scholarly journals Prevention of doxorubicin-induce renal function abnormalities by turmeric in Wistar rats

2021 ◽  
Vol 14 (3) ◽  
pp. 143-156
Author(s):  
Isirima Joshua Charles ◽  
Okoroafor Dorcas Okayo
Keyword(s):  
Renal Function ◽  
Vitamin E ◽  
Vitamin C ◽  
Wistar Rats ◽  
Serum Levels ◽  
Saline Group ◽  
Negative Control ◽  
Wide Range ◽  
Group 5 ◽  
Doxorubicin Group

Doxorubicin is an anthracycline antibiotic with anti-neoplastic potentials mediated mainly by affecting the DNA synthetic machinery of the cell. It is found to be effective against a wide range of cancers including the ovary, uterus, lung, breast etc. Doxorubicin exerts its nephrotoxicity on the kidney by inducing inflammatory changes leading to increased capillary porousness and glomerular shrinking. This study investigated the effect of turmeric on the renal function biomarkers in doxorubicin-induced oxidative stress in Wistar rats. 54 adult Wistar rats were divided into 9 groups of six animals each. Group 1 animals served as control (normal saline), group 2 animals served as negative control, and received doxorubicin, group 3 animals were given doxorubicin and turmeric, group 4 animals received doxorubicin and vitamin C, group 5 animals received doxorubicin and vitamin E, group 6 animals received doxorubicin, vitamins C and turmeric, group 7 animals received doxorubicin, vitamin E and turmeric, while group 8 animals received doxorubicin, vitamin C and vitamin E and finally, group 9 animals receive doxorubicin, vitamin C, vitamin E and turmeric. The experiment lasted for 28 days and blood samples were collected from each animal from the various groups for renal function assay. Doxorubicin caused significant increase in the serum levels of urea, creatinine, sodium (Na+), calcium (Ca2+), and decrease serum levels of chloride (Cl-), magnesium (Mg2+) and potassium (K+). These abnormal electrolyte imbalances were prevented by administration of turmeric alone or in combination with vitamins C and or E along with doxorubicin concomitantly.

scholarly journals Prevention of doxorubicin-induced haematotoxicity by turmeric in Wistar rats

2021 ◽  
Vol 9 (3) ◽  
pp. 096-108
Author(s):  
Joshua Charles Isirima ◽  
Dorcas Okayo Okoroafor
Keyword(s):  
Bone Marrow ◽  
Vitamin E ◽  
Vitamin C ◽  
Blood Cell ◽  
Cell Count ◽  
Wistar Rats ◽  
Soft Tissue Sarcomas ◽  
Negative Control ◽  
Monocyte Count ◽  
Blood Cell Count

Doxorubicin is a known anticancer drug used in treatment of various cancers including leukemias, lymphomas, soft-tissue sarcomas and solid tumors. Doxorubicin exerts its toxicity on the blood by causing bone marrow suppression, myelosuppression, pronounced marrow depression and bone marrow depletion, and hypoplasia This study investigated the effect of turmeric on the haematological parameters in doxorubicin-induced oxidative stress in Wistar rats. 54 adult Wistar rats were divided into 9 groups of six animals each. Group 1 animals served as control (normal saline), group 2 animals served as negative control, and received doxorubicin, group 3 animals were given doxorubicin and turmeric, group 4 animals received doxorubicin and vitamin C, group 5 animals received doxorubicin and vitamin E, group 6 animals received doxorubicin, vitamins C and turmeric, group 7 animals received doxorubicin, vitamin E and turmeric, while group 8 animals received doxorubicin, vitamin C and vitamin E and finally, group 9 animals receive doxorubicin, vitamin C, vitamin E and turmeric. The experiment lasted for 28 days and blood samples were collected from each animal from the various groups for haematological assay. Doxorubicin caused a significant decrease in the serum levels of white blood cell count, neutrophil count, lymphocyte count, monocyte count, platelet count, red blood cell count, hemoglobin concentration, hematocrit and mean corpuscular volume. These changes were prevented by turmeric alone or in combined concomitant administration with vitamins C and or E and doxorubicin

scholarly journals Kidney ischemia and reperfunsion syndrome: effect of lidocaine and local postconditioning

2016 ◽  
Vol 43 (5) ◽  
pp. 348-353 ◽  
Author(s):  
IGOR NAGAI YAMAKI ◽  
RUY VICTOR SIMÕES PONTES ◽  
FELIPE LOBATO DA SILVA COSTA ◽  
VITOR NAGAI YAMAKI ◽  
RENAN KLEBER COSTA TEIXEIRA ◽  
...  
Keyword(s):  
Ischemia Reperfusion ◽  
Serum Levels ◽  
Saline Group ◽  
Renal Ischemia ◽  
Ischemic Postconditioning ◽  
Control Group ◽  
Ischemia And Reperfusion ◽  
Lidocaine Group ◽  
Group 5 ◽  
Group 2

