Heat shock-induced resistance increases chitinase-1 gene expression and stimulates salicylic acid production in melon (Cucumis melo L.)

*Abstract: We investigated the role of heat shock transcription factors (Hsfs) during induction of defense response by heat-shock treatment (HST) in tomato. Leaf disease symptoms were significantly reduced at 12 and 24 h after HST, consistent with upregulation of pathogenesis-related (PR) genes PR1a2 and PR1b1 peaking at 24 h after treatment. These genes were upregulated at the treatment application site, but not in untreated leaves. In contrast to HST, inoculation of the first leaf induced systemic upregulation of acidic PR genes in uninoculated second leaves. Furthermore, heat shock element motifs were found in upstream regions of PR1a2, PR1b1, Chitinase 3, Chitinase 9, Glucanase A, and Glucanase B genes. Upregulation of HsfA2 and HsfB1 peaked at 6 h after HST, 6 h earlier than salicylic acid accumulation. Foliar spray of heat shock protein 90 (Hsp90) inhibitor geldanamycin (GDA) induced PR gene expression comparable to that after HST. PR gene expression and defense response against Pseudomonas syringae pv. tomato (Pst) decreased when combining HST with Hsfs inhibitor KRIBB11. The Hsfs and PR gene expression induced by heat or GDA, together with the suppression of heat shock-induced resistance (HSIR) against Pst by KRIBB11, suggested a direct contribution of Hsfs to HSIR regulation in tomato.

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Sodium Silicate Reduces Postharvest Decay on Hami Melons: Induced Resistance and Fungistatic Effects

Plant Disease ◽

10.1094/pd-90-0279 ◽

2006 ◽

Vol 90 (3) ◽

pp. 279-283 ◽

Cited By ~ 63

Author(s):

Y. Bi ◽

S. P. Tian ◽

Y. R. Guo ◽

Y. H. Ge ◽

G. Z. Qin

Keyword(s):

Sodium Silicate ◽

Induced Resistance ◽

Alternaria Alternata ◽

Cucumis Melo ◽

Mycelial Growth ◽

Room Temperature ◽

Post Inoculation ◽

Fusarium Semitectum ◽

Cucumis Melo L

The effect of sodium silicate (Si) for control of decay was tested in Hami melons (Cucumis melo L. var. inodorus Jacq.). Si significantly inhibited mycelial growth of Alternaria alternata, Fusarium semitectum, and Trichothecium roseum in vitro. Si at 100 mM was more effective than Si at 25 or 50 mM at controlling the diseases caused by the three pathogens, whereas Si at 200 mM was phytotoxic. Si treatments applied at 100 mM pre-inoculation with T. roseum had lower decay incidence and severity than treatments applied post-inoculation. The protection of Si was correlated with the activation of two families of defense-related enzymes, peroxidase and chitinase. Accumulation of both enzymes was induced in fruit treated with Si and challenged by T. roseum 24 h later, and was sustained for at least 9 days in ‘New Queen’ and 10 days in ‘8601’ at room temperature. It appeared that induced resistance was an important mechanism of disease control in Hami melons treated with Si.

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CmBGI Gene Expression encoding β-glucosidase in melon (Cucumis melo L.) under stress condition

BIOTROPIC The Journal of Tropical Biology ◽

10.29080/biotropic.2017.1.2.1-8 ◽

2017 ◽

Vol 1 (2) ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Yuanita Rachmawati ◽

Ganies Rizaa Aristya ◽

Budi Setiadi Daryono

Keyword(s):

