scholarly journals Phytoremedial effect of fruit extract of Moringa oleifera on alloxan induced diabetic model in Swiss albino mice

2021 ◽  
Vol 13 (4) ◽  
pp. 1420-1429
Author(s):  
Roushan Kumari ◽  
Arun Kumar Singh ◽  
Ranjit Kumar ◽  
Arun Kumar
Keyword(s):  
Glucose Level ◽  
Moringa Oleifera ◽  
Histopathological Examination ◽  
Diabetic Control ◽  
Control Group ◽  
Histopathological Study ◽  
Marker Enzyme ◽  
Fruit Extract ◽  
Group I ◽  
Diabetic Model

Diabetes is a metabolic disorder and global health issue. It arises because of an absolute or relative insulin deficiency that causes hyperglycemia. The study aimed to assess the antihyperglycemic, hepatoprotective, and renal protective effects of ethanolic fruit extract of Moringa oleifera, on alloxan-induced diabetic mice. Four mice were assigned to each group.  Alloxan was injected at the dose of 10mg/kg/body weight intraperitoneally to make the diabetic model in mice. Control and diabetic control mice received drinking water as a placebo, while the diabetic model mice group was administered with ethanolic extract of moringa fruit at the dose of 150mg/kg/bodyweight for 12 weeks. After that, animals were sacrificed, and their blood and tissue samples were collected for biochemical and histopathological examination. The glucose level markedly (p<0.0001) increased many folds in Group I (80.73± 1.24 to 221.5±13.4) and Group II (80.73 ± 1.24 from to 221.9±6.88). The level of insulin markedly (P< 0.0001) decreased in both groups (6.8±0.42 to1.378±0.19) and (6.8±0.42 to1.138±0.08) respectively. Serum hepatic and renal marker enzymes increased in the diabetic group of mice. Glucose level was meaningfully (p<0.0001) decreased in the M. oleifera administered group while serum insulin level significantly (p<0.0001) increased. The level of liver marker enzyme and renal marker also decreased as compared to the diabetic control group. Histopathological study revealed that alloxan treatment caused damage to the liver, kidney, and pancreatic tissues while the M. oleifera administered group exhibited significant improvement in the architecture of the liver, kidney, and pancreas. Hence, M. oleifera has great potential to rejuvenate the damaged tissue and consequently can restore all the serum enzymatic and hormonal parameters.

scholarly journals PHOENIX DACTYLIFERA FRUIT EXTRACT AMELIORATES ALTERED BIOCHEMICAL PARAMETERS IN STREPTOZOTOCIN-INDUCED DIABETES MELLITUS ALBINO RATS

2020 ◽  
pp. 27-31
Author(s):  
OGO A. O. ◽  
ABAH S. F. ◽  
INALEGWU B. ◽  
ERU U. E.
Keyword(s):  
Diabetes Mellitus ◽  
Biochemical Parameters ◽  
Date Palm ◽  
Diabetic Control ◽  
Alternative Methods ◽  
Control Group ◽  
Fruit Extract ◽  
Palm Fruit ◽  
Positive Effects ◽  
Group I

Objective: Consistent projections have indicated a steady increase in the global burden of diabetes mellitus. Given the increased cost in conventional management of the condition in addition to reported side effects and cost of orthodox management, attention is shifting to the use of alternative methods such as plants materials with dual benefits as food and medicine. Thus, this study was designed to investigate the effect of date palm fruit on some biochemical parameters in streptozotocin-induced wistar rats. Methods: Animals were divided into 5 groups of 6 rats each (I as normal control, II as diabetic control and 3-5 as extract-treated groups) maintained for 14 d. At the end of the treatment, the animals were fasted overnight, then sacrificed and blood samples collected for analysis of biochemical parameters (including blood glucose, blood lipids and enzymes). Results: The results show that treatment of diabetic animals with extract of date palm fruit show a significant (P<0.05) reduction in glucose levels in groups II–V compared to group I. Similar positive effects were observed in the levels of lipids and enzymes in treated groups compared to diabetic control group II. A GC-MS analysis of the fraction of the fruit extract revealed some bioactive compounds that may be responsible for the effects exhibited in the study. Conclusion: These findings which demonstrate the ameliorative effect on hyperglycemia and hyperlipidemia, further support the use of date palm fruit as a nutraceutical agent.

