scholarly journals Viv: Multiscale Visualization of High-Resolution Multiplexed Bioimaging Data on the Web

2020 ◽  
Author(s):  
Trevor Manz ◽  
Ilan Gold ◽  
Nathan Heath Patterson ◽  
Chuck McCallum ◽  
Mark S Keller ◽  
...  

Recent advances in highly multiplexed imaging have enabled the comprehensive profiling of complex tissues in healthy and diseased states, facilitating the study of fundamental biology and human disease in spatially-resolved contexts at subcellular resolution. However, current computational infrastructure to distribute and visualize these data on the web remains complex to set up and maintain. To address these limitations, we have developed Viv—an open-source image visualization library for high-resolution multiplexed image data that is implemented in JavaScript and builds on modern web technologies. Viv directly renders Bio-Formats-compatible Zarr and OME-TIFF data formats. Three use cases, including integration into Jupyter Notebooks (https://github.com/hms-dbmi/vizarr) and a data portal, as well as an image viewer (http://avivator.gehlenborglab.org) demonstrate the capabilities of our proposed approach.

Author(s):  
Roger Hyam

Many of the world’s natural history collections are creating high resolution digital images of their specimens. They often make these available on the web through some form or zoomable viewer. For historical reasons, a hotchpotch of technologies are used to achieve this. This diversity has lead to two issues. Firstly, maintenance becomes costly as technologies need replacing. Secondly there is little chance to share data between institutions or provide a unified user experience. A researcher visiting four different virtual collections may have four very different experiences. Similar issues exist in the archives and libraries disciplines. They also need to share high resolution, annotated images of the physical objects in their care. In response to this issue many have coalesced around the International Image Interoperability Framework (IIIF). IIIF is a set of shared application programming interface (API) specifications for interoperable functionality in digital image repositories. It separates the notion of a viewer, which may be used as part of a website or other application, and the web services that feed data to that viewer. By using a common API for serving data about images, different viewers can be used to view the same images, thus providing an upgrade path that does not require replacement of viewer and server software at the same time and allows different viewers to be used for the same image data. Potentially more importantly, it facilitates the construction of applications that view data from different collections as if they were in the same place. From the researcher’s point of view, the experience could be the same whether the virtual specimen is hosted locally or in a museum on another continent. There is one important thing that has been deliberately omitted from the IIIF standard. This has both enabled its rapid adoption but also makes it incomplete for building research applications. IIIF transmits no semantic data about the subject of the images, only labels. The IIIF data therefore needs to be bound to semantically rich data about the specimens being viewed, in some uniform way. Consortium of Taxonomic Facilities (CETAF ) specimen identifiers are now widely adopted by natural history collections in Europe. Each individual collection object is designated by a URI chosen and maintained by the institution owning the specimen (Groom et al. 2017, Güntsch et al. 2018, Güntsch et al. 2017, HYAM et al. 2012). Under Linked Data conventions, content negotiation is used at the server so that users accessing an object using a web-browser are redirected to a human-readable representation of the object, typically a web-page, whilst software systems requiring machine-processable representations are redirected to an RDF-encoded metadata record. CETAF specimen identifiers are therefore ideal partners for IIIF representations of specimens. But how should we join the two together in a semantically rich way that will be generally understandable? SYNTHESYS+ is a European Commission funded programme that facilitates collaboration and network building among European natural history collections. It is concerned with both physical and virtual access to the 390 million specimens of plants and animals housed in participating institutions. Under Task 4.3 of this project, we have been working to create a reliable way to link between the RDF metadata about specimens and images of those specimens in IIIF as well as from images of specimens back to metadata of those specimens. By January 2021, we aim to have ten exemplar institutions publishing IIIF manifest files linked to CETAF identifiers for a few million specimens and for this to act as a catalyst for wider adoption in the natural history community. This presentation gives an update on the rollout of these implementations, paying particular attention to the challenges of semantically annotating specimens with images.


Fractals ◽  
2011 ◽  
Vol 19 (03) ◽  
pp. 347-354 ◽  
Author(s):  
CHING-JU CHEN ◽  
SHU-CHEN CHENG ◽  
Y. M. HUANG

This study discussed the application of a fractal interpolation method in satellite image data reconstruction. It used low-resolution images as the source data for fractal interpolation reconstruction. Using this approach, a high-resolution image can be reconstructed when there is only a low-resolution source image available. The results showed that the high-resolution image data from fractal interpolation can effectively enhance the sharpness of the border contours. Implementing fractal interpolation on an insufficient image resolution image can avoid jagged edges and mosaic when enlarging the image, as well as improve the visibility of object features in the region of interest. The proposed approach can thus be a useful tool in land classification by satellite images.


Author(s):  
David C. Joy ◽  
Dennis M. Maher

High-resolution images of the surface topography of solid specimens can be obtained using the low-loss technique of Wells. If the specimen is placed inside a lens of the condenser/objective type, then it has been shown that the lens itself can be used to collect and filter the low-loss electrons. Since the probeforming lenses in TEM instruments fitted with scanning attachments are of this type, low-loss imaging should be possible.High-resolution, low-loss images have been obtained in a JEOL JEM 100B fitted with a scanning attachment and a thermal, fieldemission gun. No modifications were made to the instrument, but a wedge-shaped, specimen holder was made to fit the side-entry, goniometer stage. Thus the specimen is oriented initially at a glancing angle of about 30° to the beam direction. The instrument is set up in the conventional manner for STEM operation with all the lenses, including the projector, excited.


