scholarly journals Callus Induction from Leaf and Stem Explants of Selected Turkish Genotypes of Common Bean

2021 ◽  
Vol 5 (11) ◽  
pp. 02-05
Author(s):  
Asli Küçükrecep ◽  
Dilek Tekdal
Keyword(s):  
Common Bean ◽  
Callus Induction ◽  
Stem Explants

scholarly journals Role of Growth Regulators on In Vitro Callus Induction and Direct Regeneration in Physalis minima Linn

2015 ◽  
Vol 44 ◽  
pp. 38-44 ◽  
Author(s):  
H. Sandhya ◽  
Rao Srinath
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Callus Induction ◽  
Basal Medium ◽  
Direct Regeneration ◽  
Naphthalene Acetic Acid ◽  
Stem Explants ◽  
Dichlorophenoxy Acetic Acid ◽  
Indole 3 Acetic Acid

Suitable protocol for induction of callus and regeneration was developed from different explants viz., node, stem and leaves in Physalis minima. MS basal medium supplemented with various concentrations (1.0-4.0mg/l) of auxins like 2,4-Dichlorophenoxy acetic acid (2,4-D), α-naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA) and cytokinins (0.5-1.5mg/l) like BAP or Kn were used. All the three explants responded for induction of callus, however stem explants were found superior, followed by node and leaf. Callus induction was observed in all the auxins and combination of growth regulators used with varied mass (2010±1.10) and highest percentage of callus induction was observed from stem at 2.0mg/l 2,4-D (90%) followed by NAA (70%) and IAA (50%). Organogenesis was induced when nodal explants were transferred on MS medium supplemented with 2,4-D and Kn at various concentrations, maximum being on 2.0mg/l 2,4-D + 1.0mg/l Kn (90%). Regenerated shoots were elongated on 0.5mg/l GA3. The shoots were subsequently rooted on MS + 1.0mg/l IBA (95%) medium. Rooted shoots were hardened and acclimatized, later they were transferred to polycups containing soil, cocopeat and sand in the ratio 1:2:1.Keywords:Physalis minima, Node, Stem, Leaf, callus and growth regulators.

scholarly journals IN VITRO CALLUS INDUCTION AND COMPARATIVE GC-MS ANALYSIS OF METHANOLIC EXTRACTS OF CALLUS AND LEAF SAMPLES OF AMPELOCISSUS LATIFOLIA (ROXB.) PLANCH

2018 ◽  
Vol 10 (9) ◽  
pp. 68
Author(s):  
Deep Chhavi Anand ◽  
Rishikesh Meena ◽  
Vidya Patni
Keyword(s):  
Callus Induction ◽  
Culture Media ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Dodecanoic Acid ◽  
Stem Explants ◽  
Ms Analysis ◽  
Wide Range

Objective: The aim of the present study was to develop a callus induction protocol and comparative study of therapeutic phytochemicals present in in vivo leaf and in vitro callus extracts through Gas Chromatography-Mass Spectrometry analysis.Methods: Murashige and Skoog media was used as culture media for callus induction. In vitro callus induction protocol was developed by studying the effects of various plant growth regulators like auxin, 2, 4-D (2,4-dichlorophenoxyacetic acid), NAA (naphthalic acetic acid), alone and in combination with cytokinin BAP (benzyl aminopurine), on leaf and stem explants. The GC-MS analysis of Ampelocissus latifolia was carried out on Shimadzu QP-2010 plus with thermal desorption system TD 20 to study the phytochemical profile.Results: In vitro callus induction protocol was developed for the plant and callusing was done from leaf and stem explants of Ampelocissus latifolia. The best result for callus induction was obtained using leaf explant, and callus production were maximum in Murashige and Skoog medium fortified with BAP (0.5 mg/l) and NAA (1.0 mg/l). Major compounds identified in the GC-MS analysis were Campesterol, Stigmasterol, Beta-Sitosterol, Docosanol, Dodecanoic acid, etc., in in vitro extract and Beta Sitosterol, Tocopherol, Squalene, Bergamot oil, Margarinic acid, Hexadecanoic acid, etc., in in vivo extract. The different active phytochemicals identified have been found to possess a wide range of biological activities, thus this analysis forms a basis for the biological characterization and importance of the compounds identified for human benefits.Conclusion: This is the first report on callus induction in Ampelocissus latifolia. From the results obtained through the in vitro callus induction and its comparative GCMS analysis with in vivo extract, it is revealed that Ampelocissus latifolia contains various bioactive compounds that are of importance for phytopharmaceutical uses. The GCMS analysis revealed that the amount of Beta-sitosterol and 5-Hydroxymethylfurfural (HMF) was very high in in vitro extract as compared to in vivo extract.

