scholarly journals ANALYSIS OF COMPLEX GENOTYPES OF BETA- AND KAPPA CASEIN OF BULLS OF DAIRY BREEDS

2020 ◽  
Vol 60 ◽  
pp. 99-109
Author(s):  
V. I. Ladyka ◽  
Y. I. Sklyarenko ◽  
Y. M. Pavlenko
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Milk Protein ◽  
Raw Materials ◽  
Milk Proteins ◽  
Breeding Stock ◽  
Breeding Parameters ◽  
Holstein Breed ◽  
Beta Casein ◽  
Main Component

Marker-dependent breeding is one of the most relevant areas for improving the dairy productivity of cattle. It allows the selection of parent forms at the gene level. The growing demands of the market for milk quality make it necessary to select dairy herds using genetic markers associated with qualitative characteristics of milk productivity. A topical issue in dairy cattle breeding today is the study of the relationship between hereditary factors that determine the types of proteins in milk. Casein is the main component of milk proteins and is represented by three fractions – alpha (CSN1S1), beta (CSN2) and kappa (CSN3). The content of individual casein fractions depends on the breed of cows. Genetic variants of beta-casein significantly affect human health, while kappa-casein is associated with the quality of milk and the greater suitability of milk for processing and cheese production. The aim of this work is to evaluate the genotype of sires approved for use in Ukrainian farms in 2020 based on beta- and kappa-casein genes. Materials and methods. The research was conducted by analyzing the catalog of bulls of dairy and dairy-meat breeds for breeding stock reproduction in 2020. Bulls were analyzed according to the genotype of beta – and kappa-casein of Holstein (n = 872), Simmental (n = 17), Montbellard (n = 3), (wro.synel.net; motbeliarde.org) Jersey (n = 59), Red Danish (n = 8), Red Norwegian (n = 7), Schwyz (n = 17), Aishir (n = 8) breeds, evaluated by offspring quality and genomic assessment. The website DairyBulls.com is used to analyze the results of the evaluation of bulls-producers on a set of characteristics. The research results were processed by mathematical statistics methods using the "Statistica-6.1" package in a Windows PC environment. It was found that the majority of bulls-producers approved for use were evaluated based on these genotypes. Depending on the breed, the number of bulls estimated by the studied characteristics ranged from 43 to 85%. Depending on the breed, from 2 to 16 different combinations of beta- and kappa-casein genotypes were identified. The frequency of occurrence of the desired complex genotype A2A2/BB, depending on the breed, was in the range of 0.0 to 75.0%. Holstein bulls of the desired A2A2/BB genotype were estimated to be superior to bulls of some other complex genotypes in terms of daughter milk yield, the amount of milk fat, milk protein, lifetime profit indices, cheese profit, and milk profit. Therefore, the use of sperm production of bulls with the A2A2/BB genotype will improve the economically useful characteristics of offspring, and will further increase the number of livestock with the desired complex genotype. Summary. 1. Taking into account the data of many domestic and foreign scientists who claim that the genotype of animals by the beta – and kappa-casein polymorphism affects the qualitative composition of milk, it is necessary to have in herds the necessary number of animals carrying the A2 allele of beta-casein and the B allele of the kappa-casein gene. The desired breeding parameters of the herd can be achieved by using bulls with BB genotypes for kappa-casein and A2A2 for beta-casein. Correction of the bulls-producers selection system can only be carried out if the breeding stock of the herd is genotyped according to the polymorphism of the beta-and kappa – casein genotypes. Holding this event will increase the protein content and will improve cheese suitability of milk of cattle of future generations. The use of bulls-producers without taking into account their genotypes for the casein fractions described above and the lack of polymorphism control of these genes among cows can lead to a decrease in the frequency of desired genotypes in the herd and a decrease in the technological qualities of milk as raw materials. 2. The created database of bulls-producers approved for use in Ukraine, evaluated by the beta-and kappa – casein genotypes, allows us to establish a positive perspective on the possibility of forming staffed herds of animals with the desired complex A2A2/BB genotype in Ukraine. 3. It was found that bulls of dairy breeds have different frequencies of beta – and kappa casein alleles. The part of bulls-producers of the desired complex genotype A2A2/BB of Holstein breed is 8.49%, Holstein Red-pockmarked – 2.94%, Schwyz – 75%, Jersey – 13.79%, Red Danish – 12.5%, Red Norwegian – 20.0%, Montbellard – 25%. Among animals of Simmental and Aishir breeds – bulls with the desired complex genotype A2A2/BB were not detected. 4. Bulls of the desired complex genotype A2A2/BB according to the indicators of milk yield of daughters, the amount of milk fat, milk protein, indices of lifetime profit, profit for cheese, profit for milk were dominated bulls with separate complex genotypes CSN2-CSN3

