scholarly journals Hard tissue inducibility of a novel synthetic peptide in rat pulp

2020 ◽  
Vol 8 (3) ◽  
pp. 392-401
Author(s):  
Nozomi Matsuo ◽  
Norimasa Tsuji ◽  
Kazuya Tominaga ◽  
Hiroshi Maeda
Keyword(s):  
Synthetic Peptide ◽  
Ct Images ◽  
Enamel Matrix Derivative ◽  
Control Group ◽  
Tissue Formation ◽  
Hard Tissue ◽  
Peptide Group ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Pulp Cavity

Enamel matrix derivative (EMD) is a periodontal tissue regeneration material that induced hard tissue formation. This study was performed to examine the reaction of dental pulp tissue directly capped with a synthetic peptide prepared based on EMD research, and to investigate its potential as a pulp capping material. The experiments were performed in the maxillary left and right first molars of five 6-week-old male Sprague–Dawley rats. The exposed pulp was capped with synthetic peptide in the peptide group, but left uncapped in the control group. Two weeks later, the maxillary bone was isolated and photographed by microfocus computed tomography (CT). The opaque area and volume of the coronal pulp cavity were measured on CT images, and the area-to-volume ratio was calculated. After decalcification, tissue samples were prepared, stained with hematoxylin and eosin (H&E), and examined histopathologically. CT images for both groups showed an opaque area in the coronal pulp cavity, and the fraction of images showing opacity was significantly higher in the peptide group (P < 0.01). A large amount of hard tissue was formed in the coronal pulp cavity in the peptide group, around which aggregation of cells with eosinophilic substances in polytopes was observed. Only slight hard tissue formation was observed in the control group. As it showed eosinophilicity on H&E staining, the synthetic peptide was suggested to be involved in hard tissue formation. Synthetic peptides can potentially be used as pulp capping materials.

scholarly journals Clinical and Histological Evaluation of Direct Pulp Capping on Human Pulp Tissue Using a Dentin Adhesive System

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Alicja Nowicka ◽  
Ryta Łagocka ◽  
Mariusz Lipski ◽  
Mirosław Parafiniuk ◽  
Katarzyna Grocholewicz ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Third Molar ◽  
Adhesive System ◽  
Histological Evaluation ◽  
Control Group ◽  
Single Bond ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Hydroxide Group ◽  
Dentin Adhesive

Objective. This study presents a clinical and histological evaluation of human pulp tissue responses after direct capping using a new dentin adhesive system. Methods. Twenty-eight caries-free third molar teeth scheduled for extraction were evaluated. The pulps of 22 teeth were mechanically exposed and randomly assigned to 1 of 2 groups: Single Bond Universal or calcium hydroxide. Another group of 6 teeth acted as the intact control group. The periapical response was assayed, and a clinical examination was performed. The teeth were extracted after 6 weeks, and a histological analysis was performed. The pulp status was assessed, and the thickness of the dentin bridge was measured and categorized using a histological scoring system. Results. The clinical phase was asymptomatic for Single Bond Universal patients. Patients in the calcium hydroxide group reported mild symptoms of pain, although the histological examination revealed that dentin bridges with or without limited pulpitis had begun forming in each tooth. The universal adhesive system exhibited nonsignificantly increased histological signs of pulpitis (P>0.05) and a significantly weaker thin mineralized tissue layer (P<0.001) compared with the calcium hydroxide group. Conclusion. The results suggest that Single Bond Universal is inappropriate for human pulp capping; however, further long-term studies are needed to determine the biocompatibility of this agent.

scholarly journals Direct pulp capping with novel nanostructural materials based on calcium silicate systems and hydroxyapatite

2016 ◽  
Vol 63 (4) ◽  
pp. 183-192 ◽  
Author(s):  
Marijana Popović Bajić ◽  
Violeta Petrović ◽  
Vanja Opačić Galić ◽  
Vesna Danilović ◽  
Vukoman Jokanović ◽  
...  
Keyword(s):  
Calcium Silicate ◽  
Healing Process ◽  
Glass Ionomer Cement ◽  
Control Group ◽  
Therapeutic Effects ◽  
Direct Pulp Capping ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Nanostructural Material ◽  
Experimental Group

