Characteristics of population genetic and breeding parameters of the traits of milk productivity of black-and-white and Holstein cows of the Republic of Kazakhstan

Purpose: determination of the selection and genetic parameters of the daily milk productivity of black-and-white and Holstein cows to characterize the population.Materials and methods. The studies were carried out using a database formed on the basis of the information and analytical management system of animal husbandry in the Republic of Kazakhstan (RK) for daily control milking of Holstein and black-and-white cows. The sample included indicators of milk productivity for three lactations of animals bred in 43 herds: I lactation - 14 482 records (3130 cows and 391 bull fathers), II lactation - 13 656 records (2734 cows and 537 bull fathers), III lactation - 4,911 records (886 cows and 260 bull fathers).Results. Phenotypic variability indices (CvP) by lactation varied: daily milk yield - 34.1 ... 38.8%, MJ - 9.8 ... 11.6%, BMD - 6.7 ... 8.6%, SCS - 32.5 ... 37.8%. The additive genetic variability (CvA) was lower and ranged for: daily milk yield - 22.9 ... 27.0%, MJ - 3.8 ... 4.7%, BMD - 2.6 ... 3.1%, SCS - 13 , 8 ... 14.7%. The heritability of indicators of milk productivity on average for three lactations was: in terms of milk yield - h2 = 0.440, percentage of fat - h2 = 0.155, percentage of protein - h2 = 0.121, SCS - h2 = 0.161. Evaluation of the dynamics of changes in daily milk yield and SCS showed the standard nature of the shape of lactation curves with the values ​​of the determination coefficient of the phenotypic trend equation, respectively, R2 = 69.9 ... 86.4% and R2 = 11.8 ... 20.5%. An increase in the number of recorded days of control milking from 1 to 10 increased the accuracy of the forecast of the breeding value (EBV) of animals for signs of milk production by 1.7-2.4 times. Evaluation of cows by their own productivity for milk yield with optimal values ​​of forecast reliability over 70% can be carried out on the basis of 4 control milking units. At the same time, for the percentage of fat and protein, SCS, the number of observations to achieve reliable EBV results ranged from 8 to 10. Based on the average values ​​of the breeding value of cows, the population genetic characteristics of the farmed cattle in the Republic of Kazakhstan were determined in the context of breeds, country and region (population) origin. Holstein animals (+54.3 kg of milk) were characterized by the highest EBV values. Among imported cows, the best breeding value forecast was obtained for cows imported from Germany (+63.3 kg of milk). For the Kazakh population of cattle, leading positions in terms of genetic prognosis were achieved in East Kazakhstan region (KZF, +334.4 kg of milk). A principal component analysis based on an assessment of the breeding value of cows showed clear differentiation by breed level, country and region of origin of the livestock. The most significant differences were found for animals originated in the Kostanay region (KZP).Conclusion. Analysis of the results of population-genetic and breeding parameters of the characteristics of milk productivity of black-and-white and Holstein cattle showed that there are necessary prerequisites for the development of their own breeding base, increasing the efficiency of selection of animals for breeding groups.

Pre-breeding study for the enhancement of bioactive fruit attributes in strawberry (Fragaria x ananassa Duch.)

The strawberry fruit market increasingly demands new cultivars producing berries with enhanced bioactive attributes. In this research the suitability of twelve strawberry cultivars for effective breeding aimed at the enhancement of bioactive fruit attributes was studied. The group of genotypes with different pedigree was examined in respect of variation, breeding parameters, correlation between the content of bioactive compounds and the antioxidant capacity of fruit. Pre-breeding research showed the highly significant differences between genotypes regarding all traits analysed. Relationship between the bioactive phytochemicals content and antioxidant capacity of fruit were mostly positive and significant. Only correlation between vitamin C and flavonoids was negative (−0.482). Path analysis exhibited the highest positive direct effect of total phenolic content on antioxidant capacity (0.609). Heritability of traits was very high, reaching values above 0.90. The highest genetic advance was observed for flavonoids. Results suggested that the genotype plays the main role in shaping of fruit antioxidant potential. This study indicated that the efficient strawberry breeding focused on obtaining the forms with enhanced bioactive berry properties could be highly possible.

