Effect of Storage Conditions on Seed Viability of China Aster [Callistephus chinensis (L.) Nees]

The study revealed that China aster seeds lose viability quickly if stored under ambient conditions. At the end of 12th months, seeds stored at refrigerated condition (5-70C) still maintained high seed germination (64.00%) at field condition well above the minimum seed certification standards. However, in case of ambient storage very drastic reduction in seed germination was observed. It was 32.43 % for first months and reduced to 14.95 % for second month by declining 53.90% as compared to first month and thereafter no germination was recorded at field condition. Results from the interaction effect indicated that previously stored seeds of China aster for six months in aluminium foil and kept under refrigerated condition recorded higher seed quality parameters viz. percent seed germination at field (92.67 and 72.00%), percent survival of seedlings (89.33 and 70.67%), height of seedling (11.53 and 8.60 cm), shoot length (7.00 and 5.10 cm), root length (4.53 and 3.50 cm), shoot weight (0.42 and 0.33 g), root weight (0.040 and 0.027 g), root:shoot ratio (0.095 and 0.082) and percent seed germination at laboratory (97.33 and 76.00%) for first and sixth month, respectively.

Download Full-text

Effect of Seed Treatments and Containers on Chilli and Brinjal Seed Viability

Current Journal of Applied Science and Technology ◽

10.9734/cjast/2019/v36i230221 ◽

2019 ◽

pp. 1-9

Author(s):

Sunil Kumar ◽

Suryapal Singh ◽

S. S. Jakhar

Keyword(s):

Electrical Conductivity ◽

Seed Treatment ◽

Seed Quality ◽

Seed Viability ◽

Quality Parameters ◽

Packaging Material ◽

Packaging Materials ◽

Ambient Conditions ◽

Pathology Laboratory ◽

Seed Pathology

An experiment was conducted to investigate the influence of fungicides and packaging materials on longevity of chilli (variety: RCH-1) and brinjal (variety: Hisar Shyamal) seeds. The seeds were treated with 15 fungicides and were kept in three containers viz. Metal box, Cloth bag and Plastic zipling bag up to 12 months under ambient conditions in seed pathology laboratory of Department of Seed Science & Technology, CCSHAU, Hisar, India. The samples were drawn at quarterly intervals for ascertaining the seed quality parameters. The seeds treated with flusilazole and carbendazim (2 g kg-1 seed) in case of chilli and brinjal and stored in metal-box were found better for maintenance of higher seed quality parameters [electrical conductivity and dehydrogenase activity] during the study period. The study suggested that use of appropriate packaging material and seed treatment could be useful to prolong the storage life of chilli and brinjal seeds.

Download Full-text

Quality assessment of Urochloa (syn. Brachiaria) seeds produced in Cameroon

Scientific Reports ◽

10.1038/s41598-021-94246-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Njehoya Clémence-Aggy ◽

Ntchapda Fidèle ◽

Kana Jean Raphael ◽

Etchu Kingsley Agbor ◽

Sita R. Ghimire

Keyword(s):

Seed Germination ◽

Quality Assessment ◽

Seed Quality ◽

Seed Viability ◽

Quality Parameters ◽

North West ◽

Grass Seed ◽

Significant Relationships ◽

Livestock Farmers

AbstractUrochloa (syn. Brachiaria) is the most popular fodder of livestock farmers in Cameroon for hay and seed productions. Farmers in Cameroon have been producing Brachiaria seeds for decades for own uses and surplus are sold to neighbours, and to traders from Cameroon and neighbouring countries. However, there is no information available about qualities of these seeds. Fifteen Urochloa seeds samples were collected from farmers and/or government stations in five regions (Adamaoua, East, North, North West, and West) and analysed for major seed quality parameters along with seeds of improved Urochloa cultivar Basilisk imported from Brazil as a check. Study showed significant differences among treatments for various seed quality parameters tested (P < 0.0001). The highest thousand grains weight was recorded in Basilisk (5.685 g), followed by W12 (3.555 g), A05 (3.153 g) and N01 (2.655 g). Caryopsis number and caryopsis weight were highest in Basilisk followed by E09, A06, and W12. Of three conditions tested for seed germination, mean germination was the highest in greenhouse (7.39%) where Basilisk had the highest germination (25.5%) followed by N01 (18.50%), A05 (14.50%) and W12 (12.75%). The seed viability ranged from 18% (E09) to 81% (N01), and there were a positive and highly significant relationships between seed germination and viability traits (r = 0.883; P < 0.0001). This study showed a marked difference in seed quality parameters of Urochloa grass seeds produced in Cameroon, and the potential of developing Urochloa grass seed business in the Northern, Adamaoua and Western regions of Cameroon.

