scholarly journals Gram Negative Bacteria Associated with Spoilage of Tomatoes Sold at Mararaba and Masaka Market, New Karu Local Government Area, Nasarawa State

Author(s):  
A. L. Olokun ◽  
A. B. Ajide ◽  
J. O. Ihuma

Aims: The study is aimed at isolating and identifying the gram negative bacteria associated with spoilage of tomatoes at Mararaba and Masaka markets, New Karu Local Government Area, Nasarawa State. Study Design: A cross-sectional study to isolate gram negative bacteria associated with spoilage of tomatoes at Mararaba and Masaka markets, New Karu Local Government Area, Nasarawa State. Place and Duration of Study: The isolation of gram negative bacteria was carried out at the Department of Biological Sciences, Bingham University New Karu, Nasarawa State, Nigeria, between January, 2018 and September, 2018. Methodology: The samples collected were crushed with a sterile mortar and pestle after which tenfold serial dilution was carried out using sterile distilled water before inoculating on MaCconkey and Blood agar using the pour plate technique. Colonies were then subjected to gram staining and biochemical test. Results: From the result of the biochemical test, Salmonella spp, Esherichia coli spp, Klebsiella spp and vibrio spp. were the gram-negative bacteria isolated from the tomato samples collected at both markets. Conclusion: Tomatoes sold in an unclean environment that is contamination with food- borne pathogenic bacteria poses a risk to the consumers.

2020 ◽  
Vol 4 (6) ◽  
Author(s):  
A.B. Umar ◽  
A.H. Dankaka ◽  
M. Manjur Shah

The study is carried out to standardize larval indices namely House Index, Container Index and Breteau Index and also to identify the major breeding sources of mosquitoes in the residential environment in and around the Gwale Local government area of  Kano, Nigeria. A cross-sectional study by selecting 50 houses randomly from the 6 wards of Municipality was employed. Every water holding container indoors and outdoors were counted and searched for larval presence and noted on a pretested format. In this study, 300 houses were surveyed in 21 days out of which 94.33% (283 houses) were found to have potential sources for mosquito breeding. All the entomological indices were found to be above the critical level. House Index = 28.67%; Container Index = 12.14%; Breteau Index = 64.00% showing high chances for outbreaks of mosquito borne diseases. The area is prone to mosquito borne disease like malaria and therefore warrants interventions from the competent.


Thorax ◽  
2018 ◽  
Vol 74 (1) ◽  
pp. 87-90 ◽  
Author(s):  
Michelle E Wood ◽  
Rebecca E Stockwell ◽  
Graham R Johnson ◽  
Kay A Ramsay ◽  
Laura J Sherrard ◽  
...  

The airborne route is a potential pathway in the person-to-person transmission of bacterial strains among cystic fibrosis (CF) populations. In this cross-sectional study, we investigate the physical properties and survival of common non-Pseudomonas aeruginosa CF pathogens generated during coughing. We conclude that Gram-negative bacteria and Staphylococcus aureus are aerosolised during coughing, can travel up to 4 m and remain viable within droplet nuclei for up to 45 min. These results suggest that airborne person-to-person transmission is plausible for the CF pathogens we measured.


The Lancet ◽  
2019 ◽  
Vol 393 ◽  
pp. S21
Author(s):  
Abdelraouf A Elmanama ◽  
Mariam R Al-Reefi ◽  
Madleen A Shamali ◽  
Haya I Hemaid

Author(s):  
Shalangwa Ishaku Bata ◽  
Shalom Kumra Byencit

Background and Aim: Toxoplasma gondii is a parasite of public health significance due to its possible transmission to humans through ingestion of tissue cysts in raw or undercooked meat or food or water contaminated with oocysts shed by felids and transplacental transmission. This study was carried out to provide information on the possible risk of transmission through eating undercooked donkey meat by conducting a market based cross-sectional study design to determine the presence of T. gondii antibodies in trade donkeys in Ganawuri district, Riyom Local Government Area, Plateau State, North Central, Nigeria. Materials and Methods: One hundred and thirteen serum samples were collected from trade donkeys at the market and analyzed for T. gondii antibodies using latex agglutination test (LAT). Serum samples with LAT titer >10 μl/ml were considered positive. Results: The distribution of the donkeys based on their sources showed that greater proportions (61.1%) were from North Eastern part of the country. The study showed that 31 of the serum samples collected and analyzed were positive for T. gondii antibodies given an overall prevalence of 27.4%. The prevalence ranges between 22.2 and 33.3% across the states where the donkeys were sourced. The study showed that seropositivity increases with age (p=0.003; OR=11.8) and ranges between 12.5 and 47.2%. The prevalence did not vary significantly based on sex and source/location (p=0.494, OR=0.05; 0.920, 1.45, respectively). Conclusion: This study showed that trade donkeys at Ganawuri district market have antibodies to T. gondii and suggest a public health risk from the consumption of undercooked donkey meat.


Author(s):  
Timileyin Y Adediran ◽  
Kinjal N Sethuraman ◽  
Surbhi Leekha ◽  
Mary-Claire Roghmann

Abstract In this cross-sectional study, we examined the relationship between resident level of care in the nursing home and colonization with resistant gram-negative bacteria. Residential-care residents were more likely to be colonized with resistant gram-negative bacteria than were postacute care residents (odds ratio, 2.3; 95% confidence interval, 1.40–3.80; P < .001).


2022 ◽  
Vol 18 (1) ◽  
pp. 008-013
Author(s):  
Moussa Diawara ◽  
Modibo Coulibaly ◽  
Dramane Samaké ◽  
Soumaila Touré ◽  
Dramane Cissé ◽  
...  

Background: β-lactams and carbapenems. are the major antibiotics used to treat gram-negative bacteria and non-fermenting bacilli. However, the increasing production of β-lactamase and carbapenemase limits the therapeutic options. Our study aims to determine the resistant phenotypes of these bacteria while describing their epidemiological aspect. Material and Methods: This was a cross-sectional study by consecutive enrollment from January 2018 to December 2019 at Sominé DOLO Hospital of Mopti, Mali. We performed manual method for bacteria culture, identification and antibiotics sensitivity testing. The antibiotics sensitivity testing was accessed by the diffusion method according to CA-SFM/EUCAST (“Comité de l’Antibiogramme de la Société Française de Microbiologie” / European Committee on Antimicrobial Susceptibility Testing) recommendations V1.0 february 2018 and V2.0 may 2019 2019 V.2.0. May recommendation released in 2019. Data were analyzed by software R 4.0.3 GUI 1.73 Catalina build (7892). Results: At all 904 samples were included in this study. Out of the 904 cultures, 297 sample (32.85%) were positive. The rates of enzymes production were as follow: Extended-spectrum ß-lactamase (ESBL) 56.42% (101/179), cepholosporinase hyperproduction (HCASE) 15.64% (28/179), cephalosporinase production (CASE) 6.14% (11/179), penicillinase hyperproduction (HP) 5.58% (10/179), carbapenemase production (CP) 6.14% (11/179) and savage strains 10.05% (18/179). Conclusion: Our data showed a high prevalence of resistance to β-lactamins ß-talactamins and carbapenemes in gram-negative bacteria and non-fermenting bacillus bacilli. The A high level of β-lactamase and carbapenemase production by gram negative bacillus were also reported by others authors calls for the rational use of antibiotic in hospital setting.


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