Prevalence of quinolone resistance determinantqnrA6 among broad- and extended-spectrum beta-lactam-resistant Proteus mirabilis and Morganella morganii clinical isolates withsul1-type class 1 integron association in a Tunisian Hospital

2013 ◽  
Vol 45 (8) ◽  
pp. 600-605 ◽  
Author(s):  
Sihem Mahrouki ◽  
Mariagrazia Perilli ◽  
Amel Bourouis ◽  
Hela Chihi ◽  
Mustapha Ferjani ◽  
...  
Keyword(s):  
Proteus Mirabilis ◽  
Quinolone Resistance ◽  
Clinical Isolates ◽  
Beta Lactam ◽  
Morganella Morganii ◽  
Class 1 Integron ◽  
Extended Spectrum ◽  
Class 1

scholarly journals Chromosome-Encoded AmpC and CTX-M Extended-Spectrum β-Lactamases in Clinical Isolates ofProteus mirabilisfrom Korea

2011 ◽  
Vol 55 (4) ◽  
pp. 1414-1419 ◽  
Author(s):  
Wonkeun Song ◽  
Juwon Kim ◽  
Il Kwon Bae ◽  
Seok Hoon Jeong ◽  
Young Hee Seo ◽  
...  
Keyword(s):  
Chromosomal Location ◽  
Clinical Isolates ◽  
Class 1 Integron ◽  
Extended Spectrum ◽  
Hospital Environments ◽  
Class 1 ◽  
Genes Encoding ◽  
Unusual Phenomenon ◽  
M 12 ◽  
Lactamase Gene

ABSTRACTAmong 222Proteus mirabilisclinical isolates collected from 17 hospitals in Korea in 2008, 28 (12.6%) and 8 (3.6%) isolates exhibited extended-spectrum β-lactamase (ESBL) and AmpC phenotypes, respectively. The most common type of ESBL gene identified by PCR and sequencing experiments wasblaCTX-M-14a(n= 12). TheblaCTX-M-90(n= 4),blaCTX-M-15(n= 3),blaCTX-M-12(n= 3),blaCTX-M-2(n= 2),blaCTX-M-14b(n= 1),blaTEM-52(n= 5), andblaSHV-12(n= 1) genes were also detected. Eight isolates carried an AmpC β-lactamase gene, such asblaCMY-2(n= 6) orblaDHA-1(n= 2). Allblagenes encoding CTX-M-1- and CTX-M-9-type enzymes and allblaCMY-2genes were preceded by ISEcp1-like elements. TheblaCTX-M-2gene found in two isolates was located on a complex class 1 integron. TheblaDHA-1gene was preceded by a transcriptional regulator gene and was followed by phage shock protein genes. TheblaCTX-Mgenes were located on the chromosome in 21 isolates. A plasmid location for theblaCTX-Mgene was found in only four isolates: theblaCTX-M-14agene was located on ∼150-kbp IncA/C plasmids in three isolates and on a ∼50-kbp IncN plasmid in one isolate. TheblaTEM-52gene was located on ∼50-kbp IncN plasmids in all five isolates. The AmpC β-lactamase genes were located on the chromosome in seven of eight isolates; one isolate carried theblaCMY-2gene on a ∼150-kbp IncA/C plasmid. Our results show that a chromosomal location of CTX-M ESBL and AmpC β-lactamase genes inP. mirabilisis no longer an unusual phenomenon in hospital environments.

scholarly journals Prevalence of Class 1 Integron among Multidrug-Resistant Acinetobacter baumannii in Tabriz, Northwest of Iran

2012 ◽  
Vol 61 (1) ◽  
pp. 57-60 ◽  
Author(s):  
AMIR PEYMANI ◽  
SAFAR FARAJNIA ◽  
MOHAMMAD REZA NAHAEI ◽  
NASROLLAH SOHRABI ◽  
LALEH ABBASI ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Beta Lactam ◽  
Class 1 Integron ◽  
First Report ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Northwest Of Iran ◽  
Pcr Method