Production of Glycyrrhizin in Callus Cultures of Licorice

2008 ◽  
Vol 63 (5-6) ◽  
pp. 413-417 ◽  
Author(s):  
Winida Wongwicha ◽  
Hiroyuki Tanaka ◽  
Yukihiro Shoyama ◽  
Indree Tuvshintogtokh ◽  
Waraporn Putalun
Keyword(s):  
Callus Induction ◽  
Shoot Formation ◽  
Callus Cultures ◽  
Glycyrrhiza Glabra ◽  
Naphthaleneacetic Acid ◽  
Stem Explants ◽  
Ms Medium ◽  
Shoot Induction

Licorice plants, Glycyrrhiza glabra, G. uralensis, and G. inflata, were investigated for callus induction using Murashige and Skoog (MS) medium combined with auxins and cytokinins. After 4 weeks of culture, 33-100% of leaf or stem explants formed calli. Maximum of shoot induction from callus cultures was achieved by G. inflata stem explants cultured on MS medium supplemented with 1 mg/l α-naphthaleneacetic acid (NAA) and 0.5 mg/l 6-benzyladenine (BA) (67%) which also gave maximum shoot formation per explant (two shoots per explant). These results indicated that all three Glycyrrhiza species regenerated shoots from callus cultures on MS medium combined with NAA and BA or only thidiazuron (TDZ; 0.1 and 0.5 mg/l). Glycyrrhizin contents of G. uralensis calli induced using MS medium in combination with NAA and BA [(27.60 ± 8.47) μg/g DW] or TDZ alone [(36.52 ± 2.45) μg/ g DW] were higher than those found in other combinations.

Production of Isoflavonoids in Callus Cultures of Pueraria candollei var. mirifica

2009 ◽  
Vol 64 (3-4) ◽  
pp. 239-243 ◽  
Author(s):  
Latiporn Udomsuk ◽  
Kanokwan Jarukamjorn ◽  
Hiroyuki Tanaka ◽  
Waraporn Putalun
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Culture Medium ◽  
Callus Cultures ◽  
Stem Explants ◽  
Ms Medium ◽  
Shoot Induction ◽  
Pueraria Candollei

Pueraria candollei Wall. ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66 - 100% of leaf or stem explants formed calli. Calli from stem explants cultured on MS medium supplemented with 0.5 mg/l thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem explants of P. candollei var. mirifica.

scholarly journals An efficient protocol for mass multiplication of Centella asiatica (L.) Urban and determination of its phenolic content

2021 ◽  
pp. 233-239
Author(s):  
Shweta Kumari ◽  
Maheshwar Prasad Trivedi
Keyword(s):  
Content Analysis ◽  
Callus Induction ◽  
Phenolic Content ◽  
Leaf Explants ◽  
Centella Asiatica ◽  
Stem Explants ◽  
Half Strength ◽  
Maximum Root Length ◽  
Maximum Root

The present study was focused on standardizing a protocol for callus induction as well as regeneration in Centella asiatica from leaf and stem as explants. Stem and leaf explants have been inoculated in B5 media supplemented with BAP (0.1-2.5 mg/l), kn (01-04 mg/l) and NAA (0.1-0.5 mg/l), 2, 4-D (0.2mg/l) for callus induction. The combination of BAP and NAA leads to the formation of green, brown, compact and friable calli while Kn and 2, 4-D induced brown calli. Highest shooting was obtained from BAP (1.5 mg/l) and NAA(0.5 mg/l).When the shoots were inoculated in half strength of B5 media fortified with 0.1 mg/l BAP and 0.5 mg/l NAA showed cent percent rooting with the highest number of roots per shoot (11.05 cm) and maximum root length (1.86 cm). Stem showed the best explants for callus induction as compared to leaf explants. A low concentration of plant growth regulators was unable to induced callus response in leaf and stem explants. Phenolic content analysis showed that calluses contain more amounts of phenol (0.81 mg/gmdw) as compared to both leaf (0.63 mg/gmdw) and stem (0.59 mg/gmdw) explants.

scholarly journals Callus Derived Regeneration of Some Selected Brassica Genotypes

2018 ◽  
Vol 15 (2) ◽  
pp. 1-10
Author(s):  
S K Biswas ◽  
M Z Tareq ◽  
S Ahmmed ◽  
A B M Z Hoque ◽  
M T Rahman
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Brassica Species ◽  
Direct Organogenesis ◽  
Stem Explants ◽  
Root Induction ◽  
Ms Medium ◽  
Cotyledon Explants ◽  
Randomized Design ◽  
Completely Randomized Design

The experiment was conducted to observe the callus induction ability of Brassica species. Plantlets were regenerated from cotyledon and stem explants of Brassica napus, Brassica campestris and Brassica juncea through direct organogenesis. The experiments were conducted in a Completely Randomized Design (CRD) with 4 replications. The highest frequency of callus formation was recorded in MS containing 2.0 mgl-1 BAP, 0.5 mgl-1 NAA and 2.0 mgl-1 AgNO3 in both stem and cotyledon explants. Among these explants, stem was found to be better responsive in callus induction than cotyledon. Among the genotypes used, BINA Sarisha-4 induced the highest percentage (100.00%) of callus from stem explants which was followed by BINA Sarisha-5 (100.00%) and Sampad (83.35%). On the other hand, BINA Sarisha-4 induced callus from 91.67% cotyledon explants, followed by BINA Sarisha-5 (75.00%) and Sampad (66.67%). Similarly, the highest percentage of shoot regeneration (58.34%) from stem explants of BINA Sarisha-4 was observed in MS medium supplemented with combination of hormone and silver nitrate concentrations. The highest percentage of root induction was 66.67 and 58.33% in plantlets derived from stem and cotyledon explants, respectively in ½ MS medium supplemented with 2.0 mgl-1 IBA and 0.5 mgl-1 of NAA. The highest survival rate was found after acclimatization of plants derived from stem (77.78%) and cotyledon (64.28%) explants of BINA Sarisha-4 in pot and 64.33 and 55.55%, respectively in field.The Agriculturists 2017; 15(2) 01-10

scholarly journals In vitro regeneration protocol development via callus formation from stem explant of tomato

