scholarly journals Diferensiasi Leukosit Ikan Lele Dumbo (Clarias gariepinus) yang diberi Pakan Mengandung Kunyit (Curcuma domestica Val.) dan Diinfeksi Bakteri Aeromonas hydrophila

2021 ◽  
Vol 9 (2) ◽  
pp. 116
Author(s):  
Kiky Dirgantara Ginting ◽  
Morina Riauwaty ◽  
Henni Syawal
Keyword(s):  
Aeromonas Hydrophila ◽  
Clarias Gariepinus

Penelitian ini dilaksanakan pada bulan Agustus sampai Oktober 2020 di Laboratorium Parasit dan Penyakit Ikan, Jurusan Budidaya Perairan, Fakultas Perikanan dan Kelautan, Universitas Riau. Penelitian ini bertujuan untuk mendapatkan dosis terbaik pemberian pakan mengandung kunyit (Curcuma domestica Val.) pada ikan lele dumbo (Clarias gariepinus) untuk mencegah infeksi Aeromonas hydrophila. Metode yang digunakan adalah metode eksperimen dengan menggunakan Rancangan Acak Lengkap (RAL) satu faktor lima taraf perlakuan dan 3 kali ulangan. Perlakuan yang diterapkan adalah Kn (kontrol, tanpa kunyit), Kp (tanpa kunyit dan diuji tantang dengan A. hydrophila), P1 (0,5 g kunyit/kg pelet dan diuji tantang dengan A. hydrophila), P2 (0,7 g/kg), P3 (0,9 g/kg pelet). Ikan dipelihara selama 45 hari dalam akuarium berukuran 40x30x30 cm dengan padat tebar 1 ekor/ 3 L air. Uji tantang dengan A. hydrophila kepadatan 108 CFU/mL sebanyak 0,1 mL/ekor dilakukan pada hari ke-30. Setelah uji tantang ikan kembali dipelihara hingga hari ke- 45. Pada perlakuan Kp dan P1 menunjukkan gejala klinis berupa produksi lendir berlebih, warna pucat, pergerakan tidak normal, mata buram, nafsu makan berkurang, dan sirip geripis. Pemberian pakan mengandung kunyit pada ikan lele dumbo untuk mencegah infeksi A. hydrophila. Dosis terbaik pemberian pakan mengandung kunyit adalah 0,9 g/kg pelet yang ditandai dengan total leukosit 11,29x104 sel/mm3, kadar leukokrit 1,33%, limfosit 79,66%, monosit 12,00%, neutrofil 7,00%, aktivitas fagositosis 34,66%, dan kelulushidupan ikan 80,00%.

Aeromonas hydrophila-induced alterations in cytosolic calcium activate pro-apoptotic cPKC-MEK1/2-TNFα axis in infected headkidney macrophages of Clarias gariepinus

2017 ◽  
Vol 76 ◽  
pp. 392-402 ◽  
Author(s):  
Asha Shelly ◽  
Chaitali Banerjee ◽  
Gunjan Kumar Saurav ◽  
Atish Ray ◽  
Vipin Singh Rana ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Clarias Gariepinus ◽  
Cytosolic Calcium

scholarly journals Effect of putative probiont Enterococcus hirae on the hematological parameters of juvenile African catfish, Clarias gariepinus (Burchell, 1822) during pre- and post-challenge against Aeromonas hydrophila

2018 ◽  
Vol 14 (3) ◽  
pp. 423-428 ◽  
Author(s):  
Nur Hidayahanum Hamid ◽  
Hassan Mohd Daud ◽  
Prapansak Srisapoome ◽  
Hasliza Abu Hassim ◽  
Md Sabri Mohd Yusoff ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Mung Bean ◽  
Hematological Parameters ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Post Challenge ◽  
Control Groups ◽  
Blood Samples ◽  
Mung Bean Sprouts ◽  
Bean Sprouts