scholarly journals A Comparative Study of the Effect of Lignocaine and Tramadol on Healing of Infected wound in Wistar Rats

2019 ◽  
Vol 12 (3) ◽  
pp. 1491-1496
Author(s):  
Smita Shenoy ◽  
Abhijna Ballal R ◽  
Praveen S E. Kumar ◽  
Mamatha Ballal ◽  
Sohan Bangera ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Breaking Strength ◽  
Histopathological Examination ◽  
Control Group ◽  
Histopathological Study ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Group Ii ◽  
Incision Wound

The study was conducted to assess the effect of lignocaine and tramadol on healing of Staphylococcus aureus infected incision wound in rats. Incision wounds were created in three groups consisting of six rats in each group. The rats were infected with Staphylococcus aureus inoculum and the incision was closed. The rats were treated according to their group – Group I (Normal saline), Group II (2% Lignocaine) and Group III (5% Tramadol). All the drugs were applied topically, once a day for 10 days. The parameters measured to evaluate the wound healing was breaking strength, Colony Forming Units/ mL (CFU/ mL) of the tissue and histopathological examination. Data analysis was by one-way (Analysis of Variance) ANOVA followed by post hoc Tukey’s test. A significant difference (p<0.05 vs control) in the breaking strength and CFU/mL of tissue was observed between the control Group I and the test groups, Group II (2%lignocaine) and Group III (5% tramadol) when the drugs were used topically. Histopathological study did not show much difference in the morphology between the control and other groups, it revealed disorganised collagen fibres. This study suggests that lignocaine and tramadol do not impede healing of infected incision wound.

Anti-diabetic activity of diphenhydramine in diabetic rats

2020 ◽  
Vol 11 (4) ◽  
pp. 5067-5070
Author(s):  
Pang Jyh Chayng ◽  
Nurul Ain ◽  
Kaswandi Md Ambia ◽  
Rahim Md Noah
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Group Iv ◽  
Diabetic Rat ◽  
Control Group ◽  
Group I

The purpose of this project is to study the anti-diabetic effect of on a diabetic rat model. A total of Twenty male Sprague rats were used and it randomly distributed into four groups which are Group I: , Group II: negative control, Group III: and Group IV: and . In diabetic model were induced with via injection at the dosage of 65mg/kg. and FBG (Fasting Blood Glucose) level of diabetic rats were assessed every three days. Blood was collected via cardiac puncture at day 21 after the induction of treatment. Insulin level of the rats was assessed with the Mercodia Rat Insulin ELISA kit. FBG level of group I (12.16 ±3.96, p&lt;0.05) and group IV (11.34 ±3.67, p&lt;0.05) were significantly decreased. Meanwhile, the for all rats did not show any significant increase. However, the insulin level was escalated in group IV (0.74+0.25, p&lt;0.05) significantly. The present study shows that the and the combination of and lowered blood glucose level and enhanced insulin secretion.

Antidiabetic and Hypolipidemic Effects of Extracts of Gymnema Sylvestre in Streptozotocin Induced Type I Diabetes in Rats

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
Nishtha R. Mahida ◽  
G. . Mandali ◽  
Vijaysinh V. Sindha ◽  
S. K. Raval
Keyword(s):  
Blood Glucose ◽  
Lipid Profile ◽  
Type I Diabetes ◽  
Histopathological Examination ◽  
Diabetic Control ◽  
Hypolipidemic Activity ◽  
Control Group ◽  
Type I ◽  
Gymnema Sylvestre ◽  
Altered Glucose

