect of autologous omentum flaps on injured renal tissues in a rat model. Methods A total of 30 Wistar albino rats were included and randomly divided equally into a control group and four intervention groups. Iatrogenic renal injuries were repaired using a surgical technique (primary repair 1 group and primary repair 2 group) or transposition of the autologous omentum (omentum repair 1 group and omentum repair 2 group). Blood samples were taken preoperatively and on the 1st and 7th postoperative days in all groups and on the 18th postoperative day in the control and two intervention groups. All rats were sacrificed on the 7th or 18th day postoperatively, and their right kidneys were taken for histopathological evaluation. Results The mean urea level significantly decreased from day 1 to day 7 and from day 1 to day 18 in the omentum repair 2 group (P = 0.005 and P = 0.004, respectively). There were no other significant changes in urea or creatinine levels within the intervention groups (P > 0.05). There was no significant correlation between the urea and creatinine levels and the histological scores (P > 0.05). The primary repair 1 and 2 groups had significantly higher median granulation and inflammation scores in the kidney specimen than the control and omentum repair groups (P < 0.05). The omentum repair 2 group had significantly lower median granulation and inflammation scores in the surrounding tissues than the primary repair 2 group (P < 0.05). The completion score for the healing process in the kidney specimen was significantly higher in the omentum repair groups than in the primary repair groups (P < 0.05). The omentum repair 2 group had significantly lower median granulation and inflammation scores in the surrounding tissues than the primary repair 2 group (P < 0.05). Granulation degree in the kidney specimen was strongly and positively correlated with the inflammation degree (r = 0.824, P < 0.001) and foreign body reaction in the kidney specimen (r = 0.872, P < 0.001) and a strong and negative correlation with the healing process completion score in the kidney (r = − 0.627, P = 0.001). Inflammation degree in the kidney specimen was strongly and positively correlated with the foreign body reaction in the kidney specimen (r = 0.731, P = 0.001) and strongly and negatively correlated with the healing process completion score in the kidney specimen (r = − 0.608, P = 0.002). Conclusion Autologous omentum tissue for kidney injury repair attenuated inflammation and granulation. Additionally, the use of omental tissue to facilitate healing of kidney injury may theoretically lead to a more effective healing process and reduced fibrosis and tissue and function loss.

Redox and apoptotic potential of novel ruthenium complexes in the rat blood and heart

2020 ◽  
Author(s):  
Katarina Mihajlovic ◽  
Isidora Milosavljevic ◽  
Jovana Jeremic ◽  
Maja Savic ◽  
Jasmina Sretenovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Caspase 3 ◽  
Ruthenium Complexes ◽  
Redox Status ◽  
Heart Tissue ◽  
Control Group ◽  
Albino Rats ◽  
Oxidative Stress Biomarkers ◽  
Tissue Samples ◽  
Relative Expression

Ruthenium(II) complexes offer the potential for lower toxicity compared to platinum(II) complexes. Our study aimed to compare cardiotoxicity of [Ru(Cl-tpy)(en)Cl][Cl], [Ru(Cl-tpy)(dach)Cl][Cl], [Ru(Cl-tpy)(bpy)Cl][Cl], cisplatin and saline through assessment of redox status and relative expression of apoptosis-related genes. A total of 40 Wistar albino rats were divided into five groups. Ruthenium groups received intraperitoneally single dose of complexes (4 mg/kg/week) for 4-weeks period; cisplatin group received cisplatin (4 mg/kg/week) and control group received saline (4 mL/kg/week) in the same manner as ruthenium groups. In collected blood and heart tissue samples, spectrophotometrically determination of oxidative stress biomarkers was performed. The relative expression of apoptosis-related genes (Bcl-2, Bax, and caspase-3) in hearts was examined by RT-PCR. Our results showed that systemic and cardiac pro-oxidative markers (TBARS and NO2-) were significantly lower in ruthenium groups compared to cisplatin group, while concentrations of antioxidative parameters (CAT, SOD, and GSSG) were significantly higher. Ruthenium administration led to significantly lower gene expression of Bax and caspase-3 compared to cisplatin-treated rats, while Bcl-2 remained unchanged. Applied ruthenium complexes have less pronounced potential for induction of oxidative stress-mediated cardiotoxicity compared to cisplatin. These findings may help for future studies that should clarify the mechanisms of cardiotoxicity of ruthenium-based metallodrugs.

scholarly journals The Response of the Pulp-Dentine Complex, PDL, and Bone to Three Calcium Silicate-Based Cements: A Histological Study in an Animal Rat Model

