Visible Light-Induced Crosslinkable Gelatin for Direct Pulp Capping

2012 ◽  
Vol 529-530 ◽  
pp. 543-546
Author(s):  
Mariko Nakamura ◽  
Yasuhiro Yoshida ◽  
Yoshihiro Ito
Keyword(s):  
Soft Tissue ◽  
Visible Light ◽  
Dental Pulp ◽  
Dental Materials ◽  
Direct Pulp Capping ◽  
Hard Tissue ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Dental Pulp Tissue

Direct pulp capping involves the application of dental materials to the exposed pulp in an attempt to act as a barrier, protect the dental pulp complex and preserve its vitality. The materials for direct pulp capping should ideally adhere to dental pulp tissue as soft tissue and dentin as hard tissue with tight sealing. We therefore developed visible light-induced crosslinkable gelatin which is capable of adhesive to soft tissue and hard tissue.

Analysis of hard tissue regeneration and Wnt signalling in dental pulp tissues after direct pulp capping with different materials

2019 ◽  
Vol 52 (11) ◽  
pp. 1605-1616 ◽  
Author(s):  
K. Yaemkleebbua ◽  
T. Osathanon ◽  
N. Nowwarote ◽  
C. N. Limjeerajarus ◽  
W. Sukarawan
Keyword(s):  
Tissue Regeneration ◽  
Dental Pulp ◽  
Wnt Signalling ◽  
Direct Pulp Capping ◽  
Hard Tissue ◽  
Pulp Capping

scholarly journals Dexamethasone - loaded polymeric porous sponge as a hard tissue regeneration agent

2020 ◽  
Author(s):  
Amjad Alagha
Keyword(s):  
Stem Cells ◽  
Inflammatory Response ◽  
Tissue Regeneration ◽  
Animal Experiment ◽  
Direct Pulp Capping ◽  
Hard Tissue ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Odontoblast Differentiation

Abstract Background : The aim of this study is to achieve the principles of tissue engineering using biopolymers to be applied in the field of vital endodontic treatment with the aim of stimulating stem cells and engineering and regeneration of dentin tissue. the blend was loaded with the steroidal anti-inflammatory drug, dexamethasone, and the porous drug-loaded bio-sponge was produced by lyophilization. Bio-sponge, as a direct pulp capping agent, was histologically studied compared to calcium hydroxide Ca(OH)2 in an animal experiment. Results: The results indicated the effectiveness of the bio-sponge as a direct pulp agent, where the dentin bridge was formed faster than Ca(OH)2 treated samples,. There was no inflammatory response in the pulp tissue throughout the follow-up period. Conclusions : The porous bio-sponge loaded with dexamethasone with a neutral pH resulted in enhancement the odontoblast differentiation from stem cells, resulted in the formation of a renewed dentin bridge without the slightest inflammatory response in the pulp.

scholarly journals Odontoblast-Like Cells Differentiated from Dental Pulp Stem Cells Retain Their Phenotype after Subcultivation

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Paula A. Baldión ◽  
Myriam L. Velandia-Romero ◽  
Jaime E. Castellanos
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Matrix Protein ◽  
Dental Materials ◽  
Dental Pulp Stem Cells ◽  
Cell Physiology ◽  
Pulp Tissue ◽  
Alizarin Red ◽  
Dentin Matrix Protein ◽  
Mineral Deposition

Odontoblasts, the main cell type in teeth pulp tissue, are not cultivable and they are responsible for the first line of response after dental restauration. Studies on dental materials cytotoxicity and odontoblast cells physiology require large quantity of homogenous cells retaining most of the phenotype characteristics. Odontoblast-like cells (OLC) were differentiated from human dental pulp stem cells using differentiation medium (containing TGF-β1), and OLC expanded after trypsinization (EXP-21) were evaluated and compared. Despite a slower cell growth curve, EXP-21 cells express similarly the odontoblast markers dentinal sialophosphoprotein and dentin matrix protein-1 concomitantly with RUNX2 transcripts and low alkaline phosphatase activity as expected. Both OLC and EXP-21 cells showed similar mineral deposition activity evidenced by alizarin red and von Kossa staining. These results pointed out minor changes in phenotype of subcultured EXP-21 regarding the primarily differentiated OLC, making the subcultivation of these cells a useful strategy to obtain odontoblasts for biocompatibility or cell physiology studies in dentistry.

