Characterization of Diazotrophic Rhizobacteria under Various Conditions

Nitrogenase activity was analysed after supplementation of various carbon and nitrogen sources in the growth medium by Acetylene Reduction Assay methods in selected 9 isolates from 74 diazotrophic isolates. Enhancement in nitrogenase activity was recorded many fold by the addition of different organic carbon sources in which maltose and pyruvate showed better result than others. In case of supplementation of nitrogen sources, reduction of nitrogenase activity was observed. Nitrogenase activity increased from 22.7 to 72.7% in various strains when they are tested in anaerobic condition, Amplification of fragment of 390 bp showed that nitrogenase activity due to presence of nifH gene. Sequences were submitted to NCBI GeneBank and the accession number of nifH sequence of ECI-10A (FJ032023), AF-4B (FJ032024), AF-4C (FJ032022) and BN-2A (FJ032021) has been obtained. Phylogenetic analysis based on showed that these 4 isolates belong to the member of γ-proteobacteria, but show appreciable genetic diversity.DOI: http://dx.doi.org/10.3126/ijasbt.v1i3.8607 Int J Appl Sci Biotechnol, Vol. 1(3) 2013 : 110-117

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Isolation, Screening, Characterization of Indigenous Oleaginous Bacteria: Evaluation of Various Carbon and Nitrogen Sources as Substrates for Single Celled Oil Producing Bacteria

Asian Journal of Biotechnology and Bioresource Technology ◽

10.9734/ajb2t/2018/39260 ◽

2018 ◽

Vol 3 (1) ◽

pp. 1-12 ◽

Cited By ~ 1

Author(s):

Kulvinder Bajwa ◽

Narsi Bishnoi ◽

Manju Toor ◽

Saloni Gupta ◽

Pankaj Sharma ◽

...

Keyword(s):

Nitrogen Sources ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen

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Effects of carbon and nitrogen sources on production of proteases by Bacillus subtilis IC-5

Bangladesh Journal of Botany ◽

10.3329/bjb.v44i2.38518 ◽

2018 ◽

Vol 44 (2) ◽

pp. 285-292

Author(s):

Sereen Gul ◽

Mujeeb Ur Rahman ◽

Mohammad Ajmal ◽

Abdul Kabir Khan Achakzai ◽

Asim Iqbal

Keyword(s):

Bacillus Subtilis ◽

Sodium Nitrate ◽

Inorganic Nitrogen ◽

Carbon Sources ◽

Basal Medium ◽

Nitrogen Sources ◽

Neutral Protease ◽

Carbon And Nitrogen Sources ◽

Inorganic Nitrogen Source ◽

Carbon And Nitrogen

The effects of various carbon and nitrogen sources were evaluated on production of proteases by Bacillus subtilis IC-5. Both type and concentration of carbon and nitrogen sources influenced the production of proteases. Among the carbon sources glucose was found to be the most effective. It gave maximum production at 2% w/v concentration i.e., 1875 and 950 U/ml, alkaline and neutral protease, respectively. The response of Bacillus subtilis IC-5 towards synthesis and excretion of enzymes varied with the type of nitrogen sources. The addition of organic nitrogen sources to basal medium repressed the synthesis of proteases while the addition of inorganic nitrogen source such as sodium nitrate was found to be the best stimulating for alkaline and neutral protease synthesis. Sodium nitrate enhanced the production up to 62.40 and 10.52% of alkaline and neutral protease, respectively against w.r.t. control.

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Yarrowia lipolyticaand Its Multiple Applications in the Biotechnological Industry

The Scientific World JOURNAL ◽

10.1155/2014/476207 ◽

2014 ◽

Vol 2014 ◽

pp. 1-14 ◽

Cited By ~ 70

Author(s):

F. A. G. Gonçalves ◽

G. Colen ◽

J. A. Takahashi

Keyword(s):

