scholarly journals Optimization of preservation solutions to execute testing on cervical smear sample

2021 ◽  
Vol 12 (6) ◽  
pp. 34-37
Author(s):  
Quang Thanh Le ◽  
Thanh Hai Pham ◽  
Quy Thi Cam Nguyen ◽  
Quoc Phong Truong ◽  
Thi Thanh Tam Nguyen ◽  
...  
Keyword(s):  
Laboratory Testing ◽  
Room Temperature ◽  
Freeze Solution ◽  
Three Solutions ◽  
Cervical Sample ◽  
Preservation Solutions ◽  
Cold Condition ◽  
The Common ◽  
Cervical Cells ◽  
Protein Measurement

Background: The common methods to preserve cell for protein analyses are in cold condition or treated with freeze solution and packaging in dry ice for shipping. Solution which can preserve cervical cells at room temperature is preferable and cost consuming for laboratory testing. Aims and Objective: Research and optimized the storage and transport solution for cervical sample which can preserve cells at room temperature for laboratory testing. Materials and Methods: In this study, cervical specimens were collected in 3 different preservation solutions. Storage and transport of samples was at ambient or refrigerated temperature. The effect of preservation solution and temperature was check by cell visualization under microscope and protein measurement. Results: Presence of cells were detected in all three solutions. Among those, HEPES solution can preserve the highest number of cells and at room temperature. Conclusion: HEPES solution appeared suitable to preserve cervical cytology specimens at ambient temperature for further laboratory testing at protein and DNA level.

Molecule-Based Magnets—An Overview

MRS Bulletin ◽  
2000 ◽  
Vol 25 (11) ◽  
pp. 21-30 ◽  
Author(s):  
Joel S. Miller ◽  
Arthur J. Epstein
Keyword(s):  
Low Temperature ◽  
Vapor Deposition ◽  
Room Temperature ◽  
Chemical Vapor ◽  
Magnetic Ordering ◽  
Low Density ◽  
Materials Properties ◽  
The Common ◽  
New Processing ◽  
Very High

Molecule-based magnets are a broad, emerging class of magnetic materials that expand the materials properties typically associated with magnets to include low density, transparency, electrical insulation, and low-temperature fabrication, as well as combine magnetic ordering with other properties such as photoresponsiveness. Essentially all of the common magnetic phenomena associated with conventional transition-metal and rare-earth-based magnets can be found in molecule-based magnets. Although discovered less than two decades ago, magnets with ordering temperatures exceeding room temperature, very high (∼27.0 kOe or 2.16 MA/m) and very low (several Oe or less) coercivities, and substantial remanent and saturation magnetizations have been achieved. In addition, exotic phenomena including photoresponsiveness have been reported. The advent of molecule-based magnets offers new processing opportunities. For example, thin-film magnets can be prepared by means of low-temperature chemical vapor deposition and electrodeposition methods.

Growth and Heat Resistance of Salmonella enteritidisin Refrigerated and Abused Eggs

1993 ◽  
Vol 56 (11) ◽  
pp. 927-931 ◽  
Author(s):  
A. MAHDI SAEED ◽  
CAROL W. KOONS
Keyword(s):  
Heat Resistance ◽  
Salmonella Enteritidis ◽  
Room Temperature ◽  
Storage Temperature ◽  
Phase 1 ◽  
Shell Eggs ◽  
Growth Inhibitory ◽  
Significant Barrier ◽  
The Common ◽  
Salmonella Survival

The effect of storage temperature on the growth and heat resistance of Salmonella enteritidis (SE) in eggs artificially inoculated with 20 CFU per egg has been investigated. SE organisms grew to a stationary phase (1 × 109 CFU/ml of egg) within 2–3 d after storing the eggs at room temperature (23°C), while minimal or no growth occurred in similarly inoculated eggs that were refrigerated at 4°C. None of the common methods of egg cooking was found totally effective in eliminating SE organisms in massively contaminated eggs. SE organisms survived in significantly higher proportions of the eggs that were stored at 23°C than in refrigerated eggs after cooking by different methods (P < 0.05). Higher rates of Salmonella survival after cooking were detected in the eggs that were stored at room temperature for 5 d or longer than in eggs stored for shorter time (P < 0.05). Data from this study indicated: i) storage abuse of eggs can be a major factor in increasing the prevalence of eggborne SE infections by increasing the infectious load and heat resistance of the SE organisms in the contaminated eggs, and ii) that breaking and pooling of eggs immediately before cooking can minimize the substantial multiplication of SE organisms from slightly contaminated eggs. Refrigeration, therefore, can be a significant barrier against SE infections through its growth inhibitory and heat resistance-reducing effects on the SE organisms in contaminated shell eggs as well as in large volumes of liquid eggs intended for pasteurization.

