An imbalance in the production of proinflammatory and anti-inflammatory cytokines is observed in whole blood cultures of preeclamptic women in comparison with healthy pregnant women

2014 ◽  
Vol 33 (2) ◽  
pp. 236-249 ◽  
Author(s):  
Jorge Campos-Cañas ◽  
Inés Romo-Palafox ◽  
Mario Albani-Campanario ◽  
César Hernández-Guerrero
Keyword(s):  
Pregnant Women ◽  
Inflammatory Cytokines ◽  
Whole Blood ◽  
Blood Cultures ◽  
Anti Inflammatory

scholarly journals СONCENTRATION OF ANTI-INFLAMATORY CYTOKINES IN CELL CULTURE SUPERNATANTS IN CHILDREN WITH JUVENILE IDIOPATHIC ARTHRITIS

2019 ◽  
Vol 21 (4) ◽  
pp. 725-736
Author(s):  
I. M. Krivolapova ◽  
I. A. Pashnina ◽  
V. A. Chereshnev
Keyword(s):  
Juvenile Idiopathic Arthritis ◽  
Inflammatory Cytokines ◽  
Whole Blood ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Blood Cultures ◽  
Juvenile Arthritis ◽  
Peripheral Blood Cells ◽  
Healthy Children ◽  
Anti Inflammatory

Juvenile idiopathic arthritis is a chronic inflammatory disease of the joints in children, mainly of autoimmune or auto-inflammatory nature. It is a heterogeneous group, which includes different subtypes of the disease. Different mechanisms may play role in the pathogenesis of distinct subtypes of juvenile arthritis. However, a long-term imbalance of pro- and anti-inflammatory cytokines is important for all subtypes of disease. The aim of the present study was to determine spontaneous and stimulated anti-inflammatory cytokines production by peripheral blood cells from the children with juvenile idiopathic arthritis. Patients of 2 to 17 years old with different subtypes of juvenile idiopathic arthritis (n = 99) and healthy children without signs of autoimmune diseases (control, n = 31) were examined. Spontaneous and phytohemagglutinin-stimulated concentrations of IL-1ra, IL-4, IL-10, TGF-β in supernatants of whole-blood cultures were determined by ELISA. Differences in the spontaneous and mitogen-stimulated secretion of the cytokines in patients with different subtypes of juvenile arthritis have not been revealed. The spontaneous IL-1ra, IL-4 and IL-10 production by blood cells in the common group of patients with juvenile idiopathic arthritis was similar to the controls. The median value of spontaneous TGF-β concentration in the patients was below the detection level, whereas blood cells of healthy children had a higher potential of spontaneous TGF-β production. IL-4 and IL-10 production after incubation of peripheral blood cells with phytohemagglutinin in patients and in the control group did not differ from the controls, while IL-1ra and TGF-β synthesis was significantly lower than in healthy children.The spontaneous and/or stimulated production of IL-1ra, TGF-β by blood cells in children with juvenile idiopathic arthritis reflects the pathogenic significance of these cytokines in disease. Stimulation of cells can reveal a latent deficiency in the synthesis of cytokines, which is not evident when determining its concentration in serum or supernatants of spontaneous whole-blood cultures.

The production of anti-inflammatory cytokines in whole blood by physico-chemical induction

2003 ◽  
Vol 52 (10) ◽  
pp. 404-407 ◽  
Author(s):  
H. Meijer ◽  
J. Reinecke ◽  
C. Becker ◽  
G. Tholen ◽  
P. Wehling
Keyword(s):  
Inflammatory Cytokines ◽  
Whole Blood ◽  
Chemical Induction ◽  
Physico Chemical ◽  
Anti Inflammatory

scholarly journals Plasma levels of melatonin, certain cytokines and placental growth factor at non-pharmacological correction of pineal function in pregnant women with intrauterine growth restriction

2020 ◽  
Vol 8 (2) ◽  
Author(s):  
A. Berbets ◽  
Keyword(s):  
Immune System ◽  
Growth Factor ◽  
Pineal Gland ◽  
Pregnant Women ◽  
Inflammatory Cytokines ◽  
Proinflammatory Cytokines ◽  
Placental Growth Factor ◽  
Placental Insufficiency ◽  
Tnf Α ◽  
Anti Inflammatory

