scholarly journals Anti-microbial resistant in Gram-negative bacteria bacillus: Enterobacteriaceae and non-fermenting bacilli isolated at Sominé DOLO Hospital of Mopti, Mali

2022 ◽  
Vol 18 (1) ◽  
pp. 008-013
Author(s):  
Moussa Diawara ◽  
Modibo Coulibaly ◽  
Dramane Samaké ◽  
Soumaila Touré ◽  
Dramane Cissé ◽  
...  
Keyword(s):  
Hospital Setting ◽  
Cross Sectional Study ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Sensitivity Testing ◽  
Cross Sectional ◽  
Gram Negative ◽  
Epidemiological Aspect ◽  
Antibiotics Sensitivity ◽  
High Level

Background: β-lactams and carbapenems. are the major antibiotics used to treat gram-negative bacteria and non-fermenting bacilli. However, the increasing production of β-lactamase and carbapenemase limits the therapeutic options. Our study aims to determine the resistant phenotypes of these bacteria while describing their epidemiological aspect. Material and Methods: This was a cross-sectional study by consecutive enrollment from January 2018 to December 2019 at Sominé DOLO Hospital of Mopti, Mali. We performed manual method for bacteria culture, identification and antibiotics sensitivity testing. The antibiotics sensitivity testing was accessed by the diffusion method according to CA-SFM/EUCAST (“Comité de l’Antibiogramme de la Société Française de Microbiologie” / European Committee on Antimicrobial Susceptibility Testing) recommendations V1.0 february 2018 and V2.0 may 2019 2019 V.2.0. May recommendation released in 2019. Data were analyzed by software R 4.0.3 GUI 1.73 Catalina build (7892). Results: At all 904 samples were included in this study. Out of the 904 cultures, 297 sample (32.85%) were positive. The rates of enzymes production were as follow: Extended-spectrum ß-lactamase (ESBL) 56.42% (101/179), cepholosporinase hyperproduction (HCASE) 15.64% (28/179), cephalosporinase production (CASE) 6.14% (11/179), penicillinase hyperproduction (HP) 5.58% (10/179), carbapenemase production (CP) 6.14% (11/179) and savage strains 10.05% (18/179). Conclusion: Our data showed a high prevalence of resistance to β-lactamins ß-talactamins and carbapenemes in gram-negative bacteria and non-fermenting bacillus bacilli. The A high level of β-lactamase and carbapenemase production by gram negative bacillus were also reported by others authors calls for the rational use of antibiotic in hospital setting.

scholarly journals Anti-microbial resistant in Gram-negative bacteria bacillus: Enterobacteriaceae and non-fermenting bacilli isolated at Sominé DOLO Hospital of Mopti, Mali

2022 ◽  
Vol 18 (1) ◽  
pp. 008-013
Author(s):  
Moussa Diawara ◽  
Modibo Coulibaly ◽  
Dramane Samaké ◽  
Soumaila Touré ◽  
Dramane Cissé ◽  
...  
Keyword(s):  
Hospital Setting ◽  
Cross Sectional Study ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Sensitivity Testing ◽  
Cross Sectional ◽  
Gram Negative ◽  
Epidemiological Aspect ◽  
Antibiotics Sensitivity ◽  
High Level