Download Full-text

Assessment of Natural Ageing Related Physio-Biochemical Changes in Onion Seed

Agriculture ◽

10.3390/agriculture9080163 ◽

2019 ◽

Vol 9 (8) ◽

pp. 163 ◽

Cited By ~ 3

Author(s):

Brar ◽

Kaushik ◽

Dudi

Keyword(s):

Seedling Establishment ◽

Seed Quality ◽

Seed Viability ◽

Seed Storage ◽

Germination Percentage ◽

Quality Parameters ◽

Biochemical Changes ◽

Ambient Conditions ◽

Onion Seed ◽

One Year

This research was performed for a period of three years to determine the effect of natural seed ageing on different physiological and biochemical changes in three extensively cultivated onion cultivars in Northern India. Seed storage studies showed that germination percent, seed viability and other seed quality parameters decreased significantly with increasing storage time. The onion seed can be stored for a maximum period of one year under ambient conditions to maintain required germination percentage (>70%). Overall, Hisar Onion-4 stored better when compared to Hisar Onion-3 and Hisar-2. As the ageing progressed the seed antioxidants, namely superoxide dismutase (SOD), catalase (CAT), dehydrogenase (DHA) and peroxidase (POD) decreased significantly (p < 0.05) in all cultivars whereas, the electrical conductivity (EC) of seed leachates increased significantly. Under the field conditions, seedling establishment percent (SE) onion seed was positively correlated (R2 = 0.98; p < 0.05) with germination percent (GP). Overall, more than one year period of seed storage was associated with poor germination and seedling establishment potential in onion.

Download Full-text

Effect of Seed Treatments and Containers on Chilli Seed Viability

Journal of Experimental Agriculture International ◽

10.9734/jeai/2019/v36i230230 ◽

2019 ◽

pp. 1-10

Author(s):

Sunil Kumar ◽

S. S. Jakhar

Keyword(s):

Seed Treatment ◽

Seed Quality ◽

Seed Viability ◽

Dry Weight ◽

Quality Parameters ◽

Packaging Material ◽

Shoot Length ◽

Ambient Conditions ◽

Pathology Laboratory ◽

Seed Pathology

An experiment was conducted to investigate the influence of fungicides and packaging materials on longevity of chilli seed (variety: RCH-1). The seeds were treated with 15 fungicides and were kept in three containers viz. Metal box, Cloth bag and Plastic zipling bag up to 12 months under ambient conditions in seed pathology laboratory of Department of Seed Science & Technology, CCSHAU, Hisar, India. The samples were drawn at quaterly intervals for ascertaining the seed quality parameters. The seeds treated with flusilazole (2 g kg-1 seed) and stored in metal-box were found better for maintenance of higher seed quality parameters [germination, root length, shoot length, mean seedling dry weight, vigour indices] during the study period. The study suggested that use of appropriate packaging material and seed treatment could be useful to prolong the storage life of chilli seeds.