The pineal gland produces the important hormone melatonin, the level of which in the blood of pregnant women decreases in case of placental insufficiency. The effect of dysfunction of the pineal gland on the immune system of pregnant women and on the angiogenic activity of the placenta during pregnancy remains insufficiently studied. Objective: to establish the effect of our method of non-drug correction of function of pineal gland on the state of the cytokine part of the immune system and on the synthesis of placental growth factor (PlGF) in pregnant women with placental insufficiency manifesting as fetal intrauterine growth restriction (IUGR). Material and methods. 46 pregnant women with IUGR at 30-36 weeks of gestation were examined. The group was divided into two subgroups: with non-drug correction of the pineal gland function (n = 25) and without correction (n = 21). The method of correction included a set of measures of following of lighting regimen, activity and sleep for 14 days. The control group consisted of 20 women with uncomplicated pregnancy. Levels of melatonin, PlGF, TNF-α, IL-1β, IL-6, IL-4, IL-10 were determined in the venous blood by enzyme-linked immunosorbent assay. Results. It was established that the concentration of melatonin in the blood of pregnant women with IUGR was significantly reduced, as well as the concentration of PlGF (p < 0.01). Significant changes were also found in pregnant women with placental insufficiency, namely, increased concentrations of proinflammatory cytokines, such as TNF-α (p < 0.05), IL-1-β (p < 0.001) and IL-6 (p < 0.05), comparing to healthy pregnant women. Also, in the group of pregnant women with IUGR the levels of anti-inflammatory cytokines IL-4 (p <0.001) and IL-10 (p < 0.001) were elevated in comparison to the control group. After application of the developed complex of non-drug correction of pineal gland function, the concentration of melatonin in the blood of pregnant women in the subgroup of correction increased significantly, comparing to the subgroup without correction (p < 0.001), as well as the level of PlGF (p < 0.05). Also, significantly lower levels of proinflammatory cytokines TNF-α, IL-1-β and IL-6 were observed in pregnant women in the subgroup of correction (p < 0.01). Regarding anti-inflammatory cytokines, under the influence of the developed complex of measures there was a decrease in the level of IL-4 and an increase in the level of IL-10 (p < 0.01). Conclusions. When the measures, aimed at non-drug correction of function of pineal gland, are applied in pregnant women with placental insufficiency, manifested as IUGR, the following changes are observed: increased of plasma levels of melatonin and placental growth factor, decreased of levels of proinflammatory cytokines. We suggest that the pineal gland exerts its effect on the immune system through melatonin, which moderates the activity of pro- and anti-inflammatory cytokines, thereby reducing the influence of inflammation on placental tissue, what results in increasing of concentrations of placental growth factor in the blood of pregnant women.

Levels of pro-inflammatory and anti-inflammatory cytokines and interferons in pregnant women with vaginal infections during vitamin D therapy

2019 ◽  
Vol 9_2019 ◽  
pp. 75-81
Author(s):  
Kostinov M.P. Kostinov ◽  
Ignat’eva M.A. Ignat’eva ◽  
Novikova S.V. Novikova ◽  
Shmit’ko A.D. Shmit’ko ◽  
Polishchuk V.B. Polishchuk ◽  
...  
Keyword(s):  
Vitamin D ◽  
Pregnant Women ◽  
Inflammatory Cytokines ◽  
Vaginal Infections ◽  
Anti Inflammatory

scholarly journals Physiologically relevant aspirin concentrations trigger immunostimulatory cytokine production by human leukocytes

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0254606
Author(s):  
Regine Brox ◽  
Holger Hackstein
Keyword(s):  
Acetylsalicylic Acid ◽  
Inflammatory Cytokines ◽  
Whole Blood ◽  
Plasma Concentrations ◽  
Immunomodulatory Activity ◽  
Prostaglandin E ◽  
Dependent Manner ◽  
Cox 2 ◽  
Anti Inflammatory

Acetylsalicylic acid is a globally used non-steroidal anti-inflammatory drug (NSAID) with diverse pharmacological properties, although its mechanism of immune regulation during inflammation (especially at in vivo relevant doses) remains largely speculative. Given the increase in clinical perspective of Acetylsalicylic acid in various diseases and cancer prevention, this study aimed to investigate the immunomodulatory role of physiological Acetylsalicylic acid concentrations (0.005, 0.02 and 0.2 mg/ml) in a human whole blood of infection-induced inflammation. We describe a simple, highly reliable whole blood assay using an array of toll-like receptor (TLR) ligands 1–9 in order to systematically explore the immunomodulatory activity of Acetylsalicylic acid plasma concentrations in physiologically relevant conditions. Release of inflammatory cytokines and production of prostaglandin E2 (PGE2) were determined directly in plasma supernatant. Experiments demonstrate for the first time that plasma concentrations of Acetylsalicylic acid significantly increased TLR ligand-triggered IL-1β, IL-10, and IL-6 production in a dose-dependent manner. In contrast, indomethacin did not exhibit this capacity, whereas cyclooxygenase (COX)-2 selective NSAID, celecoxib, induced a similar pattern like Acetylsalicylic acid, suggesting a possible relevance of COX-2. Accordingly, we found that exogenous addition of COX downstream product, PGE2, attenuates the TLR ligand-mediated cytokine secretion by augmenting production of anti-inflammatory cytokines and inhibiting release of pro-inflammatory cytokines. Low PGE2 levels were at least involved in the enhanced IL-1β production by Acetylsalicylic acid.