scholarly journals THE EFFECT OF COWS GENOTYPE ON THEIR PRODUCTIVITY AND MILK QUALITY

Food systems ◽  
2018 ◽  
Vol 1 (3) ◽  
pp. 38-43 ◽  
Author(s):  
Sergey V. Tyulkin
Keyword(s):  
Milk Fat ◽  
Milk Protein ◽  
Dna Analysis ◽  
Raw Materials ◽  
Milk Proteins ◽  
Raw Milk ◽  
Milk Quality ◽  
Milk Protein Genes ◽  
Beta Casein ◽  
Beta Lactoglobulin

As studies by Russian and foreign scientists show, the breed and the cows genotype affects on their productivity and milk quality. In connection with this, the purpose of this research was to study the milk productivity and milk quality of the Tatarstan type Kholmogory cows with different complex genotypes on the milk protein genes, namely, alpha S1-casein, beta-casein, kappa-casein, beta-lactoglobulin, alpha-lactalbumin. The genotypes on the milk protein genes were determined by DNA analysis methods. Determination of quantitative and qualitative indicators of milk was carried out by control milking and on a milk analyzer «LAKTAN1–4». Better raw milk, that is, with the greatest amount of nutrients, such as milk fat and protein, was milk from cows with complex genotypes of milk proteins ВВ/АВ/АВ/АВ/АА, ВВ/АВ/АВ/АВ/АВ, ВВ/АВ/АВ/АВ/ВВ. In practical terms, it is possible to get more quality dairy products from such raw materials.

scholarly journals Relationship between genetic polymorphism of κ-casein and quantitative milk yield traits in cattle breeds and crossbreds in Serbia

Genetika ◽  
2015 ◽  
Vol 47 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Radica Djedovic ◽  
Vladan Bogdanovic ◽  
Predrag Perisic ◽  
Dragan Stanojevic ◽  
Jelena Popovic ◽  
...  
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Phenotypic Variability ◽  
Milk Proteins ◽  
Cattle Breed ◽  
Protein Composition ◽  
Polymorphism Analysis ◽  
Special Importance ◽  
Holstein Breed ◽  
Genotype Frequencies

The quality and yield of milk, and therefore of dairy products as well, depends directly on their protein composition. Milk gene proteins represent structural locuses that are functionally associated with milk production. In cattle selection a special importance is attached to the variability of kappa casein (?-CN) as one of the four casein milk proteins. Genotypes of 21 Simmental breed cows, 20 crossbred cows obtained by crossing of Simmental and Red Holstein breed, and 25 cows of autochthonous Busha breed were determined for kappa casein by restriction fragment length polymorphism analysis (PCR- RFLP) of amplified DNA. The ?-CN genotype frequencies for Simmental breed were: 42.8; 47.6 and 9.6 % for AA, and BB genotype, for crossbreds: 75.0; 25.0 and 0.0% and for Busha individuals: 41.7; 50.0 and 8.3 %, respectively. Frequencies of A and B alleles, assessed on the basis of genotypic frequencies were 0.667 and 0.333 for Simmental breed, 0.875 and 0.125 for crossbreds and 0.667 and 0.333 for autochthonous Busha cattle breed, respectively. For confirming the phenotypic variability of milk yield in standard lactation, yield of milk fat and the content of milk fat per ?-CN genotypes within breeds observed the fixed model which included the breed effect, lactation in order and effect of ?-CN genotype (AA, and BB) was used. The ?-CN genotype statistically significantly (p ?0.05) influenced milk yield and highly significantly (p ?0.01) milk fat yield, while the content of milk fat was not statistically significantly (p>0.05) influenced in examined animals.

