scholarly journals Levamisole usage for the block of intestinal alkaline phosphatase in immunohistochemical staining

2020 ◽  
Vol 9 (3) ◽  
pp. 74-79
Author(s):  
N.A. Oleynikova ◽  
O.A. Kharlova ◽  
N.V. Danilova ◽  
I.A. Mikhailov ◽  
P.G. Malkov
Keyword(s):  
Colon Cancer ◽  
Alkaline Phosphatase ◽  
Immunohistochemical Staining ◽  
Cost Benefit Analysis ◽  
Research Work ◽  
Cost Benefit ◽  
Intestinal Alkaline Phosphatase ◽  
Background Staining ◽  
Solution Preparation ◽  
Immunohistochemical Studies

Introduction. Immunohistochemical staining by an indirect method with a chromogenic label requires en-zymes, among which is alkaline phosphatase (AP). AP is also present in human tissues. It can break down the molecules of the immunohistochemical substrate, which leads to significant background staining. It is necessary to block endogenous enzymes prior to immunohistochemical staining to reduce this effect. One of the ways to block endogenous alkaline phosphatase is to use levamisole solutions. The aim of the study was to describe the technique of levamisole use to block the alkaline phosphatase intestinal form during immunohistochemical assays. Materials and methods. This article provides the calculations for 0.001M working levamisole solution preparation from a 10% officinal veterinary levamisole hydrochloride produced by Livisto Invesa Industrial Veterinaria S. A., Spain. The inactivation of AP intestinal form was checked by a reaction with one marker (PDGFRb) and two markers (FAP and SMA) on the colon cancer specimens. We used the Abcam ab210061 DoubleStain IHC Kit: M&R on human tissue (HRP/Green&AP/Red, Great Britain) according to the method recommended by the manufacturer with some changes to identify two markers on the same slide. Results. During the immunohistochemical assay, a complete absence of background staining and a bright contrast reaction with antibodies (both with one and two in the same section) using 1mМ levamisole was achieved. It indicates sufficient inactivation of the AP intestinal isoform in the colon cancer specimens. A comparative cost-benefit analysis for one slide using ready-to-use commercial blocking reagents and officinal levamisole solution shows a significant economic advantage of the latter. Conclusion. The high reaction quality and the palpable economic profits open up opportunities for using 1mM levamisole solution for immunohistochemical studies in laboratory practice and research work. Keywords: levamisole, immunochistochemistry, alkaline phosphatase

scholarly journals Induced breeding of Vietnames koi (Anabas testudineus) using pituitary gland (PG) and ovaprim at Tasty Fish and Feeds Industries Limited, Melandah, Jamalpur

2016 ◽  
Vol 1 (3) ◽  
pp. 607-611
Author(s):  
Md Ruhul Amin ◽  
Md Mamunur Rahman ◽  
Sopna Aktar ◽  
Md Badrul Alam ◽  
Md Ashraful Alam
Keyword(s):  
Body Weight ◽  
Cost Benefit Analysis ◽  
Survival Rates ◽  
Research Work ◽  
Cost Benefit ◽  
Benefit Analysis ◽  
Breeding Performance ◽  
Anabas Testudineus ◽  
Brood Rearing ◽  
The Cost