Integrons are associated with a variety of gene cassettes, which confer resistance to multiple classes of antibacterial drugs. In this study we tested the frequency of class 1 and 2 integrons among multidrug-resistant Acinetobacter baumannii (MDRAB) clinical isolates. One hundred clinical isolates of A. baumannii were screened for carriage of class 1 and 2 integrons by PCR method. Results showed that seventy four (92.5%) of 80 MDRAB carried class 1 integron. Integron-positive isolates were statistically more resistant to aminoglycoside, quinolone and beta-lactam compounds except for cefepime. This is the first report of class 1 integrons in MDRAB isolates in northwest Iran.

scholarly journals A Plasmid-Encoded Class 1 Integron Contains GES-Type Extended-Spectrum β-Lactamases in Enterobacteriaceae Clinical Isolates in Mexico

2012 ◽  
Vol 56 (7) ◽  
pp. 4032-4034 ◽  
Author(s):  
Humberto Barrios ◽  
Ulises Garza-Ramos ◽  
Luz Edith Ochoa-Sanchez ◽  
Fernando Reyna-Flores ◽  
Teresa Rojas-Moreno ◽  
...  
Keyword(s):  
Clinical Isolates ◽  
Class 1 Integron ◽  
Extended Spectrum ◽  
Class 1

scholarly journals Emergence of Multidrug-Resistant Klebsiella pneumoniae Isolates Producing VIM-4 Metallo-β-Lactamase, CTX-M-15 Extended-Spectrum β-Lactamase, and CMY-4 AmpC β-Lactamase in a Tunisian University Hospital

2006 ◽  
Vol 50 (12) ◽  
pp. 4198-4201 ◽  
Author(s):  
Sonia Ktari ◽  
Guillaume Arlet ◽  
Basma Mnif ◽  
Valérie Gautier ◽  
Fouzia Mahjoubi ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Gel Electrophoresis ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Isolates ◽  
University Hospital ◽  
Class 1 Integron ◽  
Pulsed Field ◽  
Extended Spectrum ◽  
Class 1

ABSTRACT Klebsiella pneumoniae clinical isolates resistant to carbapenems were recovered from 11 patients in the hospital of Sfax, Tunisia. The isolates were closely related as shown by pulsed-field gel electrophoresis, and they produced VIM-4 metallo-enzyme, CTX-M-15 extended-spectrum β-lactamase, and CMY-4 AmpC enzyme. The bla VIM-4 gene is part of a class 1 integron.

scholarly journals qnrA in CTX-M-Producing Escherichia coli Isolates from France

2006 ◽  
Vol 50 (12) ◽  
pp. 4224-4228 ◽  
Author(s):  
Jean-Philippe Lavigne ◽  
Hélène Marchandin ◽  
Julien Delmas ◽  
Nicole Bouziges ◽  
Evelyne Lecaillon ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Clinical Isolates ◽  
Class 1 Integron ◽  
E Coli ◽  
Extended Spectrum ◽  
Class 1 ◽  
First Time

ABSTRACT By PCR, we screened for qnr genes 112 clinical isolates of extended-spectrum β-lactamase-producing Escherichia coli collected from hospitals in France during 2004. For the first time, 7.7% of CTX-M-producing E. coli isolates presented a plasmid-mediated resistance to quinolones. All strains harbored a qnrA gene located on a sul1-type class 1 integron with similar structure to the In36 integron.

scholarly journals Prevalence of extended spectrum beta lactamase and plasmid mediated quinolone resistant genes in strains of Klebsiella pneumonia, Morganella morganii, Leclercia adecarboxylata and Citrobacter freundii isolated from poultry in South Western Nigeria

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5053 ◽  
Author(s):  
Olajumoke R. Akinbami ◽  
Samson Olofinsae ◽  
Funmilola A. Ayeni
Keyword(s):  
Resistance Genes ◽  
Animal Husbandry ◽  
Quinolone Resistance ◽  
Klebsiella Pneumonia ◽  
Citrobacter Freundii ◽  
Beta Lactamase ◽  
Beta Lactam ◽  
Morganella Morganii ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum

A serious concern is arising on the coexistence of extended-spectrum beta-lactamase (ESBL) and plasmid mediated quinolone resistance (PMQR) producing bacteria in animal husbandry, which could be transferred to humans, especially in strains that may not be routinely screened for resistance. This study therefore tested the prevalence of ESBL and PMQR genes in selected bacteria isolated from poultry faeces. Faecal droppings of birds were collected from 11 farms in five states in South Western Nigeria. Bacteria were isolated from the samples on cefotaxime supplemented plates and identified with MALDI-TOF. The MIC was determined using VITEK system and resistance genes were detected with PCR. A total of 350 strains were isolated from different samples and selected strains were identified as 23 Klebsiella pneumonia, 12 Morganella morganii, seven Leclercia adecarboxylata and one Citrobacter freundii. All the species were resistant to gentamycin, trimethoprim/sulphamethaxole, tobramycin, piperacillin, cefotaxime and aztreonam (except Morganella morganii strains which were mostly susceptible to aztreonam). All the tested strains were susceptible to imipenem, meropenem and amikacin. All Leclercia adecarboxylata strains were resistant to ceftazidime, cefepime and fosfomycin while all Morganella morganii strains were resistant to fosfomycin, moxifloxacin and ciprofloxacin. All tested species were generally sensitive to ciprofloxacin except Morganella morganii strains which were resistant to ciprofloxacin. The resistance to ciprofloxacin, ceftazidime, cefepime, tigercylin, colistin and fosfomycin were 65%, 40%, 23%,, 7%, 33%, 48% respectively while the prevalence of SHV, TEM and CTX genes were 42%, 63%, 35% respectively. 9.3% of the isolates had the three ESBL genes, 2.33% had qnrA gene, 4.65% had qnr B gene while none had qnrS gene. The most prevalent PMQR gene is Oqxb (25.58%) while 6.98% had the qep gene. Klebsiella pneumoniae generally had both ESBL and PMQR genes. The high prevalence of extended spectrum beta-lactamase genes in the studied strains calls for caution in the use of beta lactam antibiotics in poultry feeds. This is the first report of the occurrence of extended spectrum beta-lactamase and plasmid mediated quinolone resistance genes in Morganella morganii and Leclercia adecarboxylata strains isolated from poultry faeces.

scholarly journals High prevalence of plasmid-mediated quinolone resistance (PMQR) among E. coli from aquatic environments in Bangladesh

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0261970
Author(s):  
Mohammed Badrul Amin ◽  
Sumita Rani Saha ◽  
Md Rayhanul Islam ◽  
S. M. Arefeen Haider ◽  
Muhammed Iqbal Hossain ◽  
...  
Keyword(s):  
Water Samples ◽  
Point Mutations ◽  
Quinolone Resistance ◽  
Multi Drug Resistance ◽  
Aquatic Environments ◽  
Cellulose Membrane ◽  
Biochemical Tests ◽  
Class 1 Integron ◽  
Extended Spectrum ◽  
Class 1