Gymnema sylvestre of the family Asclepiadaceae is one of the most important medicinal plants of the central eco-region. It is popularly known as Gurmar, which means “sugar killer”. Extract of leaves is reported to have tannins, gum, flavonoids, proteins and saponins. It has displayed a wide array of pharmacological activities. This study was aimed to investigate the antidiabetic and hypolipidemic effects of Gymnema sylvestre extract in experimentally induced diabetes in rats. Diabetes was produced in adult Wistar rats with single dose of streptozotocin (STZ) @ 60 mg/kg b.wt. intraperitoneally. After the confirmation of diabetes on 7th day (sugar >200 mg/dl), alcoholic and aqueous extracts of G. sylvestre (400 mg/kg) were administered orally to the experimental rats from 8th day and continued for 42 days thereafter. The antidiabetic and hypolipidemic activity was estimated by measuring blood glucose, lipid profile and histopathological examination of various tissues from all the groups. Administration of STZ resulted in a significant (p less than 0.01) increase in blood glucose and lipid profile and histopathological alterations in Diabetic control group as compared to healthy control group. Gymnema treatment demonstrated significant (p less than 0.01) antidiabetic effect indicated by restoration of blood glucose compared to STZ control group. The study concluded that extracts of Gymnema sylvestre improved the altered glucose and lipid profile in diabetic rats, suggesting that the Gymnema Sylvestre extracts exhibit the antidiabetic and hypolipidemic activity.

INVESTIGATION OF THE EFFECT OF BLACK SEED OIL ON DIABETES-INDUCED - TESTICULAR DAMAGE: A HISTOPATHOLOGICAL STUDY

2021 ◽  
pp. 29-31
Author(s):  
Seval Kaya ◽  
Yusuf Nergiz ◽  
Firat Asir
Keyword(s):  
Tissue Damage ◽  
Seed Oil ◽  
Histopathological Examination ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Histopathological Study ◽  
Diabetes Group ◽  
Black Seed ◽  
Black Seed Oil

In this study, it was aimed to investigate the protective effect of black seed oil against testicular tissue damage in diabetic rats. A total of 18 male rats were divided into 3 groups, including 6 rats in each group.Groups; control (n=6), diabetes (n=6), diabetes + black seed oil (n=6). A single dose of 45 mg / kg streptozocine (STZ) was injected intraperitoneally to induce diabetes. Diabetes + Black seed oil group: For 56 days, 2.5 ml / kg of black seed oil was administered orally to rats.The rats were sacriced at the end of 56 days. Testicular tissues were taken for routine parafn tissue processing for histopathological examination. Parafn sections were stained with Hematoxylin-Eosin and PAS and examined under a light microscope. Atrophy and degeneration were observed in the seminiferous tubules of diabetic group. Histology of black seed oil group sections were similar to that of control group. A signicant difference was found between the black seed oil group and the diabetes group in terms of blood glucose values. As a result, we think that Black Seed Oil ameliorates to the tissue damage caused by diabetes and the decrease in blood sugar value.

Impact of supplementing commercial rabbit pellets with fresh Moringa oleifera leaves on semen characteristics in rabbit bucks under tropical climate in Trinidad

2021 ◽  
Author(s):  
Krishna Mohan Kumar
Keyword(s):  
Sperm Motility ◽  
Moringa Oleifera ◽  
Sperm Count ◽  
Control Group ◽  
Computer Assisted ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Moringa Oleifera Leaves ◽  
The Impact

Objective This study aimed to evaluate the impact of the dietary supplement of Moringa oleifera leaves (MOL) on semen quality and characteristics in rabbits. Methods Eighteen (n=18) breeding bucks of New Zealand white, of similar age group, were used for the study. Three feeding regimes, (i) 100% commercial rabbit pellets (CRP)-Group I (ii) 90% CRP + 10% fresh MOL on a dry matter (DM) basis – Group II and (iii) 80% CRP + 20% fresh MOL on a DM basis – Group III, were adopted and the trial continued for 21 days. After adaptation to the diet, semen was collected from each buck and subjected to evaluation using a computer-assisted semen analyser. Results In Group III, the sperm count, normal sperm morphology, and sperm motility increased (52.0%) in comparison with the control (Group I; 50.1%). The inclusion of 20% Moringa oliefera in the diet (Group III) caused a significant increase (P<0.05) in semen concentration (Control =136.2 M/mL; Group III=297.2 M/mL). There was no significant difference (P>0.05) in sperm motility and semen volume among the groups. Conclusion The results suggest that supplementing commercial rabbit pellets with 20% fresh Moringa oliefera leaves on a DM basis can improve the quality and characteristics of semen in breeding bucks.

