scholarly journals Isolation of Sulfur Oxidizing Bacteria from Polluted Water and Screening for Their Efficiency of Sulfide Oxidase Production

2018 ◽  
Vol 21 (3) ◽  
pp. 259-264
Keyword(s):  
Biochemical Characterization ◽  
Critical Role ◽  
Sulfur Cycle ◽  
Polluted Water ◽  
Bacterial Isolates ◽  
Sulfate Ion ◽  
Promising Alternative ◽  
The Media ◽  
Sulfur Oxidizing ◽  
Sulfur Oxidizing Bacteria

<p>Presence of sulfide in the environment represents huge concerns to biological life. The high costs and low safety of chemical and physical removal strategies lead to finding alternative strategies. Sulfur-oxidizing bacteria (SOB) are consider a promising alternative strategy, where they play a critical role in removal of such compounds from water and soil environments, as well as, they maintain the sulfur balance during natural sulfur cycle. The main objective of this study was to isolate sulfur oxidizing bacteria (SOB) from sulfide polluted water. In addition screening for sulfide oxidase producer bacteria was performed. Twenty isolates of sulfur oxidizing bacteria were isolated from eight different sulfide polluted water sources. During testing the isolates on thiosulfate broth medium, it was observed that, eleven isolates could efficiently reduce the pH of the media from 7.5±0.2 to 5.0 ± 0.5 as a resulting of the oxidizing of sulfides to sulfate ion. The concentration of produced sulfate ion ranged from 76 to 155 mg mL<sup>-1</sup>. The sulfur oxidase activity of the tested isolates ranged between 2.68 to 5.23 U mL<sup>-1</sup>. The bacterial isolates were identified as <em>Bacillus</em> spp., <em>Pseudomonas</em> spp. and <em>Klebsiella </em>spp. based on their morphological and biochemical characterization.</p>

scholarly journals Characterizations of sulfur oxidizing bacteria from extensive shrimp ponds

2021 ◽  
Vol 13 (Aquaculture) ◽  
pp. 86-95
Author(s):  
Truong Giang Huynh ◽  
Hung Hai Vu ◽  
Thi Cam Tu Phan ◽  
Thi Tuyet Ngan Pham ◽  
Ngoc Ut Vu
Keyword(s):  
Oxidase Activity ◽  
Water Quality Management ◽  
Sodium Thiosulfate ◽  
Sulfate Ion ◽  
Biochemical Tests ◽  
Shrimp Ponds ◽  
Ion Production ◽  
Oxidase Enzyme ◽  
Sulfur Oxidizing ◽  
Sulfur Oxidizing Bacteria

The aim of this study is to characterize the sulfur oxidizing bacteria (SOB) isolates from the sediments of extensive shrimp ponds for recommending the use of this group for water quality management in aquaculture. Sediment samples were collected from 12 extensive shrimp ponds located in Tra Vinh, Soc Trang, Bac Lieu, and Ca Mau provinces. To screen the potential sulfur oxidizing bacteria, medium was amended with sodium thiosulfate, and the sulfate ion production ability and sulfur oxidase enzyme activity of the isolates were measured spectrophotometrically. Results showed that 30 isolates grew on the thiosulfate agar medium. Among these, only five isolates reduced the pH of the growth medium and showed high sulfur oxidase activity and production of sulfate ion when isolates were inoculated with thiosulfate as a substrate. Physiological and biochemical tests indicated that five selected isolates were Gram negative, short rod, non-motile, non-spore forming, negative for oxidase reaction, and positive for catalase reaction. The isolates SOBTB1.1 and SOBTB6.2 showed the significantly higher sulfur oxidase activity and production of sulfate ion compared to other isolates. SOBTB6.2 isolate produced sulfate ion and exhibited higher sulfur oxidase activity at pH4-5, followed by pH6-7. It is, therefore, suggested that the SOBTB 1.1 and SOBTB6.2 could be promising sulfur oxidizers for further research and uses in aquaculture.

scholarly journals Effect of Sulfur-Oxidizing Bacteria Thiobacillus Thioparus and Different Levels of Agricultural Sulfur on Wheat Yield (Triticum Aestivium L.)

2021 ◽  
Vol 923 (1) ◽  
pp. 012074
Author(s):  
Abdullah Karim Jabbar ◽  
Dalia Qassem Al-Ziyadi
Keyword(s):  
Wheat Yield ◽  
Wheat Plant ◽  
Triticum Aestivum L ◽  
Bacterial Isolates ◽  
Agricultural Field ◽  
Thiobacillus Thioparus ◽  
Sulfur Oxidizing ◽  
Four Levels ◽  
Different Levels ◽  
Sulfur Oxidizing Bacteria