Probiotics have been widely known to have the ability to improve the immune system of livestock and aquatic animal. The present study was carried out to evaluate the effect of dietary supplementation of two probiotic isolates of Enterococcus faecium on hematological parameters of juvenile African catfish, Clarias gariepinus during pre- and post-challenge with aquatic pathogen, Aeromonas hydrophila. The probiotics were previously isolated from vegetable wastes (mung bean sprouts, Vigna radiate and cucumber, Cucumis sativus) which have been fermented for 7 days. The experimental fish (270 tails) with an average weight of 5.13 ± 1.03 g were distributed and divided randomly  into  i) control (30 tails), fed with commercial diet ii) E1 (30 tails), fed with  diets  supplemented  with  108 CFU/ml  of  E. faecium isolated from fermented cucumber, iii) E2 (30 tails), fed with 108 CFU/ml  of  E. faecium isolated from fermented mung bean sprouts. The feeding trial was conducted for 50 days. All experimental groups were then challenged with A. hydrophila (1.5 × 106 CFU/mL) via intraperitoneal injection on day 51. Prior to challenge, blood samples were collected from five fish randomly selected from each group on the day 51 (pre-challenge).  After 72 hours of post-challenge, blood samples were again collected from five fish from each groups. The hematological parameters such as total erythrocyte count  (RBC),  total  leucocyte  count  (WBC),  packed cell volume (PCV),  hemoglobin  (Hb), the derived blood indices of mean corpuscular volume (MCV) and corpuscular hemoglobin concentration (MCHC) were examined.  Hematological profiles of pre- and post-challenge infected juvenile catfish were compared with the control groups. The RBC, Hb, WBC, PCV, MCV and MCHC of fish fed with probiotics showed higher significant difference (P<0.05) as compared to control groups during pre- and post-challenge of pathogen. The high level of RBC and WBC during pre- and post-challenge showed the capability of the probiotics to improve the immune response of juvenile African catfish and thus increased the fish disease resistance against A. hydrophila infection. The result suggested that E. faecium could be used effectively as a probiotic in aquaculture.

scholarly journals Effectiveness ambon banana stem juice as immunostimulatory against Aeromonas hydrophila infections in catfish Clarias gariepinus

2017 ◽  
Vol 16 (2) ◽  
pp. 165
Author(s):  
Qorie Astria ◽  
Sri Nuryati ◽  
Kukuh Nirmala ◽  
Alimuddin Alimuddin
Keyword(s):  
Immune System ◽  
Aeromonas Hydrophila ◽  
Bacterial Infections ◽  
Clarias Gariepinus ◽  
Challenge Test ◽  
Positive Control ◽  
Feed Conversion ◽  
High Death ◽  
Banana Stem ◽  
L 13

<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">Outbreaks of infectious diseases due to <em>Aeromonas hydrophila</em> in catfish can cause high death rates (80–100%). Fish disease control can be done using phytopharmaceutical to prevent or treat diseases of fish. One of the phytopharmaceutical that known to prevent the fish diseases is ambon banana stem <em>Musa cavendishii</em> var. dwarf Paxton. This study was conducted to test the effectiveness of catfish immersion using banana stem juice as an