Summary Introduction Direct pulp capping is an important therapeutic method that has goal to provide formation of dentin bridge and healing process of the pulp. The aim of this study was to investigate the effects of new nanostructural materials based on calcium silicate systems and hydroxyapatite on exposed dental pulp in Vietnamese pigs. Material and Methods The study was conducted on 30 teeth of two Vietnamese pigs (Sus scrofa verus). On buccal surfaces of incisors, canines and first premolars, class V cavities were prepared with a small round bur and pulp horn was exposed. In the first experimental group (10 teeth) the perforation was covered with new nanostructural material based on calcium silicate systems (CS). In the second experimental group, the perforation was covered with compound of calcium silicate systems and hydroxyapatite (HA-CS) (10 teeth). In the control group, exposed pulp was covered with Pro Root MTA® (Dentsply Tulsa Dental, Johnson City, TN, USA) (10 teeth). All cavities were restored with glass ionomer cement (GC Fuji VIII, GC Corporation, Tokyo, Japan). Observation period was 28 days. After sacrificing the animals, histological preparations were done to analyze the presence of dentin bridge, inflammatory reaction of the pulp, pulp tissue reorganization and the presence of bacteria. Results Dentin bridge was observed in all teeth (experimental and control groups). Inflammation of the pulp was mild to moderate in all groups. Neoangiogenesis and many odontoblast like cells responsible for dentin bridge formation were detected. Necrosis was not observed in any case, neither the presence of Gram-positive bacteria in the pulp. Conclusion Histological analysis indicated favorable therapeutic effects of new nanostructural materials based on calcium silicate systems and hydroxyapatite for direct pulp capping in teeth of Vietnamese pigs.

scholarly journals THE EFFECT OF CALCIUM HYDROXIDE-PROPOLIS ON THE NUMBER OF MACROFAG CELLS ON THE WISTAR RAT DENTAL WITH PULPA PERFORMANCE

2020 ◽  
Vol 10 (2) ◽  
pp. 58
Author(s):  
Setyabudi Setyabudi ◽  
Devi Eka Juniarti ◽  
Ira Widjiastuti ◽  
Maughfirah Shintya Fathori
Keyword(s):  
Calcium Hydroxide ◽  
Cellular Response ◽  
Wistar Rats ◽  
Inflammatory Cells ◽  
Wistar Rat ◽  
Control Group ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Macrophage Cells ◽  
Combined Application

Background: Cellular response of dental pulp to existing lesions, caused by infiltration of inflammatory cells that migrate from blood vessels to the site of injury. Macrophages are the cells that most appear when inflammation occurs. Treatment for maintaining pulp tissue vitality is called pulp capping. Calcium hydroxide as a pulp capping material has the ability to trigger the growth of dentin bridges or remineralization, but calcium hydroxide can induce migration and proliferation of inflammatory cells. Currently the field of dentistry is developing propolis as an alternative pulp capping material. That is because propolis has anti-inflammatory properties. Objective: To analyze the effect of the combination of calcium hydroxide-propolis on the number of macrophage cells in the teeth of wistar rats with pulp perforation. Method: This study used 30 samples of Wistar rats which were preparated until perforation and then divided into 3 groups. The first group continued with filling with cention, the second group continued with application of calcium hydroxide and filled with cention, and the third group continued with the application of combination of calcium hydroxide and propolis and then being filledwithcention. Macrophage cell is calculated histopathologically by using compound light microscope on the 3rd day with 400x magnification. Results: The Kruskal-Wallis test results showed significant differences in macrophages after the combination of calcium hydroxide and propolis (p = 0,000). The combined application of calcium hydroxide and propolis showed higher macrophages than the application of calcium hydroxide and the control group. Conclusion: Application of calcium hydroxide - propolis combination was proven increase the number of macrophage cells in Wistar Rats (Rattus Norvegicus) with pulp perforation