Carbon Sources ◽

Nitrogen Sources ◽

Carbon And Nitrogen Sources ◽

Biotechnological Potential ◽

Carbon And Nitrogen ◽

Intracellular Lipids ◽

Metabolites Production ◽

Growing Conditions ◽

Fermentation Parameters

Yarrowia lipolyticais a nonpathogenic dimorphic aerobic yeast that stands out due to its ability to grow in hydrophobic environments. This property allowed this yeast to develop an ability to metabolize triglycerides and fatty acids as carbon sources. This feature enables using this species in the bioremediation of environments contaminated with oil spill. In addition,Y. lipolyticahas been calling the interest of researchers due to its huge biotechnological potential, associated with the production of several types of metabolites, such as bio-surfactants,γ-decalactone, citric acid, and intracellular lipids and lipase. The production of a metabolite rather than another is influenced by the growing conditions to whichY. lipolyticais subjected. The choice of carbon and nitrogen sources to be used, as well as their concentrations in the growth medium, and the careful determination of fermentation parameters, pH, temperature, and agitation (oxygenation), are essential for efficient metabolites production. This review discusses the biotechnological potential ofY. lipolyticaand the best growing conditions for production of some metabolites of biotechnological interest.

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Response of Cold-Tolerant Aspergillus spp. to Solubilization of Fe and Al Phosphate in Presence of Different Nutritional Sources

ISRN Soil Science ◽

10.1155/2013/598541 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 7

Author(s):

K. Rinu ◽

Mukesh Kumar Malviya ◽

Priyanka Sati ◽

S. C. Tiwari ◽

Anita Pandey

Keyword(s):

Phosphate Solubilization ◽

Carbon Sources ◽

Nitrogen Sources ◽

Iron Phosphate ◽

Fungal Species ◽

Growth Promoters ◽

Aluminium Phosphate ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Phosphate Solubilizing

Three species of Aspergillus, namely, A. niger, A. glaucus and A. sydowii, isolated from soil samples collected from the Indian Himalayan Region (IHR), have been investigated for solubilization of aluminium phosphate and iron phosphate in the presence of different carbon and nitrogen sources. Preference of each fungal species varied for nitrogen and carbon sources, in terms of phosphate-solubilization. Among three species, Aspergillus niger gave the best results; it solubilized 32% and 8% of the supplemented aluminium phosphate and iron phosphate, respectively. The results indicated that the effect of carbon and nitrogen sources can influence the phosphate solubilizing efficiency of all the three Aspergillus spp. tested. All the three species were found to be plant-growth promoters in bioassays conducted under greenhouse conditions. The Al and Fe phosphate solubilization efficiency, investigated in the present study, is at the lower end of their previously reported tricalcium phosphate solubilization efficiency. The cultures are likely to have better field applications in agrobiotechnology, due to their potential towards solubilization of Al and Fe phosphates, which are known to have lower solubility through microbial activity.

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Microbial Cellulose Production from Bacteria Isolated from Rotten Fruit

International Journal of Polymer Science ◽

10.1155/2015/280784 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 23

Author(s):

B. E. Rangaswamy ◽

K. P. Vanitha ◽

Basavaraj S. Hungund

Keyword(s):

Biochemical Analysis ◽

Nitrogen Sources ◽

Growth Conditions ◽

Inoculum Density ◽

Bacterial Isolates ◽

Carbon And Nitrogen Sources ◽

Cellulose Production ◽

Microbial Cellulose ◽

Carbon And Nitrogen

Microbial cellulose, an exopolysaccharide produced by bacteria, has unique structural and mechanical properties and is highly pure compared to plant cellulose. Present study represents isolation, identification, and screening of cellulose producing bacteria and further process optimization. Isolation of thirty cellulose producers was carried out from natural sources like rotten fruits and rotten vegetables. The bacterial isolates obtained from rotten pomegranate, rotten sweet potato, and rotten potato were identified asGluconacetobactersp. RV28,Enterobactersp. RV11, andPseudomonassp. RV14 through morphological and biochemical analysis. Optimization studies were conducted for process parameters like inoculum density, temperature, pH, agitation, and carbon and nitrogen sources usingGluconacetobactersp. RV28. The strain produced 4.7 g/L of cellulose at optimum growth conditions of temperature (30°C), pH (6.0), sucrose (2%), peptone (0.5%), and inoculum density (5%). Characterization of microbial cellulose was done by scanning electron microscopy (SEM).