immunostimulant against bacterial infections <em>A. hydrophila</em>. The observed parameters were a total performance of production, hematological test, and water quality. Immersion of catfish seeds with stem juice was performed at a concentration of 5 mL/L, 13 mL/L, and 21 mL/L for 30 minutes. Each treatment consisted of three replications. A  total of 15 fishes were immersed in 1.5 L water. At day-9 after immersion, fish was infected by <em>A. hydrophila</em> bacteria at a dose of 10<sup>4</sup> cfu/mL. The results showed that fish treated with banana stem juice at a concentration of 13 mL/L had the survival rate of 53.33±6.67% which is higher than positive control (33.33±6.67%). Based on hematology observations on the 3rd day post-challenge test (H12) with <em>A. hydrophila</em>, total erythrocytes, hemoglobin, total leukocytes, phagocytic activity, differential leukocyte, and lysozyme activity was highest in treatment13 mL/L. Lower feed conversion ratio (1.08±0.04) were also obtained in treatment 13 mL/L. Thus Immersion of seeds in stem juice can boost the immune system against infections <em>A. hydrophila</em>.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: <em>Aeromonas hydrophila</em>, ambon banana stem juice, soaking, catfish, immune system</p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong>ABSTRAK</strong><strong></strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">Wabah penyakit akibat infeksi <em>Aeromonas hydrophila</em> pada ikan lele dapat menyebabkan tingkat kematian yang tinggi (80–100%). Penanggulangan penyakit ikan dapat dilakukan menggunakan fitofarmaka untuk mencegah ataupun mengobati penyakit ikan. Salah satu fitofarmaka yang dapat digunakan dalam upaya pencegahan penyakit ikan adalah batang pisang ambon lumut <em>Musa cavendishii</em> var. dwarf Paxton. Penelitian ini bertujuan untuk menguji efektivitas perendaman benih ikan lele dengan menggunakan air perasan batang pisang ambon sebagai imunostimulan terhadap infeksi bakteri <em>A. hydrophila</em>. Parameter yang diamati selama penelitian adalah kinerja produksi, uji hematologi dan pengukuran kualitas air. Perendaman benih ikan lele dengan air perasan  dilakukan pada konsentrasi 5 mL/L, 13 mL/L, dan 21 mL/L selama 30 menit. Pada hari ke-9 setelah perendaman, ikan diinfeksi bakteri <em>A. hydrophila </em>pada kepadatan 10<sup>4</sup> cfu/mL. Hasil penelitian menunjukkan bahwa perendaman ikan dengan air perasan pada konsentrasi 13 mL/L memiliki kelangsungan hidup yakni sebesar 53,33±6,67%, lebih tinggi bila dibandingkan dengan kontrol positif  (33,33±6,67%). Berdasarkan pengamatan pada uji hematologi hari ke-3 pascauji tantang (H12) dengan bakteri <em>A. hydrophila</em>, total eritrosit, hemoglobin, total leukosit, aktivitas fagositik, dan aktivitas lisozim tertinggi terdapat pada perlakuan konsentrasi 13 mL/L. Rasio konversi pakan yang rendah (1,08±0,04) juga terdapat pada perlakuan 13 mL/L. Hal ini menunjukkan bahwa perendaman benih ikan lele pada air perasan dapat meningkatkan sistem imun terhadap infeksi bakteri <em>A. hydrophila</em>.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: <em>Aeromonas hydrophila</em>, air perasan batang pisang ambon, perendaman, ikan lele, sistem imun</p>