Abstract A field experiment was carried out in the agricultural season 2020-2021 in an agricultural field located in the Al-Jarboua area (Al Hafez) in Al-Muthanna Governorate to study the effect of inoculation with sulfur-oxidizing bacteria and agricultural sulfur levels and their interactions on the yield of wheat plant Triticum aestivum L. Ibaa cultivar 99. The study was carried out in two stages, the first is isolation Sulfur oxidizing bacteria and the second used as a inoculant in the agricultural experiment to study its effect on soil characteristics and its content of nutrients NPK and S. At two levels T0 without adding the bacterial inoculum and T1 adding the first isolate of bacteria The second factor included the addition of agricultural sulfur with four levels of sulfur (S0 were taken without addition, S1 added 750 kg ha−1, S2 added 1500 kg ha−1 and S3 added 2250 kg ha−1) In three replications, the number of experimental units reached 36 experimental units. The results of the study also showed that inoculation with bacterial isolates led to a significant increase in the readiness of nutrients N, P, K and S, and the highest rate of sulfur was (1895) mg. The pollination also led to a significant increase in the growth characteristics of the plant (plant height, number of spikes and grain yield), as it recorded (101.25 cm, 393.22 spike m−1 and 6.73 mcg/ha−1), respectively. The addition of agricultural sulfur at different levels affected a significant increase in the availability of nutrients N, P, K and S, and the highest rate of sulfur at the level of S3 was (1817) mg. Also, the bilateral interaction between inoculation with bacterial isolates and agricultural sulfur led to a significant increase in the availability of nutrients N, P, K and S. kg −1 soil.

Effects of activated carbon on treatment of photo-processing waste by sulfur-oxidizing bacteria

1997 ◽  
Vol 35 (7) ◽  
pp. 187-195 ◽  
Author(s):  
Binle Lin ◽  
K. Futono ◽  
A. Yokoi ◽  
M. Hosomi ◽  
A. Murakami
Keyword(s):  
Activated Carbon ◽  
Treatment Effectiveness ◽  
Environmental Concern ◽  
Type Systems ◽  
Continuous Treatment ◽  
Processing Waste ◽  
Treatment Technology ◽  
Adsorption Effect ◽  
Sulfur Oxidizing ◽  
Sulfur Oxidizing Bacteria

Establishing economic treatment technology for safe disposal of photo-processing waste (PW) has most recently become an urgent environmental concern. This paper describes a new biological treatment process for PW using sulfur-oxidizing bacteria (SOB) in conjunction with activated carbon (AC). Batch-type acclimation and adsorption experiments using SOB/PAC, SOB/PNAC, and SOB reactor type systems demonstrated that AC effectively adsorbs the toxic/refractory compounds which inhibit thiosulfate oxidization of SOB in PW. Thus, to further clarify the effect of AC, we performed a long-term (≈ 160 d) continuous-treatment experiment on 4- to 8-times dilution of PW using a SOB/GAC system which simulated a typical wastewater treatment system based on an aerobic activated sludge process that primarily uses acclimated SOB. The thiosulfate load and hydraulic retention time (HRT) were fixed during treatment such that they ranged from 0.8-3.7 kg S2O32-/l/d and 7.7-1.9 d, respectively. As expected, continuous treatment led to breakthrough of the adsorption effect of GAC. Renewing the GAC and continuing treatment for about 10 d demonstrated good treatment effectiveness.

scholarly journals Sulfur bacteria promote dissolution of authigenic carbonates at marine methane seeps

2021 ◽  
Author(s):  
Dalton J. Leprich ◽  
Beverly E. Flood ◽  
Peter R. Schroedl ◽  
Elizabeth Ricci ◽  
Jeffery J. Marlow ◽  
...  
Keyword(s):  
Carbonate Rocks ◽  
Sulfur Oxidation ◽  
Methane Seeps ◽  
Etch Pits ◽  
Active Dissolution ◽  
Authigenic Carbonates ◽  
Sulfur Bacteria ◽  
Sulfur Oxidizing ◽  
Sulfur Oxidizing Bacteria

AbstractCarbonate rocks at marine methane seeps are commonly colonized by sulfur-oxidizing bacteria that co-occur with etch pits that suggest active dissolution. We show that sulfur-oxidizing bacteria are abundant on the surface of an exemplar seep carbonate collected from Del Mar East Methane Seep Field, USA. We then used bioreactors containing aragonite mineral coupons that simulate certain seep conditions to investigate plausible in situ rates of carbonate dissolution associated with sulfur-oxidizing bacteria. Bioreactors inoculated with a sulfur-oxidizing bacterial strain, Celeribacter baekdonensis LH4, growing on aragonite coupons induced dissolution rates in sulfidic, heterotrophic, and abiotic conditions of 1773.97 (±324.35), 152.81 (±123.27), and 272.99 (±249.96) μmol CaCO3 • cm−2 • yr−1, respectively. Steep gradients in pH were also measured within carbonate-attached biofilms using pH-sensitive fluorophores. Together, these results show that the production of acidic microenvironments in biofilms of sulfur-oxidizing bacteria are capable of dissolving carbonate rocks, even under well-buffered marine conditions. Our results support the hypothesis that authigenic carbonate rock dissolution driven by lithotrophic sulfur-oxidation constitutes a previously unknown carbon flux from the rock reservoir to the ocean and atmosphere.

Extracting copper and cobalt from non-ferrous residues by iron- and sulfur-oxidizing bacteria

2021 ◽  
Author(s):  
Jianxing Sun ◽  
Wenxian Liu ◽  
Ruichang Tang ◽  
Haina Cheng ◽  
Ronghui Liu ◽  
...  
Keyword(s):  
Sulfur Oxidizing ◽  
Sulfur Oxidizing Bacteria
Sign in / Sign up

Export Citation Format

Share Document