scholarly journals Dental Pulp Tissue Regeneration Using Dental Pulp Stem Cells Isolated and Expanded in Human Serum

2017 ◽  
Vol 43 (4) ◽  
pp. 568-574 ◽  
Author(s):  
Evandro Piva ◽  
Susan A. Tarlé ◽  
Jacques E. Nör ◽  
Duohong Zou ◽  
Elizabeth Hatfield ◽  
...  
Keyword(s):  
Stem Cells ◽  
Human Serum ◽  
Tissue Regeneration ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Pulp Tissue ◽  
Dental Pulp Tissue ◽  
Pulp Tissue Regeneration

scholarly journals Direct pulp capping with novel nanostructural materials based on calcium silicate systems and hydroxyapatite

2016 ◽  
Vol 63 (4) ◽  
pp. 183-192 ◽  
Author(s):  
Marijana Popović Bajić ◽  
Violeta Petrović ◽  
Vanja Opačić Galić ◽  
Vesna Danilović ◽  
Vukoman Jokanović ◽  
...  
Keyword(s):  
Calcium Silicate ◽  
Healing Process ◽  
Glass Ionomer Cement ◽  
Control Group ◽  
Therapeutic Effects ◽  
Direct Pulp Capping ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Nanostructural Material ◽  
Experimental Group

Summary Introduction Direct pulp capping is an important therapeutic method that has goal to provide formation of dentin bridge and healing process of the pulp. The aim of this study was to investigate the effects of new nanostructural materials based on calcium silicate systems and hydroxyapatite on exposed dental pulp in Vietnamese pigs. Material and Methods The study was conducted on 30 teeth of two Vietnamese pigs (Sus scrofa verus). On buccal surfaces of incisors, canines and first premolars, class V cavities were prepared with a small round bur and pulp horn was exposed. In the first experimental group (10 teeth) the perforation was covered with new nanostructural material based on calcium silicate systems (CS). In the second experimental group, the perforation was covered with compound of calcium silicate systems and hydroxyapatite (HA-CS) (10 teeth). In the control group, exposed pulp was covered with Pro Root MTA® (Dentsply Tulsa Dental, Johnson City, TN, USA) (10 teeth). All cavities were restored with glass ionomer cement (GC Fuji VIII, GC Corporation, Tokyo, Japan). Observation period was 28 days. After sacrificing the animals, histological preparations were done to analyze the presence of dentin bridge, inflammatory reaction of the pulp, pulp tissue reorganization and the presence of bacteria. Results Dentin bridge was observed in all teeth (experimental and control groups). Inflammation of the pulp was mild to moderate in all groups. Neoangiogenesis and many odontoblast like cells responsible for dentin bridge formation were detected. Necrosis was not observed in any case, neither the presence of Gram-positive bacteria in the pulp. Conclusion Histological analysis indicated favorable therapeutic effects of new nanostructural materials based on calcium silicate systems and hydroxyapatite for direct pulp capping in teeth of Vietnamese pigs.

scholarly journals Scaff olds for dental pulp tissue regeneration: A review

2016 ◽  
Vol 2 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Saaid Ayesh Alshehadat ◽  
Htun Aung Thu ◽  
Suzina Sheikh Abdul Hamid ◽  
Asma Abdullah Nurul ◽  
Samsudin Abdul Rani ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Dental Pulp ◽  
Pulp Tissue ◽  
Dental Pulp Tissue ◽  
Pulp Tissue Regeneration
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