Performance and Consistency of Circulating Warm Water Blankets for Rodents

2022 ◽  
Author(s):  
Imani N Nicolis ◽  
Corinna N Beale ◽  
Willie A Bidot ◽  
Michael Esmail ◽  
Scott E Perkins
Keyword(s):  
Quality Control ◽  
Adverse Effects ◽  
General Anesthesia ◽  
Heat Pump ◽  
Respiratory Function ◽  
External Heat ◽  
Control Measures ◽  
Warm Water ◽  
Thermoneutral Zone ◽  
Preparation Phase

General anesthesia as used for rodent research can have adverse effects on physiologic mechanisms. Thermoregulation is often greatly inhibited, with resultant deleterious effects on cardiac and respiratory function. These potential effects can be mitigated by providing external heat support. The circulating warm water blanket and associated heat pump are often used in rodent procedures. The current study demonstrated that the heating pump and water blanket require quality control assessment to ensure adequate function. Our data showed that of the 6 pumps tested, 5 were able to achieve a temperature thatmet or exceeded the documented thermoneutral zone for mice. Pumps required 20 min of warming to reach their maximal attainable temperatures for the designated user setting. Although the pumps reached a temperature that was sufficient toprovide external thermal support, only 1 of the 6 pumps reached the temperature that was set by the user during the trial.Surface temperatures across the water blanket were recorded to analyze whether a difference in heat support was influencedby animal placement along the water blanket; however, the location points did not yield statistically different results. Two pumps were eliminated from the study due to failure to pass the preparation phase of the trial. The results of this studysupport the need for facilities to establish quality control measures to ensure that heat support systems are functioning at a level required to maintain normothermia during anesthetic procedures.

scholarly journals Effect of Preoperative Intravenous Oxycodone After Transurethral Resection of Prostate Under General Anesthesia

2017 ◽  
Vol 102 (7-8) ◽  
pp. 377-381
Author(s):  
Jinguo Wang ◽  
Yaowen Fu ◽  
Haichun Ma ◽  
Na Wang
Keyword(s):  
Urinary Tract ◽  
Postoperative Pain ◽  
Adverse Effects ◽  
General Anesthesia ◽  
Transurethral Resection ◽  
Lower Urinary Tract ◽  
Airway Device ◽  
Time Interval ◽  
Transurethral Resection Of Prostate ◽  
Lower Urinary

The objective of this study was to investigate the effect of intravenous oxycodone administered before transurethral resection of prostate (TURP) on postoperative pain and tramadol consumption. Preemptive analgesia can decrease postoperative pain and analgesic consumption. Sixty patients undergoing elective TURP were divided into 2 groups: group O (n = 30) received intravenous oxycodone (0.1 mg/kg) 10 minutes before surgery over 2 minutes and group C (n = 30) received normal saline as a placebo. A standardized general anesthesia method was performed with a laryngeal mask airway device. Data with respect to pain intensity, incidence of lower urinary tract discomfort, time to the first tramadol requirement, tramadol consumption, overall patient satisfaction, and adverse effects were collected. Preoperative oxycodone contributed to better analgesia at 1, 2, 6, and 12 hours after shifting the patients to the recovery room, a longer time interval to the first tramadol request, fewer patients requiring tramadol analgesia, and reduced tramadol consumption. Adverse effects were comparable between the 2 groups. Preoperative intravenous oxycodone (0.1 mg/kg) 10 minutes before TURP improves postoperative analgesia, prolongs time to the first tramadol request, and reduces tramadol consumption without an influence on lower urinary tract discomfort and adverse effects.

Microbiological Water Methods: Quality Control Measures for Federal Clean Water Act and Safe Drinking Water Act Regulatory Compliance

2014 ◽  
Vol 97 (2) ◽  
pp. 567-572 ◽  
Author(s):  
Patsy Root ◽  
Margo Hunt ◽  
Karla Fjeld ◽  
Laurie Kundrat
Keyword(s):  
Quality Control ◽  
Drinking Water ◽  
Clean Water Act ◽  
Regulatory Compliance ◽  
Control Measures ◽  
Clean Water ◽  
Safe Drinking Water ◽  
Safe Drinking Water Act ◽  
Specificity And Sensitivity ◽  
Microbiological Methods

Abstract Quality assurance (QA) and quality control (QC) data are required in order to have confidence in the results from analytical tests and the equipment used to produce those results. Some AOAC water methods include specific QA/QC procedures, frequencies, and acceptance criteria, but these are considered to be the minimum controls needed to perform a microbiological method successfully. Some regulatory programs, such as those at Code of Federal Regulations (CFR), Title 40, Part 136.7 for chemistry methods, require additional QA/QC measures beyond those listed in the method, which can also apply to microbiological methods. Essential QA/QC measures include sterility checks, reagent specificity and sensitivity checks, assessment of each analyst's capabilities, analysis of blind check samples, and evaluation of the presence of laboratory contamination and instrument calibration and checks. The details of these procedures, their performance frequency, and expected results are set out in this report as they apply to microbiological methods. The specific regulatory requirements of CFR Title 40 Part 136.7 for the Clean Water Act, the laboratory certification requirements of CFR Title 40 Part 141 for the Safe Drinking Water Act, and the International Organization for Standardization 17025 accreditation requirements under The NELAC Institute are also discussed.

scholarly journals Standardized quality control workflow to evaluate the reproducibility and differentiation potential of human iPSCs into neurons