scholarly journals CARDIOPROTECTIVE EFFECT OF MENTHA LONGIFOLIA AGAINST CYCLOPHOSPHAMIDE INDUCED CARDIOTOXICITY IN RATS: A BIOCHEMICAL, ELECTROCARDIOGRAPHIC AND HISTOPATHOLOGICAL STUDY

2016 ◽  
Vol 8 (9) ◽  
pp. 214 ◽  
Author(s):  
Ayshath Afroos Shahana A.r. ◽  
Sanjiv Karale ◽  
Jagadish V. Kamath
Keyword(s):  
Creatine Kinase ◽  
Histopathological Examination ◽  
Myocardial Damage ◽  
Cardioprotective Effect ◽  
Cardiac Biomarkers ◽  
Oxidative Enzymes ◽  
Histopathological Study ◽  
Marker Enzyme ◽  
Creatine Kinase Isoenzyme ◽  
Mentha Longifolia

<p><strong>Objective: </strong>The current research was designed to evaluate the cardioprotective activity of <em>Mentha longifolia</em> (ML) leaf extract on cyclophosphamide-induced cardiotoxicity in rats.</p><p><strong>Methods: </strong>Cardiotoxicity was induced in <em>Albino wistar</em> rats of either sex by administering a single injection of cyclophosphamide (200 mg/kg, i. p.) on the first day of the experimental period. <em>Mentha longifolia</em> (250 and 500 mg/kg, p. o.) was administered daily for 10 d immediately after administration of cyclophosphamide on the first day. The general observations such as oxidative marker enzyme assays, ECG and histopathology were examined.</p><p><strong>Results: </strong>Cyclophosphamide administration significantly (p&lt;0.05) increased lipid peroxidation (LPO) and decreased the levels of antioxidant markers such as superoxide dismutase (SOD) and catalase (CAT). Cyclophosphamide elevated the levels of biomarker enzymes like creatine kinase isoenzyme MB (CK-MB), creatine kinase isoenzyme NAC (CK-NAC) and lactate dehydrogenase (LDH). Further, the cyclophosphamide-treated rats showed changes in electrocardiographic parameters. Treatment with <em>Mentha longifolia</em> significantly (p&lt;0.05) reversed the status of cardiac biomarkers, ECG and oxidative enzymes in cyclophosphamide-induced cardiotoxicity. Histopathological examination was also supported the potential cardioprotective effect of <em>Mentha longifolia</em> with reduced damage to the myocardium.</p><p><strong>Conclusion: </strong>The biochemical, ECG and histopathology reports support the potential benefits of <em>Mentha longifolia</em> against myocardial damage which could be attributed to antioxidant activity.</p>

scholarly journals Effect of Calcium Carbide on Rat Tissue

1970 ◽  
Vol 6 (2) ◽  
pp. 93-98 ◽  
Author(s):  
Younus Patoare ◽  
Md Iqbal Hossain ◽  
Mohammad Nurul Islam ◽  
Akhtarunnessa Chowdhury ◽  
Seheli Parveen ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Calcium Carbide ◽  
Cell Extract ◽  
Control Group ◽  
Histopathological Study ◽  
Histopathological Analysis ◽  
Whole Cell ◽  
Group I ◽  
Collecting Tubules ◽  
Whole Cell Extract

