Simple Method for Correcting Total Protein in Plasma for Actual Fibrinogen Content

1992 ◽  
Vol 38 (11) ◽  
pp. 2221-2223 ◽  
Author(s):  
A J Bakker ◽  
J P Gorgels ◽  
J Draaisma ◽  
M Jongendijk ◽  
L Altena ◽  
...  
Keyword(s):  
Total Protein ◽  
Mean Difference ◽  
Serum Total Protein ◽  
Simple Method ◽  
Protein Assay ◽  
Reactive Protein ◽  
Actual Amount ◽  
The Mean ◽  
Mean Concentration ◽  
Total Protein Assay

Abstract Using plasma instead of serum for routine chemistry analyses has many advantages. To overcome the disadvantage of inclusively measuring fibrinogen in the plasma total protein assay without changing the clinical significance of the total protein assay, we investigated the possibility of subtracting the actual amount of fibrinogen from the plasma total protein. The correlation between serum and plasma total protein was excellent (plasma total protein = 0.989 x serum total protein + 6.7 g/L; r = 0.969; n = 131; mean difference = 5.55 g/L; P < 0.001). When the plasma total protein was corrected for the actual amount of fibrinogen, the correlation with serum total protein was equally good but the intercept was practically eliminated (corrected plasma total protein = 1.009 x serum total protein + 0.25 g/L; r = 0.985; n = 131; mean difference = 0.78 g/L; P = 0.47). The mean concentration of fibrinogen was 2.5 g/L (range: 1.38-3.62 g/L; n = 404) for blood donors, 3.6 g/L (n = 2707) for patients from the outpatient department, 4.6 g/L (n = 2023) for patients admitted to the hospital, and 6.6 g/L (n = 219) for patients whose concentration of C-reactive protein was > 50 mg/L. We conclude that the plasma total protein result should be corrected for the actual amount of fibrinogen.

Standardize the serum albumin assay now: calibrate it to 60% of the serum total protein assay

2013 ◽  
Vol 51 (7) ◽  
Author(s):  
Hedwig C.M. Stepman ◽  
Dietmar Stöckl ◽  
Linde A.C. De Grande ◽  
Linda M. Thienpont
Keyword(s):  
Serum Albumin ◽  
Total Protein ◽  
Serum Total Protein ◽  
Protein Assay ◽  
Total Protein Assay

Prediction of Human Acute Toxicity by the Hep G2/24-hour/Total Protein Assay, with Protein Measurement by the CBQCA Method

2005 ◽  
Vol 33 (3) ◽  
pp. 207-213 ◽  
Author(s):  
Paul J. Dierickx
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Human Toxicity ◽  
Hep G2 ◽  
Vitro Assay ◽  
Protein Assay ◽  
Lowry Method ◽  
Total Protein Assay

In our previously described Hep G2/24-hour/total protein assay, protein levels were measured by using the Lowry method. This assay was the best acute in vitro assay for the prediction of human toxicity within the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) study. In order to increase the MEIC data-base with a wider range of chemicals, we were interested in introducing the more practical 3-(4-carboxybenzoyl)-quinoline-2-carboxaldehyde (CBQCA) method for the quantification of the total protein content. Therefore, we investigated whether the same good results for the prediction of acute human toxicity would be obtained with the CBQCA method. The cells were treated for 24 hours, then cytotoxicity was determined by measuring the total protein content with CBQCA. The results were quantified by using the PI50c: the concentration (in mM) of test compound required to reduce the total protein content measured with the CBQCA-method by 50% as compared to the control cells. The results were compared with the PI50, the corresponding value when the Lowry method was used. A relatively low correlation was observed between PI50 and PI50c, reflecting the large and unexpected, differences when using the two protein assays. However, when comparing the log PI50c with the human toxicity, a correlation coefficient of r2 = 0.761 ( n = 44) was obtained for exactly the same series of MEIC chemicals. This value is clearly higher than that for the Lowry method ( r2 = 0.695). Compared to the Lowry method originally used, the Hep G2/24-hour/CBQCA total protein assay has the additional important advantage that it can be very easily adapted for large-scale analyses with robotic systems, including the on-line calculation of the results.

scholarly journals The Effect of Parenteral Infusion of Amino Acids in Burn Patient

2013 ◽  
Vol 24 (1) ◽  
pp. 12-15
Author(s):  
Kamrun Nahar ◽  
Zeba-un Naher ◽  
Matira Khanam ◽  
Shaheen Akhter ◽  
Tahmina Bashar ◽  
...  
Keyword(s):  
Amino Acid ◽  
Serum Albumin ◽  
Total Protein ◽  
Burn Injury ◽  
Burn Patients ◽  
Serum Total Protein ◽  
Female Ratio ◽  
Group I ◽  
The Mean ◽  
Group Ii