COMPARATIVE EVALUATION OF DAIRY PRODUCTIVITY OF COWS DIFFERENT LINES IN THE CONDITIONS OF THE OREL REGION

2020 ◽  
Vol 5 (86) ◽  
pp. 73-81
Author(s):  
V.S. Buyarov ◽  
◽  
A.R. Lyashuk ◽  
◽  
◽  
...  
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Milk Protein ◽  
Correlation Coefficients ◽  
Milk Productivity ◽  
Holstein Breed ◽  
Ideal Line ◽  
The Cost ◽  
High Degree

This article provides a comparative analysis of indicators of milk productivity and quality of the main lines of the Holstein breed cows in one of the pedigree reproducers of the Orel region – "ES "Streletskoye". Among the three evaluated lines, the best results were shown by Reflection Sovereign first-calf heifers, which exceeded in milk yield the first-calf heifers of the Montvi􀉫 Chieftain line by 13.6% and the first-calf heifers of the Vis Back Ideal line by 42.3% (P≤0.001). Milk production coefficients of first-calf heifers of the Montvi􀉫 Chieftain and Reflection Sovereign lines exceeded those of the Vis Back Ideal line by 27.1% and 42.0% (P ≤ 0.001). In turn, Reflection Sovereign first-calf heifers also exceeded first-calf heifers of the Montvi􀉫 Chieftain line by 11.7% (P ≤ 0.001). Similar results were obtained for the second and third lactations. In terms of AMF and AMP, the Montvi􀉫 Chieftain and Reflection Sovereign cows significantly exceeded Vis Back Ideal cows in 305 days of each of the first three lactations. In turn, Reflection Sovereign first-calf heifers outperformed the AMF and AMP of Montvi􀉫 Chieftain first-calf heifers. The highest positive correlation coefficients between the amount of milk fat and milk yield for 305 days of lactation were found in cows of the Vis Back Ideal line - from 0.36 to 0.90 (P≤0.05–0.001). Concerning the correlation between the amount of milk protein and milk yield for 305 days of lactation, cows of all studied groups showed high levels of interdependence, characterized by a high degree of reliability - from 0.45 to 0.99 (P≤0.01– 0.001). The total cost of base fat milk produced in 305 days of the first three lactations by Montvi􀉫 Chieftain and Reflection Sovereign cows (per head) exceeded the cost of milk produced by Vis Back Ideal cows by 87.7 and 144.1 thousand rubles, respectively.

scholarly journals EVALUATION OF BULLS BY ALLELIC VARIANTS OF THE CAPA-CASEIN GENE

2020 ◽  
pp. 45-53
Author(s):  
V.I. Ladyka ◽  
Yu.I. Skliarenko ◽  
Yu.M. Pavlenko
Keyword(s):  
Milk Fat ◽  
Milk Proteins ◽  
Technological Parameters ◽  
Allele Distribution ◽  
Casein Gene ◽  
Holstein Breed ◽  
Significant Difference ◽  
Hereditary Factors ◽  
Milk Casein ◽  
Main Component