Background: β-lactams and carbapenems. are the major antibiotics used to treat gram-negative bacteria and non-fermenting bacilli. However, the increasing production of β-lactamase and carbapenemase limits the therapeutic options. Our study aims to determine the resistant phenotypes of these bacteria while describing their epidemiological aspect. Material and Methods: This was a cross-sectional study by consecutive enrollment from January 2018 to December 2019 at Sominé DOLO Hospital of Mopti, Mali. We performed manual method for bacteria culture, identification and antibiotics sensitivity testing. The antibiotics sensitivity testing was accessed by the diffusion method according to CA-SFM/EUCAST (“Comité de l’Antibiogramme de la Société Française de Microbiologie” / European Committee on Antimicrobial Susceptibility Testing) recommendations V1.0 february 2018 and V2.0 may 2019 2019 V.2.0. May recommendation released in 2019. Data were analyzed by software R 4.0.3 GUI 1.73 Catalina build (7892). Results: At all 904 samples were included in this study. Out of the 904 cultures, 297 sample (32.85%) were positive. The rates of enzymes production were as follow: Extended-spectrum ß-lactamase (ESBL) 56.42% (101/179), cepholosporinase hyperproduction (HCASE) 15.64% (28/179), cephalosporinase production (CASE) 6.14% (11/179), penicillinase hyperproduction (HP) 5.58% (10/179), carbapenemase production (CP) 6.14% (11/179) and savage strains 10.05% (18/179). Conclusion: Our data showed a high prevalence of resistance to β-lactamins ß-talactamins and carbapenemes in gram-negative bacteria and non-fermenting bacillus bacilli. The A high level of β-lactamase and carbapenemase production by gram negative bacillus were also reported by others authors calls for the rational use of antibiotic in hospital setting.

scholarly journals 660. Evaluation of Qvella’s FAST-Prep™ Liquid Colony™ for Early Antimicrobial Sensitivity Testing of Positive Blood Culture by Disk Diffusion Method

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S386-S386
Author(s):  
Susan M Novak-Weekley ◽  
Aye Aye Khine ◽  
Tino Alavie ◽  
Namidha Fernandez ◽  
Laxman Pandey ◽  
...  
Keyword(s):  
Direct Method ◽  
Viable Cell ◽  
Turnaround Time ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Sensitivity Testing ◽  
Gram Positive ◽  
Disk Diffusion Method ◽  
Gram Negative

Abstract Background Conventional antimicrobial susceptibility testing (AST) of microorganisms from positive blood cultures (PBC) can take ≥ 2 days. In order to improve the turnaround time for AST on a PBC, CLSI and EUCAST have made efforts to standardize procedures for disk diffusion (DD) direct from a PBC. Qvella Corporation (Richmond Hill, ON, Canada) has recently developed FAST-Prep, an automated centrifugal sample preparation system that rapidly delivers a Liquid Colony consisting of a purified, concentrated, viable cell suspension directly from a PBC. This study was performed to investigate the feasibility of DD AST off of a PBC using a FAST-Prep Liquid Colony. Methods Contrived PBC samples were prepared by spiking 6 species of Gram-positive and 4 species of Gram-negative bacteria (3-5 strains per species) into FA® Plus bottles and incubating in the BACT/ALERT® VIRTUO® System (bioMerieux, Durham, NC). After positivity, 3 mL of PBC was added to the FAST-Prep cartridge. After 20 minutes of processing in the FAST-Prep instrument, the Liquid Colony was removed from the cartridge and a 0.5 McFarland sample was prepared for DD AST. In parallel, the DD AST from a PBC was performed using 4 drops of PBC (CLSI direct method). Both methods were compared to conventional colony-based DD AST. After 16-18 hours of incubation zone diameters and S/I/R interpretations were determined. Categorical agreement (CA) and errors for both DD AST methods were calculated. In addition, colony plate counting was performed on 0.5 McFarland suspensions of Liquid Colony and the plate colony to determine biomass recovery and sample purity. Results CA for a FAST-Prep DD AST for Gram-positive and Gram-negative bacteria was 95.6% and 98.6%, respectively, compared to CA for CLSI DD AST of 77.2% and 81.9%, respectively. Biomass in the Liquid Colony was 7.2x108 and 1.2x109 CFU for Gram-positive and Gram-negative bacteria, respectively. Cell concentration in the 0.5 McFarland suspension of the Liquid Colony was 3.7x107 and 5.9x107 CFU/mL for Gram-positive and Gram-negative bacteria, respectively, which was similar to the concentration for the reference colony suspension. Conclusion The results support the potential role of FAST-Prep in providing a Liquid Colony for use in rapid AST. Disclosures Susan M. Novak-Weekley, PhD, D(ABMM), Qvella (Employee, Shareholder) Aye Aye Khine, PhD, Qvella (Employee, Shareholder) Tino Alavie, PhD, Qvella (Employee) Namidha Fernandez, MS, Qvella (Employee) Laxman Pandey, MS, Qvella (Employee) Abdossamad Talebpour, PhD, Qvella (Employee, Shareholder)

scholarly journals Cystic fibrosis pathogens survive for extended periods within cough-generated droplet nuclei