Surface oxygen vacancies on Co3O4 mediated catalytic formaldehyde oxidation at room temperature

2016 ◽  
Vol 6 (11) ◽  
pp. 3845-3853 ◽  
Author(s):  
Zhong Wang ◽  
Wenzhong Wang ◽  
Ling Zhang ◽  
Dong Jiang
Keyword(s):  
Catalytic Oxidation ◽  
Room Temperature ◽  
Oxygen Vacancies ◽  
Essential Role ◽  
Surface Oxygen ◽  
Oxidation Reactions ◽  
Major Activity ◽  
Formaldehyde Oxidation ◽  
The Common

This study reveals the essential role played by surface oxygen vacancies in catalytic oxidation reactions, and complements the common viewpoint that Co3+ is the major activity species in Co3O4-based systems.

Electroluminescence from GalnN Quantum Wells Grown on Non-(0001) Facets of Selectively Grown GaN Stripes

2004 ◽  
Vol 831 ◽  
Author(s):  
Barbara Neubert ◽  
Frank Habel ◽  
Peter Bruckner ◽  
Ferdinand Scholz ◽  
Till Riemann ◽  
...  
Keyword(s):  
Low Temperature ◽  
Electric Field ◽  
Quantum Wells ◽  
Room Temperature ◽  
Light Emission ◽  
Low Pressure ◽  
Lower Efficiency ◽  
Strong Light ◽  
The Common ◽  
Piezo Electric

ABSTRACTNon (0001) GalnN QWs have been grown by low pressure MOVPE on side facets of triangular shaped selectively grown GaN stripes. By analysing low temperature photo- and cathodoluminescence and room temperature electroluminescence, we found strong indications, that both, In and Mg are less efficiently incorporated on these side facets compared to the common (0001) plane with even lower efficiency for stripes running along (1–100) compared to (11–20). Nevertheless, we observed strong light emission from these quantum wells, supposed to be at least partly caused by the reduced piezo-electric field.

scholarly journals Sensitivity of shotcrete Brazilian discs coated with thin spray-on liners to environmental conditions

2021 ◽  
Vol 121 (7) ◽  
Author(s):  
M.J. Kanda ◽  
T.R. Stacey
Keyword(s):  
High Temperature ◽  
Environmental Conditions ◽  
Laboratory Testing ◽  
Laboratory Tests ◽  
Room Temperature ◽  
Mining Industry ◽  
High Humidity ◽  
Rock Support ◽  
Strength Enhancement ◽  
Indirect Tensile

SYNOPSIS Thin spray-on liners (TSLs) have been used as sealants and rock support in tunnels for over 25 years. Laboratory tests have indicated satisfactory properties that can provide adequate strength, adhesion, toughness, and elasticity as part of rock support systems in mining excavations. These characteristics are, however, not always demonstrated in practice, when mine environmental conditions do not correspond with laboratory environmental conditions. The shortcomings of TSLs experienced in the mining industry have raised concerns, resulting in scepticism from some operators regarding their utilization. The research described in this paper aims to investigate TSL performance in environmental conditions similar to those experienced in mines. Brazilian indirect tensile (BIT) specimens were prepared from precast shotcrete and coated with TSLs. The specimens were then exposed to different environmental conditions for up to 112 days before BIT testing under various conditions: laboratory room temperature and humidity; saturated room temperature; and combined saturated and high temperature (50°C). Additional uncoated shotcrete and TSL BIT specimens were prepared for comparison purposes. The results of the BIT tests showed that environmental conditions have a significant influence on the tensile strength enhancement of shotcrete by TSLs. Water-based TSLs are most likely to be suitable for high humidity environments, although their performance decreases at higher temperatures. Numerical modelling of TSL-coated BIT samples confirms the potential limitations of designing TSL support based only on laboratory testing carried out under room conditions. Keywords: thin spray-on liner, Brazilian indirect tensile (BIT) test, TSL performance, environmental conditions, humidity, high temperature.

