scholarly journals Deleterious Effects of Storage Environmental Conditions on The Seed Quality of Two Varieties of Tomato (Solanum lycopersicum L.) Stored in Triple Laminated Aluminum Packing in Sri Lanka

2020 ◽  
Vol 8 (4) ◽  
pp. 437-447
Author(s):  
R.A.I.S. Ariyarathna ◽  
S. L. Weerasena ◽  
C. K. Beneragama

Tomato is one of the most commonly growing vegetable crops among the farmers in Sri Lanka. Standard laboratory germination of tomato seeds fulfills the regulatory requirements of seed marketing. However, poor field performance is an overwhelming problem to farmers. Present study was focused on the longevity of two varieties of tomato seeds as affected by their quality characters (percentage germination, moisture, field emergence and vigour index, seed protein and carbohydrate contents) including seed health (exposure of Clavibacter michiganensis subsp. Michiganensis (CMMV), Spotted wilt virus (TSWV) and Leaf curly top virus (LCTV)) under four different storage environmental conditions during a one-year long storage study. Seeds were packed in triple-laminated aluminum packets (TLA) and stored in low temperature storage conditions (17±1 °C and 65% RH) and in ambient conditions in Gannoruwa, Kundasale, and MahaIlluppallama that represent the three agro-ecologically zones; Mid Country Wet Zone (MCWZ), Mid Country Intermediate Zone (MCIZ) and Low Country Dry Zone (LCDZ) respectively. Seed quality parameters displayed varied responses depending on the variety. Physiological changes that occur in seeds due to temperature, RH, changes in proteins and carbohydrate content, CMMV and TSWV during storage were manifested as reduction in seed germination, field emergence and vigour index. However, none of the seedling carried LCTV and therefore its effect on seed quality parameters could not be assessed. The seeds could be stored for ca. 29 months at low and constant temperatures and RH conditions without compromising local seed germination standards compared to ambient storage in the three environments. CMMV and TSWV detected in all seed samples indicated potential threats to tomato farmers in Sri Lanka.  

2020 ◽  
Vol 8 (1) ◽  
pp. 33-44
Author(s):  
R.A.I.S. Ariyarathna ◽  
S.L. Weerasena ◽  
C.K. Beneragama

Bean is a popular vegetable grown in most parts of Sri Lanka. Farmers and home gardeners purchase seeds in packeted form retail outlets. However, quality problems are frequently reported, especially on low field germination. Quality parameters namely, percentage germination, field emergence, seedling length, vigour index and chlorophyll fluorescence analysis were used to determine the effect of different storage environmental conditions on seed longevity of two bean varieties during a two-year storage period, 2016 to 2018. Seeds in triple-laminated packets stored in four contrasting conditions; low temperature storage conditions (Tem. 5oC, 65% RH); three geo-environmental conditions namely, Gannoruwa (mid country wet zone), Kundasale (mid country intermediate zone), and Mahailuppallama (low country dry zone) showed varied behavior in seed quality parameters respective of the variety. It was apparent that germination test alone does not represent the full potential of field emergence. Results of the present study revealed that when field emergence and vigour are also taken in to account, both varieties of bean seeds can be stored under ambient conditions at Gannoruwa, Kundasale or MahaIlluppallama for a maximum period of 12 months, without compromising the seed quality. The seeds stored under low temperature conditions (i.e. 5 °C), maintained the seed germination over 75% for a period of 24 months.  