Fluro(quinolones) is an important class of antibiotic used widely in both human and veterinary medicine. Resistance to fluro(quinolones) can be acquired by either chromosomal point mutations or plasmid-mediated quinolone resistance (PMQR). There is a lack of studies on the prevalence of PMQR in organisms from environmental sources in Bangladesh. In this study, we investigated the occurrence of PMQR genes in E. coli from various water sources and analysed associations between multi-drug resistance (MDR) and resistance to extended spectrum β-lactam antibiotics. We analysed 300 E. coli isolates from wastewaters of urban live-bird markets (n = 74) and rural households (n = 80), rural ponds (n = 71) and river water samples (n = 75) during 2017–2018. We isolated E. coli by filtering 100 ml of water samples through a 0.2μm cellulose membrane and incubating on mTEC agar media followed by identification of isolated colonies using biochemical tests. We selected one isolate per sample for detection of PMQR genes by multiplex PCR and tested for antibiotic susceptibility by disc diffusion. Clonal relatedness of PMQR-positive isolates was evaluated by enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR). About 66% (n = 199) of E. coli isolates harbored PMQR-genes, predominantly qnrS (82%, n = 164) followed by aac(6’)-lb-cr (9%, n = 17), oqxAB (7%, n = 13), qnrB (6%, n = 11) and qepA (4%, n = 8). Around 68% (n = 135) of PMQR-positive isolates were MDR and 92% (n = 183) were extended spectrum β-lactamase (ESBL)-producing of which the proportion of positive samples was 87% (n = 159) for blaCTX-M-1’ 34% (n = 62) for blaTEM, 9% (n = 16) for blaOXA-1, blaOXA-47 and blaCMY-2, and 2% (n = 4) for blaSHV. Further, 16% (n = 32) of PMQR-positive isolates were resistant to carbapenems of which 20 isolates carried blaNDM-1. Class 1 integron (int1) was found in 36% (n = 72) of PMQR-positive E. coli isolates. PMQR genes were significantly associated with ESBL phenotypes (p≤0.001). The presence of several PMQR genes were positively associated with ESBL and carbapenemase encoding genes such as qnrS with blaCTXM-1 (p<0.001), qnrB with blaTEM (p<0.001) and blaOXA-1 (p = 0.005), oqxAB and aac(6’)-lb-cr with blaSHV and blaOXA-1 (p<0.001), qnrB with blaNDM-1 (p<0.001), aac(6’)-lb-cr with blaOXA-47 (p<0.001) and blaNDM-1 (p = 0.002). Further, int1 was found to correlate with qnrB (p<0.001) and qepA (p = 0.011). ERIC-PCR profiles allowed identification of 84 of 199 isolates with 85% matching profiles which were further grouped into 33 clusters. Only 5 clusters had isolates (n = 11) with identical ERIC-PCR profiles suggesting that PMQR-positive E. coli isolates are genetically heterogeneous. Overall, PMQR-positive MDR E. coli were widely distributed in aquatic environments of Bangladesh indicating poor wastewater treatment and highlighting the risk of transmission to humans and animals.

scholarly journals Incidence and Characterization of Integrons, Genetic Elements Mediating Multiple-Drug Resistance, in AvianEscherichia coli

1999 ◽  
Vol 43 (12) ◽  
pp. 2925-2929 ◽  
Author(s):  
Lydia Bass ◽  
Cynthia A. Liebert ◽  
Margie D. Lee ◽  
Anne O. Summers ◽  
David G. White ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Multiple Drug Resistance ◽  
Marker Gene ◽  
Clinical Isolates ◽  
Multiple Antibiotic Resistance ◽  
Class 1 Integron ◽  
E Coli ◽  
Class 1

ABSTRACT Antibiotic resistance among avian bacterial isolates is common and is of great concern to the poultry industry. Approximately 36% (n = 100) of avian, pathogenic Escherichia coli isolates obtained from diseased poultry exhibited multiple-antibiotic resistance to tetracycline, oxytetracycline, streptomycin, sulfonamides, and gentamicin. Clinical avian E. coli isolates were further screened for the presence of markers for class 1 integrons, the integron recombinase intI1 and the quaternary ammonium resistance gene qacEΔ1, in order to determine the contribution of integrons to the observed multiple-antibiotic resistance phenotypes. Sixty-three percent of the clinical isolates were positive for the class 1 integron markersintI1 and qacEΔ1. PCR analysis with the conserved class 1 integron primers yielded amplicons of approximately 1 kb from E. coli isolates positive for intI1 andqacEΔ1. These PCR amplicons contained the spectinomycin-streptomycin resistance gene aadA1. Further characterization of the identified integrons revealed that many were part of the transposon Tn21, a genetic element that encodes both antibiotic resistance and heavy-metal resistance to mercuric compounds. Fifty percent of the clinical isolates positive for the integron marker gene intI1 as well as for theqacEΔ1 and aadA1 cassettes also contained the mercury reductase gene merA. The correlation between the presence of the merA gene with that of the integrase and antibiotic resistance genes suggests that these integrons are located in Tn21. The presence of these elements among avianE. coli isolates of diverse genetic makeup as well as inSalmonella suggests the mobility of Tn21 among pathogens in humans as well as poultry.

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