Natural Attenuation as a Decontamination Approach for SARS-CoV-2 on Library Materials

2021 ◽  
Vol 70 (1) ◽  
pp. 65-74
Author(s):  
Maria V. Fedotova
Keyword(s):  
Relative Humidity ◽  
Natural Attenuation ◽  
Room Temperature ◽  
Building Materials ◽  
Library Services ◽  
Coated Steel ◽  
Active Virus ◽  
Glass Marble ◽  
Computer Library ◽  
The Common

Based on the current scientific understanding regarding COVID-19, this article presents the results of new studies (Tests 4, 5, 6), conducted by the Institute of Museum and Library Services (IMLS) and the Online Computer Library Center (OCLC), in partnership with Battelle. The purpose of the experiments is to determine how long frequently circulated library, museum and archival materials should be quarantined before being put back into public circulation.Each test has been conducted by applying the virulent SARS-CoV-2 virus on various materials held at standard room temperature and relative humidity conditions, and then examining the rate of natural attenuation of the virus. The items were not sterilized before testing. Battelle propagated the clinical isolate of the SARS-CoV-2 virus in-house, followed by characterization and testing to establish a certified titer. The materials in Test 4 included binding and cover items placed in a stacked configuration to imitate the common practice that libraries employ when handling book/DVD returns, as well as expanded polyethylene foam, which is typically used in museums for storage and shipping. Test 5 focused on textiles, while Test 6 included building materials (glass, marble, brass, laminate, and powder-coated steel). Test coupons cut from the materials were inoculated with active virus, and then allowed to dry. The test coupons were then examined at the following timepoints: on the second, third, fourth and sixth day (Test 4); on the second, fourth, sixth and eighth day (Tests 5 and 6). The results of the tests showed how much virus was detectable at the selected timepoints. In Test 4, the virus was still detected on all the five materials after six days. In Test 5, the virus was still detected on leather and synthetic leather publication after eight days. In Test 6, the virus was not detected on the tested materials after six days.This publication is provided for informational purposes only, and readers are encouraged to review federal, regional, and local guidance. The authors, sponsors and researchers are not liable for any damages resulting from use, misuse, or reliance upon this information, or any errors or omissions herein.

scholarly journals Interaction of N-alkyl-N-nitrosourethanes with thiols

1965 ◽  
Vol 97 (2) ◽  
pp. 466-474 ◽  
Author(s):  
R Schoental ◽  
DJ Rive
Keyword(s):  
Room Temperature ◽  
Methyl Esters ◽  
Spatial Proximity ◽  
Thiol Compounds ◽  
Primary Products ◽  
Methyl Derivatives ◽  
The Common ◽  
Carcinogenic Process

1. The interaction at room temperature of thiols (cysteine and certain of its derivatives, and glutathione) with N-alkyl-N-nitrosourethanes and with diazomethane gave complicated mixtures of products. 2. S-Methylcysteine and S-ethoxycarbonylcysteine, the main products of the reaction between cysteine and N-methyl-N-nitrosourethane, were isolated and unequivocally identified. Paper-chromatographic and other evidence was used for the identification of several other components of the mixtures of products obtained. 3. S-Methyl derivatives and their methyl esters were the common products formed from the thiol compounds with both N-methyl-N-nitrosourethane and with diazomethane. 4. The esters were unstable and hydrolysed readily. 5. S-Ethoxycarbonyl derivatives, the primary products of the reaction of the thiols with N-alkyl-N-nitrosourethanes, isomerized to the respective N-ethoxycarbonyl derivatives when the pH increased above 7.0; the migration of the ethoxycarbonyl group from S to N was particularly easy with cysteine, probably owing to the spatial proximity of the amino group. 6. It is suggested that the non-enzymic reactions in vitro of thiols with both N-methyl-N-nitrosourethane and diazomethane may represent models for events in vivo and might help in the studies of the carcinogenic process.