Download Full-text

Deleterious Effects of Storage Environmental Conditions on The Seed Quality of Two Varieties of Tomato (Solanum lycopersicum L.) Stored in Triple Laminated Aluminum Packing in Sri Lanka

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v8i4.33670 ◽

2020 ◽

Vol 8 (4) ◽

pp. 437-447

Author(s):

R.A.I.S. Ariyarathna ◽

S. L. Weerasena ◽

C. K. Beneragama

Keyword(s):

Sri Lanka ◽

Seed Germination ◽

Environmental Conditions ◽

Seed Quality ◽

Quality Parameters ◽

Vegetable Crops ◽

Ambient Conditions ◽

Tomato Seeds ◽

Vigour Index ◽

Field Emergence

Tomato is one of the most commonly growing vegetable crops among the farmers in Sri Lanka. Standard laboratory germination of tomato seeds fulfills the regulatory requirements of seed marketing. However, poor field performance is an overwhelming problem to farmers. Present study was focused on the longevity of two varieties of tomato seeds as affected by their quality characters (percentage germination, moisture, field emergence and vigour index, seed protein and carbohydrate contents) including seed health (exposure of Clavibacter michiganensis subsp. Michiganensis (CMMV), Spotted wilt virus (TSWV) and Leaf curly top virus (LCTV)) under four different storage environmental conditions during a one-year long storage study. Seeds were packed in triple-laminated aluminum packets (TLA) and stored in low temperature storage conditions (17±1 °C and 65% RH) and in ambient conditions in Gannoruwa, Kundasale, and MahaIlluppallama that represent the three agro-ecologically zones; Mid Country Wet Zone (MCWZ), Mid Country Intermediate Zone (MCIZ) and Low Country Dry Zone (LCDZ) respectively. Seed quality parameters displayed varied responses depending on the variety. Physiological changes that occur in seeds due to temperature, RH, changes in proteins and carbohydrate content, CMMV and TSWV during storage were manifested as reduction in seed germination, field emergence and vigour index. However, none of the seedling carried LCTV and therefore its effect on seed quality parameters could not be assessed. The seeds could be stored for ca. 29 months at low and constant temperatures and RH conditions without compromising local seed germination standards compared to ambient storage in the three environments. CMMV and TSWV detected in all seed samples indicated potential threats to tomato farmers in Sri Lanka.

Download Full-text

Prediction of storability of organically produced paddy seeds through natural and accelerated ageing techniques

Journal of Applied and Natural Science ◽

10.31018/jans.v8i3.1015 ◽

2016 ◽

Vol 8 (3) ◽

pp. 1638-1642

Author(s):

Gajendra Khidrapure ◽

S. N. Vasudevan ◽

S. R. Doddagoudar ◽

A. G. Sreenivas ◽

Satyanarayana Rao

Keyword(s):

Seed Germination ◽

Seed Quality ◽

Foliar Spray ◽

Storage Period ◽

Quality Parameters ◽

Accelerated Ageing ◽

Natural Ageing ◽

Ambient Conditions ◽

Seedling Length ◽

Cent Relative Humidity

The present study was conducted to know the storage potential of organically produced paddy seeds in the Department of Seed Science and Technology, University of Agricultural Sciences, Raichur. The seed lot were divided into two parts, one part was stored in cloth bag for a period of 12 months under ambient conditions. At thesame time another set of seeds were subjected to accelerated ageing at 42 + 10C temperature and 90 per cent relative humidity (RH) for a period of 0-12 days. Among the ageing methods, artificially aged seeds showed drastic decreases in seed quality as compared to natural ageing. Among the treatments T9 (37.5 % FYM + 37.5 % vermicompost + 25 % neem cake + foliar spray of panchagavya on 30, 60, 90 and 120 DAT) recorded significantly highest seed quality parameters viz., seed germination (97.81 %), seedling length (29.42 cm) and SVI (2878) at initial stage in both the method of aging and at the end of storage period; seed germination (71.23 and 87.33 %), seedling length (19.66 and 27.00 cm) and SVI (1400 and 2358) in accelerated ageing (AA) and natural ageing (NA) respectively, whereas, lowest in control (Inorganic treatment). The seed quality parameters of four days of AA were similar to that of six months of NA. Hence, storability of organically produced paddy seeds were better as compared to inorganic seeds and it can be predicted that four days of AA is equal to six months of NA. The information generated will be useful in retention or disposal of a particular variety or seed lot.

