Standardize the serum albumin assay now: calibrate it to 60% of the serum total protein assay

Abstract Using plasma instead of serum for routine chemistry analyses has many advantages. To overcome the disadvantage of inclusively measuring fibrinogen in the plasma total protein assay without changing the clinical significance of the total protein assay, we investigated the possibility of subtracting the actual amount of fibrinogen from the plasma total protein. The correlation between serum and plasma total protein was excellent (plasma total protein = 0.989 x serum total protein + 6.7 g/L; r = 0.969; n = 131; mean difference = 5.55 g/L; P < 0.001). When the plasma total protein was corrected for the actual amount of fibrinogen, the correlation with serum total protein was equally good but the intercept was practically eliminated (corrected plasma total protein = 1.009 x serum total protein + 0.25 g/L; r = 0.985; n = 131; mean difference = 0.78 g/L; P = 0.47). The mean concentration of fibrinogen was 2.5 g/L (range: 1.38-3.62 g/L; n = 404) for blood donors, 3.6 g/L (n = 2707) for patients from the outpatient department, 4.6 g/L (n = 2023) for patients admitted to the hospital, and 6.6 g/L (n = 219) for patients whose concentration of C-reactive protein was > 50 mg/L. We conclude that the plasma total protein result should be corrected for the actual amount of fibrinogen.

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Prediction of Human Acute Toxicity by the Hep G2/24-hour/Total Protein Assay, with Protein Measurement by the CBQCA Method

Alternatives to Laboratory Animals ◽

10.1177/026119290503300304 ◽

2005 ◽

Vol 33 (3) ◽

pp. 207-213 ◽

Cited By ~ 4

Author(s):

Paul J. Dierickx

Keyword(s):

Protein Content ◽

Total Protein ◽

Total Protein Content ◽

Human Toxicity ◽

Hep G2 ◽

Vitro Assay ◽

Protein Assay ◽

Lowry Method ◽

Total Protein Assay

In our previously described Hep G2/24-hour/total protein assay, protein levels were measured by using the Lowry method. This assay was the best acute in vitro assay for the prediction of human toxicity within the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) study. In order to increase the MEIC data-base with a wider range of chemicals, we were interested in introducing the more practical 3-(4-carboxybenzoyl)-quinoline-2-carboxaldehyde (CBQCA) method for the quantification of the total protein content. Therefore, we investigated whether the same good results for the prediction of acute human toxicity would be obtained with the CBQCA method. The cells were treated for 24 hours, then cytotoxicity was determined by measuring the total protein content with CBQCA. The results were quantified by using the PI50c: the concentration (in mM) of test compound required to reduce the total protein content measured with the CBQCA-method by 50% as compared to the control cells. The results were compared with the PI50, the corresponding value when the Lowry method was used. A relatively low correlation was observed between PI50 and PI50c, reflecting the large and unexpected, differences when using the two protein assays. However, when comparing the log PI50c with the human toxicity, a correlation coefficient of r2 = 0.761 ( n = 44) was obtained for exactly the same series of MEIC chemicals. This value is clearly higher than that for the Lowry method ( r2 = 0.695). Compared to the Lowry method originally used, the Hep G2/24-hour/CBQCA total protein assay has the additional important advantage that it can be very easily adapted for large-scale analyses with robotic systems, including the on-line calculation of the results.

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〈1057〉 Biotechnology-Derived Articles—Total Protein Assay

10.31003/uspnf_m863_02_01 ◽

2021 ◽

Keyword(s):

Total Protein ◽

Protein Assay ◽

Total Protein Assay

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The Effect of Parenteral Infusion of Amino Acids in Burn Patient

Medicine Today ◽

10.3329/medtoday.v24i1.14107 ◽

2013 ◽

Vol 24 (1) ◽

pp. 12-15

Author(s):

Kamrun Nahar ◽

Zeba-un Naher ◽

Matira Khanam ◽

Shaheen Akhter ◽

Tahmina Bashar ◽

...