2020 ◽  
Vol 2020 ◽  
pp. 1-13 ◽  
Author(s):  
Ranjdar Mahmood Talabani ◽  
Balkees Taha Garib ◽  
Reza Masaeli
Keyword(s):  
Inflammatory Response ◽  
Calcium Silicate ◽  
Histological Study ◽  
Healing Process ◽  
Mineral Trioxide Aggregate ◽  
Experimental Period ◽  
Tissue Reaction ◽  
Cortical Plate ◽  
Control Group ◽  
Albino Rats

Objective. The aim of this study was to histologically examine the tissue reaction of three different calcium silicate cements in the closure of perforations in rat incisor teeth. Material and Methods. An experimental lateral root perforation with pulp exposure was performed in 32 lower incisors of 16 male Wistar albino rats. They were randomly assigned into three test groups (each including eight teeth) that were filled either by Biodentine (BD) or MicroMega mineral trioxide aggregate (MM-MTA) or EndoSequence root repair material putty (ESRRM putty), besides eight unperforated incisors from the other four rats (control group). The inflammatory response and healing process were evaluated histologically and scored after one and four weeks. Differences among groups were tested by Kruskal–Wallis tests at P≤0.05. Results. In the first week, BD produced more inflammatory response in the pulpal (score 3) than other materials (score 2). Only ESRRM putty showed odontoblast-like cells in 50%, 25% dentine-like deposit, 25% evidence of bone deposition in the drilling site (score 2), and minimum periodontal ligament (PDL) necrosis and disorganization (25%, score 2). After one month, all groups had healthy pulpal tissue, but 25% of ESRRM putty retained score 1 inflammatory response, and 50% of the BD case had an incomplete palisading odontoblast layer (score 3). A thick and regular dentine bridge deposition was seen in the ESRRM putty group in comparison with MM-MTA and BD cases. The cortical plate healing in all ESRRM putty samples was complete (score 3), while an incomplete closure was seen in MM-MTA and BD groups (score 2). Both the MM-MTA and ESRRM putty groups had fully organized PDL (score 2), while in 50% of BD cases, a necrotizing area and disorganized PDL with inflammatory cells infiltration were still present. Statistically significant differences in the scores of any histologic parameters among the three tested materials were observed neither in the 1st nor in the 4th weeks of the experimental period. Conclusion. Better tissue compatibility and repair of pulpal and periodontal tissue have been detected after lateral perforation in the root of rat incisors when treated with ESRRM putty than MM-MTA and BD. However, the difference was not significant.

Pregabalin Ameliorates Lipopolysaccharide-Induced Pancreatic Inflammation in Aged Rats

2019 ◽  
Vol 19 (8) ◽  
pp. 1141-1147 ◽  
Author(s):  
Ozlem Ozmen ◽  
Senay Topsakal
Keyword(s):  
Caspase 3 ◽  
Pancreatic Tissue ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Aged Rats ◽  
Tissue Samples ◽  
Glucose Levels ◽  
Amyloid A ◽  
Group I

Objective: The aim of this study was to examine pancreatic pathology and the prophylactic effects of pregabalin in lipopolysaccharide (LPS) induced sepsis model in aged rats. Methods: Twenty-four female, one-year-old, Wistar Albino rats were assigned to three groups; Group I (control), Group II (study group: 5mg/kg LPS intraperitoneal, single dose) and Group III(treatment group: 5mg/kg LPS+30 mg/kg oral pregabalin one hour before LPS). Animals were sacrificed by exsanguination 6 hours after LPS administration. Blood and pancreatic tissue samples were collected for biochemical, pathological, and immunohistochemical analyses. Results: LPS caused increases in serum amylase and lipase level but led to a reduction in glucose levels. Following histopathological analysis, numerous neutrophil leucocyte infiltrations were observed in vessels and pancreatic tissues. Increased caspase-3 expression was observed in both the endocrine and exocrine pancreas in the LPS group. Similarly, IL-6, caspase-3 (Cas-3), inducible nitric oxide synthase (iNOS), granulocyte colony-stimulating factor (G-CSF) and serum amyloid-A (SAA) expressions were increased by LPS. Pregabalin improved biochemical, histopathological, and immunohistochemical findings. Conclusion: This study showed that LPS causes pathological findings in the pancreas, but pregabalin has ameliorative effects in aged rats with sepsis. Cas-3, IL-6, iNOS, G-CSF, and SAA all play pivotal roles in the pathogenesis of LPS-induced pancreatic damage.

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