2016 ◽  
Vol 1 (3) ◽  
pp. 589-599
Author(s):  
Meherunnesa Papry ◽  
SM Ahsan ◽  
Sayeed Shahriyar
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Germination Rate ◽  
Plant Biotechnology ◽  
Stem Explants ◽  
Ms Medium ◽  
Good Efficiency ◽  
Shoot Initiation

The experiment was conducted on in vitro regeneration of tomato at the Plant Biotechnology Laboratory, Department of Horticulture, Patuakhali Science and Technology University, Patuakhali. The objective was to develop an efficient regeneration protocol in tomato through callus induction for subsequent plantlet regeneration. Seeds were inoculated on MS medium where germination rate was 78.4%. The stems of in vitrocultured seedlings were used as explants. Different concentrations and combinations of growth regulators were added to MS medium to observe their efficacy on callus induction, shoot initiation and root formation. Stem explants cultured on MS medium fortified with 2 mg/L BAPgave the highest number of shoots (3.0) at 45 DAC. Among the concentrations of PGRs, 0.25 mg/L IAA produced the highest length (4.064 cm) of plantlets, number (5.0) of leaves and fresh weight (0.663 g) of plantlets with the stem explants at 45 DAC. The concentration of 0.5 mg/L IAA produced the highest number (21.00) of roots/plantlet, length (7.676 cm) of roots at 45 DAC, from the same explants. The highest survival rate of in vitro regenerated plantlets in the pot was 70.00 % with the stem explants. The results of the current study showed significant increase in the growth of callus of Solanumlycopersicon Mill. Indicating a good efficiency of the optimized media composition and the experimental model used in comparison to other studies of similar nature.Asian J. Med. Biol. Res. December 2015, 1(3): 589-599

scholarly journals In vitro Regeneration of Alstroemeria cv. ‘Balance’ by Indirect Organogenesis

2017 ◽  
Vol 14 (2) ◽  
pp. 607-614
Author(s):  
Hossein Nazarian ◽  
Maryam Beigi Harchegani ◽  
Mahmoud Otroshy ◽  
Ali Motamedi
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Culture Technique ◽  
Stem Explants ◽  
Indirect Organogenesis ◽  
High Concentrations ◽  
Two Phases ◽  
Positive Effect

ABSTRACT: This study was designed in order to optimize the indirect organogenesis (during callus induction and regeneration) of Alstroemeria cv. ‘Balance’ through tissue culture technique in two phases; the first stage: callus induction by rhizome segments, leaf and nodal stem which in the start, callus formation media were examined using two types of auxins; 2,4-D and NAA and a cytokinin; BAP in four different experimentations. In the second stage, calli derived from rhizome segments and nodal stem explants were transferred to regeneration media. The results revealed that 2,4-D in combination with BAP in the rhizome segments and nodal stem explants were efficient as compared to NAA. The highest yield of callus formation was also obtained in the rhizome segments explants. According to the results, it can be suggested that NAA as auxin, does not have direct positive effect on cell division in Alstroemeria. The 2,4-D is toxic at high concentrations and may bring about cell death. Eventually, the composition of 0.5 mg/l NAA with 3 mg/l BAP and callus derived from nodal stem explants may be introduced as the best combination for regeneration. These results indicate the necessity of the BAP cytokinin presence for regeneration. In addition, the maximum length of the shoot was obtained from combination of BAP with nodal stem explants, without the presence of NAA.

scholarly journals 060 Differential Responses of Egyptian Faba Bean Genotypes to in Vitro Callus Induction

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 398E-398
Author(s):  
F. Nekouei ◽  
J.O. Kuti
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Callus Induction ◽  
Callus Cultures ◽  
Stem Explants ◽  
In Vitro Screening ◽  
Leaf Petiole ◽  
The Media ◽  
Agar Media

Callus induction in 12 genotypes of faba bean (Vicia faba L.) genotypes from Egypt were examined. Cotyledon, leaf petiole, and stem explants were cultured on two basal agar media; Murashige and Skoog (MS) and Gamborg (B5). The media contained 0.5 mm 2,4-D, 0.25 mm NAA, and 30 g of sucrose/L. Calli were easily formed in B5 media and induction rate was significantly dependent on the genotype. The highest induction rates occurred mostly in genotypes from Assiut Univ., Egypt, and in a local variety `Goya'. These callus cultures will be used for in vitro screening of the faba bean genotypes for tolerance to salt and drought, respectively.

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