Keyword(s):

Amino Acid ◽

Serum Albumin ◽

Total Protein ◽

Burn Injury ◽

Burn Patients ◽

Serum Total Protein ◽

Female Ratio ◽

Group I ◽

The Mean ◽

Group Ii

Adequate nutritional support may prevent weight loss following severe burn injury. However, persistently low levels of serum albumin, transferring and serum total protein in burn patients have suggested that a protein deficiency may continue to exist which is out of proportion to energy requirements. This interventional study cross sectional study was done in the Department of Biochemistry, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh during January 2008 to December 2008. A total of 40 acute burn injury (within 24 hours of burn) patients of 20-45 years age with 15%-30% burn were selected for this study as case. The study subjects were divided into two groups: Group I represent superficial burn & Group II represents deep burn. The mean age of 28.35±6.81 years and 30.85±7.32 years in group I and group II respectively. The number of male in Group-I was 08 and Group-II was 08 and male female ratio was 2:3. The mean serum total protein before infusion of amino acid in Group-I was 55.31±3.58 g/L and in Group-II was 52.01±2.26 g/L (p<0.001). The mean serum total protein after infusion of amino acid in Group-I was 68.02±2.04 g/L and in Group-II was 61.86±2.49g/L (p<0.001). The mean serum albumin before infusion of amino acid in Group-I was 27.6±2.88 g/L and in Group-II was 25.57±1.89 g/L (p<0.001). The mean serum albumin after infusion of amino acid in Group-I was 22.29±3.50 g/L and in Group-II was 19.83±2.86 g/L (p<0.001). In group-I, serum total protein was increased by 22.98% after infusion and in group-II, that was increased by 18.94% (p<0.01). In group-I, serum albumin was decreased by 19.24% after infusion and in group-II, that was decreased by 22.45% (p<0.05). Serum total protein significantly increased after infusion of amino acid but serum albumin significantly decreased after infusion of amino acid. DOI: http://dx.doi.org/10.3329/medtoday.v24i1.14107 Medicine TODAY Vol.24(1) 2012 pp.12-15

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Correlation of nutrition-associated parameters with non-relapse mortality in allogeneic hematopoietic stem cell transplantation

Annals of Hematology ◽

10.1007/s00277-021-04736-0 ◽

2021 ◽

Author(s):

Judith Schaffrath ◽

Tanja Diederichs ◽

Susanne Unverzagt ◽

Maxi Wass ◽

Ulrike Gläser ◽

...

Keyword(s):

Multivariate Analysis ◽

Stem Cell ◽

Nutritional Status ◽

Stem Cell Transplantation ◽

Serum Albumin ◽

Cell Transplantation ◽

Total Protein ◽

Serum Total Protein ◽

Hematopoietic Stem ◽

Median Serum

AbstractOutcome of allogeneic stem cell transplantation (alloSCT) is hampered by substantial non-relapse mortality (NRM). Given its impact on organ function and immune response, the nutritional status has been suggested as relevant for NRM. We aimed to evaluate the association of NRM with nutritional status prior to alloSCT and in the post-SCT course. In a retrospective single-center study, we analyzed 128 alloSCTs. Besides standard characteristics, nutrition-associated parameters BMI, serum total protein, and serum albumin were recorded before conditioning and at various time points after alloSCT. Association with NRM was evaluated by univariate and multivariate survival analysis. The cohort comprised patients with a median BMI of 26 kg/m2 (16.7–46.9 kg/m2), median serum total protein of 59 g/l (41–77 g/l), and serum albumin of 36 g/l (22–46 g/l) before SCT. NRM at d+100 was 14.8% and at 1 year 26.6%. Prior to SCT, only serum albumin deficiency was associated with increased NRM (p = .010) in multivariate analysis. After SCT (d+30 and d+100), all nutrition-associated parameters decreased (p < .002), but no association of deteriorating nutritional status with NRM was found. In multivariate analysis, serum albumin (p = .03) and severe albumin deficiency (p = .02) correlated with NRM at d+30 and d+100, while BMI and serum total protein did not. In our study, albumin deficiency, particularly prior to alloSCT, shows a strong correlation with NRM. This finding may add to monitoring, risk evaluation, and counseling of patients and serve as a rational for interventions to improve the nutritional status in patients undergoing SCT.

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Evaluation of the Hitachi 704 automatic analyzer.