ABSTRACT Objective: to evaluate the effects of blocking the regulation of vascular tone on the ischemia and reperfusion syndrome in rats through the use of lidocaine in the postconditioning technique. Methods: we randomized 35 rats into seven groups of five animals: Group 1- Control; Group 2- Ischemia and Reperfusion; Group 3- Ischemia, Reperfusion and Saline; Group 4- Ischemic Postconditioning; Group 5- Ischemic Postconditioning and Saline; Group 6- Lidocaine; Group 7- Ischemic Postconditioning and Lidocaine. Except for the control group, all the others were submitted to renal ischemia for 30 minutes. In postconditioning groups, we performed ischemia and reperfusion cycles of five minutes each, applied right after the main ischemia. In saline and lidocaine groups, we instilled the substances at a rate of two drops per minute. To compare the groups, we measured serum levels of urea and creatinine and also held renal histopathology. Results: The postconditioning and postconditioning + lidocaine groups showed a decrease in urea and creatinine values. The lidocaine group showed only a reduction in creatinine values. In histopathology, only the groups submitted to ischemic postconditioning had decreased degree of tubular necrosis. Conclusion: Lidocaine did not block the effects of postconditioning on renal ischemia reperfusion syndrome, and conferred better glomerular protection when applied in conjunction with ischemic postconditioning.

scholarly journals Effectiveness of Vitamin C and Vitamin E Antioxidant Combination on Caspase-3 Expression in Wistar White Rat Pulmonum Contusion

2020 ◽  
Vol 3 (1) ◽  
pp. 31-44
Author(s):  
Bermansyah ◽  
Gama Satria ◽  
Ahmad Umar
Keyword(s):  
Cell Death ◽  
Vitamin E ◽  
Vitamin C ◽  
Wistar Rats ◽  
Caspase 3 ◽  
Cell Function ◽  
Pulmonary Contusion ◽  
Tnf Α ◽  
Male Wistar Rats

Introduction.Pulmonary contusions can cause a progressive inflammatory response. Activation of TNF-α cytokines and reactive oxygen species (ROS) can cause pulmonary cell death. Antioxidants can have the potential to neutralize ROS. The purpose of this study is to determine the effectiveness of antioxidant administration in maintaining pulmonary cell function in wistar rats that have been induced to experience pulmonary contusions through caspase-3 levels. Methods.This study was an in vivo experimental study conducted on thirty male wistar rats and divided into five groups (n = 6): control, pulmonary contusion + asthaxanthine 5 mg/kgBW, pulmonary contusion + vitamin C and E 50 mg/kgBW, pulmonary contusion + vitamin C and E 100 mg/kgBW, pulmonary contusion + vitamin C and E 200 mg/kgBW. The value of Caspase-3 is evaluated by the IHC. All data analyzes used SPSS 18. Results. Low doses of antioxidants have the potential to reduce pulmonary cell death in wistar rats induced by pulmonary contusions.Conclussion. Vitamin C and E effective to reduce polmonary cell death in pulmonary contusion.Keywords: antioxidants, vitamin C, vitamin E, pulmonary contusions animal model, apoptosis, caspase-3

Toxicity of bean cooking media containing EDTA in mice

2020 ◽  
Vol 36 (6) ◽  
pp. 436-445
Author(s):  
Sabry Ali El-Naggar ◽  
Karim Samy El-Said ◽  
Mona Elwan ◽  
Maysa Mobasher ◽  
Fotouh Mansour ◽  
...  
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
White Blood Cells ◽  
Saline Group ◽  
Negative Control ◽  
Blood Levels ◽  
Group 4 ◽  
Liver And Kidney ◽  
Group 5 ◽  
Group 2 ◽  
Kidney Functions