2018 ◽  
Vol 40 (1) ◽  
Author(s):  
Renato Teo Barros ◽  
Cibele Chalita Martins ◽  
Francisco Elder Carlos Bezerra Pereira ◽  
Givanildo Zildo da Silva

Abstract Seed dormancy may decrease during storage and some environmental conditions may accelerate this process. The aim of this work was to determine efficient techniques to condition umbu seeds in order to promote and standardize their germination. Seeds were stored for 180 days in paper bags kept in five ambient conditions: laboratory (25 ºC and 55% RH); warm oven (40 °C and 53% RH); hot oven (50 °C and 49% RH); dry chamber (18 °C and 65% RH) and cold chamber (10 °C and 65% RH). Seed quality was evaluated every 60 days by means of the following tests and determinations: water content; germination test (25 °C and 55% RH, weekly evaluated up to 91 days after sowing); first count test (14 days); germination rate index; mean germination time and electrical conductivity. The conditioning of umbu seeds in laboratory, or in warm oven (40 ºC) used efficient techniques to promote and standardize germination; under these conditions, after six months of storage, germination increased from 31% to 84 and 74%, respectively.


Author(s):  
S. Kavitha ◽  
P. Srimathi

Background: Black gram is the important pulses crop, grown throughout the country; gives low seed yield mainly due to poor management and low soil fertility. Development of suitable technology is essential to enhance productivity. Seed priming is one such effective technology which enhance rapid and uniform emergence to achieve high vigour, leading to better stand establishment and yield. The current research aimed to study micronutrient seed priming and rhizobium coating on seed germination, seedling vigour, crop growth and seed yield in black gram.Methods: Blackgram seeds were primed with 10 different micronutrient solutions with different concentrations and evaluated for its quality parameters to find out suitable seed priming technique. Seeds from two best priming treatments were coated with rhizobium @ 30g/kg of seed and evaluated for its production potential and resultant seed quality under field condition.Result: Seed priming with 0.5% ZnSO4 at 1/3rd seed to solution ratio for 3 hours recorded higher germination (96%) and vigour index (3811) than control which recorded 84% seed germination and 2528 of vigour index under laboratory experiment. Field experiments revealed that, seeds primed with 0.5% ZnSO4 and coated with rhizobium @ 30g per kg of seeds improved seed yield by 13.2% over control. 


2017 ◽  
Vol 9 (1) ◽  
pp. 482-491
Author(s):  
Umesha Umesha ◽  
B. C. Channakeshava ◽  
K. Bhanuprakash ◽  
D. Nuthan ◽  
R. Siddaraju ◽  
...  

An experiment was conducted to investigate the influence of packaging materials and seed treatments on storability of cluster bean under ambient conditions of Bengaluru. The experiment consisted of six treatments viz. control (T1), bavistin @ 2 g kg-1 (T2), spinosad @ 0.04 ml/kg (T3), neem leaf powder @ 1:20 ratio (T4), Acorus calamus@ 10 g kg-1 (T5) and cow dung powder @ 10 g kg-1 (T6) and three packaging materials viz., cloth bag (C1) super grain bag (C2) and poly lined cloth bag (C3). Treated seed samples were stored in three containers under ambient storage conditions up to the duration of which seeds maintain minimum seed certification standards and samples were drawn at bimonthly intervals for ascertaining the seed quality parameters. The study suggested that seed treat-ment could be useful to prolong the storage life of cluster bean seeds. The seeds treated with spinosad (0.04 ml/kg) and stored in super-grain bag were better for maintenance of higher seed quality parameters [germination (80.00%), root length (11.70 cm), shoot length (13.60 cm), mean seedling dry weight (152 mg), seedling vigour index I and II (2024&12140) and TDH activity (1.224) with low electrical conductivity (0.368 dSm-1)] up to 18 months under ambient conditions of Bengaluru (room temperature). Super-grain bag proved to be better storage container with higher seed quality attributes viz., germination (72.38 %), seedling vigour index-I (1726), total dehydrogenase activity (1.201) and other seed quality parameters compared to cloth bag. The study suggested that use of appropriate packaging material and seed treatment could be useful to prolong the storage life of cluster bean seeds.