The research work was conducted for induced breeding of Vietnames Koi (Anabas testudineus) using carpPG extract and Ovaprim hormone at Tasty Fish and Feeds Industries Limited, Melandah, Jamalpur during March to May, 2015. Brood fishes were reared for 4-5 months in the brood rearing pond by providing farm-made artificial feed (containing 32% protein) for maturation before onset of breeding season. Two different hormones i.e., PG-PG (both for male and female), and PG-Ovaprim (PG for male and Ovaprim for female) were used as inducing agents for the purpose of induction of breeding A. testudineus. The broods were induced with PG-PG hormone at the doses of 2 mg PG/Kg body weight of male and 7 mg PG/Kg body weight of female and PG-Ovaprim hormone were used at the doses of 2 mg PG/Kg body weight of male and 7 ml Ovaprim/Kg body weight of female and designated as treatments T1 and T2, respectively. Ovulation rates (100%) were similar in both the treatments, whereas, fertilization, hatching, and survival rates were 78.11±3.24%, 70.22±3.40%, and 51.22±3.96% in T1 and 71.00±3.97%, 60.89±4.20%, and 48.89±4.41% in T2, respectively. Although comparatively better breeding performance was found for carp PG extract but these values were not significantly higher compared to Ovaprim. Therefore, comparing the breeding performance and considering the cost-benefit analysis it is suggested that Ovaprim could be used as an alternative of carp PG extract for the induced breeding of Vietnames koi.Asian J. Med. Biol. Res. December 2015, 1(3): 607-611

Self-Expanding Metallic Stent as a Bridge to Surgery in the Treatment of Left Colon Cancer Obstruction: Cost–Benefit Analysis and Oncologic Results

2017 ◽  
Vol 95 (3) ◽  
pp. 143-151
Author(s):  
Blas Flor-Lorente ◽  
Gloria Báguena ◽  
Matteo Frasson ◽  
Alvaro García-Granero ◽  
Andrés Cervantes ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cost Benefit Analysis ◽  
Cost Benefit ◽  
Left Colon ◽  
Metallic Stent ◽  
Bridge To Surgery ◽  
Benefit Analysis ◽  
Oncologic Results ◽  
Left Colon Cancer

scholarly journals Uncertainty analysis in sizing rainwater harvesting tanks in an isolated island with limited water resources

2018 ◽  
Vol 20 (3) ◽  
pp. 534-540 ◽  
Keyword(s):  
Uncertainty Analysis ◽  
Rainwater Harvesting ◽  
Cost Benefit Analysis ◽  
High Reliability ◽  
Research Work ◽  
Cost Benefit ◽  
Balance Model ◽  
Surface Areas ◽  
Water Transportation ◽  
Harvesting Systems

<p>This research work focuses on the analysis of the involved uncertainty and the corresponding reliability in the sizing of rainwater harvesting systems. For this reason, an uncertainty analysis was carried out, based on 23 years of historic daily record of a station in Kimolos Island (Aegean, Greece). In order to produce the synthetic daily timeseries, a disaggregation procedure was followed. The resulting dataset was used in a rainwater harvesting tank balance model for the optimal sizing of the system. Three representative timeseries, as well as the historic one, were selected for further investigation, concerning two different scenarios. The results show that a rainwater harvesting system in the island of Kimolos does not show great reliability for small collection surface areas. For high reliability, relatively large water collection areas and lower daily water consumptions are required. A cost-benefit analysis was also conducted, which shows that a rainwater harvesting system is advantageous considering the state’s expenses for water transportation, but it is profitless for households, based on current pricing of water.</p>

Developing an In-House Biological Safety Cabinet Certification Program at the University of North Dakota

2019 ◽  
Vol 24 (3) ◽  
pp. 153-160 ◽  
Author(s):  
Sumit Ghosh ◽  
Jeffrey Voigt ◽  
Terrance Wynne ◽  
Terrance Nelson
Keyword(s):  
North Dakota ◽  
Cost Benefit Analysis ◽  
Research Work ◽  
Cost Benefit ◽  
National Standards ◽  
Certification Program ◽  
University Of North Dakota ◽  
Biological Safety ◽  
Primary Means ◽  
The University

