〈1057〉 Biotechnology-Derived Articles—Total Protein Assay

2021 ◽  
Keyword(s):  
Total Protein ◽  
Protein Assay ◽  
Total Protein Assay

Prediction of Human Acute Toxicity by the Hep G2/24-hour/Total Protein Assay, with Protein Measurement by the CBQCA Method

2005 ◽  
Vol 33 (3) ◽  
pp. 207-213 ◽  
Author(s):  
Paul J. Dierickx
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Human Toxicity ◽  
Hep G2 ◽  
Vitro Assay ◽  
Protein Assay ◽  
Lowry Method ◽  
Total Protein Assay

In our previously described Hep G2/24-hour/total protein assay, protein levels were measured by using the Lowry method. This assay was the best acute in vitro assay for the prediction of human toxicity within the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) study. In order to increase the MEIC data-base with a wider range of chemicals, we were interested in introducing the more practical 3-(4-carboxybenzoyl)-quinoline-2-carboxaldehyde (CBQCA) method for the quantification of the total protein content. Therefore, we investigated whether the same good results for the prediction of acute human toxicity would be obtained with the CBQCA method. The cells were treated for 24 hours, then cytotoxicity was determined by measuring the total protein content with CBQCA. The results were quantified by using the PI50c: the concentration (in mM) of test compound required to reduce the total protein content measured with the CBQCA-method by 50% as compared to the control cells. The results were compared with the PI50, the corresponding value when the Lowry method was used. A relatively low correlation was observed between PI50 and PI50c, reflecting the large and unexpected, differences when using the two protein assays. However, when comparing the log PI50c with the human toxicity, a correlation coefficient of r2 = 0.761 ( n = 44) was obtained for exactly the same series of MEIC chemicals. This value is clearly higher than that for the Lowry method ( r2 = 0.695). Compared to the Lowry method originally used, the Hep G2/24-hour/CBQCA total protein assay has the additional important advantage that it can be very easily adapted for large-scale analyses with robotic systems, including the on-line calculation of the results.

Evaluation of the Hitachi 704 automatic analyzer.

1987 ◽  
Vol 33 (11) ◽  
pp. 2089-2092 ◽  
Author(s):  
E G Lentjes ◽  
G A Harff ◽  
E T Backer
Keyword(s):  
Lactate Dehydrogenase ◽  
Total Protein ◽  
Alanine Aminotransferase ◽  
Reference Method ◽  
Analytical Performance ◽  
Comparison Of Methods ◽  
Protein Assay ◽  
Automatic Analyzer ◽  
Total Protein Assay ◽  
Better Than

Abstract We evaluated the analytical performance of the Hitachi 704 automatic analyzer. The spectrophotometer showed a linearity of response at 340 nm up to 2.8 A. Photometric imprecision measured bichromatically at 340 and 376 nm was 0.49% at 0.16 A, 0.14% at 0.46 A, and 0.17% at 0.76 A. Imprecision of the sample probe was 0.4% for 5, 10, and 20 microL, and the volume delivered deviated -2.4%, -4.4%, and -4.2% from these preset volumes, respectively. Imprecision of the reagent probe over the range 50 to 500 microL ranged from 0.14% to 0.29%; volume delivered deviated from +1.7% to +4.4%). At equilibrium, the temperature in the cuvets was 29.8 (SD 0.05) degree C as measured by cresol red spectrophotometry. No sample carryover was detected. Reagent carryover was detected when a bilirubin assay was preceded by a total protein assay and when lactate dehydrogenase was measured after alanine aminotransferase. Imprecision for nine tests at three concentrations ranged from 1.1% to 4.4%. Comparison of methods with the SMAC II as reference method showed good results. Precision was better than reported for the Hitachi 705 automatic analyzer.

Standardize the serum albumin assay now: calibrate it to 60% of the serum total protein assay

2013 ◽  
Vol 51 (7) ◽  
Author(s):  
Hedwig C.M. Stepman ◽  
Dietmar Stöckl ◽  
Linde A.C. De Grande ◽  
Linda M. Thienpont
Keyword(s):  
Serum Albumin ◽  
Total Protein ◽  
Serum Total Protein ◽  
Protein Assay ◽  
Total Protein Assay

Spectrophotometric total protein assay with copper(II)–neocuproine reagent in alkaline medium

Talanta ◽  
2006 ◽  
Vol 68 (5) ◽  
pp. 1601-1609 ◽  
Author(s):  
K SOZGEN ◽  
S CEKIC ◽  
E TUTEM ◽  
R APAK
Keyword(s):  
Alkaline Medium ◽  
Total Protein ◽  
Protein Assay ◽  
Total Protein Assay

Simple Method for Correcting Total Protein in Plasma for Actual Fibrinogen Content