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A COMPARISON OF ISOLATES OF CLAVICEPS SPP. FOR THE ABILITY TO GROW AND TO PRODUCE ERGOT ALKALOIDS ON CERTAIN NUTRIENTS

Canadian Journal of Microbiology ◽

10.1139/m60-040 ◽

1960 ◽

Vol 6 (3) ◽

pp. 355-365 ◽

Cited By ~ 5

Author(s):

W. A. Taber ◽

L. C. Vining

Keyword(s):

Carbon Source ◽

Carbon Sources ◽

Ergot Alkaloids ◽

Nitrogen Sources ◽

Claviceps Purpurea ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Different Strains ◽

Do So

Isolates of Claviceps purpurea and Claviceps spp. obtained from various geographical areas were compared for their ability to grow and to produce ergot alkaloids in vitro on various carbon and nitrogen sources. While some differences in utilization of carbon sources for growth were found, there was no observed correlation between utilization of carbohydrates and the capacity to produce ergot alkaloids. The amount of alkaloid produced by different strains depended upon both the carbon and nitrogen sources. In general, those cultures capable of alkaloid production were able to do so on more than one carbon source, but the carbon source allowing greatest production differed from one strain to another. Both producing and non-producing strains could utilize succinic acid as a carbon source for growth, but neither could utilize L-tryptophane as a carbon or nitrogen source for growth.

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Effects of Carbon and Nitrogen Sources on Activity of Cell Envelope Proteinase Produced by Lactobacillus plantarum LP69

Acta Universitatis Cibiniensis Series E Food Technology ◽

10.2478/aucft-2019-0002 ◽

2019 ◽

Vol 23 (1) ◽

pp. 11-18

Author(s):

Fangfang Cheng ◽

He Chen ◽

Ni Lei ◽

Meng Zhang ◽

Hongchang Wan

Keyword(s):

Lactobacillus Plantarum ◽

Specific Activity ◽

Cell Envelope ◽

Carbon Sources ◽

Nitrogen Sources ◽

Soluble Starch ◽

Carbon And Nitrogen Sources ◽

Individual Factor ◽

Carbon And Nitrogen ◽

Mrs Broth

Abstract In present study, the effects of carbon sources (glucose, lactose, sucrose, galactose, maltose and soluble starch) and nitrogen sources (casein peptone, whey protein, soy peptone, yeast, tryptone, beef extract and peptone) on activity of cell envelope proteinases (CEP), specific activity, protein content, OD600 value and pH in MRS broth fermented by Lactobacillus plantarum LP69 were investigated by individual factor experiment. The results indicated that carbon and nitrogen sources have significant influence on the activity of CEP and specific activitys of L. plantarum LP69, glucose, maltose, casein peptone and peptone are superior to other selected carbon and nitrogen sources. The optimum concentrations of glucose, maltose, casein peptone and peptone for L. plantarum LP69 are 2%, 2%, 1% and 1%; the activity of CEP are 19.52U/mL, 21.13U/mL, 13.49U/mL and 20.61U/mL, respectively.

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Influence of carbon and nitrogen sources on growth, nitrogenase activity, and carbon metabolism of Gluconacetobacter diazotrophicus

Canadian Journal of Microbiology ◽

10.1139/w04-066 ◽

2004 ◽

Vol 50 (9) ◽

pp. 745-750 ◽

Cited By ~ 19

Author(s):

Noel A Tejera ◽

Eduardo Ortega ◽

Rosa Rodés ◽

Carmen Lluch

Keyword(s):

Amino Acids ◽

Carbon Metabolism ◽

Nitrogenase Activity ◽

Tricarboxylic Acid Cycle ◽

Nitrogen Sources ◽

Symbiotic Association ◽

Gluconacetobacter Diazotrophicus ◽

Direct Oxidation ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen

The effects of different carbon and nitrogen sources on the growth, nitrogenase activity, and carbon metabolism of Gluconacetobacter diazotrophicus were investigated. The amino acids asparagine, aspartic acid, and glutamic acid affected microbial growth and nitrogenase activity. Several enzymatic activities involved in the tricarboxylic acid cycle were affected by the carbon source used. In addition, glucose and gluconate significantly increased the oxygen consumption (respiration rate) of whole cells of G. diazotrophicus grown under aerobic conditions. Enzymes responsible for direct oxidation of glucose and gluconate were especially active in cells grown with sucrose and gluconate. The presence of amino acids in the apoplastic and symplastic sap of sugarcane stems suggests that these compounds might be of importance in the regulation of growth and nitrogenase activity during the symbiotic association. The information obtained from the plant–bacterium association together with the results of other biochemical studies could contribute to the development of biotechnological applications of G. diazotrophicus.Key words: Gluconacetobacter diazotrophicus, endophyte, metabolism, nitrogen fixation.

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