Fabrication and Optical Properties of Mn Doped ZnS Nanowires

2011 ◽  
Vol 236-238 ◽  
pp. 2211-2215
Author(s):  
Jun Jie Qi ◽  
Qi Zhang ◽  
Qing Liang Liao ◽  
Zhan Qiang Deng ◽  
Yue Zhang
Keyword(s):  
Optical Properties ◽  
Aspect Ratio ◽  
Room Temperature ◽  
Green Emission ◽  
Deposition Method ◽  
Photoluminescence Properties ◽  
Room Temperature Photoluminescence ◽  
The Common ◽  
Strong Green Emission ◽  
Mn Doped

Mn doped ZnS nanowires with typical wurtzite single-crystalline structure were successfully synthesized via H2 assistant chemical evaporation deposition method. The investigations indicated that the diameter of Mn/ZnS Nanowire with high aspect ratio is 25~40nm, EDS results show that the content of Mn element is about 4.45at%. HRTEM and SAED results demonstrated that the Mn/ZnS nanowires grow along [101] direction, which was different from the common direction reported in literatures. Room temperature photoluminescence properties were also examined, showing a strong green emission centered at 523.04 nm, and a weak emission at 382.53 nm was also observed, showing a red shift of 45.53 nm comparing to the intrinsic luminescence.

scholarly journals BACTERIUM GRANULOSIS IN RELATION TO TRACHOMA: ITS RECOVERY FROM EXPERIMENTALLY INFECTED MONKEYS AND FROM HUMAN TRACHOMA

1930 ◽  
Vol 52 (4) ◽  
pp. 617-636 ◽  
Author(s):  
E. B. Tilden ◽  
J. R. Tyler
Keyword(s):  
Cultural Study ◽  
Room Temperature ◽  
Bactericidal Effect ◽  
Biological Characteristics ◽  
Rabbit Serum ◽  
Semisolid Medium ◽  
Negative Results ◽  
Indian Schools ◽  
The Common

One of the strains of Bacterium granulosis isolated by Noguchi in 1926 has been maintained in culture and in monkeys and continues to be capable, after 3 years, of inducing a chronic granular conjunctivitis in monkeys. Cultures of this strain have been recovered from the monkey lesions as late as 3 years after inoculation and have been shown to reproduce the granular disease in monkeys. Six additional strains of Bacterium granulosis have been isolated from cases of trachoma occurring in the Indian schools of Arizona. The cultures thus obtained are identical morphologically and culturally with those isolated by Noguchi and have induced the same chronic granular conjunctivitis in monkeys. Advanced untreated cases are more favorable for cultural study than treated cases. Cultures of Bacterium granulosis kept on semisolid medium containing 10 per cent rabbit serum ("leptospira medium") remain viable for many months at room temperature, and sealed ampoules of such cultures have been found to retain their pathogenicity for the monkey conjunctiva for at least 69 days at room temperature and at least 284 days at 4° to 6°C. Several additional cultural and biological characteristics of Bacterium granulosis have been described. Of outstanding importance is the fact that cocaine, in contradistinction to novocain, has a bactericidal effect on the organism. This fact, in view of the common use of cocaine for anesthesia, may explain the negative results of cultivation experiments reported by some workers.

Rate Controlling Steps of SCC of High Strength Steels

CORROSION ◽  
1979 ◽  
Vol 35 (9) ◽  
pp. 397-402 ◽  
Author(s):  
B. STELLWAG ◽  
H. KAESCHE
Keyword(s):  
Stress Intensity ◽  
Crack Growth ◽  
Room Temperature ◽  
High Strength Steels ◽  
High Strength ◽  
Intensity Curve ◽  
Plateau Region ◽  
The Common ◽  
Cracking Process ◽  
Emission Studies

Abstract Stress corrosion crack velocities have been measured for a high strength steel in 0.5N NaCI solution at room temperature without potentiostatic control. Changing the strength level of the steel alters the shape of the SCC velocity vs stress intensity curve distinctly. With increasing strength, the common plateau region (so called stage II) disappears, and SCC velocity becomes exponentially dependent on stress intensity. Additional experiments (acoustic emission studies, crack growth incubation measurements, load change tests, fractography) confirm the current understanding, that absorbed hydrogen is the damaging species. The results can be well interpreted by assuming that lattice decohesion of the metallic bonds by absorbed hydrogen is a fundamental feature of the cracking process. Conclusions can be drawn as to the rate controlling steps of crack growth.

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