Author(s):  
Pramod Sharma ◽  
Arun Bhatt ◽  
Rajesh Kanwar

The present investigation was carried out in the Department of Seed Science and Technology, College of Forestry, Ranichauri, Tehri Garhwal, Uttarakhand during 2015. A laboratory experiment was conducted to find out the influence of bio-priming on seed germination and seedling vigour. Seed germination was reported maximum for Psf-173 (93.66%) while minimum was reported for control (65.66%). Seed vigour parameter viz. root length (11.01 cm), shoot length (7.96 cm), seedling length (18.98 cm), fresh weight (8.00 g) and dry weight (1.31 g), vigour index I (1666.80) and vigour index II (115.56) was found higher for PSB treatment. Shilajeet variety had recorded significant higher values for most of the germination and vigour parameters. Shilajeet bio-primed with PSB attained higher values for majority of the quality parameters. Under laboratory condition results suggested that seed germination and seed vigour were greatly affected by different bio-agents. Among all the bio-agents, PSB showed positive influence on most of the quality parameter. However, other bio-agents had also showed significant effect over control.


Author(s):  
B. Vaidya P. B. Sable ◽  
V. B. Patil

The study revealed that China aster seeds lose viability quickly if stored under ambient conditions. At the end of 12th months, seeds stored at refrigerated condition (5-70C) still maintained high seed germination (64.00%) at field condition well above the minimum seed certification standards. However, in case of ambient storage very drastic reduction in seed germination was observed. It was 32.43 % for first months and reduced to 14.95 % for second month by declining 53.90% as compared to first month and thereafter no germination was recorded at field condition. Results from the interaction effect indicated that previously stored seeds of China aster for six months in aluminium foil and kept under refrigerated condition recorded higher seed quality parameters viz. percent seed germination at field (92.67 and 72.00%), percent survival of seedlings (89.33 and 70.67%), height of seedling (11.53 and 8.60 cm), shoot length (7.00 and 5.10 cm), root length (4.53 and 3.50 cm), shoot weight (0.42 and 0.33 g), root weight (0.040 and 0.027 g), root:shoot ratio (0.095 and 0.082) and percent seed germination at laboratory (97.33 and 76.00%) for first and sixth month, respectively.


Author(s):  
S. B. Gawade ◽  
S. R. Zanjare ◽  
A. V. Suryawanshi ◽  
V. R. Shelar

Seeds of mungbean (Cv. Vaibhav) was treated with bioagents and botanicals <italic>viz;</italic> talc powder formulations of <italic>Pseudomonas fluorescens</italic> (0.6%), <italic>Trichoderma viride</italic> (0.6%), <italic>Pseudomonas fluorescens + Trichoderma viride</italic> (0.6%) each, garlic extract (1%), ginger extract (1%) and Thiram + Carbendazim (0.2% each) as a recommended fungicidal check. The results indicated that, among the bioagents the treatment of <italic>Pseudomonas fluorescens + Trichoderma viride</italic> (0.6% each) to the seeds of mungbean was effective in reducing seed mycoflora <italic>viz; Fusarium oxysporum, Alternaria alternata, Macrophomina phaseolena</italic>, <italic>Aspergillus flavus, Aspergillus niger</italic> and <italic>Fusarium moniliforme</italic> by 88.5, 85.1, 64.9, 81.1, 75.0 and 100 per cent, respectively over untreated control. The seed germination, seedling vigour index and field emergence of mungbean was increased by 21.81, 34.03 and 15.98 per cent, respectively with this treatment over untreated control. The seed germination, seedling vigour index and field emergence in untreated control was 71.83 per cent, 2261.52 and 67.83 per cent, respectively.


Author(s):  
Shivasharanappa . ◽  
S. Patil ◽  
S. R. Doddagoudar ◽  
Vijay Kumar Kurnalliker ◽  
Rakesh . ◽  
...  