Clinical Chemistry ◽

10.1093/clinchem/33.11.2089 ◽

1987 ◽

Vol 33 (11) ◽

pp. 2089-2092 ◽

Cited By ~ 7

Author(s):

E G Lentjes ◽

G A Harff ◽

E T Backer

Keyword(s):

Lactate Dehydrogenase ◽

Total Protein ◽

Alanine Aminotransferase ◽

Reference Method ◽

Analytical Performance ◽

Comparison Of Methods ◽

Protein Assay ◽

Automatic Analyzer ◽

Total Protein Assay ◽

Better Than

Abstract We evaluated the analytical performance of the Hitachi 704 automatic analyzer. The spectrophotometer showed a linearity of response at 340 nm up to 2.8 A. Photometric imprecision measured bichromatically at 340 and 376 nm was 0.49% at 0.16 A, 0.14% at 0.46 A, and 0.17% at 0.76 A. Imprecision of the sample probe was 0.4% for 5, 10, and 20 microL, and the volume delivered deviated -2.4%, -4.4%, and -4.2% from these preset volumes, respectively. Imprecision of the reagent probe over the range 50 to 500 microL ranged from 0.14% to 0.29%; volume delivered deviated from +1.7% to +4.4%). At equilibrium, the temperature in the cuvets was 29.8 (SD 0.05) degree C as measured by cresol red spectrophotometry. No sample carryover was detected. Reagent carryover was detected when a bilirubin assay was preceded by a total protein assay and when lactate dehydrogenase was measured after alanine aminotransferase. Imprecision for nine tests at three concentrations ranged from 1.1% to 4.4%. Comparison of methods with the SMAC II as reference method showed good results. Precision was better than reported for the Hitachi 705 automatic analyzer.

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A Study of Factors that Contribute to the Severe Progression of Peritonsillar Abscess

International Journal of Practical Otolaryngology ◽

10.1055/s-0040-1702999 ◽

2020 ◽

Vol 03 (01) ◽

pp. e1-e5

Author(s):

Naoya Fujikawa ◽

Taku Yamashita

Keyword(s):

Blood Glucose ◽

Blood Cell ◽

Serum Albumin ◽

Cell Count ◽

White Blood Cell Count ◽

White Blood Cell ◽

Total Protein ◽

Blood Cell Count ◽

Serum Total Protein ◽

Protein Serum

Abstract Background Based on the background factors and results of blood tests in patients with peritonsillar abscess, we investigated the factors involved in the prolongation of hospitalization and progression to deep neck abscess of these patients. Objective and Methods We studied the data of 113 patients with peritonsillar abscesses (including those in whom the condition progressed to deep neck abscesses) who were hospitalized at our department during the 3-year period from August 2014 to August 2017. Data of patients who needed hospitalized treatment for 7 days or more, including the age, body mass index (BMI), sickness period, white blood cell count, serum C-reactive protein (CRP), serum total protein, serum albumin, blood glucose, blood HbA1c, and estimated glomerular filtration rate (eGFR) at the first examination, and the neck factors in the cases that progressed to deep cervical abscess were examined using t-tests. Results The blood test items and background factors at the first visit were compared according to the length of hospitalization. The results revealed that prolonged hospitalization was associated with higher values of age, peripheral blood white blood cell count, serum CRP value and blood glucose, and lower values of serum total protein, serum albumin, and eGFR. Conclusions Factors that were associated with a longer hospital stay in this study were relatively high values of age, peripheral blood white blood cell count, blood glucose and serum CRP, and relatively low values of the serum total protein, serum albumin, and eGFR. The disease duration, BMI, and HbA1c were not identified as significant factors. Stepwise multiple regression analysis to identify factors associated with prolonged hospitalization identified the blood glucose and serum albumin levels measured at the first visit as significant predictors of a prolonged hospital stay and higher severity of the illness. Our findings suggested the importance of measuring these factors for predicting the progression to deep cervical abscess.