Visible Light-Induced Crosslinkable Gelatin for Direct Pulp Capping

2012 ◽  
Vol 529-530 ◽  
pp. 543-546
Author(s):  
Mariko Nakamura ◽  
Yasuhiro Yoshida ◽  
Yoshihiro Ito
Keyword(s):  
Soft Tissue ◽  
Visible Light ◽  
Dental Pulp ◽  
Dental Materials ◽  
Direct Pulp Capping ◽  
Hard Tissue ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Dental Pulp Tissue

Direct pulp capping involves the application of dental materials to the exposed pulp in an attempt to act as a barrier, protect the dental pulp complex and preserve its vitality. The materials for direct pulp capping should ideally adhere to dental pulp tissue as soft tissue and dentin as hard tissue with tight sealing. We therefore developed visible light-induced crosslinkable gelatin which is capable of adhesive to soft tissue and hard tissue.

scholarly journals Histological effects of enamel matrix derivative on exposed dental pulp

2015 ◽  
Vol 143 (7-8) ◽  
pp. 397-403 ◽  
Author(s):  
Marijana Popovic-Bajic ◽  
Vesna Danilovic ◽  
Branislav Prokic ◽  
Bogomir Prokic ◽  
Milica Manojlovic ◽  
...  
Keyword(s):  
Healing Process ◽  
Glass Ionomer Cement ◽  
Enamel Matrix Derivative ◽  
Tooth Pulp ◽  
Control Group ◽  
Direct Pulp Capping ◽  
Bridge Formation ◽  
Pulp Capping ◽  
Tissue Reorganization ◽  
And Control

Introduction. Direct pulp capping procedure is a therapeutic application of a drug on exposed tooth pulp in order to ensure the closure of the pulp chamber and to allow the healing process to take place. Objective. The aim of this study was to examine the histological effects of Emdogain? on exposed tooth pulp of a Vietnamese pig (Sus scrofa verus). Methods. The study comprised 20 teeth of a Vietnamese pig. After class V preparation on the buccal surfaces of incisors, canines and first premolars, pulp was exposed. In the experimental group, the perforations were capped with Emdogain? (Straumann, Basel, Switzerland), while in the control group pulp capping was performed with MTA? (Dentsply Tulsa Dental, Johnson City, TN, USA). All cavities were restored with glass-ionomer cement (GC Fuji VIII, GC Corporation, Tokyo, Japan). The observational period was 28 days, after which the animal was sacrificed and histological preparations were made. A light microscope was used to analyze dentin bridge formation, tissue reorganization and inflammation, and the presence of bacteria in the pulp. Results. The formation of dentin bridge was observed in the experimental and control groups. Inflammation of the pulp was mild to moderate in both groups. Angiogenesis and many odontoblast-like cells, responsible for dentin bridge formation, were observed. Necrosis was not observed in any case, nor were bacteria present in the pulp. Conclusion. Histological analysis indicated a favorable therapeutic effect of Emdogain? Gel in direct pulp capping of Vietnamese pigs. Pulp reaction was similar to that of MTA?.

scholarly journals Hard tissue formation after direct pulp capping with osteostatin and MTA in vivo

2021 ◽  
Vol 46 ◽  
Author(s):  
Ji-Hye Yoon ◽  
Sung-Hyeon Choi ◽  
Jeong-Tae Koh ◽  
Bin-Na Lee ◽  
Hoon-Sang Chang ◽  
...  
Keyword(s):  
Tissue Formation ◽  
Direct Pulp Capping ◽  
Hard Tissue ◽  
Pulp Capping

scholarly journals Theracal lc: A boon to dentistry

2021 ◽  
Vol 11 (2) ◽  
pp. 112-117
Author(s):  
Kalyani G Umale ◽  
Vandana J Gade ◽  
Reema N Asani ◽  
Priya R. Kosare ◽  
Snehal Gaware ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Calcium Silicate ◽  
Tissue Formation ◽  
Hard Tissue ◽  
Setting Reaction ◽  
Pulp Capping ◽  
Composition Method ◽  
Calcium Silicates ◽  
Pulp Vitality ◽  
Postoperative Sensitivity