The possible renal and hepatic toxicities of ethylenediaminetetraacetic acid (EDTA) in bean cooking media were studied using 100 male albino mice. Two sublethal doses of EDTA were used to explore their toxic effects; 20 mg/kg and 200 mg/kg, which corresponded to 1/100th and 1/10th of LD50, respectively. Accordingly, the toxicity study was performed using 50 mice, divided into five groups ( n = 10/group) as follows: group 1 (Gp1) served as a negative control and was orally administered normal saline; group 2 (Gp2) was administered the bean cooking medium; group 3 (Gp3) was administered EDTA (200 mg/kg); group 4 (Gp4) was administered bean cooking medium containing 20 mg/kg of EDTA; and group 5 (Gp5) was administered bean cooking medium containing 200 mg/kg of EDTA. The results showed no significant changes in liver and kidney functions in Gp2 while Gp3, Gp4, and Gp5 exhibited significant increases in adverse liver and kidney function markers. Hematocrit values were significantly decreased in Gp3 and Gp5, while the total white blood cells counts were significantly decreased in Gp3 and significantly increased in Gp5. The number of platelets was decreased in Gp3, Gp4, and Gp5. The blood levels of sodium (Na+), iron (Fe2+), and calcium (Ca2+) were decreased in Gp3, Gp4, and Gp5 due to the chelating effects of EDTA. The hepatic and renal architectures were disorganized in Gp3, Gp4, and Gp5 with some hemorrhagic manifestations in livers and kidneys of mice. These results demonstrate that EDTA in bean cooking is harmful in mice under the conditions of this study, and the potentially harmful effects in humans supports restricting its use.

scholarly journals Vitamin E, Vitamin C, or Losartan Is Not Nephroprotectant against Cisplatin-Induced Nephrotoxicity in Presence of Estrogen in Ovariectomized Rat Model

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Mehdi Nematbakhsh ◽  
Zahra Pezeshki ◽  
Fatemeh Eshraghi-Jazi ◽  
Farzaneh Ashrafi ◽  
Hamid Nasri ◽  
...  
Keyword(s):  
Vitamin E ◽  
Vitamin C ◽  
Kidney Tissue ◽  
Serum Levels ◽  
Ovariectomized Rats ◽  
Estradiol Valerate ◽  
Group 4 ◽  
Vitamins E And C ◽  
Losartan Group ◽  
Group 2

Background. The nephroprotective effect of vitamins E and C or losartan against cisplatin (CP)- induced nephrotoxicity when they are accompanied by estrogen was investigated.Methods. The ovariectomized rats received estradiol valerate for two weeks. At the end of the first week, a single dose of CP (7 mg/kg, IP) was also administered, and they received placebo (group 1), vitamin E (group 2), vitamin C (group 3), or losartan (group 4) every day during the second week, and they were compared with another three control groups.Results. CP alone increased the serum levels of blood urea nitrogen (BUN), creatinine (Cr), and kidney tissue damage score (KTDS), significantly (P<0.05), however at the presence of estradiol and CP, vitamin C, vitamin E, or losartan not only did not decrease these parameters, but also increased them significantly (P<0.05). The serum level of superoxidase dismutase (SOD) was reduced by CP (P<0.05), but it was increased when estradiol or estradiol plus vitamin C or losartan were added (P<0.05).Conclusion. The particular pharmacological dose of estrogen used in this study abolish the nephroprotective effects vitamins C and E or losartan against CP-induced nephrotoxicity.

scholarly journals Antimalarial combination therapies increase gastric ulcers through an imbalance of basic antioxidative-oxidative enzymes in male Wistar rats

2020 ◽  
Author(s):  
Muhamudu Kalange ◽  
Miriam Nansunga ◽  
Keneth Iceland Kasozi ◽  
Josephine Kasolo ◽  
Jackline Namuleme ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Wistar Rats ◽  
Saline Group ◽  
Positive Control ◽  
Negative Control ◽  
Oxidative Enzymes ◽  
Gastric Ulcers ◽  
Combination Therapies ◽  
Male Wistar Rats ◽  
High Gastric Ulcer

Abstract Objective: Antimalarials are globally used against plasmodium infections, however, information on the safety of new antimalarial combination therapies on the gastric mucosa is scarce. The aim of the study was to establish the effects of Artesunate-Amodiaquine and Artemether-Lumefantrine on gastric ulcers, malondialdehyde (MDA), reduced glutathione (GSH) and identify major histological changes in male Wistar rats. Gastric ulcers were induced using Indomethacin in four groups and group 1 was administered Artesunate, group 2 received Artesunate-Amodiaquine, group 3 received Artemether-Lumefantrine, and group 4 was a positive control (normal saline). Group five was the negative control consisting of healthy rats. Results: Antimalarial combination therapies were associated with a high gastric ulcer index than a single antimalarial agent, Artesunate. In addition, levels of MDA were significantly higher in the combination of therapies while levels of GSH were lower in comparison to Artesunate and the negative control. Microscopically, antimalarial combination therapies were associated with severe inflammation and tissue damage than Artesunate in the gastric mucosa showing that antimalarial combination therapies exert their toxic effects through oxidative stress mechanisms, and this leads to apoptosis. Findings in this study demonstrate a new to revisit information on the pharmacodynamics of major circulating antimalarial agents in developing countries.

scholarly journals Immunomodulatory and Anti-Inflammatory Potentials of Trigona Honey in the Therapy and Prevention against Respiratory Infection in Wistar Rats