Introduction:Biological safety cabinets (BSCs) are the primary means of containment used in laboratories worldwide. To ensure the proper functioning of BSCs, they need to be certified annually, at a minimum, per National Sanitation Foundation (NSF)/American National Standards Institute Standard 49.Objectives:A common problem most organizations face is that in many instances, the technicians who certify the cabinets are not accredited by the NSF. Additionally, in states or regions that do not have local NSF accredited field certifiers, it takes weeks to get a service request completed, thereby delaying the research work of the laboratory. Moreover, in such instances, the cost associated with cabinet certification and repair can be very high.Materials and Methods:This led the Office of Safety at the University of North Dakota to do a thorough cost-benefit analysis of developing an in-house BSC certification program. After completing the training and testing requirements for the NSF’s advanced accreditation program, the BSC certification program was initiated on campus.Results:The identified benefits led to the initiation of a program in both local and regional capacity for repair, maintenance, and certification of BSCs, and the university’s experiences were shared with other universities.Conclusions:By developing an in-house BSC certification program, the University of North Dakota was able to reduce wait times associated with service repairs, reduce costs, and generate revenue for the department. Furthermore, this led to improved hands-on training programs related to BSC use in laboratories working with biohazardous agents.

scholarly journals Lizard meal as protein source enhanced broiler growth and reduced cost of production in Ishiagu, Ivo Local Government Area, Ebonyi State, Nigeria

2020 ◽  
Vol 44 (5) ◽  
pp. 99-103
Author(s):  
A. D. Olabode ◽  
L. C. Ugwuowo ◽  
C. I. Agu ◽  
T. J. Ojuoloruntaye
Keyword(s):  
Treatment Group ◽  
Fish Meal ◽  
Cost Benefit Analysis ◽  
Research Work ◽  
Cost Benefit ◽  
Average Weight ◽  
Feed Conversion ◽  
Final Body Weight ◽  
Average Weight Gain ◽  
Better Than

The research work was carried out to ascertain the performance and cost benefits of broiler starter birds fed replacement levels of lizardmeal to fishmeal at starter phase.Atotal number of 90 day oldAnak 2000 broiler chickswere used for the experiment. The birdswere assigned to five treatment group of eighteen birds each been replicated thrice with six birds per replicate. Lizard meal was included to replace fish meal at levels of 0, 25, 50, 75 and 100% respectively. Feed and water were given ad-libitum. Birds fed 75% inclusion levels of lizard meal (treatment 4) to replace fish meal was superior to birds in other treatments in terms of average final body weight, average daily feed intake, average weight gain and feed conversion ratio. Cost benefit analysis also showed that birds in treatment 4 (75% lizard meal inclusion)was better than the rest of the treatment group.

scholarly journals Cost–benefit analysis of flood mitigation measures: a case study employing high-performance hydraulic and damage modelling

2021 ◽  
Author(s):  
Daniela Molinari ◽  
Susanna Dazzi ◽  
Edoardo Gattai ◽  
Guido Minucci ◽  
Giulia Pesaro ◽  
...  
Keyword(s):  
High Performance ◽  
Cost Benefit Analysis ◽  
Research Work ◽  
Cost Benefit ◽  
Flood Mitigation ◽  
Mitigation Measures ◽  
Computational Time ◽  
Micro Scale ◽  
Damage Models

AbstractThis paper shows a detailed, advanced procedure to implement cost–benefit analyses (CBAs) in order to assess the effectiveness of flood mitigation measures. The town of Lodi (North of Italy) has been selected as a case study for the research work, as it was hit by a large flood in 2002 for which several data are available. In order to compute the benefits, in terms of avoided damage with the mitigation measure in place, micro-scale damage models developed within the Flood-IMPAT + project were used. The great amount of input data for such models comes from results of a two-dimensional river modelling, for what concern the hazard parameters, and from open-source database, to evaluate the vulnerability and the exposure of the hit area. The research highlights that technological-advanced, high-performance hydraulic models allow taking into account a variety of hazard scenarios, with reasonable computational time, supporting the proper accounting of the probabilistic nature of risk in CBAs. Nonetheless, such high-resolution tools support the implementation of micro-scale damage assessment models, which can provide information on the distribution of benefits in the investigated area, increasing the effectiveness of CBAs for policy making.

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