2014 ◽  
Vol 31 (2) ◽  
Author(s):  
Mariela Gabioux ◽  
Vladimir Santos da Costa ◽  
Joao Marcos Azevedo Correia de Souza ◽  
Bruna Faria de Oliveira ◽  
Afonso De Moraes Paiva

Results of the basic model configuration of the REMO project, a Brazilian approach towards operational oceanography, are discussed. This configuration consists basically of a high-resolution eddy-resolving, 1/12 degree model for the Metarea V, nested in a medium-resolution eddy-permitting, 1/4 degree model of the Atlantic Ocean. These simulations performed with HYCOM model, aim for: a) creating a basic set-up for implementation of assimilation techniques leading to ocean prediction; b) the development of hydrodynamics bases for environmental studies; c) providing boundary conditions for regional domains with increased resolution. The 1/4 degree simulation was able to simulate realistic equatorial and south Atlantic large scale circulation, both the wind-driven and the thermohaline components. The high resolution simulation was able to generate mesoscale and represent well the variability pattern within the Metarea V domain. The BC mean transport values were well represented in the southwestern region (between Vitória-Trinidade sea mount and 29S), in contrast to higher latitudes (higher than 30S) where it was slightly underestimated. Important issues for the simulation of the South Atlantic with high resolution are discussed, like the ideal place for boundaries, improvements in the bathymetric representation and the control of bias SST, by the introducing of a small surface relaxation. In order to make a preliminary assessment of the model behavior when submitted to data assimilation, the Cooper & Haines (1996) method was used to extrapolate SSH anomalies fields to deeper layers every 7 days, with encouraging results.


2021 ◽  
Author(s):  
Jason R. Swedlow ◽  
Pasi Kankaanpää ◽  
Ugis Sarkans ◽  
Wojtek Goscinski ◽  
Graham Galloway ◽  
...  
Keyword(s):  

2018 ◽  
Vol 50 ◽  
pp. 02007
Author(s):  
Cecile Tondriaux ◽  
Anne Costard ◽  
Corinne Bertin ◽  
Sylvie Duthoit ◽  
Jérôme Hourdel ◽  
...  

In each winegrowing region, the winegrower tries to value its terroir and the oenologists do their best to produce the best wine. Thanks to new remote sensing techniques, it is possible to implement a segmentation of the vineyard according to the qualitative potential of the vine stocks and make the most of each terroir to improve wine quality. High resolution satellite images are processed in several spectral bands and algorithms set-up specifically for the Oenoview service allow to estimate vine vigour and a heterogeneity index that, used together, directly reflect the vineyard oenological potential. This service is used in different terroirs in France (Burgundy, Languedoc, Bordeaux, Anjou) and in other countries (Chile, Spain, Hungary and China). From this experience, we will show how remote sensing can help managing vine and wine production in all covered terroirs. Depending on the winegrowing region and its specificities, its use and results present some differences and similarities that we will highlight. We will give an overview of the method used, the advantage of implementing field intra-or inter-selection and how to optimize the use of amendment and sampling strategy as well as how to anticipate the whole vineyard management.


2016 ◽  
Vol 87 (7) ◽  
pp. 073702 ◽  
Author(s):  
H. von Allwörden ◽  
K. Ruschmeier ◽  
A. Köhler ◽  
T. Eelbo ◽  
A. Schwarz ◽  
...  

2016 ◽  
Vol 22 (6) ◽  
pp. 1316-1328 ◽  
Author(s):  
Michael Marko ◽  
Chyongere Hsieh ◽  
Eric Leith ◽  
David Mastronarde ◽  
Sohei Motoki

AbstractPhase plate (PP) imaging has proven to be valuable in transmission cryo electron microscopy of unstained, native-state biological specimens. Many PP types have been described, however until the recent implementation of the “hole-free” phase plate (HFPP), imaging has been challenging. We found the HFPP to be simple to construct and to set up in the transmission electron microscopy, but care in implementing automated data collection is needed. Performance may be variable, both initially and over time, thus it is important to monitor and evaluate image quality by observing the power spectrum. We found that while some HFPPs gave transfer to high resolution without CTF oscillation, most reached high resolution when operated with modest defocus.


2013 ◽  
Vol 411-414 ◽  
pp. 3035-3038
Author(s):  
Zhan Dong Li ◽  
Meng Li ◽  
Yan Liu

Taking the division of Gao3 reservoir group strata sequence in Songliao basin of Daqing Changyuan Xingbei for example, this paper analyzed the applicability of the one-dimensional continuous wavelet transform in high resolution sequence division. By means of making morlet wavelet transform on the natural gamma logging curve, the connection between well curve signal and depth is transformed to the alter relationship between depth and the domains of scale, which obtains the wavelet coefficient curve. Then set up the corresponding relations between periodic oscillations characteristics of wavelet coefficient curve and sequence interface at all levels under the optimal scale factor. And the wavelet coefficients curve in best scale factor will eventually be used for the identification and division of the sequence. Practice has proved that the wavelet coefficient curve in the best factor can accurately identify the sequence interface at all levels, and the result is more accurate, objective and effective than manual calibration. So take advantage of wavelet transform in sequence stratigraphic classification provides a new way for high resolution sequence stratigraphy studies.


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