Thorax ◽  
2018 ◽  
Vol 74 (1) ◽  
pp. 87-90 ◽  
Author(s):  
Michelle E Wood ◽  
Rebecca E Stockwell ◽  
Graham R Johnson ◽  
Kay A Ramsay ◽  
Laura J Sherrard ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Cross Sectional Study ◽  
Gram Negative Bacteria ◽  
Sectional Study ◽  
Bacterial Strains ◽  
Cross Sectional ◽  
Gram Negative ◽  
Droplet Nuclei

The airborne route is a potential pathway in the person-to-person transmission of bacterial strains among cystic fibrosis (CF) populations. In this cross-sectional study, we investigate the physical properties and survival of common non-Pseudomonas aeruginosa CF pathogens generated during coughing. We conclude that Gram-negative bacteria and Staphylococcus aureus are aerosolised during coughing, can travel up to 4 m and remain viable within droplet nuclei for up to 45 min. These results suggest that airborne person-to-person transmission is plausible for the CF pathogens we measured.

scholarly journals Carbapenem-resistant Gram-negative bacteria isolated from poultry samples: a cross-sectional study

The Lancet ◽  
2019 ◽  
Vol 393 ◽  
pp. S21
Author(s):  
Abdelraouf A Elmanama ◽  
Mariam R Al-Reefi ◽  
Madleen A Shamali ◽  
Haya I Hemaid
Keyword(s):  
Cross Sectional Study ◽  
Gram Negative Bacteria ◽  
Sectional Study ◽  
Cross Sectional ◽  
Gram Negative ◽  
Carbapenem Resistant

scholarly journals Bad Bugs and No Drugs: Activity of Colistin as Waging War against Emerging Metallo-β-Lactamases Producing Pathogens

2018 ◽  
Vol 24 (1) ◽  
pp. 625-631 ◽  
Author(s):  
Sahar Naz ◽  
Farhan Rasheed ◽  
Muhammad Saeed ◽  
Shagufta Iram ◽  
Ambereen Anwar Imran
Keyword(s):  
Cross Sectional Study ◽  
Resistant Isolate ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Klebsiella Species ◽  
Cross Sectional ◽  
Acinetobacter Species ◽  
Gram Negative ◽  
Negative Results ◽  
Carbapenem Resistant