An important issue in the selection of dairy cattle today is the study of the relationship between hereditary factors that determine the types of proteins in milk. Casein is the main component of milk proteins and is represented by three fractions - alpha (CSN1S1), beta (CSN2) and kappa (CSN3). The content of individual fractions of casein depends on the breed of cows. Genetic variants of kappa-casein are associated with the quality of milk productivity of cows and greater suitability for use of milk for processing and production of cheese. The kappa-casein B allele is associated with the production of milk, the chemical composition of which is more favorable and the technological parameters are more suitable for cheese production. The aim of the work is to assess the genotype of breeding bulls by the kappa-casein gene, which were approved for use in Ukrainian farms in 2020. It was found that most breeding bulls that are approved for use are evaluated by the genotype of kappa-casein. Breeding bulls of Swiss and Jersey breeds have a higher frequency of homozygotes of explosives (100 and 86.2%, respectively). The frequency of allele distribution in the kappa-casein gene in bulls of different breeds was higher in animals of Schwyz (1.00), Jersey (0.931) and Montbeliard breeds (0.813). Among breeding bulls of Holstein breed, no significant difference in milk yield, milk fat and protein between animals of all studied genotypes by kappa-casein and average by breed (among bulls of the catalog) was found. Breeding bulls with the BB genotype had a significant difference with bulls of other genotypes in all studied traits. Therefore, the use of sperm products of bulls approved for use in 2020, with the genotype of explosives for kappa-casein will improve the economically useful traits of offspring, and will contribute to the desired genotype of cows. When choosing bulls, cheese makers should pay attention not only to protein and somatic cell count (SCS). It is also important to have a high percentage of BB cows in the herd

Milk productivity of the cows of Holstein and Simmental breeds in environment of the Novosibirsk region

2020 ◽  
pp. 41-49 ◽  
Author(s):  
A. Zheltikov ◽  
N. Kostomakhin ◽  
D. Adushinov ◽  
O. Zaiko ◽  
V. Dementev ◽  
...  
Keyword(s):  
Protein Content ◽  
Milk Yield ◽  
Milk Fat ◽  
Correlation Coefficients ◽  
Live Weight ◽  
Fat Content ◽  
Milk Productivity ◽  
Holstein Breed ◽  
Novosibirsk Region ◽  
Daily Milk Yield

The characteristic of milk productivity of cows of Holstein and Simmental breeds in LLC “Sibirskaya Niva” in the Maslyaninsky district in the Novosibirsk region has been given. It has been revealed that under the natural and climatic environments of the Western Siberia, when creating favorable conditions for feeding and housing for cows of Holstein and Simmental breeds have sufficiently high milk productivity. It has been found that Holstein first-calf heifers surpassed their Simmental herdmates by 959 kg in milk yield for the first 305 days of lactation under the same conditions of housing and feeding, by 3,1 kg in daily milk yield, by 38,2 kg in milk fat yield, and by 28,3 kg in milk protein yield (P < 0,001). There were no statistically significant differences between animals of Holstein and Simmental breeds in terms of fat and protein content in milk, which amounted to 3,94 and 2,96 % in Holstein breed and 3,97 and 2,98 % in Simmental breed, respectively. Both breeds have shown high milk productivity during the first 3 lactations. Thus, the milk yield of Holstein cows for the first 3 lactation was 6475–9290 kg, Simmental cows it was 5516–7680 kg, fat content was 3,93–3,99 and 3,96–3,97 %, respectively. The protein content in the milk of cows of both breeds did not exceed 3,0 %. High variability has been found in milk yield, yield of milk fat and protein, the coefficient of milk content and duration of lactation, the lowest in the content of protein and fat in milk and live weight. The correlation coefficients between the fat and protein content in milk were generally positive, but did not exceed 0,28. Therefore, breeding in the herd of cows in LLC “Sibirskaya Niva” have to be conducted not only by fat content, but also by protein content in milk.

scholarly journals Development of Immunohistochemical Methods for Casein Detection in Meat Products

Foods ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 28
Author(s):  
Ludmila Kalčáková ◽  
Matej Pospiech ◽  
Bohuslava Tremlová ◽  
Zdeňka Javůrková ◽  
Irina Chernukha
Keyword(s):  
Milk Protein ◽  
Production Efficiency ◽  
Raw Materials ◽  
Meat Products ◽  
Milk Proteins ◽  
Increase Production Efficiency ◽  
Allergenic Potential ◽  
Significant Difference ◽  
Dairy Ingredients ◽  
Immunohistochemical Methods

To increase production efficiency of meat products, milk protein additives are often used. Despite a number of advantages, use of dairy ingredients involves a certain risk, namely the allergenic potential of milk proteins. A number of methods have been developed to detect milk-origin raw materials in foodstuffs, including immunological reference methods. This study presents newly developed immunohistochemical (IHC) methods for casein detection in meat products. Casein was successfully detected directly in meat products where sensitivity was determined at 1.21 and specificity at 0.28. The results obtained from the IHC were compared with the Enzyme-Linked Immuno Sorbent Assay (ELISA) and there was no statistically significant difference between the IHC and ELISA methods (p > 0.05). The correspondence between the methods was 72% in total. The highest correspondence was reached in frankfurters (90%), the lowest in canned pâté (44%).