TheraCal LC, the focus of this article, is a material that creates a new category of resin-modified calcium silicates (RMCS). It is a light-cured, resin-based, and highly radiopaque liner designed to release calcium to promote hard-tissue formation, and is indicated for use under direct restorative materials as a replacement to calcium hydroxide and other calcium silicate-based materials, glass ionomers, eugenol-based sedative materials, and pulp capping restoratives. TheraCal LC exhibits several properties to help maintain ideal hard-tissue health and to reduce the incidence of postoperative sensitivity. This article is aimed to review the composition, method of application, setting reaction, properties and uses of TheraCal LC. TheraCal LC is interesting and promising product, which have the potential of creating major contributions to maintaining pulp vitality.

scholarly journals Hard tissue formation induced by synthetic oligopeptide derived from an enamel matrix derivative

2012 ◽  
Vol 16 (3/4) ◽  
pp. 75-80 ◽  
Author(s):  
Michiko Noguchi ◽  
Kazuya Tominaga ◽  
Akio Tanaka ◽  
Masatoshi Ueda
Keyword(s):  
Enamel Matrix Derivative ◽  
Tissue Formation ◽  
Enamel Matrix ◽  
Hard Tissue ◽  
Matrix Derivative

scholarly journals Pulpal Response to the Combined Use of Mineral Trioxide Aggregate and Iloprost for Direct Pulp Capping

2021 ◽  
Vol 11 (8) ◽  
pp. 3702
Author(s):  
AlAnoud Almeshari ◽  
Rita Khounganian ◽  
Wael Mahdi ◽  
Fahd Aljarbou ◽  
Shilpa Bhandi ◽  
...  
Keyword(s):  
Mineral Trioxide Aggregate ◽  
Control Group ◽  
Dentinal Tubule ◽  
Glass Ionomer ◽  
Hard Tissue ◽  
Inflammatory Reactions ◽  
Pulp Capping ◽  
Tissue Degeneration ◽  
Clinically Significant ◽  
Dunnett's Test

Purpose: The present study aims to assess the combined effects of mineral trioxide aggregate (MTA) and iloprost when used as a pulp capping material on pulpal inflammation and tertiary dentin formation compared with MTA and iloprost alone in rat molar teeth. Methods: Eighty maxillary first molar rat teeth were exposed and capped with iloprost solution, MTA, or MTA mixed with iloprost (MTA-iloprost). The cavities were then filled with resin-modified glass ionomer. The cavity was restored with glass ionomer without the use of pulp capping agent in the control group. The rats were sacrificed after one and four weeks. Block sections of the molar specimens were prepared and subjected to hematoxylin and eosin staining for evaluation. Statistical analysis was done using the Kruskal–Wallis test, followed by Dunnett’s test. Results: At week one, the control group showed significantly more severe pulpal inflammatory reactions than the iloprost (p = 0.00), MTA (p = 0.04), and MTA-iloprost (p = 0.00) groups. Hard tissue formation was commonly found in the iloprost, MTA, and MTA-iloprost groups. After four weeks, pulpal tissue degeneration was observed in the control group. Complete hard tissue barriers were found in 50%, 72.7%, and 77.8% of the specimens in iloprost, MTA, and MTA-iloprost groups, respectively, with no significant differences among the experimental groups. The dentinal tubule patterns were mostly regular in the MTA-iloprost group and irregular in the iloprost and MTA groups. Conclusions: The application of iloprost, MTA, and MTA-iloprost as a pulp capping material resulted in similar pulpal responses in the mechanically exposed pulp of rat molars. Therefore, mixing MTA with iloprost might not be clinically significant.

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