Unusual lack of reproduction in toad populations from agricultural habitats

Anthropogenic alterations of habitats can have detrimental consequences for biodiversity. Documenting these effects require monitoring in multiple sites that vary in the degree of alterations over long temporal scales, a task that is challenging. Yet, simple naturalist observations can reveal major ongoing events affecting wild populations, and serve as a basis for further investigations. We quantified breeding parameters of spined toad (Bufo spinosus) populations from forested (preserved) and agricultural (altered) habitats. We found that reproduction did not occur at the sites surrounded by agriculture, while it occurred successfully in ponds from forests. Males were present at all sites, but females, amplexus, egg strings and tadpoles remained absent from agricultural sites. Observations made at the same sites indicated that breeding occurred during previous years. Our observations of habitat- and sex-specific lack of reproduction may have critical consequences for the persistence of populations of a widespread amphibian species in agricultural areas.

Induced Breeding of Fresh Water Menoda Catfish (Hemibagrus Menoda) Using Ovupin Hormone

An experiment on induced breeding of menoda catfish, Hemibagrus menoda was conducted during the period from May 2017 to July 2018 by using commercial Ovupin hormone (GnRHa, Gonadotropin Releasing Hormone Analogues) to attain its optimum dose. The study consists of three treatments, each with three replications. The objective of the experiment was to find out the effective dose for induced breeding. The breeding parameters were determined in terms of ovulation, fertilization, hatching, and survival rate. A total of 36 males and 18 females lived brood fish were kept in the ratio of 2♂:1♀ for breeding purpose. The commercial Ovupin hormone were injected at the doses of 4 (T1), 6 (T2), 7 (T3) mL Ovupin·kg-1body weight for female and 1.5(T1), 2 (T2), 3(T3) mL Ovupin·kg-1 body weight for male in a triplicate replication. In this study, it was found that treatment T2 demonstrated the best spawning performance for both male and female and were statistically significant (p<0.05). The investigation indicated that the dose of Ovupin hormone 6mL Ovupin·kg-1body weight for female and 2 mL Ovupin·kg-1 body weight for male determines the highest spawning performance of menoda catfish which should be recommended for high quality eggs and larvae. J. Biodivers. Conserv. Bioresour. Manag. 2020, 6(2): 11-18

Ecological and Genetic Monitoring of a Recently Established Osprey (Pandion haliaetus) Population in Wales

ABSTRACT The Osprey (Pandion haliaetus) experienced severe declines in Europe over the past centuries, including extirpations from large parts of its range. After Ospreys began naturally recolonizing Wales, United Kingdom (UK), in 2004, the Dyfi Osprey Project initiated an extensive ecological (ringing, satellite tracking, and nest video recordings) and genetic (microsatellite genotyping) monitoring program alongside artificial nest platform construction. From 2004 to 2019 there were 40 reproductive events (i.e., eggs laid in a nest) across six nests, resulting in a total of 95 fledglings and a yearly average of 2.35 ± 0.8 (SD) fledglings per nest-with-eggs. Video recording at one nest documented high hatching success and survival to the fledging and dispersal phases, as well as mate- and nest-fidelity across multiple years. Collectively these breeding parameters suggest high productivity associated with artificial nest structures and limited density-dependent effects for the Welsh population due to its small size and low density at this stage of recovery. Satellite tracking of four migrating individuals revealed that Ospreys used a western European migratory flyway and wintered in sub-Saharan western Africa. Genetic analysis of nestlings from five nests indicated single paternity in all cases, in agreement with evidence of social monogamy. Genetic analysis of geographical outgroups (Scottish, Swedish, and Latvian) provided preliminary evidence of cryptic population structure among UK (Welsh and Scottish) populations. The novel combination of loci show Welsh Osprey retain high levels of variation and more broadly direct reconsideration of the hitherto inferred genetic poverty of the species compared to other raptors. We recommend implementation of similar long-term ecological and genetic monitoring programs for other populations.