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Comparison of Serum Total Protein and Serum Albumin after Infusion of Amino Acid of the 15%-30% Burn Patients

Journal of Science Foundation ◽

10.3329/jsf.v9i1-2.14653 ◽

2013 ◽

Vol 9 (1-2) ◽

pp. 109-113

Author(s):

N Kamrun ◽

Zeba Un-Naher ◽

M Ali ◽

T Bashar ◽

MI Arslan

Keyword(s):

Amino Acid ◽

Serum Albumin ◽

Total Protein ◽

Burn Injury ◽

Burn Patients ◽

Amino Acid Supplementation ◽

Serum Total Protein ◽

Cross Sectional ◽

Group I ◽

Group Ii

The aim of the study to find out the ratio of raise of serum total protein and serum albumin during amino acid supplementation in burn patients. This interventional study cross sectional study was done in the Department of Biochemistry, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh during January 2008 to December 2008. A total of 40 acute burn injury (within 24 hours of burn) patients of 20-45 years age with 15%-30% burn were selected for this study as case. The study subjects were divided into two groups: Group I represent superficial burn & Group II represents deep burn. In group-I, serum albumin was decreased by 19.24% after infusion and in group-II, that was decreased by 22.45% (p<0.05). Serum total protein significantly increased after infusion of amino acid but serum albumin significantly decreased after infusion of amino acid. DOI: http://dx.doi.org/10.3329/jsf.v9i1-2.14653 J. Sci. Foundation, 9(1&2): 109-113, June-December 2011

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Serum Total Protein, Albumin, Globulin, and Prealbumin in Acne Patients

10.21203/rs.3.rs-122981/v1 ◽

2020 ◽

Author(s):

Hao Jiang ◽

Changyi Li ◽

bin wei ◽

Qiang Wang ◽

Qinggao Wang ◽

...

Keyword(s):

Serum Albumin ◽

Total Protein ◽

Serum Levels ◽

Control Group ◽

Healthy Controls ◽

Serum Globulin ◽

Serum Total Protein ◽

Protein Levels ◽

Healthy Control ◽

Age And Sex

Abstract Background/Objectives: This study was designed to investigate serum protein levels in acne patients.Method: Acne patients (n=362) and healthy volunteers (n=272) were matched in terms of both age and sex. Serum levels were measured.Results: Among the 362 acne patients and 272 age- and sex-matched healthy controls, serum albumin levels in female acne patients were lower than in the healthy controls (P < 0.05), serum albumin levels in male acne patients were lower than in the healthy controls (P < 0.01). Additionally, serum globulin and total protein levels were significantly lower in acne patients than in the healthy control group (P < 0.01). Serum levels of prealbumin were significantly lower in female acne patients than in the control group (P < 0.05). Finally, the severity of female and male acne patients was negatively correlated with serum total protein, albumin, globulin, and prealbumin levels. Conclusions: The results of this study suggested that acne patients are potentially accompanied with protein malnutrition.

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Study of thyroid profile in malnourished children (6 months - 5 years) admitted in the nutritional rehabilitation centre and paediatric ward NSCB Medical College Jabalpur, India

International Journal of Contemporary Pediatrics ◽

10.18203/2349-3291.ijcp20181545 ◽

2018 ◽

Vol 5 (3) ◽

pp. 1072

Author(s):

Monica Lazarus ◽

Alok Kumar Kashyap ◽

Rahul Borkar ◽

Manish Ajmariya

Keyword(s):

Thyroid Hormone ◽

Serum Albumin ◽

Total Protein ◽

Tertiary Care ◽

Anthropometric Measurement ◽

Care Hospital ◽

Serum Total Protein ◽

Major Health Problem ◽

Malnourished Children ◽

Thyroid Profile

Background: Globally, PEM continues to be a major health problem in developing countries and the most important risk factor for illnesses and death especially among young children. The research was conducted to study the effect of thyroid profile in malnourished children (6 month-5 years) admitted in tertiary care center in Central India.Methods: A cross sectional hospital based observational study was carried out in tertiary care hospital NSCB MCH Jabalpur. 80 malnourished children were included in the study. The cases were categorized into moderate and severe malnutrition as per WHO classification. Detailed clinical assessment of nutritional status followed by anthropometric measurement (weight for height) was recorded in predesigned proforma. Triiodothyronine (T3), Thyroxine (T4), thyroid stimulating hormone (TSH), serum total protein and serum albumin were estimated. The parameters were compared among SAM and MAM groups using appropriate tool.Results: Mean serum protein, serum albumin, T3, T4 were significantly low in SAM group, when compared to the MAM group (p <0.001). while level of TSH was not found significant among SAM and MAM groups.Conclusions: Malnutrition is associated with decrease level of thyroid hormone levels and were positively correlated with serum total protein and albumin levels. The decrease level of thyroid hormone may have a contributory role in retarded growth and development.

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