Download Full-text

Seed longevity of maize conserved under germplasm bank conditions for up to 60 years

Annals of Botany ◽

10.1093/aob/mcab009 ◽

2021 ◽

Author(s):

Filippo Guzzon ◽

Maraeva Gianella ◽

Jose Alejandro Velazquez Juarez ◽

Cesar Sanchez Cano ◽

Denise E Costich

Keyword(s):

Seed Germination ◽

Plant Genetic Resources ◽

Seed Viability ◽

Storage Conditions ◽

Seed Longevity ◽

Germplasm Bank ◽

Significant Difference ◽

Different Temperatures ◽

Germination Experiments

Abstract Background and Aims The long-term conservation of seeds of plant genetic resources is of key importance for food security and preservation of agrobiodiversity. Nevertheless, there is scarce information available about seed longevity of many crops under germplasm bank conditions. Methods Through germination experiments as well as the analysis of historical monitoring data, we studied the decline in viability manifested by 1000 maize (Zea mays subsp. mays) seed accessions conserved for an average of 48 years at the CIMMYT germplasm bank, the largest maize seedbank in the world, under two cold storage conditions: an active (–3 °C; intended for seed distribution) and a base conservation chamber (–15 °C; for long-term conservation). Key Results Seed lots stored in the active chamber had a significantly lower and more variable seed germination, averaging 81.4 %, as compared with the seed lots conserved in the base chamber, averaging 92.1 %. The average seed viability detected in this study was higher in comparison with that found in other seed longevity studies on maize conserved under similar conditions. A significant difference was detected in seed germination and longevity estimates (e.g. p85 and p50) among accessions. Correlating seed longevity with seed traits and passport data, grain type showed the strongest correlation, with flint varieties being longer lived than floury and dent types. Conclusions The more rapid loss of seed viability detected in the active chamber suggests that the seed conservation approach, based on the storage of the same seed accessions in two chambers with different temperatures, might be counterproductive for overall long-term conservation and that base conditions should be applied in both. The significant differences detected in seed longevity among accessions underscores that different viability monitoring and regeneration intervals should be applied to groups of accessions showing different longevity profiles.

Download Full-text

Impacts of different salt source and concentrations on germination and seedling growth of many pumpkin seeds used as rootstoch in Iran

Genetika ◽

10.2298/gensr1202235d ◽

2012 ◽

Vol 44 (2) ◽

pp. 235-250 ◽

Cited By ~ 1

Author(s):

Ahmad Dadashpour

Keyword(s):

Electrical Conductivity ◽

Seed Germination ◽

Seedling Growth ◽

Root Length ◽

Germination Rate ◽

Shoot Length ◽

Root Weight ◽

Pumpkin Seeds ◽

High Concentrations ◽

Shoot Weight

The effects of different salt sources (C Cl2, NaCl, and KCl) and concentrations, as measured by electrical conductivity, (0, "control", 1, 3, 5, 7 and 9 dS m-1) on seed germination and seedling growth of ?Ferro?, ?Obez?, ?RS 841? and ?Strong Tosa F1? pumpkin varieties used as rootstock were investigated in this study. The results showed that germination rate, root length, shoot length, fresh root weight, dry root weight, fresh shoot weight and dry shoot weights tend to decrease when the electrical conductivity of the solution is higher than 5 dS m-1, independent of salt sources and in all of the varieties. Three days after seeding, a germination ratio of 5 % was obtained from RS 841 variety in all salt source and concentrations, while a germination ratio over 50 % was obtained in ?Strong Tosa? variety for the same conditions except CaCl2 salt source. Nevertheless, seeds germinated in medium having high concentrations of CaCl2 had lower germination rate and poor seedling growth, compared to media having the same concentrations of NaCl and KCl. It was concluded that all of the varieties studied were more sensitive to the concentrations prepared using CaCl2 than that of the KCl, and NaCl.