Comparative Transcriptome Analysis of Milk Somatic Cells During Lactation Between Two Intensively Reared Dairy Sheep Breeds

In dairy sheep industry, milk production dictates the value of a ewe. Milk production is directly related to the morphology and physiology of the mammary gland; both being designated targets of breeding strategies. Although within a flock breeding parameters are mutual, large differences in milk production among individual ewes are usually observed. In this work, we tested two of the most productive dairy sheep breeds reared intensively in Greece, one local the Chios breed and one foreign the Lacaune breed. We used transcriptome sequencing to reveal molecular mechanisms that render the mammary gland highly productive or not. While highly expressed genes (caseins and major whey protein genes) were common among breeds, differences were observed in differentially expressed genes. ENSOARG00000008077, as a member of ribosomal protein 14 family, together with LPCAT2, CCR3, GPSM2, ZNF131, and ASIP were among the genes significantly differentiating mammary gland’s productivity in high yielding ewes. Gene ontology terms were mainly linked to the inherent transcriptional activity of the mammary gland (GO:0005524, GO:0030552, GO:0016740, GO:0004842), lipid transfer activity (GO:0005319) and innate immunity (GO:0002376, GO:0075528, GO:0002520). In addition, clusters of genes affecting zinc and iron trafficking into mitochondria were highlighted for high yielding ewes (GO:0071294, GO:0010043). Our analyses provide insights into the molecular pathways involved in lactation between ewes of different performances. Results revealed management issues that should be addressed by breeders in order to move toward increased milk yields through selection of the desired phenotypes. Our results will also contribute toward the selection of the most resilient and productive ewes, thus, will strengthen the existing breeding systems against a spectrum of environmental threats.

Endemic Cyprus Scops Owl Otus cyprius Readily Breeds in Artificial Nest Boxes

As is well-known, endemic island bird species are especially vulnerable to extinction from anthropogenic environmental change and reduced fitness compared with mainland taxa. The Cyprus Scops Owl, Otus cyprius, is a recently recognized island endemic species whose ecology and breeding biology have not been studied. It nests mainly in holes in trees and buildings, so the felling of old trees, modern architectural practices, and the renovation of old houses in villages may reduce nest site availability. Its population trend is also unknown. Therefore, to better determine its ecological requirements and habitat preferences we placed nest boxes in rural areas adjacent to the forest, in the forest, and in the ecotone between them, and used breeding success as our indicator of habitat suitability. We found that breeding parameters like laying date, clutch size, length of the incubation period, hatching day, hatching success, and number of nestlings did not differ between the three habitats. Despite the low level of nest box occupancy rate (5–11%) the endemic Cyprus Scops Owl readily breeds in artificial nests. Therefore, although we are unaware of any current threats to the Cyprus Scops Owl, we recommend that its conservation be prioritized, including studies, monitoring, habitat conservation, and the provision of nest boxes.

Landfast ice: a major driver of reproductive success in a polar seabird

In a fast-changing world, polar ecosystems are threatened by climate variability. Understanding the roles of fine-scale processes, and linear and nonlinear effects of climate factors on the demography of polar species is crucial for anticipating the future state of these fragile ecosystems. While the effects of sea ice on polar marine top predators are increasingly being studied, little is known about the impacts of landfast ice (LFI) on this species community. Based on a unique 39-year time series of satellite imagery and in situ meteorological conditions and on the world's longest dataset of emperor penguin ( Aptenodytes forsteri ) breeding parameters, we studied the effects of fine-scale variability of LFI and weather conditions on this species' reproductive success. We found that longer distances to the LFI edge (i.e. foraging areas) negatively affected the overall breeding success but also the fledging success. Climate window analyses suggested that chick mortality was particularly sensitive to LFI variability between August and November. Snowfall in May also affected hatching success. Given the sensitivity of LFI to storms and changes in wind direction, important future repercussions on the breeding habitat of emperor penguins are to be expected in the context of climate change.

Breeding Ecology and Nest- Site Selection of Turtle Doves (Streptopelia turtur) in Three New Orchard Habitats

The main aim of our work was to investigate the breeding parameters in three orchard types in Algeria (apple, cherry and nectarine) for better conservation of existing species. A total of 149 active Turtle dove nests were monitored in these man–made agro-systems. Egg laying occurred from early May and continued until mid-August. Egg laying started later in cherry trees and stopped earlier in nectarines. Nest density was higher in apple orchards. Nests were located higher in nectarine. Clutch size was similar among orchard types. Northeast was the dominant orientation in all orchards. Breeding success was higher than that recorded in former studies. Desertion was the main cause of nest failure. The apple orchards are the preferred breeding area for turtle dove in this region.