2021 ◽  
Author(s):  
Carol X.-Q. Chen ◽  
Narges Abdian ◽  
Gilles Maussion ◽  
Rhalena A. Thomas ◽  
Iveta Demirova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Quality Control ◽  
Cortical Neurons ◽  
Genomic Stability ◽  
Control Measures ◽  
List Type ◽  
Ipsc Line ◽  
Differentiation Potential ◽  
Germ Layers ◽  
Human Ipscs

AbstractInduced pluripotent stem cells (iPSCs) derived from human somatic cells have created new opportunities to generate disease-relevant cells. Thus, as the use of patient-derived stem cells has become more widespread, having a workflow to monitor each line is critical. This ensures iPSCs pass a suite of quality control measures, promoting reproducibility across experiments and between labs. With this in mind, we established a four-step workflow to assess our newly generated iPSCs for variations and reproducibility relative to each other and iPSCs obtained from external sources. Our benchmarks for evaluating iPSCs include examining iPSC morphology and proliferation in two different media conditions (mTeSR1 and Essential 8) and evaluating their ability to differentiate into each of the three germ layers, with a particular focus on neurons. Genomic stability in the human iPSCs was analyzed by G-band karyotyping and a qPCR-based stability test, and cell-line identity authenticated by Short Tandem Repeat (STR) analysis. Using standardized dual SMAD inhibition methods, all iPSC lines gave rise to neural progenitors that could subsequently be differentiated into cortical neurons. Neural differentiation was analyzed qualitatively by immunocytochemistry and quantitatively by q-PCR for progenitor, neuronal, cortical and glial markers. Taken together, we present a standardized quality control workflow to evaluate variability and reproducibility across and between iPSCs.HighlightsValidation of culture conditions is critical in the expansion and maintenance of an iPSC line.Characterization of pluripotency and genomic stability ensures each line is free of defects at the DNA level, while maintaining its ability to be directed into any of the three germ layers.Forebrain cortical neurons can be generated from all iPSC line tested; however, the morphology and expression pattern of these neurons can vary from line to line.

scholarly journals Successful resuscitation of the patient on extended tocolytic therapy subjected to an urgent cesarean section

2002 ◽  
Vol 59 (3) ◽  
pp. 325-328 ◽  
Author(s):  
Biljana Djordjevic ◽  
Milos Stojiljkovic ◽  
Tatjana Potpara ◽  
Dragana Loncar-Stojiljkovic ◽  
Ljiljana Vojvodic
Keyword(s):  
Cardiac Arrest ◽  
Myocardial Ischemia ◽  
Adverse Effects ◽  
Cesarean Section ◽  
Maintenance Therapy ◽  
General Anesthesia ◽  
Preterm Labor ◽  
Intravenous Therapy ◽  
Successful Resuscitation ◽  
The Usa

Ritodrine is the only medicament approved by FDA in the USA as well as in our country for prevention of the threatening preterm labor. Its adverse effects upon the respiratory and cardiovascular systems, including pulmonary edema and myocardial ischemia, occur more frequently during the intravenous therapy than during the oral maintenance therapy. The aim of this report was to present a patient with cardiovascular adverse effects of ritodrine, who had her pregnancy terminated by an urgent cesarean section under general anesthesia. In the course of operation, the patient had two cardiac arrest (total of 70 min). Resuscitation was performed by direct and indirect heart massage. The patient's condition was stabilized during the next six hours. The patient was transferred to the coronary unit, where the treatment was continued for 30-days period, after which the patient was released home as completely recovered.

scholarly journals Oral Health Care Delivery for Children During COVID-19 Pandemic—A Retrospective Study

2021 ◽  
Vol 9 ◽  
Author(s):  
Avia Fux-Noy ◽  
Luna Mattar ◽  
Aviv Shmueli ◽  
Elinor Halperson ◽  
Diana Ram ◽  
...  
Keyword(s):  
General Anesthesia ◽  
Behavior Management ◽  
Deep Sedation ◽  
Care Delivery ◽  
Medical Center ◽  
Pediatric Dentistry ◽  
Control Measures ◽  
Healthy Children ◽  
Efficient Treatment ◽  
Dental Staff

Aim: COVID-19 outbreak and the lockdown period following was a very challenging time for pediatric dentistry. We aimed to find whether the characteristics of dental care provided to children at the Department of Pediatric Dentistry at Hadassah medical center, Jerusalem, Israel, differed between the periods, before COVID-19 outbreak, during the lockdown period and during the period that followed it.Materials and Methods: We retrospectively reviewed computerized records of patients who visited the pediatric dental clinic at three different periods: pre-lockdown period, lockdown period, and post-lockdown period.Results: Nine-hundred and forty-nine children were included in the study; most of them were healthy children between 3 and 6 years old. During lockdown, all scheduled appointments except for treatments under general anesthesia and deep sedation were canceled due to the government's restrictions; the frequency of treatments with non-pharmacological behavior management, general anesthesia or deep sedation was higher than in the previous or subsequent periods and the use of inhaled/conscious sedation was significantly lower. During lockdown most of the children were diagnosed with dentoalveolar abscess (32.3%), compared to 14 and 21% at the previous or subsequent periods, respectively (P < 0.001). Treatments combination during lockdown included more extractions, pulpectomies and pulp extirpation and less permanent restorations (P < 0.001). None of the staff members was infected with COVID-19 at the clinic during these periods. We concluded that dentists should be updated about Covid-19 modes of transmission and the recommended infection control measures in dental settings. Effective management protocols can help the dental staff to continue to provide efficient treatment and prevent Covid-19 contamination.

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