Inter-hospital and intra-hospital dissemination of metallo-β-lactamase (MβL) producing strains possess significant therapeutic challenges. Objective: This study was carried out to evaluate the efficacy of Colistin against MβL producers. Material and Methods: This cross-sectional study was conducted in Microbiology Laboratory, Allama Iqbal Medical College, Lahore, Pakistan from 1stJuly 2016 to 25th February 2017. A total of 12126 clinical samples were collected from patients presenting to Jinnah Hospital, Lahore. Every sample was processed for bacterial culture. Bacterial identification was performed according to standard guidelines. Every gram-negative isolate was further processed for antimicrobial susceptibility testing by modified Kirby Baur disc diffusion method. Zone sizes were interpreted according to CLSI 2016 guidelines. Next day every carbapenem-resistant isolate were further processed for MβL detection by EDTA method, zone size of Carbapenem disc only and Carbapenem disc impregnated with EDTA was compared ( >7 mm increase MβL positive, 0-5 mm increase MβL-negative). Results: Out of total 12126 samples, 35.9% (n=4361) were culture positive and only 40.5% (n=1770) were Gram negative rods. Of these 9.6% (n=170) were Carbapenem-resistant isolates with 47% (n=80) MβL producers. Briefly 51.7% (n=30) Acinetobacter species were MβL positive, Pseudomonas species 38.5% (n=22), Escherichia coli 69.5% (n=16), Klebsiella species 37.0% (n=10), Proteus 66.6% (n=2) and 0% Citrobacter sppwere MβL positive. 32.5% MβL positive isolates were from ICU, 21.2% were from OPD, 12.5%were from Surgical Units, 12.5% were from Medical Unit, 17.5% were from Orthopedic Unit, and 3.7% were from Pulmonology ward. Almost 100% resistant was observed in MβL positive isolates for Imipenem,Piperacillin+ Tazobactum, Ceftriaxone, Co-amoxyclav, Cefoperazone+Sulbactam, Ciprofloxacin, and Amikacin, Doxycycline, and Gentamicin showed 91.2%, 94.0%, and 97.5% resistant rate respectively. No resistance was observed against Colistin. Conclusion: MβL producing Gram negative rods are rising in clinical setups. They are becoming a nightmare for clinicians to treat such infections. Colistin remains the only choice of drug for MβL positive and Negative isolates with 0% resistant rate except for Proteus species, to which it is intrinsically resistant.

Association between level of care and colonization with resistant gram-negative bacteria among nursing-home residents

2021 ◽  
pp. 1-3
Author(s):  
Timileyin Y Adediran ◽  
Kinjal N Sethuraman ◽  
Surbhi Leekha ◽  
Mary-Claire Roghmann
Keyword(s):  
Nursing Home ◽  
Odds Ratio ◽  
Nursing Home Residents ◽  
Cross Sectional Study ◽  
Level Of Care ◽  
Gram Negative Bacteria ◽  
Sectional Study ◽  
Cross Sectional ◽  
Gram Negative ◽  
The Relationship

Abstract In this cross-sectional study, we examined the relationship between resident level of care in the nursing home and colonization with resistant gram-negative bacteria. Residential-care residents were more likely to be colonized with resistant gram-negative bacteria than were postacute care residents (odds ratio, 2.3; 95% confidence interval, 1.40–3.80; P < .001).

scholarly journals Gram Negative Bacteria Associated with Spoilage of Tomatoes Sold at Mararaba and Masaka Market, New Karu Local Government Area, Nasarawa State

2019 ◽  
pp. 1-7
Author(s):  
A. L. Olokun ◽  
A. B. Ajide ◽  
J. O. Ihuma
Keyword(s):  
Local Government ◽  
Pathogenic Bacteria ◽  
Cross Sectional Study ◽  
Local Government Area ◽  
Biochemical Test ◽  
Gram Negative Bacteria ◽  
Salmonella Spp ◽  
Cross Sectional ◽  
Gram Negative ◽  
Esherichia Coli

Aims: The study is aimed at isolating and identifying the gram negative bacteria associated with spoilage of tomatoes at Mararaba and Masaka markets, New Karu Local Government Area, Nasarawa State. Study Design: A cross-sectional study to isolate gram negative bacteria associated with spoilage of tomatoes at Mararaba and Masaka markets, New Karu Local Government Area, Nasarawa State. Place and Duration of Study: The isolation of gram negative bacteria was carried out at the Department of Biological Sciences, Bingham University New Karu, Nasarawa State, Nigeria, between January, 2018 and September, 2018. Methodology: The samples collected were crushed with a sterile mortar and pestle after which tenfold serial dilution was carried out using sterile distilled water before inoculating on MaCconkey and Blood agar using the pour plate technique. Colonies were then subjected to gram staining and biochemical test. Results: From the result of the biochemical test, Salmonella spp, Esherichia coli spp, Klebsiella spp and vibrio spp. were the gram-negative bacteria isolated from the tomato samples collected at both markets. Conclusion: Tomatoes sold in an unclean environment that is contamination with food- borne pathogenic bacteria poses a risk to the consumers.

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