An attempt was made to predict the relative storability of seeds of soybean varieties (JS-335 and DSB-21) through accelerated ageing technique in comparison with that of natural ageing where both the varieties had similar initial germination (93 %). The seeds were subjected to accelerated ageing conditions at 41 oC temperature and 95 ± 5 per cent relative humidity up to 180 hours. While, for natural ageing, the seeds were packed in cloth bag with the initial moisture of 9.5 per cent and stored under ambient conditions. The experimental results revealed that with the subsequent increase in accelerated ageing duration from 24 hours onwards up to 180 hours, the seed quality parameters viz., seed germination percentage, mean germination time, seedling vigour index and dehydrogenase enzyme activity decreased significantly. While the electrical conductivity increased significantly. When the accelerated ageing results were compared with that of natural ageing, accelerated ageing for 132 hours (67 % germination) was nearly equal to nine months of natural ageing (69 % germination) in case of JS-335 whereas in DSB-21, 144 hours of accelerated ageing (70 % germination) was equal to nine months of natural ageing (70 % germination). Hence, the accelerated ageing test can be used to predict the relative storability of soybean seeds. Hence, the seed producers can take decision regarding which seed lot can be stored for longer time and which one should be immediately sold to the market without any compromise in seed quality. It also helps in taking other management decisions.


2016 ◽  
Vol 8 (3) ◽  
pp. 1638-1642
Author(s):  
Gajendra Khidrapure ◽  
S. N. Vasudevan ◽  
S. R. Doddagoudar ◽  
A. G. Sreenivas ◽  
Satyanarayana Rao

The present study was conducted to know the storage potential of organically produced paddy seeds in the Department of Seed Science and Technology, University of Agricultural Sciences, Raichur. The seed lot were divided into two parts, one part was stored in cloth bag for a period of 12 months under ambient conditions. At thesame time another set of seeds were subjected to accelerated ageing at 42 + 10C temperature and 90 per cent relative humidity (RH) for a period of 0-12 days. Among the ageing methods, artificially aged seeds showed drastic decreases in seed quality as compared to natural ageing. Among the treatments T9 (37.5 % FYM + 37.5 % vermicompost + 25 % neem cake + foliar spray of panchagavya on 30, 60, 90 and 120 DAT) recorded significantly highest seed quality parameters viz., seed germination (97.81 %), seedling length (29.42 cm) and SVI (2878) at initial stage in both the method of aging and at the end of storage period; seed germination (71.23 and 87.33 %), seedling length (19.66 and 27.00 cm) and SVI (1400 and 2358) in accelerated ageing (AA) and natural ageing (NA) respectively, whereas, lowest in control (Inorganic treatment). The seed quality parameters of four days of AA were similar to that of six months of NA. Hence, storability of organically produced paddy seeds were better as compared to inorganic seeds and it can be predicted that four days of AA is equal to six months of NA. The information generated will be useful in retention or disposal of a particular variety or seed lot.


Author(s):  
N. Aiswarya ◽  
S.L. Bhattiprolu ◽  
K. Bayyapu Reddy ◽  
G. Rama Rao

Background: Aspergillus niger and A. flavus cause rotting, damping-off, loss in germination and toxification of oil seeds. Aspergillus is a common mold in tropical and Sub-tropical countries and causes aflatoxin contamination. A. flavus and A. niger were responsible for storage rot of groundnut. In addition to storage rot, aflatoxin contamination also leads to adverse effects on consumer health. Therefore, present study was conducted to estimate the impact of both A. niger and A. flavus on seed quality parameters of groundnut. Methods: Effect of major seed borne fungi A.niger and A. flavus on seed quality characters of thirteen different genotypes of groundnut was observed in the present study. All the seedling quality characters were measured as per ISTA guidelines. Result: On an average A. niger caused 25.14, 25.18, 43.99, 26.20 and 44.75 per cent reduction in seed germination, seedling length, seedling vigour index I, seedling dry weight and seedling vigour index II, respectively, over the control whereas A. flavus caused average per cent reduction of 22.38, 24.23, 41.18, 23.92 and 40.95 in seed germination, seedling length, seedling vigour index I, seedling dry weight and seedling vigour index II, respectively, over the control.


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