scholarly journals Isolation and Biochemical Characterization of Indole-3-Acetic Acid (IAA) Produced in Pseudomonas Sp. Isolated from Rhizospheric Soil

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Utkalendu Suvendusekhar Samantaray ◽  
Swapnarani Sethi
Keyword(s):  
Acetic Acid ◽  
Indole Acetic Acid ◽  
Biochemical Characterization ◽  
Stem Elongation ◽  
Lateral Roots ◽  
Rhizospheric Soil ◽  
Bacterial Strains ◽  
Physiological Processes ◽  
Crucial Component ◽  
Indole 3 Acetic Acid

A phytohormone is a molecule that controls the development of plants. Auxin and cytokines, which may be acquired as synthetic compounds or produced by microorganisms, which promote plant growth, are employed to enhance crop yield. Indole-3-Acetic Acid (IAA) is a key phytohormone that controls a number of physiological processes in plants. Indole-3-acetic acid (IAA), the most prevalent endogenous auxin, is involved in stem elongation and root formation. Auxin levels are usually higher in the rhizosphere. The possibility of rhizosphere bacteria to encourage direct plant development has been recognised as a crucial component of auxin (IAA) production. They promote the growth of lateral roots, which increases the surface area available for nutrition absorption and enhances nutrient and water absorption from the soil. The objective of this research is to isolate, characterise, and identify bacteria that produce indole acetic acid in rhizospheric soil. Bacterial colonies were isolated using the serial dillution technique on nutrient agar medium from rhizospheric soil samples of a rice crop. Five rhizospheric bacterial isolates (RIPB-1 to RIPB-30) were identified as effective Indole acetic acid makers after qualitative screening. The quantity of Indole acetic acid produced by five bacterial strains was tested for up to 120 hours. The amount of Indole acetic acid they could generate ranged from 1 to 11.2 g/ml. The bacterial strain RIPB-20 (11.2 g/ml) generated the highest Indole acetic acid after 72 hours of incubation, followed by RIPB-14. Based on morphological, physiological, and biochemical features, the bacterial isolate RIPB-20 was tentatively identified as Bacillus sp. Finally, the finding shows that the bacteria, which have capacity to produce Indole acetic acid, are good biofertilizer inoculants for growth of plant.

Isolation and identification of IAA producing endosymbiotic bacteria from Gracillaria corticata (J. Agardh)

2016 ◽  
Vol 5 (12) ◽  
pp. 5179
Author(s):  
Ilahi Shaik* ◽  
P. Janakiram ◽  
Sujatha L. ◽  
Sushma Chandra
Keyword(s):  
Acetic Acid ◽  
Culture Filtrate ◽  
Indole Acetic Acid ◽  
Shoot Growth ◽  
High Salinity ◽  
Saline Soils ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Endosymbiotic Bacteria ◽  
Root And Shoot Growth

Indole acetic acid is a natural phytohormone which influence the root and shoot growth of the plants. Six (GM1-GM6) endosymbiotic bacteria are isolated from Gracilaria corticata and screened for the production of IAA out of six, three bacterial strains GM3, GM5 and GM6 produced significant amount of IAA 102.4 µg/ml 89.40 µg/ml 109.43 µg/ml respectively. Presence of IAA in culture filtrate of the above strains is further analyzed and confirmed by TLC. As these bacterial strains, able to tolerate the high salinity these can be effectively used as PGR to increase the crop yield in saline soils.

The involvement of indole-3-acetic acid in the control of stem elongation in dark- and light-grown pea (Pisum sativum) seedlings

2008 ◽  
Vol 165 (5) ◽  
pp. 482-489 ◽  
Author(s):  
Carlo Sorce ◽  
Piero Picciarelli ◽  
Gianni Calistri ◽  
Bartolomeo Lercari ◽  
Nello Ceccarelli
Keyword(s):  
Acetic Acid ◽  
Pisum Sativum ◽  
Stem Elongation ◽  
Indole 3 Acetic Acid

scholarly journals Characterization and application of nitrogen-fixing and indole-3-acetic acid producing bacteria A13 in Oil Palm (Elaeisguineensis Jacq.) seedling

2021 ◽  
Vol 3 (1) ◽  
pp. 32-40
Author(s):  
Ismi Isti'anah ◽  
Nisa Mubarik Rachmania ◽  
Aris Tjahjoleksono
Keyword(s):  
Acetic Acid ◽  
Oil Palm ◽  
Lateral Roots ◽  
Rrna Gene ◽  
Good Prospect ◽  
Nitrogen Fixing ◽  
Semisolid Medium ◽  
Acid Producing Bacteria ◽  
Biological Fertilizer ◽  
Indole 3 Acetic Acid

Oil palm plantations have a good prospect in Indonesia. One of the efforts to improve the productivity of oil palm plantation is the application of bacteria as biological fertilizer. The research was conducted to characterize and apply the nitrogen-fixing and indole-3-acetic acid producing bacteria in oil palm seedlings. The bacteria was isolated from soil samples which taken from Taman Nasional Bukit Dua Belas (TNBD) Jambi. Nitrogen free bromthymol blue (NFB) is used as media for nitrogen-fixing bacterial isolation. Selected isolate named A13 had an ability to form white pellicle on the surface of the semisolid medium, increased the pH, and changed the color of medium from green to blue Isolate A13 was identified as Gram-negative bacteria and had a rods shape. Analysis of 16S rRNA gene sequence showed that isolate A13 had a similarity with Pseudochrobactrum assacharolyticum. Hypersensitivity assay on tobacco leaves showed that isolate A13 was not a pathogen. During 48 hours of incubation, isolate A13 produced a maximum of IAA at the 24th hour of incubation. Isolate A13 produced 0.675 ppm of ethylene/hour in Acetylene Reduction Assay and 69,839 ppm of IAA in HPLC methods. This was the first report on nitrogen fixation and IAA production by Pseudochrobactrum assacharolyticum and its application in the soil of oil palm seedlings. Application of isolate A13 in oil palm seedling increased significantly the number of lateral roots, stem diameter, and height of plants

scholarly journals Screening of Indole-3-Acetic Acid (IAA) Productions by Endophytic Fusarium oxysporum Isolated from Phyllanthus niruri