scholarly journals Effectiveness of ambon banana stem juice as immunostimulatory against Aeromonas hydrophila infections in catfish Clarias gariepinus

2017 ◽  
Vol 16 (2) ◽  
pp. 154 ◽  
Author(s):  
Qorie Astria ◽  
Sri Nuryati ◽  
Kukuh Nirmala ◽  
Alimuddin Alimuddin
Keyword(s):  
Immune System ◽  
Aeromonas Hydrophila ◽  
Bacterial Infections ◽  
Clarias Gariepinus ◽  
Challenge Test ◽  
Positive Control ◽  
Feed Conversion ◽  
High Death ◽  
Banana Stem ◽  
L 13

<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">Outbreaks of infectious diseases due to <em>Aeromonas hydrophila</em> in catfish can cause high death rates (80–100%). Fish disease control can be done using phytopharmaceutical to prevent or treat diseases of fish. One of the phytopharmaceutical that known to prevent the fish diseases is ambon banana stem <em>Musa cavendishii</em> var. dwarf Paxton. This study was conducted to test the effectiveness of catfish immersion using banana stem juice as an immunostimulant against bacterial infections <em>A. hydrophila</em>. The observed parameters were a total performance of production, hematological test, and water quality. Immersion of catfish seeds with stem juice was performed at a concentration of 5 mL/L, 13 mL/L, and 21 mL/L for 30 minutes. Each treatment consisted of three replications. A  total of 15 fishes were immersed in 1.5 L water. At day-9 after immersion, fish was infected by <em>A. hydrophila</em> bacteria at a dose of 10<sup>4</sup> cfu/mL. The results showed that fish treated with banana stem juice at a concentration of 13 mL/L had the survival rate of 53.33±6.67% which is higher than positive control (33.33±6.67%). Based on hematology observations on the 3rd day post-challenge test (H12) with <em>A. hydrophila</em>, total erythrocytes, hemoglobin, total leukocytes, phagocytic activity, differential leukocyte, and lysozyme activity was highest in treatment13 mL/L. Lower feed conversion ratio (1.08±0.04) were also obtained in treatment 13 mL/L. Thus Immersion of seeds in stem juice can boost the immune system against infections <em>A. hydrophila</em>.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: <em>Aeromonas hydrophila</em>, ambon banana stem juice, soaking, catfish, immune system</p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong>ABSTRAK</strong><strong></strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">Wabah penyakit akibat infeksi <em>Aeromonas hydrophila</em> pada ikan lele dapat menyebabkan tingkat kematian yang tinggi (80–100%). Penanggulangan penyakit ikan dapat dilakukan menggunakan fitofarmaka untuk mencegah ataupun mengobati penyakit ikan. Salah satu fitofarmaka yang dapat digunakan dalam upaya pencegahan penyakit ikan adalah batang pisang ambon lumut <em>Musa cavendishii</em> var. dwarf Paxton. Penelitian ini bertujuan untuk menguji efektivitas perendaman benih ikan lele dengan menggunakan air perasan batang pisang ambon sebagai imunostimulan terhadap infeksi bakteri <em>A. hydrophila</em>. Parameter yang diamati selama penelitian adalah kinerja produksi, uji hematologi dan pengukuran kualitas air. Perendaman benih ikan lele dengan air perasan  dilakukan pada konsentrasi 5 mL/L, 13 mL/L, dan 21 mL/L selama 30 menit. Pada hari ke-9 setelah perendaman, ikan diinfeksi bakteri <em>A. hydrophila </em>pada kepadatan 10<sup>4</sup> cfu/mL. Hasil penelitian menunjukkan bahwa perendaman ikan dengan air perasan pada konsentrasi 13 mL/L memiliki kelangsungan hidup yakni sebesar 53,33±6,67%, lebih tinggi bila dibandingkan dengan kontrol positif  (33,33±6,67%). Berdasarkan pengamatan pada uji hematologi hari ke-3 pascauji tantang (H12) dengan bakteri <em>A. hydrophila</em>, total eritrosit, hemoglobin, total leukosit, aktivitas fagositik, dan aktivitas lisozim tertinggi terdapat pada perlakuan konsentrasi 13 mL/L. Rasio konversi pakan yang rendah (1,08±0,04) juga terdapat pada perlakuan 13 mL/L. Hal ini menunjukkan bahwa perendaman benih ikan lele pada air perasan dapat meningkatkan sistem imun terhadap infeksi bakteri <em>A. hydrophila</em>.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: <em>Aeromonas hydrophila</em>, air perasan batang pisang ambon, perendaman, ikan lele, sistem imun</p>

scholarly journals Pengaruh pemberian mikrokapsul probiotik Bacillus cereus P22 dan Staphylococcus lentus L1k pada pakan terhadap kinerja pertumbuhan, respons imun, dan resistensi ikan lele, Clarias gariepinus Burchell 1822 yang diinfeksi Aeromonas hydrophila

2018 ◽  
Vol 17 (2) ◽  
pp. 143
Author(s):  
Lilik Setiyaningsih ◽  
Widanarni Widanarni ◽  
Angela Mariana Lusiastuti3 ◽  
Munti Yuhana
Keyword(s):  
Bacillus Cereus ◽  
Growth Performance ◽  
Aeromonas Hydrophila ◽  
Clarias Gariepinus ◽  
Bacterial Count ◽  
Negative Control ◽  
Total Bacterial Count ◽  
African Catfish ◽  
Feed Conversion ◽  
Significant Difference