The effects on milk yield and composition of incorporating lactose into the diet of dairy cows given protected fat

1996 ◽  
Vol 62 (1) ◽  
pp. 1-3 ◽  
Author(s):  
P. C. Garnsworthy
Keyword(s):  
Sugar Beet ◽  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Protein Concentration ◽  
Milk Protein ◽  
Basal Diet ◽  
Fat Supplementation ◽  
Holstein Friesian ◽  
Group A

AbstractTwenty-eight Holstein/Friesian dairy cows were divided into four groups of seven. From weeks 4 to 15 of lactation they were given a basal diet consisting of 8 kg hay, 2 kg sugar-beet feed and 2 kg grass nuts, together with a concentrate allowance of 8 kg/day. Concentrates for group A were based on cereals and soya (control). Concentrate B contained 60 g protected fat supplement per kg; concentrate C contained 100 g lactose per kg; concentrate D contained 60 g fat supplement and 100 g lactose per kg. Milk yields were 24·6, 27·7, 25·6 and 26·5 kg/day and milk protein concentrations were 32·3, 30·7, 32·7 and 31·9 g/kg for groups A, B, C and D respectively. The effect of fat supplementation on milk yield and protein concentration was significant (P < 0·05) but the effect of lactose was not significant. Milk fat concentration was not significantly affected by treatment. It is concluded that lactose can partially alleviate the depression in milk protein concentration often observed when cows are given protected fat.

scholarly journals Effect of season, genotype and lactation on milk yield and composition of local and crossbred dairy cows reared under different feed base region

2016 ◽  
Vol 19 (1-2) ◽  
pp. 50-65
Author(s):  
MA Baset ◽  
KS Huque ◽  
NR Sarker ◽  
MM Hossain ◽  
MN Islam
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Milk Protein ◽  
Block Design ◽  
Milk Composition ◽  
Base Region ◽  
Total Solids ◽  
Randomized Block Design ◽  
Daily Milk Yield ◽  
Daily Milk

A total of 160 cows, 10 cows in each of native (local cow) and crossbred (local × Holstein Friesian) origins differing in lactation were used in 2×2×2×2 factorial experiment using Randomized Block Design (RBD) to evaluate milk yield and composition of cows considering regions (good & poor feed base region), seasons (dry: Nov.–Feb. 2009 & wet: Jun.–Oct. 2009), genotypes and lactation. A “good and/or poor feed base” region was classified based on the availability of quantity and quality roughages throughout the year. The study revealed that the daily milk yield and 4% FCM of cows under good feed base condition were 6.76 and 6.49 kg, respectively and under poor feed base condition were 3.67 and 3.31 kg, respectively. Feed base region did not affect on milk fat and it was observed that the milk protein, lactose, solids-not-fat (SNF), minerals and total solids under good feed base condition were 37.9, 54.9, 100.9, 6.3 and 140.6 g/kg, respectively, whereas, under poor feed base condition the values were 36.3, 52.9, 98.0, 6.1 and 135.2 g/kg, respectively. Season did not affect milk yield and composition except minerals (6.5 g/kg vs. 5.9 g/kg). Genotypes significantly (p?0.01) influenced daily milk yield, the milk protein and minerals. Lactation did not affect milk yield and the milk protein, but influenced the fat, lactose, SNF, minerals and total solids. The interaction of feed base regions and seasons significantly (p?0.01) influenced milk yield and the milk fat and SNF. The milk protein and lactose was influenced by the interaction of feed bases region, seasons and lactation. Milk yield negatively correlated with fat per cent. The percentage of fat significantly (p?0.01) correlated with protein, lactose, SNF, and minerals %. The percentage protein correlated with lactose, SNF and minerals. Lactose % significantly (p?0.01) correlated with SNF%. It may be concluded that milk yield and composition depends on feed base region, genotype and lactation of cows. Season did not influence milk yield and the composition. Milk yield negatively correlated with the percentage of fat, protein, lactose, SNF and milk composition strongly correlated with each other.Bangladesh J. of Livestock Res. 19(1-2): 50-65, Jan-Dec 2012