1970 ◽  
Vol 7 (1) ◽  
pp. 56-59
Author(s):  
Abdul Raffi Junaidi ◽  
Mohamad Hasnul Bolhassan
Keyword(s):  
Acetic Acid ◽  
Fusarium Oxysporum ◽  
Endophytic Fungi ◽  
Acid Production ◽  
Indole Acetic Acid ◽  
Phyllanthus Niruri ◽  
Acetic Acid Production ◽  
Indole Acetic Acid Production ◽  
Indole 3 Acetic Acid

Ten newly isolated endophytic fungi from Phyllathus niruri Linn. were identified as Fusarium oxysporum. These isolates were screened out for their productivity of indole acetic acid (IAA) by the salkowski's method. Out of these isolates, two isolates showed high amount of indole acetic acid production, which were FO9 and FO10 with concentration of 23.52 μg/ml and 5.95 μg/ml, respectively.

scholarly journals VEGETATIVE PROPAGATION OF Spondias tuberosa E Spondias dulcis WITH THE USE OF IMMERSION IN INDOLE ACETIC ACID

2019 ◽  
Vol 32 (4) ◽  
pp. 858-866
Author(s):  
GABRIELA TEODORO ROCHA ◽  
AYURE GOMES DA SILVA ◽  
JULIANA BEZERRA MARTINS ◽  
NEI PEIXOTO ◽  
FABRICIO RODRIGUES
Keyword(s):  
Acetic Acid ◽  
Vegetative Propagation ◽  
Indole Acetic Acid ◽  
Stem Cuttings ◽  
Randomized Design ◽  
Spondias Tuberosa ◽  
Completely Randomized Design ◽  
Low Efficiency ◽  
Indole 3 Acetic Acid ◽  
Total Fresh Weight

ABSTRACT The objective of this work was to evaluate the effect of six indole-3-acetic acid (IAA) concentrations and three immersion times in IAA solutions on the vegetative propagation of Spondias dulcis and Spondias tuberosa by stem cuttings. The experiment was conducted in a greenhouse, using a completely randomized design, with a 6×3 factorial arrangement consisting of six indole-3-acetic acid concentrations (0, 2, 4, 6, 8, and 10 g L-1) and three immersion times (8, 16, and 24 seconds), with ten cuttings of S. dulcis and S. tuberosa per plot, and three replications. Percentage of surviving cuttings, and percentage of rooted cuttings, number of leaf buds, number of roots per cutting, root length, and total fresh weight were evaluated at 180 days after planting. Data were subjected to analysis of variance by the F test and to regression analysis. Vegetative propagation from woody cuttings of S. dulcis is not viable to produce seedlings, thus, new studies on this technique for this species are needed. Vegetative propagation from woody cuttings of S. tuberosa is satisfactory, but with low efficiency for improvement and emergence of new shoots and roots; it is more effective when using a concentration of 10 g L-1 of indole-3-acetic acid and immersion time in the solution of 16 seconds.

scholarly journals Indole-3-Acetic Acid Is Synthesized by the Endophyte Cyanodermella asteris via a Tryptophan-Dependent and -Independent Way and Mediates the Interaction with a Non-Host Plant

2021 ◽  
Vol 22 (5) ◽  
pp. 2651
Author(s):  
Linda Jahn ◽  
Uta Hofmann ◽  
Jutta Ludwig-Müller
Keyword(s):  
Acetic Acid ◽  
Plant Hormone ◽  
De Novo ◽  
Lateral Roots ◽  
Fungal Endophytes ◽  
Biosynthesis Pathway ◽  
Genome Information ◽  
Indole 3 Acetic Acid ◽  
Iaa Biosynthesis

The plant hormone indole-3-acetic acid (IAA) is one of the main signals playing a role in the communication between host and endophytes. Endophytes can synthesize IAA de novo to influence the IAA homeostasis in plants. Although much is known about IAA biosynthesis in microorganisms, there is still less known about the pathway by which IAA is synthesized in fungal endophytes. The aim of this study is to examine a possible IAA biosynthesis pathway in Cyanodermella asteris. In vitro cultures of C. asteris were incubated with the IAA precursors tryptophan (Trp) and indole, as well as possible intermediates, and they were additionally treated with IAA biosynthesis inhibitors (2-mercaptobenzimidazole and yucasin DF) to elucidate possible IAA biosynthesis pathways. It was shown that (a) C. asteris synthesized IAA without adding precursors; (b) indole-3-acetonitrile (IAN), indole-3-acetamide (IAM), and indole-3-acetaldehyde (IAD) increased IAA biosynthesis; and (c) C. asteris synthesized IAA also by a Trp-independent pathway. Together with the genome information of C. asteris, the possible IAA biosynthesis pathways found can improve the understanding of IAA biosynthesis in fungal endophytes. The uptake of fungal IAA into Arabidopsis thaliana is necessary for the induction of lateral roots and other fungus-related growth phenotypes, since the application of the influx inhibitor 2-naphthoxyacetic acid (NOA) but not the efflux inhibitor N-1-naphtylphthalamic acid (NPA) were altering these parameters. In addition, the root phenotype of the mutation in an influx carrier, aux1, was partially rescued by C. asteris.

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