Adequate nutritional support may prevent weight loss  following severe burn injury. However, persistently low  levels of serum albumin, transferring and serum total  protein in burn patients have suggested that a protein  deficiency may continue to exist which is out of proportion  to energy requirements.  This interventional study cross sectional study was done in  the Department of Biochemistry, Bangabandhu Sheikh  Mujib Medical University (BSMMU), Dhaka, Bangladesh  during January 2008 to December 2008. A total of 40 acute  burn injury (within 24 hours of burn) patients of 20-45  years age with 15%-30% burn were selected for this study  as case. The study subjects were divided into two groups:  Group I represent superficial burn & Group II represents  deep burn.  The mean age of 28.35±6.81 years and 30.85±7.32 years in  group I and group II respectively. The number of male in  Group-I was 08 and Group-II was 08 and male female ratio  was 2:3. The mean serum total protein before infusion of  amino acid in Group-I was 55.31±3.58 g/L and in Group-II  was 52.01±2.26 g/L (p<0.001). The mean serum total  protein after infusion of amino acid in Group-I was  68.02±2.04 g/L and in Group-II was 61.86±2.49g/L  (p<0.001). The mean serum albumin before infusion of  amino acid in Group-I was 27.6±2.88 g/L and in Group-II  was 25.57±1.89 g/L (p<0.001). The mean serum albumin  after infusion of amino acid in Group-I was 22.29±3.50 g/L  and in Group-II was 19.83±2.86 g/L (p<0.001). In group-I,  serum total protein was increased by 22.98% after infusion  and in group-II, that was increased by 18.94% (p<0.01).  In group-I, serum albumin was decreased by 19.24% after  infusion and in group-II, that was decreased by 22.45%  (p<0.05). Serum total protein significantly increased after  infusion of amino acid but serum albumin significantly  decreased after infusion of amino acid. DOI: http://dx.doi.org/10.3329/medtoday.v24i1.14107 Medicine TODAY Vol.24(1) 2012 pp.12-15

Evaluation of the Hitachi 704 automatic analyzer.

1987 ◽  
Vol 33 (11) ◽  
pp. 2089-2092 ◽  
Author(s):  
E G Lentjes ◽  
G A Harff ◽  
E T Backer
Keyword(s):  
Lactate Dehydrogenase ◽  
Total Protein ◽  
Alanine Aminotransferase ◽  
Reference Method ◽  
Analytical Performance ◽  
Comparison Of Methods ◽  
Protein Assay ◽  
Automatic Analyzer ◽  
Total Protein Assay ◽  
Better Than

Abstract We evaluated the analytical performance of the Hitachi 704 automatic analyzer. The spectrophotometer showed a linearity of response at 340 nm up to 2.8 A. Photometric imprecision measured bichromatically at 340 and 376 nm was 0.49% at 0.16 A, 0.14% at 0.46 A, and 0.17% at 0.76 A. Imprecision of the sample probe was 0.4% for 5, 10, and 20 microL, and the volume delivered deviated -2.4%, -4.4%, and -4.2% from these preset volumes, respectively. Imprecision of the reagent probe over the range 50 to 500 microL ranged from 0.14% to 0.29%; volume delivered deviated from +1.7% to +4.4%). At equilibrium, the temperature in the cuvets was 29.8 (SD 0.05) degree C as measured by cresol red spectrophotometry. No sample carryover was detected. Reagent carryover was detected when a bilirubin assay was preceded by a total protein assay and when lactate dehydrogenase was measured after alanine aminotransferase. Imprecision for nine tests at three concentrations ranged from 1.1% to 4.4%. Comparison of methods with the SMAC II as reference method showed good results. Precision was better than reported for the Hitachi 705 automatic analyzer.

Reference values for fructosamine concentrations in children's sera: influence of protein concentration, age, and sex.

1988 ◽  
Vol 34 (12) ◽  
pp. 2444-2447 ◽  
Author(s):  
J De Schepper ◽  
M P Derde ◽  
P Goubert ◽  
F Gorus
Keyword(s):  
Reference Values ◽  
Protein Concentration ◽  
Reference Intervals ◽  
Mean Value ◽  
Age Dependency ◽  
Serum Total Protein ◽  
Normal Children ◽  
Glycated Protein ◽  
The Mean ◽  
Mean Concentration

Abstract Fructosamine and protein (total and fractionated) were measured in the serum of 170 normal children, ages two weeks to 15 years. The mean fructosamine concentration was 2.12 mmol/L, 5% lower than the mean value observed for adults. We observed no sex-related difference in fructosamine values, but saw a pronounced age dependency of reference values. For children younger than three years, the mean concentration of fructosamine was 15% lower than in adults, but glycated protein concentrations increased with age, reaching essentially adult values by age six years. Expressing fructosamine concentrations per gram of serum total protein or of albumin weakened the influence of age, but did not eliminate it completely. We report reference intervals for fructosamine concentrations in children's sera.