scholarly journals Structural and non-structural carbohydrates in concentrate supplements of silage-based dairy cow rations. 1. Feed intake and milk production.

1990 ◽  
Vol 38 (3B) ◽  
pp. 487-498
Author(s):  
H. de Visser ◽  
P.L. van der Togt ◽  
S. Tamminga
Keyword(s):  
Protein Content ◽  
Milk Yield ◽  
Feed Intake ◽  
Milk Fat ◽  
Milk Protein ◽  
Basal Diet ◽  
Negative Energy ◽  
Net Energy ◽  
Rumen Degradation ◽  
Silage Maize

A feeding trial was carried out with 64 multiparous dairy cows, in which the effect of type of carbohydrate in concentrate mixtures (starch vs. cell wall constituents) and differences in rumen degradation (fast vs. slow) on feed intake and milk yield were studied. The experiment started immediately after parturition and lasted for 15 wk. The basal diet, which comprised 75% of the total DM intake, consisted of wilted grass silage, maize silage and concentrates. The remaining part of the diet consisted of barley (B), maize (M), pressed ensiled beet pulp (P) or moist ensiled maize bran (MB). All diets were given as totally mixed rations. Total intake of DM and net energy did not differ between diets, but differences were found in energy partition. There was a tendency for cows given diet B to show increased liveweight gain, while cows given P mobilized more body reserves compared with the other treatments. Milk yield did not differ between diets, but milk fat content was higher for diet P. Milk protein content was higher for diets B and M compared with P and MB. The lower protein content of the milk of treatment P can be explained by a longer period of negative energy balance, while the lower milk protein in cows given diet MB probably resulted from reduced microbial protein synthesis. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Identify the Effect of Supplementing Asparagus Racemosus for Buffalo on Milk Quality Related to Milk Yield and Composition

2019 ◽  
Vol 38 (03) ◽  
Author(s):  
Birhanu Tsegaw ◽  
Rita Singh Majumdar
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Milk Protein ◽  
Treatment Phase ◽  
Total Solid ◽  
Normal Diet ◽  
Control Treatment ◽  
Asparagus Racemosus ◽  
Residual Phase ◽  
Three Phases

Milk is produced by the mammary gland of mammals to feed their young ones. Milk is an important nutrient-rich beverage that benefits our health. This article aims to study the effect of Asparagus racemosus root powder on milk yield and milk composition. The experiment was performed to determine the milk yield and milk protein, fat, total milk solid, and milk acidity after using Asparagus racemosus as a food supplement. The animals were selected based on purposive sampling to select lactate buffalo to avoid or reduced the error. During the experiment, animals fed in three phases; control, treatment, and the residual phase. In control phase animal diet normal ration, whereas in the treatment phase, a buffalo was diet 120 gram of Asparagus racemosus root powder in addition to its normal diet per buffalo per day. While in the residual phase, the animal was diet only normal ration. Each phase has been 5 days. The Milk collected from lactating buffalo in three phases; control, treatment, and the residual period. Each period has been five day. We also measured milk yield per day per buffalo. About 50 ml of milk was collected from each five buffalo every morning for 15 days. The data was analyzed through one way ANOVA and T-test using the procedure of statistical software. The result indicates that the diet was statistically significant effecting on milk yield at 5% (0.0196), protein at 1% (0.0113) and fat at 1% (0.0007) while the insignificant effect on milk acidity p >30 (0.3909) and total solid p >80 (0.8904). Therefore, feeding Asparagus racemosus, positively affects milk yield and milk protein and milk fat content.

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