1992 ◽  
Vol 38 (11) ◽  
pp. 2221-2223 ◽  
Author(s):  
A J Bakker ◽  
J P Gorgels ◽  
J Draaisma ◽  
M Jongendijk ◽  
L Altena ◽  
...  
Keyword(s):  
Total Protein ◽  
Mean Difference ◽  
Serum Total Protein ◽  
Simple Method ◽  
Protein Assay ◽  
Reactive Protein ◽  
Actual Amount ◽  
The Mean ◽  
Mean Concentration ◽  
Total Protein Assay

Abstract Using plasma instead of serum for routine chemistry analyses has many advantages. To overcome the disadvantage of inclusively measuring fibrinogen in the plasma total protein assay without changing the clinical significance of the total protein assay, we investigated the possibility of subtracting the actual amount of fibrinogen from the plasma total protein. The correlation between serum and plasma total protein was excellent (plasma total protein = 0.989 x serum total protein + 6.7 g/L; r = 0.969; n = 131; mean difference = 5.55 g/L; P < 0.001). When the plasma total protein was corrected for the actual amount of fibrinogen, the correlation with serum total protein was equally good but the intercept was practically eliminated (corrected plasma total protein = 1.009 x serum total protein + 0.25 g/L; r = 0.985; n = 131; mean difference = 0.78 g/L; P = 0.47). The mean concentration of fibrinogen was 2.5 g/L (range: 1.38-3.62 g/L; n = 404) for blood donors, 3.6 g/L (n = 2707) for patients from the outpatient department, 4.6 g/L (n = 2023) for patients admitted to the hospital, and 6.6 g/L (n = 219) for patients whose concentration of C-reactive protein was > 50 mg/L. We conclude that the plasma total protein result should be corrected for the actual amount of fibrinogen.

scholarly journals HYALURONAN IN THE CEREBROSPINAL FLUID OF PATIENTS WITH SPINAL TUMOR

2006 ◽  
Vol 10 (03) ◽  
pp. 157-161
Author(s):  
Kenshi Sakayama ◽  
Teruki Kidani ◽  
Hiroyuki Tsuchiya ◽  
Nozomu Tanji ◽  
Yuji Watanabe ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Clinical Significance ◽  
Total Protein ◽  
Binding Protein ◽  
Spinal Tumor ◽  
Intramedullary Tumor ◽  
Histopathological Diagnosis ◽  
Tumor Site ◽  
Protein Assay ◽  
Extramedullary Tumor

The clinical significance of hyaluronan (HA) levels in the cerebrospinal fluid (CSF) of patients with spinal tumor (ST) was evaluated in order to clarify whether HA concentrations in the CSF of patients with ST differ according to such factors as the tumor site and histopathological diagnosis. CSF samples were obtained from 40 patients with ST who had undergone myelography and CSF examinations retrospectively. The HA levels were determined using a sandwich-binding protein assay. The total protein (TP) levels were also determined. The HA and TP concentrations in CSF were significantly higher in patients with extramedullary tumor than in patients with intramedullary tumor. There was a significant correlation between HA and TP concentrations in CSF patients with ST. A HA assay for CSF is therefore considered to be potentially useful for estimating the localization of ST.

A Cascade Microfluidic Device for High Quality Mitochondria Extraction

2015 ◽  
Author(s):  
Ching-Wen Li ◽  
Pao-Hsin Yen ◽  
Gou-Jen Wang
Keyword(s):  
Microfluidic Device ◽  
Complex I ◽  
Soft Lithography ◽  
Cell Damage ◽  
High Quality ◽  
Protein Assay ◽  
Lithography Process ◽  
Disease Therapy ◽  
Sds Page ◽  
Total Protein Assay

Recent researches have demonstrated that cells ingest mitochondrial by endocytosis to repair cell damage. This new mitochondrial therapy approach can be used for curing particular disease of neuropathy related diseases. Hence the obtainment of high quality and healthy mitochondria play an important role in mitochondrial based disease therapy. In this study, we propose a cascade microfluidic device for green extraction of healthy mitochondria. The geometry of the device was designed using the commercially available COMSOL package. Soft lithography process was than conducted to realize the device of PDMS. We used C2-GFP cells to demonstrate the efficiency of the proposed cascade microfluidic device. The total protein assay kit (complex I-V) was conducted to examine the extractive protein and the SDS page (Tom 20) was used for measuring the activity of the extracted mitochondria. Experimental results illustrate that the complex I-V expression of the extracted mitochondria by the proposed device is much higher than that of the extracted mitochondria by conventional kit. Furthermore, the results of the Tom 20 expression also demonstrate that our device is able to extract more amounts of mitochondria than the conventional kit.

Sign in / Sign up

Export Citation Format

Share Document