Gene Expression ◽

Normal Condition ◽

Cucumis Melo ◽

Stress Condition ◽

Leaf Tissue ◽

Adaptive Responses ◽

Degraded Land ◽

Biotic Stresses ◽

Aba Metabolism ◽

Cucumis Melo L

CmBGI is the enzymatic genes encoding β-glucosidase that involved in Abscisic Acid (ABA) metabolism of Cucumis melo L. β-glucosidase promotes the accumulation of glucose, fructose, and sucrose, and it might act as a regulator that mediates melon fruit ripening both climacteric and nonclimacteric. ABA mediates adaptive responses to abiotic and biotic stresses. Agricultural Balitbang in 1997 showed that there were approximately 158.600 ha of degraded land scattered in three zones of agroecosystems in Yogyakarta (DIY). One of them is Dlingo Bantul area which has a karst type critical land area. Karst provides stress to the certain plant growth. One way to conserve critical land is making this area for agriculture. Cultivar TACAPA and TA were superior melons that have been developed by Genetic Laboratory of Biology Faculty UGM. This preliminary research was conducted to examine molecular characterization of CmBGI gene expression in cultivar TACAPA and TA which are planted in normal condition medium and in critical land medium treatment. Total RNA was extracted from leaf tissue then Reversed Transcriptase (RT-PCR) to collect cDNA library. cDNA was amplified using specific primer. Spectrophotometry was conducted in λ260 nm and electrophoresis run in 1.5% agarose gel. Control of band chosen was Cm-Actin. CmBGI gene concentration of TACAPA and TA in normal condition medium are in succession 578.5 and 579.4 μg/ml then for critical land medium treatment 743.4 and 773.5 μg/ml. CmBGI band was showed both of TACAPA and TA as ± 1258 bp. Cm-actin was showed band of DNA as ± 445 bp. CmBGI gene concentration in critical land medium treatment which is given greater stress on melons are higher than normal condition. This suggests that the CmBGI gene is expressed more in cultivar TACAPA and TA melons when they are grown under stress condition.

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Stimulation of benzyladenine-induced in vitro shoot organogenesis in Cucumis melo L. by proline, salicylic acid and aspirin

Plant Science ◽

10.1016/0168-9452(92)90134-8 ◽

1992 ◽

Vol 84 (2) ◽

pp. 193-199 ◽

Cited By ~ 20

Author(s):

Kalidas Shetty ◽

Geetha A. Shetty ◽

Yukoh Nakazaki ◽

Keiko Yoshioka ◽

Yoshito Asano ◽

...

Keyword(s):

Salicylic Acid ◽

Cucumis Melo ◽

Shoot Organogenesis ◽

Cucumis Melo L ◽

Stimulation Of

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Induction of disease resistance against Botrytis cinerea by heat shock treatment in melon (Cucumis melo L.)

Physiological and Molecular Plant Pathology ◽

10.1016/j.pmpp.2011.04.002 ◽

2011 ◽

Vol 75 (4) ◽

pp. 157-162 ◽

Cited By ~ 13

Author(s):

Ani Widiastuti ◽

Mioko Yoshino ◽

Harufumi Saito ◽

Keisuke Maejima ◽

Songying Zhou ◽

...

Keyword(s):

Disease Resistance ◽

Heat Shock ◽

Botrytis Cinerea ◽

Cucumis Melo ◽

Shock Treatment ◽

Heat Shock Treatment ◽

Cucumis Melo L

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Effect of proline and salicylic acid on germination and antioxidant enzymes at different temperatures in Muskmelon (Cucumis melo L.) seeds

Journal of Applied and Natural Science ◽

10.31018/jans.v9i4.1504 ◽

2017 ◽

Vol 9 (4) ◽

pp. 2165-2169 ◽

Cited By ~ 1

Author(s):

Sandeep Kaur ◽

Namarta Gupta

Keyword(s):

Superoxide Dismutase ◽

Salicylic Acid ◽

Antioxidant Enzymes ◽

Cucumis Melo ◽

Seed Treatments ◽

Different Temperatures ◽

Percent Germination ◽

Anti Oxidant ◽

Lower Temperature ◽

Cucumis Melo L

Effect of different seed treatments hydration, warm water, proline (10mM and 20mM) and salicylic acid (0.1mM and 0.5mM) was investigated on percent germination and activity of antioxidant enzymes viz. superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) at different temperatures in Muskmelon (Cucumis melo L.) seeds. It was observed that lower temperature (20˚C) had decreased seed germination and activity of various anti-oxidant enzymes. Various seed treatments increased percent germination and activity of these enzymes as com-pared to control at both the temperatures. Proline 20mM (96.6) and SA 0.1mM (91.6) showed better results as com-pared to proline 10mM (95.0) and salicylic acid 0.5mM (86.6) respectively. Thus, proline and salicylic acid play an ameliorating role on low temperature stress by enhancing the activities of antioxidant enzymes and scavenging the free radicals.

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