scholarly journals Novel Pathway of Heat Shock- Induced Resistance in Tomato

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Tatsuo Sato ◽  
Nur Akbar Arofatullah ◽  
Sayuri Tanabata
Keyword(s):  
Salicylic Acid ◽  
Disease Resistance ◽  
Heat Shock ◽  
Induced Resistance ◽  
Time Lag ◽  
Temperature Treatment ◽  
High Temperature Treatment ◽  
Pathogenesis Related Protein ◽  
Salicylic Acid Accumulation ◽  
Acid Pathway

High-temperature treatment induces disease resistance in various plants (heat shock-induced resistance; HSIR). The role of heat shock transcriptional factors (Hsfs) was investigated in this paper. Heat shock treatment induced disease resistance and up-regulate gene expression of pathogenesis related protein; PR1a2 at 12 and 24 h after treatment. PR1a2 has putative Hsfs binding site in the upstream area. On the other hand, a heat shock transcription factor HsfA2 up-regulated at 6 h after treatment, which was 6 h earlier than salicylic acid accumulation. This time lag suggested the direct contribution of Hsfs, additionally to salicylic acid pathway in the regulation of HSIR in tomato.

scholarly journals Expression of Target Gene Hsp70 and Membrane Stability Determine Heat Tolerance in Chili Pepper

2015 ◽  
Vol 140 (2) ◽  
pp. 144-150 ◽  
Author(s):  
Magaji G. Usman ◽  
Mohd Y. Rafii ◽  
Mohd Razi Ismail ◽  
Mohammad Abdul Malek ◽  
Mohammad Abdul Latif
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Heat Tolerance ◽  
Membrane Integrity ◽  
Temperature Treatment ◽  
Chili Pepper ◽  
High Temperature Treatment ◽  
Experimental Plant ◽  
Membrane Thermostability ◽  
Heat Tolerant

Experiments were carried out to study the mechanisms for heat tolerance in chili pepper (Capsicum annuum). To assess these mechanisms, six genotypes were evaluated for cellular membrane thermostability (CMT) and for HSP70 gene expression. The plants were grown in an experimental plant growth chamber. The mean value of CMT indicates that membrane integrity was not damaged by the high temperature treatment (50 °C) in most of the genotypes. The genotypes were classified as follows: heat-tolerant (greater than 60%), moderately tolerant (30% to 60%), and susceptible (less than 30%). The heat-tolerant plants recorded the highest CMTs at 89.27%, 88.03%, and 85.10% for AVPP0702, AVPP0116, and AVPP9905, respectively, which might be the reason for the change in their cell membrane thermostability. AVPP9703 and AVPP0002 showed CMTs of 15.87% and 18.43%, which might indicate their sensitivity to heat stress. Heat shock protein 70 kDa was identified and found to be differentially expressed under the heat stress. Under heat stress, significantly increased levels of the HSP70 gene were detected after 2 h of temperature treatment at 42 °C, which indicated that this gene is quickly and sharply induced by heat shock. This was true for all genotypes tested, which were significantly up-regulated by more than 36.9-, 7.10-, 3.87-, and 3-fold for AVPP0702, AVPP0116, AVPP0002, and AVPP9703, respectively. The HSP70 gene was found to be significantly down-regulated under heat stress in ‘Kulai’. AVPP0702, AVPP9905, and AVPP0116 could be considered as heat-tolerant genotypes, whereas ‘Kulai’ and AVPP9703 were found to be heat-sensitive genotypes in this investigation.

scholarly journals Domains of the cucumber mosaic virus 2b silencing suppressor protein affecting inhibition of salicylic acid-induced resistance and priming of salicylic acid accumulation during infection

2014 ◽  
Vol 95 (6) ◽  
pp. 1408-1413 ◽  
Author(s):  
Tao Zhou ◽  
Alex M. Murphy ◽  
Mathew G. Lewsey ◽  
Jack H. Westwood ◽  
Heng-Mu Zhang ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Induced Resistance ◽  
Marker Gene ◽  
Silencing Suppressor ◽  
Salicylic Acid Accumulation ◽  
2B Protein ◽  
Local Movement ◽  
Phosphorylation Domain

The cucumber mosaic virus (CMV) 2b silencing suppressor protein allows the virus to overcome resistance to replication and local movement in inoculated leaves of plants treated with salicylic acid (SA), a resistance-inducing plant hormone. In Arabidopsis thaliana plants systemically infected with CMV, the 2b protein also primes the induction of SA biosynthesis during this compatible interaction. We found that CMV infection of susceptible tobacco (Nicotiana tabacum) also induced SA accumulation. Utilization of mutant 2b proteins expressed during infection of tobacco showed that the N- and C-terminal domains, which had previously been implicated in regulation of symptom induction, were both required for subversion of SA-induced resistance, while all mutants tested except those affecting the putative phosphorylation domain had lost the ability to prime SA accumulation and expression of the SA-induced marker gene PR-1.

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