Spectrophotometric total protein assay with copper(II)–neocuproine reagent in alkaline medium

Talanta ◽  
2006 ◽  
Vol 68 (5) ◽  
pp. 1601-1609 ◽  
Author(s):  
K SOZGEN ◽  
S CEKIC ◽  
E TUTEM ◽  
R APAK
Keyword(s):  
Alkaline Medium ◽  
Total Protein ◽  
Protein Assay ◽  
Total Protein Assay

scholarly journals Evaluation of high sensitivity C-reactive protein assay in cerebrospinal fluid on the Dimension RxL analyzer

2012 ◽  
Vol 2 (1) ◽  
pp. 13-16
Author(s):  
Jozo Ćorić ◽  
Aleksandra Pašić ◽  
Mirsad Panjeta ◽  
Jasminka Mujić
Keyword(s):  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
High Sensitivity ◽  
Acute Bacterial Meningitis ◽  
Control Group ◽  
C Reactive Protein ◽  
Protein Assay ◽  
Reactive Protein ◽  
The Mean ◽  
Low Sensitivity

Introduction: Low sensitivity and specificity in traditional laboratory tests became insufficient for accurate diagnostics and initiation of proper treatment of patients infected with bacterial meningitis. High sensitivity C reactive protein (hsCRP) may be an appropriate supplement for rapid diagnosis of bacterial meningitis. The subject of our investigation was the determination of C- reactive protein in cerebrospinal fluid (CSF) duringacute bacterial meningitis.Methods: HsCRP was analysed by a sensitive immunoturbidimetric assay using the Dimension RxL analyser (Siemens). Cerebrospinal fluid concentrations of C-reactive protein have been measured in 20 patients(age range,1 to 50 years) presenting with acute bacterial meningitis and also in a non-infected, non-inflamed control group (n=25).Results: The accuracy and precision of the method proved to be satisfactory. Repeatability of serial sampling for hsCRP described by coefficient of variation were CV=2.1-4.5%. This assay hsCRP in cerebrospinal fluid demonstrates adequate performance characteristics for routine clinical use. Elevated levels of CRP were found in 95% patients with bacterial meningitis. The mean CRP value in 25 uninfected control group was 0.25 mg/L (range 0.10-0.55). The mean CRP for patients with bacterial meningitis was 21.4 mg/L (range 0.40-100).Conclusions: A sensitive assay for CRP in CSF would be an useful adjunct to conventional investigation of acute infective meningitis.

scholarly journals C-reactive protein levels in acute pancreatitis and its clinical significance

2019 ◽  
Vol 6 (9) ◽  
pp. 3328
Author(s):  
Juthika Abhijit Deherkar ◽  
Ayush Pandey ◽  
Shahaji Deshmukh
Keyword(s):  
Acute Pancreatitis ◽  
C Reactive Protein ◽  
Simple Method ◽  
Protein Levels ◽  
Reactive Protein ◽  
Assessment And Monitoring ◽  
The Mean ◽  
Common Problems ◽  
Serum Crp ◽  
Comparative Prospective Study

Background: Acute pancreatitis is one of the most common problems faced by surgeon in their practice. Alcohol being one of the most important etiology in country like India. The most common line of management has always been conservative until and unless surgery is indicated for its complications. Till date amylase and lipase have been used as diagnostic tool for it however certain prognostic tools like CRP are still under evaluation. Thus we have made an attempt to evaluate its significance as a prognostic tool in this study.Methods: A hospital based observational comparative prospective study was done with 100 patients to measure C-reactive protein (CRP) levels in patients of acute pancreatitis and evaluate if CRP levels predict the severity of pancreatitis.Results: The mean serum CRP level of patients with Ranson’s score <3 was significantly higher as compared to mean serum CRP level of patients with Ranson’s score ≥3 (10.54±5.00 mg/l vs 7.29±3.94 mg/l). There was significant association of serum CRP and Ranson’s score of patients.Conclusions: The rapid response of CRP to changes in the intensity of the inflammatory stimulus suggests that it might be valuable in the assessment and monitoring of acute pancreatitis. It was observed in our study that measurement of CRP level is a simple method to assess the severity of disease.

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