The aimed of this research was to evaluate the effects of dietary probiotic microcapsules B.cereus P22 and S. lentus (L1k) at different dose and frequency on growth performance, immune response and resistance of African catfish infected with A. Hydrophila. Probiotics used in this study were B. cereus P22 and S. lentus L1k encapsulated by spray draying method. The research was carried out for 56 days with eight treatments and four replications. The treatments were K- (negative control), K+ (positive control), A (feed supplemented with 0,5% of microencapsulated probiotic, fedevery day), B (feed supplemented with 0,5% of microencapsulated probiotic, fed once every three days), C (feed supplemented with 1% of microencapsulated probiotic, fed every day), D (feed supplemented with 1% of microencap-sulated probiotic with an administration once every three days), E (feed with 2% of microencapsulated probiotic with an administration every day) and F (feed with 2% of microencapsulated probiotic with an administration once every three days). On day 42, all of the fish except K- were challenged by intramuscular injection of A. hydrophila (108 CFU ml-1). In 40 days after infection, there were no significant difference on survival rate (SR) between treatments (p> 0.05). Treatment E displayed the higher growth rate (4,54±0,02%), total probiotic B. cereus (P22) and S . lentus (L1k) (4.06± 0.09 log CFU g-1; 4.02±0.08 log CFU g-1) than other treatments; whereas treatment D showed the best feed conversion rasio(1.191±0.013), and treatment F offered the highest total bacterial count (7.11±0.53 log CFU g-1). An administration of 2% microencapsulated probiotic in every day frequency demonstrated the better growth performance, immune res-ponse and resistance of African catfish to A. hydrophila compare with other treatments. AbstrakPenelitian ini bertujuan untuk mengkaji dosis dan frekuensi pemberian mikrokapsul probiotik melalui pakan terhadap kinerja pertumbuhan, respons imun, dan resistensi ikan lele yang diinfeksi Aeromonas hydrophila. Penelitian ini meng-gunakan Bacillus cereus P22 dan Staphylococcus lentus L1k yang telah dienkapsulasi melalui metode spray draying. Penelitian ini dilaksankan selama 56 hari dengan delapan perlakuan dan empat ulangan, terdiri atas perlakuan K- (kon-trol negatif), K+ (kontrol positif), A (pakan+mikrokapsul probiotik dosis 0,5% frekuensi setiap hari), B (pakan+mikro-kapsul probiotik dosis 0,5% frekuensi tiga hari sekali), C (pakan+mikrokapsul probiotik dosis 1% frekuensi setiap hari), D (pakan+mikrokapsul probiotik dosis 1% frekuensi tiga hari sekali), E (pakan+mikrokapsul probiotik dosis 2% fre-kuensi setiap hari) dan F (pakan+mikrokapsul probiotik dosis 2% frekuensi tiga hari sekali). Ikan diuji tantang dengan A. hydrophila pada hari ke 42 dengan kepadatan 108 CFU ml-1 secara intramuscular (kecuali K- diinjeksi dengan phos-phate buffer saline). Setelah 40 hari pascainjeksi, laju sintasan tidak menunjukkan perbedaan yang signifikan antarperlakuan (p>0,05).Perlakuan E menunjukkan laju pertumbuhan (4,54±0,02%) dan total probiotik B. cereus (P22) dan S. lentus (L1k) (4,06±0,09 log CFU g-1; 4,02±0,08 log CFU g-1) tertinggi; sementara perlakuan D memberikan hasil nisbah konversi pakan terbaik (1,191±0,013), perlakuan F menunjukkan total bacterial count tertinggi (7,11±0,53 log CFU g-1). Hasil penelitian menunjukkan bahwa dosis 2% yang diberikan setiap hari memberikan hasil yang lebih baik dalam meningkatkan laju pertumbuhan, respons imun, dan resistensi ikan lele terhadap A. hydrophila. 

scholarly journals White Mulberry (Morus alba) Foliage Methanolic Extract Can AlleviateAeromonas hydrophilaInfection in African Catfish (Clarias gariepinus)

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Atefeh Sheikhlar ◽  
Abd Razk Alimon ◽  
Hassan Daud ◽  
Chee R. Saad ◽  
Carl D. Webster ◽  
...  
Keyword(s):  
Total Protein ◽  
Aeromonas Hydrophila ◽  
Dry Matter ◽  
Clarias Gariepinus ◽  
Infected Fish ◽  
Morus Alba ◽  
African Catfish ◽  
Growth Promoter ◽  
White Mulberry ◽  
And Control

Two experiments were simultaneously conducted withMorus alba(white mulberry) foliage extract (MFE) as a growth promoter and treatment ofAeromonas hydrophilainfection in separate 60 and 30 days trail (Experiments 1 and 2, resp.) in African catfish (Clarias gariepinus). In Experiment 1, four diets, control and control supplemented with 2, 5, or 7 g MFE/kg dry matter (DM) of diet, were used. In Experiment 2, fish were intraperitoneally infected withAeromonas hydrophilaand fed the same diets as experiment 1 plus additional two diets with or without antibiotic. Results of experiment 1 showed that growth was unaffected by dietary levels of MFE. Treatments with the inclusion of MFE at the levels of 5 and 7 g/Kg DM had no mortality. Red blood cells (RBC), albumin, and total protein were all higher for the treatments fed MFE (5 and 7 g/Kg DM). Results of experiment 2 showed RBC, hemoglobin, hematocrit, globulin, albumin, and total protein improved with the increase in MFE in the infected fish. The dietary MFE at the level of 7 g/kg DM reduced mortality rate. In conclusion, MFE at the level of 7 g/kg DM could be a valuable dietary supplement to cure the infected fish.

Sign in / Sign up

Export Citation Format

Share Document