Download Full-text

Optimization and sensitivity analysis of fast ethanol assay in maize seeds

Journal of Seed Science ◽

10.1590/2317-1545v41n1206316 ◽

2019 ◽

Vol 41 (1) ◽

pp. 97-107 ◽

Cited By ~ 1

Author(s):

Damrongvudhi Onwimol ◽

Thunyapuk Rongsangchaicharean ◽

Pitipong Thobunluepop ◽

Tanapon Chaisan ◽

Wanchai Chanprasert

Keyword(s):

Quality Assessment ◽

Ethanol Production ◽

Seed Quality ◽

Storage Conditions ◽

Ambient Conditions ◽

Maize Seed ◽

Seed Deterioration ◽

Maize Seeds ◽

Hermetic Seal

Abstract: The evaluation of seed deterioration is very important to control the quality of the seeds stored. This study aimed to investigate the potential of fast ethanol assay for seed quality assessment of maize stored under different conditions. The first experiment was to determine the incubating temperature, incubating time, and amount of seed used in the assay. The results showed that the best protocol for the detection of headspace ethanol was incubation of 3 g of maize seed with 20% moisture content (wet basis) in a 20 mL gas chromatography vial at 70 °C for 1.5 h. The assay induced approximately 200-700 µg.L-1 of headspace ethanol, which was sufficient to identify seeds with different vigour levels. In the second experiment, the optimal conditions were used for quality assessment in aged maize seed stored for 12 months under different storage conditions. The increase in the ethanol production of stored maize seed under the controlled conditions (15 °C and 20% RH in the hermetic seal) was lower than under ambient conditions. The ethanol production levels of maize seed samples at the start of storage was significantly lesser than at six months storage (p < 0.05). The test limitations in deteriorated seed with different cultivars and ages will be discussed.

Download Full-text

Physiology of Oil Seeds. X. Seed Quality of Peanut Genotypes as Affected by Ambient Storage Temperature1

Peanut Science ◽

10.3146/i0095-3679-19-2-3 ◽

1992 ◽

Vol 19 (2) ◽

pp. 72-77 ◽

Cited By ~ 7

Author(s):

Darold L. Ketring

Keyword(s):

Ambient Temperature ◽

Seedling Growth ◽

Seed Quality ◽

Storage Temperature ◽

Storage Conditions ◽

Ambient Conditions ◽

Ambient Storage ◽

Wide Range ◽

Genotype Diversity ◽

Field Emergence

Abstract Proper storage of peanut (Arachis hypogaea L.) pods following drying is a critical step in maintenance of seed quality for the next planting season. The objective of this study was to examine the effect of ambient storage temperature (similar to farmers stock peanuts stored in warehouses) on seed germination and seedling vigor of selected peanut genotypes. Peanuts were grown in the field for three successive seasons. Pods were harvested, dried, and shelled. Seed samples were taken for storage under ambient conditions, humidity effects were eliminated by storing the seeds in plastic freezer bags in sealed containers. Seasons were: (1) 1986, 19 months storage at ambient temperature, (2) 1987, 7 months storage at ambient temperature, and (3) 1988, seeds without storage. When comparisons were made among genotypes within seasons, exposure to Season 1 conditions resulted in the least germination and seedling growth, but a wide range of genotype diversity occurred. Germination of seeds from Seasons 2 and 3 ranged from 81 to 98%, and significant differences in seedling growth occurred among genotypes. When comparisons were made across Seasons, the data indicated a significant storage effect, which resulted in different seed quality for individual genotypes. Usually field emergence was significantly different among genotypes and was highly correlated with germination for all seasons. Generally, emergence was negatively correlated with slow seedling growth and positively correlated with rapid seedling growth. Thus, for seeds of lower vigor (Seasons 1 and 2), rapid seedling growth was particularly critical for early, uniform emergence (10 DAP) in the field. Genotypes were significantly different in extent of seed quality reduction and field emergence both within and across storage periods. Genotype diversity to ambient storage conditions suggests there is genetic potential to improve longevity of seed quality during storage and enhance stability of field emergence.

Download Full-text