scholarly journals Fc-gamma receptor expression and cytokine response to intravenous human immunoglobulin in mothers and neonates

2021 ◽  
Author(s):  
Stephania Vazquez-Rodriguez ◽  
Lourdes A. Arriaga-Pizano ◽  
Ismael Mancilla-Herrera ◽  
Jessica Prieto-Chávez ◽  
Roberto Arizmendi-Villanueva ◽  
...  
Keyword(s):  
Adjuvant Therapy ◽  
Inflammatory Cytokines ◽  
Whole Blood ◽  
Blood Cultures ◽  
Receptor Expression ◽  
Human Immunoglobulin ◽  
List Type ◽  
Fc Gamma Receptor ◽  
Gamma Receptor ◽  
Pro Inflammatory Cytokines

AbstractObjectiveThe use of intravenous human immunoglobulin (IVIg) as adjuvant therapy for sepsis has been shown efficacious in adults, but its use in pregnant women and newborns is controversial. Fc gamma receptors (FcγRs) support the ability of IVIg to stimulate the synthesis of inflammatory mediators and promote phagocytosis by leukocytes, however, the FcγRs expression is differential between adults and neonates. We aimed to explore the effect of IVIg in monocytes and neutrophils from mother and neonates in whole blood cultures.Study designWhole blood from adults, maternal, and neonates were incubated with LPS and/or IVIg. After 0, 24, and 48 hours, we measured the expression of FcγRs (CD16, CD32, and CD64) and bacterial phagocytosis by monocytes and neutrophils. Also, the concentration of pro-inflammatory cytokines/chemokines was determined.ResultsFcγRs expression is quite similar among groups, and the LPS or IVIg challenge did not change the FcγRs expression on monocytes and neutrophils. Also, the LPS or IVIg challenge did not modify phagocytosis capacity in any group. However, IVIg induces a higher IL-8 response in neonates than in adults.ConclusionOur results suggest that the IL-8 response to IVIg in whole blood from neonates is not dependent on differential FcγR expression.Key messagesIVIg challenge in neonates or adults does not induce FcγR change expression on monocytes or neutrophilsIVIg induces higher IL-8 response in neonates than in adults

scholarly journals Acute high-intensity interval exercise reduces human monocyte Toll-like receptor 2 expression in type 2 diabetes

2017 ◽  
Vol 312 (4) ◽  
pp. R529-R538 ◽  
Author(s):  
Cody Durrer ◽  
Monique Francois ◽  
Helena Neudorf ◽  
Jonathan P. Little
Keyword(s):  
Type 2 Diabetes ◽  
Whole Blood ◽  
Blood Cultures ◽  
Toll Like Receptor ◽  
Single Session ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
High Intensity Interval ◽  
The Impact

Type 2 diabetes (T2D) is characterized by chronic low-grade inflammation that contributes to disease pathophysiology. Exercise has anti-inflammatory effects, but the impact of high-intensity interval training (HIIT) is not known. The purpose of this study was to determine the impact of a single session of HIIT on cellular, molecular, and circulating markers of inflammation in individuals with T2D. Participants with T2D ( n = 10) and healthy age-matched controls (HC; n = 9) completed an acute bout of HIIT (7 × 1 min at ~85% maximal aerobic power output, separated by 1 min of recovery) on a cycle ergometer with blood samples obtained before (Pre), immediately after (Post), and at 1 h of recovery (1-h Post). Inflammatory markers on leukocytes were measured by flow cytometry, and TNF-α was assessed in both LPS-stimulated whole blood cultures and plasma. A single session of HIIT had an overall anti-inflammatory effect, as evidenced by 1) significantly lower levels of Toll-like receptor (TLR) 2 surface protein expression on both classical and CD16+ monocytes assessed at Post and 1-h Post compared with Pre ( P < 0.05 for all); 2) significantly lower LPS-stimulated TNF-α release in whole blood cultures at 1-h Post ( P < 0.05 vs. Pre); and 3) significantly lower levels of plasma TNF-α at 1-h Post ( P < 0.05 vs. Pre). There were no differences between T2D and HC, except for a larger decrease in plasma TNF-α in HC vs. T2D (group × time interaction, P < 0.05). One session of low-volume HIIT has immunomodulatory effects and provides potential anti-inflammatory benefits to people with, and without, T2D.

Sign in / Sign up

Export Citation Format

Share Document