To evaluate the effects of Calcium carbide (CaC2) in biological system, an in vivo study was carried out on Long Evans rats. CaC2 was administered orally once daily for one month with specific concentrations. Group- I was considered as the control group (without CaC2), Group - II, III, IV and V were the sample groups treated with CaC2 having concentration of 1g/kg, 2g/kg, 5g/kg and 10g/kg body weight respectively. The experiment was conducted to detect any cellular and molecular level changes caused by CaC2. The histopathology and isozyme assay were performed to analyze the changes in the activities of the genes affected by the free radicals released from CaC2. The molecular analysis included different isozymes namely esterase and acid phosphatase. Polyacrylamide electrophoresis of whole cell extract of control subjects and CaC2 administered rats were performed; subsequently the gels were treated with the substrates for acid phosphatase and esterase respectively. No difference was observed in the whole cell extract band pattern between the control subject and the CaC2 administered rats, which grossly indicates that the CaC2 has no effect on the expression pattern of isozymes (acid phosphatase and esterase). Histopathological analysis of liver, heart, spleen, kidney and lungs were performed to observe any change due to the administration of CaC2. Remarkable changes were observed during the histopathological study of lungs and kidney only. The histopathological analysis of kidney showed the thickening of the lining of collecting tubules with changes in cell structure while lungs were found to be increased moderately in weight, with focal areas of consolidation that was found red-brown to red. Key words: Calcium carbide; Histopathological study; Isozymes Dhaka Univ. J. Pharm. Sci. 6(2): 93-98, 2007 (December)

scholarly journals Anti-allergic Activity of 70% Ethanol Extract of Strawberries in Ovalbumin Induced Male Mice

2020 ◽  
Vol 5 (3) ◽  
pp. 92-97
Author(s):  
Siska ◽  
Diene Roufiani ◽  
Ema Dewanti
Keyword(s):  
Ethanol Extract ◽  
Control Group ◽  
Fruit Extract ◽  
Antiallergic Activity ◽  
Group Iii ◽  
Skin Contact ◽  
Group I ◽  
And Control ◽  
Control Group Group

Anaphylaxis is the most common allergic reaction triggered by allergens such as insect poisons, food, and drugs through skin contact, injection, or inhalation. In vitro previous research showed that strawberries fruit have activity as antioxidant, anticancer, anti-inflammation, and anti-allergic. The research aimed to determine the antianaphylaxis strawberry fruit extract in mice (Balb/C strain) with ovalbumin-induced.  Twenty-four Balb/C strain mice were divided into six groups (n=4). Group I and II as a normal and control group. Group III till VI as a treatment group was given cetirizine dose 0.042 mg/20 g BW and strawberry extract doses 0,68; 1,36; and 2,72 mg/20 g BW, respectively. This research showed that 70 % of ethanol extract of strawberries fruit have antiallergic activity in response to active cutaneous anaphylaxis. 70% ethanol extract of strawberries doses 2.72 mg/20 g BW had similar antiallergic activity compare with cetirizine. The conclusion of this study showed that strawberries fruit extract could be developed as an alternative medicine to anti-anaphylaxis or anti-allergic.

scholarly journals HYPOLIPIDEMIC EFFECT OF GINGER EXTRACT IN VANASPATI FED RATS

2013 ◽  
Vol 10 (1-2) ◽  
pp. 93-96 ◽  
Author(s):  
P. Paul ◽  
M. K. Islam ◽  
A. Mustari ◽  
M. Z. I. Khan
Keyword(s):  
Total Cholesterol ◽  
Serum Lipid ◽  
Normal Control Group ◽  
Control Group ◽  
Histopathological Study ◽  
Hypolipidemic Effect ◽  
Simultaneous Administration ◽  
Ginger Extract ◽  
Group Iii ◽  
Group I

The aim of the present study was to evaluate the hypolipidemic effect of ginger in vanaspati fed rats. A total of 18 rats of Long Evans strain weighing 200-250 g were randomly assigned to three groups: Group I, normal control; Group II, 5% vanaspati supplement and Group III, 5% vanaspati + ginger extract (300 mg/ 100ml/ kg b. wt./ day) orally. Serum lipid profile was measured at day 1st and day 49th. The administration of vanaspati augmented the total cholesterol, LDL-C, triglycerides levels and decreased the HDL-C level significantly (p <0.05). Simultaneous administration of ginger extract significantly (p <0.05) prevented the rise in total cholesterol, LDL-C(bad cholesterol), triglycerides levels and rise HDL(good cholesterol). In histopathological study, no significant changes were found in the liver and aorta of all treated groups as compared with control group. It is concluded that ginger extract showed hypolipidemic effect in vanaspati supplemented rats.DOI: http://dx.doi.org/10.3329/bjvm.v10i1-2.15652

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