2020 ◽  
Vol 11 (3) ◽  
pp. 2955-2962
Author(s):  
Ibrahim Khaled Al-kafaween ◽  
Abu Bakar Mohd Hilmi ◽  
Mohamed M. Soliman
Keyword(s):  
Respiratory Infection ◽  
Wistar Rats ◽  
Serum Levels ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Gene Expressions ◽  
Anti Inflammatory

Trigona honey (TH) is well known for its therapeutic characteristics. To date, the study of Trigona honey as a prophylactic or immune booster prior to the bacterial infection of the invivo model is not well covered. This study aims to investigate anti-inflammatory and immune activities in Wistar rats infected with respiratory infection following with Trigona honey. 25 Wistar rats were assigned to possitive groups, negative control group, positive control group was fed TH (5 g / kg body weight) orally, the untreated group was infected with Staphylococcus aureus to induce respiratory infection, the treated group has been infected with S. aureus followed by treatment with TH at a dose of 1.5 ×108 CFU / mL and the preventive group ingested TH one week before S. aureus infection. Blood was obtained for biochemical analysis. Lung tissues have been collected for molecular examination. The results showed a significant decrease in serum levels of ALT, AST, urea and creatinine in the preventive and treated groups, serum IgG increased significantly (P<0.05) in the preventive and treated groups, IFN-y increased in the preventive group while decreased in the treated group, and IL-8 increased in the treated group while decreased in the preventive group. The mRNA expression of AGP is up-regulated in the positive control, preventive and treated groups. The α2-MG, TNF-α , and mRNA expressions showed lower regulation after administration of TH in preventive and treated groups. The results show the ability of TH to counteract immune and inflammatory changes in serum levels and gene expressions.

scholarly journals Combination of vitamin E and vitamin C alleviates renal function in hyperoxaluric rats via antioxidant activity

2017 ◽  
Vol 79 (5) ◽  
pp. 896-903 ◽  
Author(s):  
Orapun JATURAKAN ◽  
Thasinas DISSAYABUTRA ◽  
Narongsak CHAIYABUTR ◽  
Anusak KIJTAWORNRAT ◽  
Piyaratana TOSUKHOWONG ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Renal Function ◽  
Vitamin E ◽  
Vitamin C

scholarly journals Endocrine and Neurobehavioral Abnormalities Induced by Propofol Administered to Neonatal Rats

2014 ◽  
Vol 121 (5) ◽  
pp. 1010-1017 ◽  
Author(s):  
Sijie Tan ◽  
Changqing Xu ◽  
Wanting Zhu ◽  
Jesse Willis ◽  
Christoph N. Seubert ◽  
...  
Keyword(s):  
Prepulse Inhibition ◽  
Acoustic Startle ◽  
Serum Levels ◽  
Saline Group ◽  
Negative Control ◽  
Female Rats ◽  
Sprague Dawley ◽  
Ca1 Neurons ◽  
Male And Female Rats ◽  
Acid Type

Abstract Background: The authors studied whether neonatal propofol anesthesia affects development of the endocrine and neural systems. Methods: Sprague–Dawley rats were anesthetized using intraperitoneal propofol for 5 h on postnatal days (P) 4, 5, or 6. Pups that received either saline or intralipid, but not those in the negative control groups, were also maternally separated for 5 h. Serum levels of corticosterone were measured immediately after anesthesia and in adulthood after prepulse inhibition of acoustic startle testing (≥P80), followed by measurement of hippocampal neuronal activity. Results: Propofol acutely increased corticosterone levels to 146.6 ± 23.5 ng/ml (n = 6) versus 16.4 ± 3.5 ng/ml (n = 6) and 18.4 ± 3.2 ng/ml (n = 6) in saline- and intralipd-treated pups, respectively. In adulthood, the propofol group exhibited exacerbated endocrine responses to stress in a form of increased corticosterone levels (1,171.58 ± 149.17 ng/ml [n = 15] vs. 370.02 ± 36.01 ng/ml [n = 10] in the saline group). The propofol group had increased the frequency of miniature inhibitory postsynaptic currents in CA1 neurons of male and female rats, but reduced prepulse inhibition of startle was detected only in males. The Na+–K+–2Cl− cotransporter inhibitor bumetanide, administered to pups before propofol injection, alleviated long-term endocrine and prepulse inhibition abnormalities. Exogenous corticosterone, administered to naive pups, induced synaptic and endocrine but not prepulse inhibition effects, similar to those of propofol. Conclusion: Propofol-caused acute increases in corticosterone levels and γ-aminobutyric acid type A receptor–mediated excitation at the time of anesthesia may play mechanistic roles in development of exacerbated endocrine responses to stress and neurobehavioral abnormalities.

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